• Title/Summary/Keyword: growth conditions

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Control of histamine-forming bacteria by probiotic lactic acid bacteria isolated from fish intestine (생선 내장으로부터 분리된 프로바이오틱 유산균에 의한 히스타민 생산균의 제어)

  • Lim, Eun-Seo;Lee, Nahm-Gull
    • Korean Journal of Microbiology
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    • v.52 no.3
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    • pp.352-364
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    • 2016
  • In this study, we examined in vitro the potential probiotic properties of lactic acid bacteria (LAB) obtained from the fish intestine and their ability to degrade histamine through the production of diamine oxidase (DAO) enzymes and bacteriocin. Among 97 LAB strains isolated from the intestine of croaker, flatfish, pollack, and rockfish, CIL08, CIL16, FIL20, FIL31, PIL45, PIL49, PIL52, and RIL60 isolates exhibited excellent survival rates under simulated gastrointestinal tract conditions, high adhesion ability to HT-29 epithelial cells, and resistance to the antibiotics such as amoxicillin, ampicillin, erythromycin, penicillin G, streptomycin, tetracycline, or vancomycin. In addition, these strains did not produce histamine in decarboxylating broth containing histidine. In particular, 4 strains (CIL08, FIL20, PIL52, and RIL60) that may produce DAO were significantly able to degrade histamine. The bacteriocins produced by FIL20, FIL31, and PIL52 LAB inhibited the growth and histamine production of Enterococcus aerogenes CIH05, Serratia marcescens CIH09, Enterococcus faecalis FIH11, Pediococcus halophilus FIH15, Lactobacillus sakei PIH16, Enterococcus faecium PIH19, Leuconostoc mesenteroides RIH25, or Aeromonas hydrophilia RIH28. Histamine-producing strains isolated from fish intestine were found to reduce histamine accumulation during co-culture with CIL08, FIL20, PIL52, and RIL60 LAB showing histamine degradation or bacteriocin production ability. The probiotic strains preventing histamine formation were identified as Pediococcus pentosaceus CIL08, Lactobacillus plantarum FIL20, Lactobacillus paracasei FIL31, Lactobacillus sakei PIL52, and Leuconostoc mesenteroides RIL60 with high similarity based on 16S rRNA gene sequencing.

Extreme Weather Frequency Data over 167 Si-gun of S. Korea with High-resolution Topo-climatology Model (고해상도 소기후모형을 이용한 국내 167개 시·군별 이상기상 발생빈도 자료)

  • Jo, Sera;Shim, Kyo Moon;Park, Joo Hyeon;Kim, Yong Seok;Hur, Jina
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.3
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    • pp.164-170
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    • 2020
  • The weather conditions, such as temperature, precipitation, and sunshine duration, play one of the key roles in Agriculture. In particular, extreme weather events have crucial impacts on growth and yields of crops. This study estimates statistics of extreme weather events in 167 Si-gun over South Korea derived from high-resolution(30 and 270m) topo-climatology model for key three meteorological variables(temperature, precipitation and sunshine duration). It is shown that the characteristic of each extreme weather frequency in the topo-climatology model is in good agreement with observation from Korean Meteorological Administration's Automatic Surface Observing System. Moreover, it is possible to analyze the statistics of extreme weather more realistically because this data can cover the weather at not-observed regions. Hence, this data is expected to be used as baseline data for assessing vulnerability to extreme weather and politic decisions for damage reduction in agricultural sector.

The Changes and Present Status of Education in Clothing Departments at Vocational High Schools (전문계 고등학교 의상과 교육과정에서의 교육목표 및 내용에 관한 연구)

