• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.100-104
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• 2013

Zoysiagrass (Zoysia japonica Steud.) is an important forage and turfgrass that spreads by stolons and rhizomes. Zoysiagrass stolon can be used directly for Agrobacterium-mediated genetic transformation by exploiting the potential of direct shoot formation. However, surface sterilization of field-grown stolons is difficult and remains to be explored. We developed an effective surface sterilization and culture method using the stolon explant for infection with Agrobacterium tumefaciens. Among various treatments, sequential disinfection in 30% bleach for 15 min followed by 0.1% mercuric chloride for 25 min resulted in the highest number of clean stolons. The efficacy of mercuric chloride was increased under vacuum conditions by incubating at 800 mbar for 5 min. The inclusion of 2.5 mg/l amphotericin B further prevents fungal growth in in vitro cultures. This protocol would speed up the development of transgenic plants by utilizing field-grown stolon nodes.

• Health Policy and Management
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• v.12 no.2
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• pp.43-75
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• 2002

This study focuses on the factors that make the financial structure of hospitals in Korea different, and on recommended courses of action that could be very helpful to hospitals in maintaining a sound financial structure. Data used in this study were collected from 132 hospitals with complete general data of present conditions as well as financial statements. They were chosen from the 174 hospitals that passed the standardization audit undertaken by the Korean Hospital Association from 1996 to 2000 for the purpose of accrediting training hospitals. The dependent variable in this study is financial structure. It consists of liabilities as against total assets (total liabilities to total assets, short-term liabilities to total assets, long-term liabilities to total assets, short-term borrowings to total assets, long-term borrowings to total assets). The independent variables are ownership type, hospital type, location, whether or not a representative is a director of the hospital, the possibility of changing a hospital director, bed size, period of establishment, asset structure, profitability, growth, tax shields, business risk, competition. The factors that appear to have the strongest impact on the liabilities to total assets of all the hospitals sampled are ownership type, hospital type, profitability, tax shields, and business risk. It was found that not-for-profit private hospitals and for-profit private hospitals have more liabilities than public hospitals, and tertiary medical institutions have less liabilities than the secondary general hospitals. Moreover, hospitals earning more at the expense of high business risk have a distinct tendency to lower liabilities. Concerning the current ratio, it was found that factors such as ownership type, hospital type, period of establishment, asset structure, and business risk are the more significant variables. The current ratio of public hospitals is higher than that of both not-for-profit private hospitals and for-profit private hospitals, and the current ratio of tertiary medical institutions is higher than that of general hospitals. As business risk is higher in hospitals compared to other businesses, the current ratio becomes higher; this is because it is assumed that for fear of bankruptcy, hospitals lessen liabilities to total assets. On the other hand, as hospitals become older, the fixed assets to total assets become lower. It is remarkable that in hospitals, the factors affecting liabilities to total assets have an opposite regression coefficient sign against factors affecting current ratio. It brings out the same results borne out by the old financial theories and researches, in which a lot of the liabilities of hospitals are considered as the cause of worsening liquidity. Therefore, it is very important for hospitals to maintain a sound financial structure in order to survive using the rational acquisition and maintenance of capital.

• Health Policy and Management
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• v.28 no.3
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• pp.240-250
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• 2018

