• Title/Summary/Keyword: growth conditions

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Migration Patterns of Brachymystax lenok tsinlingensis Using Radio Tags in the Upper Part of the Nakdong River (Radio tag을 이용한 낙동강 상류에 서식하는 열목어의 이동양상)

  • Yoon, Ju-Duk;Jang, Min-Ho
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.58-66
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    • 2009
  • The telemetry of eight adult manchurian trouts (Brachymystax lenok tsinlingensis) in the upper part of the Nakdong River, which is the southern limit of distribution of manchurian trout on the Korean peninsula, was used to examine migration patterns and evaluate characteristics of over-wintering and the spawning season between December, 2007 and May, 2008. Based on the tracking data, the tagged fish showed a limited migration between adjacent pools, moving only up to $8.6m\;day^{-1}$ during the winter season (December to February). Hydraulic conditions of over-wintering pool areas were, ca. 1m depth, slow moving surface water with areas of sand and gravel. The migration of tagged individuals was successful, moving up to $96.2m\;day^{-1}$ during the spawning season. Two tagged individuals (BL4, BL6) exhibited upstream migration, whereas others showed downstream movements. The timing of upstream migration of the two individuals was consistent with an increasing phases of water level and discharge. The fishes migrating toward the down stream moved to the wide pool areas downstream, where they spent the summer season for the growth.

Helixor A Inhibits Angiogenesis in vitro Via Upregutation of Thrombospondin-1 (Helixor A는 시험관 내에서 thrombospondin-1의 상승조절을 통해 신혈관생성을 억제한다.)

  • Yeom Dong-Hoon;Hong Kyong-Ja
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.895-903
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    • 2005
  • Thrombospondin-1 (TSP-1), a negative regulator in tumor growth and angiogenesis, is cell-type specifically regulated under pathological conditions or by extracellular stimuli, and the regulation of TSP-1 gene expression is important for developing new approaches in tumor therapy. Mistletoe is a parasitir plant that have been used for immunomodulation and antitumor therapy. Helixor A is an aqueous part of mistletoes extract. Here we showed that TSP-1 expression was significantly induced at both mRNA and protein levels in the Hepatocarcinorna cell line (Hep3B) and primary bovine endothelial cell line (BAE) exposed to Helixor A. Our promoter analysis confirmed that the expression of TSP-1 gene was regulated by Helixor A at the transcriptional level. In cell invasion assay, the conditioned media obtained from treatment of these cells significantly reduced the number of invasive cells and also inhibited capillary-like tube formation of BAE cells on Matrigel. Moreover, the inhibitory efforts of the conditioned media on cell invasion and tube formation were reversed by blocking with anti-TSP-1 neutralizing antibodies, suggesting that TSP-1 is involved in Helixor A-indured antiangiogenic effect. Taken together, our results suggest that Helixor A have an antiangiogenic effects through upregulation of TSP-1.

Screening of Lactic Acid Bacteria for Strong Folate Synthesis and Optimization of Fermentation (고엽산 생산능의 유산균 탐색 및 발효 조건 최적화)

  • Du, Kyung Min;Park, Se Jin;Park, Myung Soo;Ji, Geun Eog
    • Korean Journal of Food Science and Technology
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    • v.46 no.3
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    • pp.328-333
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    • 2014
  • Folate is a water-soluble vitamin B that is required for the synthesis of amino acids and nucleic acids. It plays an important role in cell division and cell growth in several living organisms. The purpose of this study was to screen strong folate-synthesizing bacteria and to optimize their culture conditions for folate production. Folate production was quantified by microbiological assays by using folate-dependent strain Lactobacillus rhamnosus KCTC 3237. Folate derivatives were identified by LC-MS/MS. Of the 65 strains of bifidobacteria and lactobacilli tested, L. plantarum Fol 708 demonstrated the greatest ability to produce folate. Its optimal pH for folate production was 5.5 in a pH-controlled, lab-scale fermenter. Coculturing L. plantarum Fol 708 with L. brevis GABA 100 in a milk medium enhanced the level of folate produced in comparison to culturing L. plantarum Fol 708 alone.