  • Yoo, Hye-Ja;Chunhg, Mee-Kyung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.34 no.2
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    • pp.371-384
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    • 2010
  • This research investigates the historical changes of the educational system for the clothing departments at vocational high schools. According to the growth of the fashion industry, employment potential in the clothing departments of the vocational high schools, numerical changes in the educational statistics, and curriculums were investigated by papers, reports, and statistic data. The statistic data were derived from the 'Center Educational Statistics and Information', 'School Information' (Hak-gyo Alimi), 'Korean Statistical Information Services' and internet home pages of vocational high schools. Technical service workers that graduate from clothing departments and vocational high schools have limited competitiveness in a thriving fashion industry field. Highly developed fashion industries require more professional workers in fashion design, fashion marketing, and fashion materials. Compared to 1994, when 35 classes were conducted in 5 vocational high schools, 69 classes are now conducted at 15 schools. They have over 2,000 students and produce over 600 graduates. However, 222 credits of the $1^{st}$ curriculum in 1958 went down to 82 credits in the 2007 revised curriculum to complete the credits of clothing departments. The 15 courses of the 1st curriculum fell to 5 courses in the 2007 revised curriculum on the number of major subjects. It is a fundamental problem that major courses in clothing education have been excessively reduced despite the demand for of highly specialized workers in the fashion industry. In the view of operating conditions of those schools, there were several problems such as the wide gaps in the curriculum, limited equipment, the supply of teachers, and counseling on the choice of college or a career. In conclusion, the following measures are required: 1) the increase of complement credits in major subjects and renovation of curriculum, 2) national substantial support to change the social circumstance concerning vocational education and occupational consciousness, 3) operation of credit approving systems in universities and colleges for students that have completed the same courses at vocational high schools, 4) designing realistic programs for vocational education and cooperation systems with corporate enterprises.

lonizing Radiation Hormesis in Crops (저선량 전리방사선에 의한 작물의 활성증진)

  • Kim, Jae-Sung;Lee, Young-Bok
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.76-83
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    • 1998
  • The most remarkable aspect in the hormesis law is that dose of harmful agents can produce effect that are diametrically opposite to the effect found with high doses of the same agent. Minute quantities of a harmful agent bring about very small change in the organism and control mechanisms appear to subjugate normal processes to place the organism in a state of albert and repair. The stimulated organism in more responsive to changes in environmental factors than it did before being alerted. Routine functions, including repair and defense, have priority for available energy and matetial. The alerted organism utilizes nutrients more efficiently, grows faster, shows improved defense, and lives longer. Accelerated germination, sprouting, growth, development, blooming and ripening, and increased crop yield and resistance to disease are found in plants. Another concept supported by the data in that low doses of ionizing radiation provide increased resistance to subsequent high doses of radiation. The hormesis varies with subject plant, variety, state of seed, environmental and cultural conditions, physiologic function measured, dose rate and total exposure. The results of hormesis are less consistently found, probably due to the great number of uncontrolled variables in the experiments. The general dosage for radiation homlesis in about 100 (10 to 1,000) times ambient or 100 (10 to 1,000) times less than a definitely harmful dose, but these must be modified to the occasion. Although little is known about most mechanisms of homzesis reaction, overcompensation of repair mechanism is offered as one mechanism.

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Effects of Temperature, Food Concentration, and Shell Size on Filtering Rate and Pseudofeces Production of Unio douglasiae on Microcystis aeruginosa (수온, 먹이농도, 패각 크기가 Microcystis aeruginosa에 대한 말조개의 여과율 및 배설물 생산에 미치는 영향)

  • Lee, Yeon-Ju;Kim, Baik-Ho;Kim, Nan-Young;Um, Han-Yong;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.spc
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    • pp.61-67
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    • 2008
  • This study was conducted to evaluate filtering rate (FR) and pseudofeces production (PFP) of a freshwater filter-feeding bivalve, Unio douglasiae, on a toxic cyanobacterium (Microcystis aeruginosa). The experiments were conducted under the various conditions of water temperature $(5{\sim}35^{\circ}C)$, mussel size $(5.6{\sim}13.3cm)$ and food con centrations $(49{\sim}491{\mu}g\;Chl-{\alpha}L^{-1})$. Among the applied temperature, the maximum FR $(0.41L\;gAFDW^{-1}hr^{-1})$ and PFP (0.47mg $gAFDW^{-1}hr^{-1}$) were observed at 15 and $25^{\circ}C$, respectively. Both weight-based FR and PFP were not correlated with the mussel size, and the values lied in a limited range with some degree of variation. Likewise, no significant relations between FR and PFP was observed in the mussel size. The FR values were negatively correlated with food concentration, while PFP showed positive correlation. Among the applied food concentrations, the maximum FR (0.34L $gAFDW^{-1}hr^{-1}$) and PFP (0.06mg $gAFDW^{-1}hr^{-1}$) appeared in $113{\mu}g\;Chl-{\alpha}L^{-1}$ and $491{\mu}g\;Chl-{\alpha}L^{-1}$, respectively. These results indicate that the grazing of Unio douglasiae are affected by various parameters, and it may be applied as an effective biofilter to inhibit Microcystis bloom under appropriate application. However, further studies on the fate of excreted pseudofeces are needed to understand their possibility of stimulating nuisant algal growth.

