Comparison of the Sensitivity of Freshwater Algae to 6 Pesticides for Paddy Rice

담수조류의 벼재배용 농약 6종에 대한 감수성 비교

  • Bae, Chul-Han (Agricultural Research Center, Hankooksamgong Co. Ltd.) ;
  • Park, Yeon-Ki (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Yeon-Sik (Agricultural Research Center, Hankooksamgong Co. Ltd.) ;
  • Cho, Kyung-Won (Agricultural Research Center, Hankooksamgong Co. Ltd.) ;
  • Lee, Suk-Hee (Agricultural Research Center, Hankooksamgong Co. Ltd.) ;
  • Jung, Chang-Kook (Agricultural Research Center, Hankooksamgong Co. Ltd.)
  • 배철한 (한국삼공(주) 농업연구소) ;
  • 박연기 (농업과학기술원 농산물안전성부) ;
  • 김연식 (한국삼공(주) 농업연구소) ;
  • 조경원 (한국삼공(주) 농업연구소) ;
  • 이석희 (한국삼공(주) 농업연구소) ;
  • 정창국 (한국삼공(주) 농업연구소)
  • Published : 2008.09.30

Abstract

Algal Growth-inhibition tests were conducted with 6 pesticides butachlor GR (5%), butachlor EW (33%), isoprothiolane GR (12%), isoprothiolane EC (40%), diazinon GR (3%) and diazinon EC (34%) to determine their comparative toxicity to three species of freshwater green algae Selenastrum capricornutum, Scenedesmus subspicatus, Chlorella vulgris and one species diatom Nitzschia palea. The test species were exposed to the test pesticide for 72 hours and the toxicity represented $EC_{50}$ value calculated by the reduction in growth rate at 72 hours. The toxicity of EC or EW formulation to green algae and diatom was higher than the toxicity of GR formulation at all test in this study. Also, the toxicity of EC formulation was higher than the toxicity technical pesticide. These results indicate that the types of pesticide formulation may affect on their toxicity on algae and the green alga, S. capricornutum and S. subspicatus were more sensitive than C. vulgris. Also, the sensitivity of the diatom, N. palea showed equal or lower than S. capricornutum, S. subspicatus but more sensitive than C. vulgris.

벼재배용 농약의 담수 조류에 대한 독성과 감수성을 조사하고자 4종의 담수 조류를 이용하여 생장저해시험을 실시하였다. 시험농약은 벼재배용으로 사용되는 butachlor 5% GR, butachlor 33% EW, isoprothiolane 12% GR, isoprothiolane 40% EC, diazinon 3% GR, diazinon 34% EC이며, 시험농약을 담수 녹조류 3종(Selenastrum capricornutum, Scenedesmus subspicatus, Chlorella vulgris)과 규조류 1종(Nitzschia palea)에 72시간 노출시켜 그 생장률을 조사하였고 생장저해율을 이용하여 50% 영향농도($EC_{50}$)를 산출하였다. 시험 결과, 모든 시험농약에서 유제와 유탁제가 입제보다 담수 조류에 대한 독성이 높았으며, 특히 유제의 경우 농약 원제보다도 높은 독성이 나타났다. 이러한 결과로 농약 제제의 특성에 따라 수조류에 대한 독성이 높아지거나 낮아지고 있음을 알 수 있었다. 그리고 시험종간 감수성에 있어서는 대부분의 시험농약에서 녹조류인 S. capricornutum와 S. subspicatus는 비슷한 수준의 감수성을 나타내고 있는 반면 C. vulgris의 감수성은 확연히 낮았다. 규조류인 N. palea의 농약에 대한 감수성은 S. capricornutum와 S. subspicatus에 비해 다소 낮거나 비슷하였으나, C. vulgris에 비해 감수성이 높게 나타났다.

Keywords

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