Effect of Cultivation Method, Harvest Season and Preservative Solution on the Quality and Vase Life of Cut Rose 'Rote Rose' (롯데로제 장미의 재배방법, 수확시기 및 보존제 종류가 절화 품질과 수명에 미치는 영향)

  • Cho, Mee Sook;Hwang, Seung Jae;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.71-77
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    • 2001
  • Experiments were conducted to evaluate quality and vase life of cut rose 'Rote Rose' cultivated in soil or hydroponically in rockwool. Rose flower stems harvested in commercial greenhouses in Kimhae on May 27 and June 14, 1998 were transported for about two hours to a laboratory and recut in water to an uniform stem length of 45cm. The rose flowers harvested on the same day were displayed at a density of $10cm{\times}10cm$ and were subjected to the same environmental conditions in a growth chamber. The stems were put in four different preservative solutions, 0.5% Chrysal RVB, BS (2% sucrose+200ppm 8HQS+0.3% Chrysal RVB), Sonk1 (BS+0.1mM ethionine), and double distilled $H_2O$. Flower stems harvested on May 27 were displayed at $18{\pm}1^{\circ}C$, RH 60-70%, and light intensity of 220lux provided by fluorescent lamps for $16h{\cdot}d^{-1}$. Flower stems harvested on June 14 were displayed at $25{\pm}1^{\circ}C$, RH 70-80%, and light intensity of 220lux provided by fluorescent lamps for $16h{\cdot}d^{-1}$. Fresh weight and flower diameter were affected by cultivation method, and were greater in hydroponically-grown roses than in soil-grown roses. Among the preservative solutions, BS and Sonk1 were superior to Chrysal RVB in terms of prolonging vase life. Vase life extension in Chrysal RVB, BS and Sonk1 over the control was about one day in both display temperatures. At $18^{\circ}C$ vase life was maintained for three to four additional days as compared to that at $25^{\circ}C$.

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Breeding of Garden Chrysanthemum Cultivar 'Nuri Ball' (Dendranthema grandiflorum Ramat.) with White Color Petals and Semi-Decorative Type Characteristics (흰색 반겹꽃의 화단국화 '누리볼' 육성)

  • Kim, Dong Chan;Choi, Hyun Gu;Pak, Ha Seung;Lee, Young Hye;Won, Mikyung;Jung, Yun Kyung;Lee, Jung-Soo
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.789-795
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    • 2015
  • The new garden chrysanthemum (Dendranthema grandiflorum Ramat.) cultivar 'Nuri Ball' was developed at Yesan Chrysanthemum Experiment Station of Chungcheongnam-do Agricultural Research and Extension Services in 2011. 'Nuri Ball' was bred through a cross between the '02-145-01' line as the female parent with yellow flower color and '02-04-32' as the male plant with white flower color in 2004. Three years of adaptation trials were conducted from 2006 to 2009 under natural conditions. This study compared the external shape type with that of 'White Miri' and conducted ploidy and RAPD (Random amplified polymorphic DNA) marker analyses. These tests showed that 'Nuri Ball' cultivar has its own characteristics compared with the control 'White Miri'. 'Nuri Ball' was a shrub type variety with semi-double flowers of 4.0 cm in width with white petals. It could produce 1025.2 flowers per plant in autumn. Compared with the control 'White Miri', 'Nuri Ball' was similar in terms of shape and color of flowers, but was different in flower size and number. The natural flowering time of 'Nuri Ball' was late September. It had very vigorous growth and an early budding plant. 'Nuri Ball' was demonstrated to be a new cultivar based on ploidy test and RAPD analysis. 'Nuri Ball' is intended for use as a bed chrysanthemum and expected to contribute to farm incomes in landscaping.

Characterization of a Drought-Tolerance Gene, BrDSR, in Chinese Cabbage (배추의 건조 저항성 유전자, BrDSR의 기능 검정)

  • Yu, Jae-Gyeong;Lee, Gi-Ho;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.102-111
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    • 2016
  • The goal of this study was to characterize the BrDSR (Drought Stress Resistance in B. rapa) gene and to identify the expression network of drought-inducible genes in Chinese cabbage under drought stress. Agrobacterium-mediated transformation was conducted using a B. rapa inbred line ('CT001') and the pSL100 vector containing the BrDSR full length CDS (438 bp open reading frame). Four transgenic plants were selected by PCR and the expression level of BrDSR was approximately 1.9-3.4-fold greater than that in the wild-type control under drought stress. Phenotypic characteristics showed that BrDSR over-expressing plants were resistant to drought stress and showed normal growth habit. To construct a co-expression network of drought-responsive genes, B. rapa 135K cDNA microarray data was analyzed to identify genes associated with BrDSR. BrDSR was directly linked to DARK INDUCIBLE 2 (DIN2, AT3G60140) and AUTOPHAGY 8H (ATG8H, AT3G06420) previously reported to be leaf senescence and autophagy-related genes in plants. Taken together, the results of this study indicated that BrDSR plays a significant role in enhancement of tolerance to drought conditions.