Algae and Nutrient Removal by Vegetated Artificial Floating Island (인공식물섬에 의한 조류(Algae)및 영양염류의 제거)

  • Park, Sun-Koo;Cho, In-Ki;Kwon, Oh-Byung;Mun, Jung-Soo;Um, Han-Yong;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.spc
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    • pp.93-98
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    • 2008
  • We investigated the effect on the removal of BOD, SS, TN and TP and algal growth inhibition of Vegetated Artificial Floating Island (VAFI), by examining microorganism activity and nutrient uptake in the batch test of various conditions: (1) Blank (Control group), (2) VAFI of $0.25m^2$, (3) AFI of $0.25m^2$ which has no vegetation, (4) buoyant plate of $0.25m^2$, (5) buoyant plate of $0.25m^2$ with linear media. The proportion of BOD removal in the VAFI, AFI, buoyant plate and buoyant plate with media were 82.7, 80.8, 45.2% and 59.6% respectively. TN removal in the VAFI, AFI and buoyant plate with media were 51.2, 31.7% and 25.1% respectively. TP removal in the VAFI, AFI, buoyant plate and buoyant plate with media were 23.3, 16.7, 10.0% and 13.3% respectively. Chlorophyll-${\alpha}$ removal in the VAFI was 97.9%. The factors of chlorophyll-${\alpha}$ removal in the VAFI accounted for the shading effect of 35.1%, microorganisms activity of 61%, and plant root of 1.8%.

Effect of Surface Sterilization Method on Agrobacterium-mediated Transformation of Field-grown Zoysiagrass Stolon (포장생육 잔디 포복경을 이용한 잔디 형질전환에 있어서 살균방법의 영향)

  • Ahn, Na-Young;Alam, Iftekhar;Kim, Yong-Goo;Bae, Eun-Ji;Lee, Kwang-Soo;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.100-104
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    • 2013
  • Zoysiagrass (Zoysia japonica Steud.) is an important forage and turfgrass that spreads by stolons and rhizomes. Zoysiagrass stolon can be used directly for Agrobacterium-mediated genetic transformation by exploiting the potential of direct shoot formation. However, surface sterilization of field-grown stolons is difficult and remains to be explored. We developed an effective surface sterilization and culture method using the stolon explant for infection with Agrobacterium tumefaciens. Among various treatments, sequential disinfection in 30% bleach for 15 min followed by 0.1% mercuric chloride for 25 min resulted in the highest number of clean stolons. The efficacy of mercuric chloride was increased under vacuum conditions by incubating at 800 mbar for 5 min. The inclusion of 2.5 mg/l amphotericin B further prevents fungal growth in in vitro cultures. This protocol would speed up the development of transgenic plants by utilizing field-grown stolon nodes.

Factors Affecting the Financial Structure of Hospitals in Korea (병원의 재무구조에 영향을 미치는 요인)

  • 최만규;문옥륜;황인경
    • Health Policy and Management
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    • v.12 no.2
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    • pp.43-75
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    • 2002
  • This study focuses on the factors that make the financial structure of hospitals in Korea different, and on recommended courses of action that could be very helpful to hospitals in maintaining a sound financial structure. Data used in this study were collected from 132 hospitals with complete general data of present conditions as well as financial statements. They were chosen from the 174 hospitals that passed the standardization audit undertaken by the Korean Hospital Association from 1996 to 2000 for the purpose of accrediting training hospitals. The dependent variable in this study is financial structure. It consists of liabilities as against total assets (total liabilities to total assets, short-term liabilities to total assets, long-term liabilities to total assets, short-term borrowings to total assets, long-term borrowings to total assets). The independent variables are ownership type, hospital type, location, whether or not a representative is a director of the hospital, the possibility of changing a hospital director, bed size, period of establishment, asset structure, profitability, growth, tax shields, business risk, competition. The factors that appear to have the strongest impact on the liabilities to total assets of all the hospitals sampled are ownership type, hospital type, profitability, tax shields, and business risk. It was found that not-for-profit private hospitals and for-profit private hospitals have more liabilities than public hospitals, and tertiary medical institutions have less liabilities than the secondary general hospitals. Moreover, hospitals earning more at the expense of high business risk have a distinct tendency to lower liabilities. Concerning the current ratio, it was found that factors such as ownership type, hospital type, period of establishment, asset structure, and business risk are the more significant variables. The current ratio of public hospitals is higher than that of both not-for-profit private hospitals and for-profit private hospitals, and the current ratio of tertiary medical institutions is higher than that of general hospitals. As business risk is higher in hospitals compared to other businesses, the current ratio becomes higher; this is because it is assumed that for fear of bankruptcy, hospitals lessen liabilities to total assets. On the other hand, as hospitals become older, the fixed assets to total assets become lower. It is remarkable that in hospitals, the factors affecting liabilities to total assets have an opposite regression coefficient sign against factors affecting current ratio. It brings out the same results borne out by the old financial theories and researches, in which a lot of the liabilities of hospitals are considered as the cause of worsening liquidity. Therefore, it is very important for hospitals to maintain a sound financial structure in order to survive using the rational acquisition and maintenance of capital.

