• Title/Summary/Keyword: growth conditions

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Effect of $CO_2$ Enrichment on Photosynthetic Rates, Enzyme Activity rind End Products of toro Poplar Clones, 1-214 (Populus euramericana) and Peace (P. Koreana x P. trichocarpa)

  • Shin-Young Park;Akio Furukawa
    • Journal of Environmental Science International
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    • v.1 no.1
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    • pp.51-59
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    • 1992
  • Two comparative poplar clones (I-214: Populus euramerinm, Peace: P koreana x p. trihocarpa) were exposed to two $CO_2$ concentrations (350 or 2, 000 ${\mu}L L^{-1} CO_2$) for 21 days. When both poplar clones were compared at growth conditions, the net photosynthetic rate ($P_N$) in $CO_2$-enriched ($2, 000{\mu}L L^{-1} CO_2 = C_{2, 000}$) plants become about 50-60% higher than that of 350 ${\mu}L L^{-1} CO_2 (=C_{350}$ Plants on 7 days treatment. But the enhancement of PN by high $CO_2$ was not maintained throughout all the experimental period. At 21 days, there was no difference of photosynthetic rates between $C_{350}$ and $C_{2000}$ plants. In contrast with photosynthesis, the response of leaf conductance to the elevated $CO_2$ concentration was very different between I-214 and Peace. During all experimental period, leaf conductance ($g_{s}$) of $C_{2000}$ plants is 50% lower than that of the $C_{350}$ plants for I-214, while there is no difference of gs between the plants of $C_{350}$ and $C_{2, 000}$ for Peace. The results of gs in Peace indicate that decreased photosynthetic rate after 21 days in $C_{2, 000}$ Plants for two poplar clones is possibly due to non-stomatal factors. To investigate the non-stomatal factors, starch accumulation and ribulose-1, 6-bisphosphate carboxylase (RuBPCase) were measured. We found significant accumulation of starch in two poplar clones exposed to high $CO_2$, especially starch of I-214 in $C_{2, 000}$ become 3.5 times higher than in $C_{350}$ plants at 21 days. This suggests that high proportion of photosynthates was directed into starch. After 21 days, the activity of ribulose-1, 6-bisphosphate carboxylase of $C_{2, 000}$ plants become decreased in 40-50% compared with that of the $C_{350}$ plants. Two poplar clones show the same trend to RuBPCase declines under high $CO_2$ concentration, although the decline is more significant for I-214. The results reported here suggest that starch accumulation and decreased RuBPCase activity in $C_{2, 000}$ plants can be partly ascribed to the loss of photosynthetic efficiency of high $CO_2$-grown poplar plants.

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Effect of Freezing Conditions on the Formation of Ice Crystals in Food during Freezing Process (식품의 동결중에 생성되는 빙결정에 미치는 동결조건의 영향)

  • 공재열;김정한;김민용;배승권
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.2
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    • pp.213-218
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    • 1992
  • The reaching time to the freezing point was to be fast in the order of 2% agar gel, 5% agar gel, 20% gelatin gel, pork, respectively. The freezing time and the passing time through the zone of the maximum ice crystal formation had linear relationship with the coolant temperature. The average diameter d$_{p}$ of ice crystal in a soybean protein gel and the moving of freezing front were represented an inverse proportion, and the moving velocity of freezing front was shown as 3.4$\times$10$^{-6}$ $\textrm{cm}^2$/sec from predicted theoretical formula. This value was very close to experimental results. The storage temperature did not give any influences for the growth of ice crystal in inside soybean protein gels during freezing conservation. The relationship between freezing condition and structure of freezing front was as follows : (moving velocity of freezing front) : (mass transfer rate of water at freezing point)$\times$(surface area of freezing front).