Infestation of the Abalone, Haliotis Discus Hannai, by the Polydora under Intensive Culture Conditions in Korea (우리나라 전복 양식장의 패각 천공성 다모류 감염현황)

  • Won, Kyoung-Mi;Kim, Byeng-Hak;Jin, Young-Guk;Park, Young-Jin;Son, Maeng-Hyun;Cho, Mi-Young;Park, Myoung-Ae;Park, Min-Woo
    • Journal of fish pathology
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    • v.26 no.3
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    • pp.139-148
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    • 2013
  • The genus Polydora(Polychaeta, Spionidae) includes many species well known for their activity as borers. They often become harmful invaders by reducing the growth rate and meat yield of, or inducing the mortality of commercially important mollusck, abalone, Haiotis discus hannai. In 2012, the frequency of the Polydora was observed with 5~99% in live abalone and 5.3~70.3% in dead abalone shells of abalone sea-caged aquaculture system, Korea. There are many nacreous blister in the ventral margin and inner of the infestated abalone by abalone in response to the Polydora. A worm bored two holes in the shell and come in and out for ingestion the organic matters. They are more than 40 mm in length and had outstanding palps with black bars, disc form pygidium and 4 eyespots. This is the first record for the statue of Polydora infestation of sea-caged cultured abalone, Haliotis discus hannai in Korea.

Comparison of The Taste Compounds of Wild and Cultured Eel, Puffer and Snake head (천연산 및 양식산 뱀장어, 복어, 가물치의 맛 성분에 대한 연구)

  • Kim, Hee-Yun;Shin, Jae-Wook;Sim, Gyu-Chang;Park, Hee-Ok;Kim, Hyun-Sook;Kim, Sang-Moo;Cho, Jae-Sun;Jang, Young-Mi
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1058-1067
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    • 2000
  • In this study, eel, puffer and snake head fish, which are widely taken and highly valued for processing into baked fish, soup or fish juice were studied by classifying them into wild fish and cultured fish, and evaluated in terms of taste. The samples were gathered in the area including Kimhae, Samchonpo and Haman in Kyungsangnam-do, Kangsu-Gu in Pusan and Bukcheju-Gun in Cheju-do. Proximate compositions, the content of nucleotides and their related compounds, total and free amino acids, organic acids and fatty acids of the fishes were analysed. Generally, as for the compositions, wild fish had a higher crude fat content and a lower moisture content as compared with cultured fish, while there was no great difference between them in terms of crude protein and ash contents. Nucleotides and their related compounds including ATP, ADP, AMP, IMP, HxR, Hx were detected, and the amounts of which were nearly the same with respect to the growth conditions. IMP content was high in all of the samples, while ATP content was extremely low. Total 17 amino acids were detected from the samples, and most of the samples had high contents of glutamic acid, aspartic acid, lysine, leucine, glycine, alanine and arginine, and low contents of cystein, isoleucine and methionine. As for the other amino acids, generally same level of contents were detected for each samples. In cases of eel and snake head fish, the wild fish had higher total amino acid contents in comparison with cultured fish. And the result to the contrary was obtained for the case of puffer. Sample fishes had nearly the same compose distribution of free amino acid though the contents of which were a little different according to the samples. Each sample had high contents of taurine, hydroxyproline, glutamic acid, glycine, cystathionine, ${\beta}-aminoisobutyric$ acid and lysine. Total organic acid contents of cultured fish was higher than that of wild fish. All fishes commonly had high contents of lactic acid, iso-valeric acid, oxalic acid and fumaric acid. High levels of $C_{16:0},\;C_{16:1},\;C_{18:1(cis)},\;C_{18:3},\;and\;C_{22:6}$ were shown in all of the samples and ${\omega}-3$ polyunsaturated fatty acid content of cultured fish was higher than that of wild fish, and as for the essential fatty acid, wild fish has higher content in comparison with cultured fish.