Regional Inequalities in Healthcare Indices in Korea: Geo-economic Review and Action Plan (우리나라 보건지표의 지역 격차: 지경학적 고찰과 대응방안)

  • Kim, Chun-Bae;Chung, Moo-Kwon;Kong, In Deok
    • Health Policy and Management
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    • v.28 no.3
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    • pp.240-250
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    • 2018
  • By the end of 2017, in a world of 7.6 billion people, there were inequalities in healthcare indices both within and between nations, and this gap continues to increase. Therefore, this study aims to understand the current status of regional inequalities in healthcare indices and to find an action plan to tackle regional health inequality through a geo-economic review in Korea. Since 2008, there was great inequality in life expectancy and healthy life expectancy by region in not only metropolitan cities but also districts in Korea. While the community health statistics from 2008-2017 show a continuous increase of inequality during the last 10 years in most healthcare indices related to noncommunicable diseases (except for some, like smoking), the inequality has doubled in 254 districts. Furthermore, health inequality intensified as the gap between urban (metropolitan cities) and rural regions (counties) for rates of obesity (self-reported), sufficient walking practices, and healthy lifestyle practices increased from twofold to fivefold. However, regionalism and uneven development are natural consequences of the spatial perspective caused by state-lead developmentalism as Korea has fixed the accumulation strategy as its model for growth with the background of export-led industrialization in the 1960s and heavy and chemical industrialization in the 1970s, although the Constitution of the Republic of Korea recognizes the legal value of balanced development within the regions by specifying "the balanced development of the state" or "ensuring the balanced development of all regions." In addition, the danger of a 30% decline or extinction of local government nationwide is expected by 2040 as we face not only a decline in general and ageing populations but also the era of the demographic cliff. Thus, the government should continuously operate the "Special Committee on Regional Balanced Development" with a government-wide effort until 2030 to prevent disparities in the health conditions of local residents, which is the responsibility of the nation in terms of strengthening governance. To address the regional inequalities of rural and urban regions, it is necessary to re-adjust the basic subsidy and cost-sharing rates with local governments of current national subsidies based mainly on population scale, financial independence of local government, or distribution of healthcare resources and healthcare indices (showing high inequalities) overall.

Experimental Study of the Heat Transfer Rate of the Plate Fin-Tube Condenser for a Household Refrigerator (냉장고용 판형 핀-관 응축기의 열전달 성능에 관한 실험적 연구)

  • Son, Young-Woo;Lee, Jang-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.8
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    • pp.4802-4808
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    • 2014
  • A plate-fin tube type heat exchanger has a lighter weight, approximately 30%, than the conventional circular-fin type condenser of household refrigerator. Because the low weight means low cost, it can have significant effects on the growth of related businesses if similar performance can be guaranteed. To check the possibility of the use of such a plate fin-tube condenser, experimental evaluations were performed in this study. Four different condensers including a conventional circular fin-tube condenser were used for the test. A well designed refrigerant supply system was used to supply similar conditions with a refrigerator, and the heat transfer rate and pressure drops of air side were measured precisely. As a result, the plate fin-tube type condensers showed a lower heat transfer rate of more than 13% than the conventional circular fin-tube type condenser, but the air side pressure drop was reduced and the heat transfer per unit weight was increased. Therefore, it shows the possibility of the use of a plate fin-tube type condenser after optimizing the air flow path and increasing the air flow to make a similar heat transfer rate.