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Purification and Characterization of Bacillus subtilis JS-17 Collagenase. (Bacillus subtilis JS-17이 생산하는 Collagenase의 정제 및 특성)

  • Lim Kyoung-Suk;Son Shung-Hui;Kang Ho Young;Jun Hong-Ki
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.657-663
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    • 2005
  • Collagenases are generally defined as enzymes that are capable of degrading the polypeptide backbone of native collagen under conditions that do not denature the protein. An extracellular collagenase-producing bacterial strain was isolated from kimchi and identified to be Bacillus subtilis JS-17 through morphological, cultural, biochemical characteristics and 16S rDNA sequence analysis. Optimum culture condition of Bacillus subtilis JS-17 for the production of collagenase was $1.5\%$ fructose, $1\%$ yeast extract, $0.5\%\;K_2HPO_4,\;0.4\%\;KH_2PO_4,\;0.01\%\;MgSO_4\cdot7H_2O,\;0.01\%\; MnSO_4\cdot4H_2O,\;,0.1\%$ citrate and $0.1\%\;CaCl_2$. The production of collagenase was optimal at $30^{\circ}C$ for 72 hr. A collagenase was isolated from the culture filtrate of Bacillus subtilis JS-17. The enzyme was purified using Amberlite IRA-900 column chromatography, Sephacryl S-300 HR column chromatography and DEAE-Sephadex A-50 column chromatography The purified collagenase has an specific activity 192.1 units/mg. The molecular weight of the purified enzyme was estimated to be 28 kDa by SDS-PACE. The purified collagenase has $100\%$ activity up to $55^{\circ}C$.

Effects of Vacuum Container on the Storability of Leftover Tofu (진공용기를 이용한 포장두부의 개봉 후 저장성 연장)

  • Lee, Ji-Youn;Jang, Si-Hun;Lee, Youn-Suk;Park, Su-Il
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.11-15
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    • 2009
  • Commercial tofu was cut and re-packed in a vacuum container, an airtight container and an open tofu tray in an effort to assess the effects of vacuum containers on the shelf life of leftover tofu. The re-packed tofu were stored at $5^{\circ}C$ and $25^{\circ}C$ for the enumeration of total bacteria and coliforms. We also measured changes in color, pH, and opacity. The vacuum retarded the bacterial growth under $10^5$ CFU/mL for up to 12 days at $5^{\circ}C$. The color of the tofu was unaffected by the container type at both storage temperatures, whereas the pH and opacity of the immersion solution were altered less profoundly by storage in the vacuum container at $5^{\circ}C$. However, we noted no significant differences in bacterial growths, pH, and opacity among container types at $25^{\circ}C$. The results of this study showed that the usage of vacuum containers may be a favorable method for the preservation of tofu under refrigerated conditions.

Optimization and characterization of biodiesel produced from vegetable oil

  • Mustapha, Amina T.;Abdulkareem, Saka A.;Jimoh, Abdulfatai;Agbajelola, David O.;Okafor, Joseph O.
    • Advances in Energy Research
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    • v.1 no.2
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    • pp.147-163
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    • 2013
  • The world faces several issues of energy crisis and environmental deterioration due to over-dependence on single source of which is fossil fuel. Though, fuel is needed as ingredients for industrial development and growth of any country, however the fossil fuel which is a major source of energy for this purpose has always been terrifying thus the need for alternative and renewable energy sources. The search for alternative energy sources resulted into the acceptance of a biofuel as a reliable alternative energy source. This work presents the study of optimization of process of transesterification of vegetable oil to biodiesel using NaOH as catalyst. A $2^4$ factorial design method was employed to investigate the influence of ratio of oil to methanol, temperature, NaOH concentration, and transesterification time on the yield of biodiesel from vegetable oil. Low and high levels of the key factors considered were 4:1 and 6:1 mole ratio, 30 and $60^{\circ}C$ temperatures, 0.5 and 1.0 wt% catalyst concentration, and 30 and 60 min reaction time. Results obtained revealed that oil to methanol molar ratio of 6:1, tranesetrification temperature of $60^{\circ}C$, catalyst concentration of 1.0wt % and reaction time of 30 min are the best operating conditions for the optimum yield of biofuel from vegetable oil, with optimum yield of 95.8%. Results obtained on the characterizzation of the produced biodiesel indicate that the specific gravity, cloud point, flash point, sulphur content, viscosity, diesel index, centane number, acid value, free glycerine, total glycerine and total recovery are 0.8899, 4, 13, 0.0087%, 4.83, 25, 54.6. 0.228mgKOH/g, 0.018, 0.23% and 96% respectively. Results also indicate that the qualities of the biodiesel tested for are in conformity with the set standard. A model equation was developed based on the results obtained using a statistical tool. Analysis of variance (ANOVA) of data shows that mole ratio of ground nut oil to methanol and transesterification time have the most pronounced effect on the biodiesel yield with contributions of 55.06% and 9.22% respectively. It can be inferred from the results various conducted that vegetable oil locally produced from groundnut oil can be utilized as a feedstock for biodiesel production.