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Detection of the Factors Related to spermatization in Sclerotinia trifoliorum -I. Course of Fertilization (Sclerotinia trifoliorum의 Spermatization에 관여하는 요인(要因)의 검색(檢索) -I. 균(菌)의 수정과정(受精過程))

  • Uhm, Jae Youl;Kim, Young Tae
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.127-133
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    • 1987
  • The process of fertilization and changes in anatomical structure of sclerotia during the apothecial formation in Sclerotinia trifoliorum, the causal fungus of sclerotial rot of forage legumes, were investigated. The time of fertilization could be estimated with fair accuracy by the sequencial spermatization of the sclerotia which kept at 15C in saturated moisture. In the case of one strain used in this experiment, fertilization between the sclerotia and spermatia were estimated to take place at around 18days after the sclerotia were placed under the conditions for apothecial induction (15C, saturated moisture). The fertilizable state was maintained for about 45 days and the spermatization thereafter did not induce the apothecial formation. When the sclerotia reached fertilizable state, a number of interwoven hyphal nests were developed within the medulla of sclerotia, regardless of the sexuality of the cultures. Comparing the process of multiplication and growth of the hyphal nests in homothallic and heterothallic culture, they were identified as ascogonium. These ascogonia were persisted for about 45 days. This observation was well coincided with the duration of fertilizable state elucidated by the sequencial spermatization experiment.

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A Study on the Relationship between Franchise Firm's Supervisors Job Insecurity and Organizational Effectiveness: The Moderating Effect of Self-Efficacy and Trust in Manager (슈퍼바이저의 고용불안정성이 조직유효성에 미치는 영향에 관한 연구: 자기효능감과 신뢰의 조절효과)

  • Cho, Joon-Sang
    • Journal of Distribution Science
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    • v.13 no.1
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    • pp.35-46
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    • 2015
  • Purpose - This paper attempted to investigate the relationships among the perception of job insecurity and organizational effectiveness, and it examines these relationships while considering the moderating effect of trust in the manager and self-efficacy. Job insecurity is an independent variable, organizational effectiveness aspects (job satisfaction, organizational commitment, and turnover intention) are dependent variables, and trust and self-efficacy are moderators. Research design, data, and methodology - Regression analysis was used to verify the effects. The measurement items were already deemed as reliable and valid in the previous study, but for this purpose, some modifications were made. To examine the model, this study relied on the samples chosen from Korean employees in the six franchise business firms. The survey was conducted on 288 workers. Each question is based on a 5-point Likert type scale. The data were analyzed using SPSS 18.0. Results - The results of the study are summarized as follows. First, job insecurity has an influence on organizational effectiveness aspects (negatively on job satisfaction, negatively on organizational commitment, and positively on turnover intention). Second, analyzing the moderating effect of trust, trust in manager is mediated between job insecurity and organizational effectiveness aspects (on organizational commitment and on turnover intention). However, employees' trust in manager had no significant moderating effect on the job insecurity-job satisfaction relationship. Third, self-efficacy is mediated between job insecurity and organizational effectiveness aspects (on job satisfaction, on organizational commitment, and on turnover intention). Conclusions - First, it is necessary to be aware of the seriousness of employees' job insecurity in franchise firms, which have played a pivotal role in national economic growth, and to create a detailed plan for reducing insecurity and actively implementing it. To this end, the franchise companies should implement efficiency efforts such as obtaining an appropriate capacity of equipped personnel and a training program. Second, there are moderating effects of self-efficacy and trust in the relationship between job insecurity and organizational effectiveness. Depending on business conditions, stability and instability can only be experienced in the organizations of franchise companies. This can give rise to instability in employment. Therefore, it is necessary to develop and utilize a system that can be minimized with a change in the new awareness of employment instability. After all, a good leader (manager) must accumulate personal trust and build up a core competence that is necessary to become competent in the field himself. Even if you lack the material resources to improve performance, if the leader with the core competencies (e.g., technical/management /organization/marketing/design) can gain the trust of the supervisor, you can get a good organizational performance. Therefore, you should foster a healthy organizational culture through education such as leadership training and employee training to be built on trust between the leaders and the employees. In addition, you need to focus on HRD&M training in order to increase the self-efficacy of the supervisor.