The Effects of Levelers on Electroplating of Thin Copper Foil for FCCL (전기도금법을 이용한 FCCL용 구리박막 제조시 레벨러의 영향 연구)

  • Kang, In-Seok;Koo, Yeon-Soo;Lee, Jae-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.19 no.2
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    • pp.67-72
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    • 2012
  • In recent days, the wire width of IC is narrowed and the degree of integration of IC is increased to obtain the higher capacity of the devices in electronic industry. And then the surface quality of FCCL(Flexible Copper Clad Laminate) became increasingly important. Surface defects on FCCL are bump, scratch, dent and so on. In particular, bumps cause low reliability of the products. Even though there are bumps on the surface, if leveling characteristic of plating solution is good, it does not develop significant bump. In this study, the leveling characteristics of additives are investigated. The objective of study is to improve the leveling characteristic and reduce the surface step through additives and plating conditions. The additives in the electrodeposition bath are critical to obtain flat surface and free of defects. In order to form flat copper surface, accelerator, suppressor and leveler are added to the stock solution. The reason for the addition of leveler is planarization surface and inhibition of the formation of micro-bump. Levelers (SO(Safranin O), MV(Methylene Violet), AB(Alcian Blue), JGB(Janus Green B), DB(Diazine Black) and PVP(Polyvinyl Pyrrolidone) are used in copper plating solution to enhance the morphology of electroplated copper. In this study, the nucleation and growth behavior of copper with variation of additives are studied. The leveling characteristics are analyzed on artificially fabricated Ni bumps.

Tests of Different Erosion Control Blankets for Erosion Control and Plant Growth Enhancement under Simulated Rain and Sunlight (강우 및 태양광 씨뮤레이터를 이용한 침식방지 브랑켓들의 침식방지 효과와 식물성장의 촉진효과 확인을 위한 실험)

  • Chang, Chang-Hark;Urroz, Gilberto E.
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.1 no.1
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    • pp.133-140
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    • 1998
  • The following conclusions are based upon data collected and visual observations made during the performance of the tests : 1. The performance of the erosion control products tested was for a particular set of conditions, and may be expected to differ if any or all of the test parameters were to be changed. If even just one parameter is changed from one test to the next, the results can be expected to be different. 2. Due to the fact that only two replications of each product were tested, we believe that the results presented herein are indicative only and not conclusive. 3. The ECB SC produced the least amount of soil erosion followed by ECB S, ECB C, and Coir No. 2, in that order. 4. All of the erosion control blankets tested significantly reduced soil erosion rates with respect to the bare soil controls. 5. The ECB S produced the smallest water runoff rate, followed closely by ECB SC. Next in order were ECB C and Coir No. 2. 6. All of the erosion control blankets reduced the water runoff rate with respect to the bare soil control. 7. Mesh 2cm There was not much difference in plant height for the four erosion control blankets and the bare soil control plots. the ECB S produced slightly taller plants than the rest of the materials tested. 8. The four erosion control blankets(ECB C, ECB SC, ECB S, and Coir No. 2) produced a larger plant mass than the bare soil plots. The difference between the plant mass for the four erosion control blankets, however, is minimal. 9. The ECB C produced the least percentage of lost seed and the largest percentage of germinating seed. 10. The ECB SC had the second smaller percentage of seed lost, followed closely by ECB S, and then by Coir No. 2. 11. All erosion control blankets had a smaller percentage of seed lost than the bare soil control plots. 12. The ECB C had the second largest percentage of germinating seed, followed closely by ECB SC and Coir No. 2. 13. All erosion control blankets had a larger percentage of germinating seed than the bare soil control plots. 14. The ECB C had the smallest percentage of non-germinating seed, followed by ECB S, Coir No. 2, and ECB SC, in that order. 15. All erosion control blankets had smaller percentages of non-germinating seed than the bare soil control plots.

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Perspectives and Strategies of Production of Miscellaneous Crops and Animal Feeds (잡곡 및 사료 생산 수급전망과 대책)

  • Jung Seung Keun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1998.10a
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    • pp.266-287
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    • 1998
  • Environmental conditions and expected profit are the major criteria to select crops to be cultivated in any region. Traditionally, miscellaneous crops have been cultivated as alternative crop in case of unfavorable climate or supplementary crop in marginal lands. Since the successful breeding of high yielding varieties of staple crops and development of cultural techniques in 1970s in Korea, production of miscellaneous crops decreased markedly due to the rapid commercialization of agriculture and increased productions of horticultural crops, although yields have been doubled during the last $2\~3$ decades. On the other hand, animal husbandry has been developing remarkably parallel with national economic development and the increased consumption of animal products. As a result, imports of feed grains such as corn have increased rapidly, while roughage production became lower than demand. Among miscellaneous crops, corn production increased markedly through the development of hybrids and due to its importance as silage crops. Acreage of corn production including grains, fresh corns and feeding is about 120,000 ha, while acreage of buckwheat is about 5,000 ha and those of other miscellaneous crops decreased to a level of about 1,000 ha. Although miscellaneous crops have low yield potential and are unprofitable due to low price and imports of cheap foreign products, they should be kept to cultivate. Miscellaneous crops are important components that maintain diversity among upland crops as well as alternative crops in case of unfavorable climate. The low yield potential of miscellaneous crops might be due to lack of efforts to breed high yielding varieties and to develope cultural techniques. Continuous investment in research, exploitation of new utilization for miscellaneous crops as sustainable crop, honey crop and sightseeing crop, and development of healthy and special foods will promote their cultivation. Animal feeding in Korea depends mainly on formula feeds. As the number of animals increases to meet demand of animal products, there is no alternative way but to import grains to feed them. But roughage production, which is necessary for normal growth of ruminant animals, should be increased. However, lack of arable land and pasture land limits the production of good roughages. It is estimated that number of course for meat and milk production will be $2.5{\~}2.6$ million and total of $6.2{\~}7.5$ million tons of roughages should be produced. This implies that more than 1 million tons of roughage are needed, although pasture land, upland for forage crops, forage crops after rice cultivation and rice straw are utilized. Therefore, new reclamation of pasture land, increased roughage production in cultivating land, increased cultivation of forage crops after rice, more utilization of rice straw and active exploration of indigenous plant species as roughage resources should be promoted

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Studies on the Varietal Differences of Tolerance to Cold Damage in Seedling Stage of the Rice Plant (수도 유묘기 내냉성의 품종간 차이에 관한 연구)

  • Chung-Ik Cho;Seong Kuk Bae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.1
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    • pp.35-42
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    • 1976
  • In Korea, two types of rice varieties are being cultivated widely, Japonica and Japonica-Indica cross. Generally speaking, the latter shows weak cold tolerance than the former and so brought about many problems in cultural practices. This study was conducted to investigate the effect . of various temperature conditions on rice seedlings growth, especially the contents of chlorophyll and the percentage of nitrogen. And the result obtained indicated the varieties of Japonica-Indica intend to weak cold tolerance than Japonica varieties.

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Nitrogen and Phosphorus Removal Characteristics of a New Biological Nutrient Removal Process with Pre-Denitrification by Pilot Scale and Computer Simulation Program (선단무산소조를 이용한 영양소제거공정(Bio-NET)의 질소·인 제거 특성)

  • Oh, Young-Khee;Oh, Sung-Min;Hwang, Yenug-Sang;Lee, Kung-Soo;Park, No-Yeon;Ko, Kwang-Baik
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.1
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    • pp.121-132
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    • 2000
  • This study is to investigate the performance of a new BNR process using predenitrification scheme focusing on nitrogen removal and the possibility of adapting a computer simulation scheme in BNR process development. By using a pre-denitrification basin, higher $COD/NO_3-N$ ratio could be sustained in this BNR process. The results of the investigation showed a SDNR value of 9.04mg/gMv/hr. In the anoxic tank, the average value of SPRR of 6.25mgP/gMv/hr was observed to be very sensitive to SCOD load of influents. By calibrating internal parameters (stoichiometric and kinetic parameters) of the simulation model, the results of simulation for various BNR processes gave good agreement with observed data. The major adjustment was given with three parameters, maximum specific growth rate of heterotrophic biomass, short chain fatty acid (SCFA) limit, and phosphorous release rate. With the series of simulations on varying operational conditions, the simulation by computer program can be a useful tool for process selection, and design and operation of municipal wastewater treatment plant.

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