By the end of 2017, in a world of 7.6 billion people, there were inequalities in healthcare indices both within and between nations, and this gap continues to increase. Therefore, this study aims to understand the current status of regional inequalities in healthcare indices and to find an action plan to tackle regional health inequality through a geo-economic review in Korea. Since 2008, there was great inequality in life expectancy and healthy life expectancy by region in not only metropolitan cities but also districts in Korea. While the community health statistics from 2008-2017 show a continuous increase of inequality during the last 10 years in most healthcare indices related to noncommunicable diseases (except for some, like smoking), the inequality has doubled in 254 districts. Furthermore, health inequality intensified as the gap between urban (metropolitan cities) and rural regions (counties) for rates of obesity (self-reported), sufficient walking practices, and healthy lifestyle practices increased from twofold to fivefold. However, regionalism and uneven development are natural consequences of the spatial perspective caused by state-lead developmentalism as Korea has fixed the accumulation strategy as its model for growth with the background of export-led industrialization in the 1960s and heavy and chemical industrialization in the 1970s, although the Constitution of the Republic of Korea recognizes the legal value of balanced development within the regions by specifying "the balanced development of the state" or "ensuring the balanced development of all regions." In addition, the danger of a 30% decline or extinction of local government nationwide is expected by 2040 as we face not only a decline in general and ageing populations but also the era of the demographic cliff. Thus, the government should continuously operate the "Special Committee on Regional Balanced Development" with a government-wide effort until 2030 to prevent disparities in the health conditions of local residents, which is the responsibility of the nation in terms of strengthening governance. To address the regional inequalities of rural and urban regions, it is necessary to re-adjust the basic subsidy and cost-sharing rates with local governments of current national subsidies based mainly on population scale, financial independence of local government, or distribution of healthcare resources and healthcare indices (showing high inequalities) overall.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.4802-4808
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• 2014

A plate-fin tube type heat exchanger has a lighter weight, approximately 30%, than the conventional circular-fin type condenser of household refrigerator. Because the low weight means low cost, it can have significant effects on the growth of related businesses if similar performance can be guaranteed. To check the possibility of the use of such a plate fin-tube condenser, experimental evaluations were performed in this study. Four different condensers including a conventional circular fin-tube condenser were used for the test. A well designed refrigerant supply system was used to supply similar conditions with a refrigerator, and the heat transfer rate and pressure drops of air side were measured precisely. As a result, the plate fin-tube type condensers showed a lower heat transfer rate of more than 13% than the conventional circular fin-tube type condenser, but the air side pressure drop was reduced and the heat transfer per unit weight was increased. Therefore, it shows the possibility of the use of a plate fin-tube type condenser after optimizing the air flow path and increasing the air flow to make a similar heat transfer rate.

• Microbiology and Biotechnology Letters
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• v.33 no.3
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• pp.169-177
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• 2005

This study focuses on investigating roles of microorganisms in decontamination of reed rhizosphere in Sunchon Bay, Korea, which is considered one of the marsh and mud environment severely affected by human activities such as agriculture and fisheries. In general, the bay is known to play the role of the buffering zone to reduce the sudden impact or change by environmental stresses. In our initial efforts to elucidate the microbial functions in decontamination process in reed rhizosphere, pure bacteria capable of degrading aromatic hydrocarbons were isolated from reed (Phragmites communis) rhizosphere of Sunchon bay by enrichment culture using either benzene, toluene, ethylbenzene, or xylene (BTEX) as a sole source of carbon and energy. Measurement of the rates of BTEX degradation and cell growth during the incubation in BTEX media under several temperature conditions demonstrated maximized degradation of BTEX at $37^{\circ}C$ in both strains. Both strains were also resistant to all the heavy metals and antibiotics tested in this study, as well as they grew well at $42^{\circ}C$. Identification of the isolates based on 16S rRNA gene sequences, and a variety of phenotypic and morphologic properties revealed that the two strains capable of BTEX catabolism were among Microbacterium sp., and Rhodococcus sp. with over $95{\%}$ confidence, designated Microbacterium sp. EMB-1 and Rhodococcus sp. EMB-2, respectively This result suggested that in the rhizosphere of reed, one of major salt marsh plants they might play an important roles in decontamination process of reed rhizosphere contaminated with petroleum such as BTEX.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.503-514
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• 1997

The morphogenesis of panicle and spikelet in paddy rice has been studied in high yielding Indica$\times$Japonica hybrid cultivar, Milyang 23 and a Japonica type cultivar, Koshihikari. Germinated seeds planted in $5000^{-1}$ a pots filled with submerged soil and cultured under natural conditions. The young panicle of main stem were continuously dissected and observered by Cryo-SEM from the panicle initiation stage until heading stage. Although the date of panicle differentiation and heading in Koshihikari earlier than those of Milyang 23. the sequence of panicle development in two cultivars begins when first bract primordium at opposite side of flag-leaf primordium differentiated, synchronously followed by growth of the primary branch primordia (PBPs) and secondary branch primordia (SBPs), spikelet primordia(SPs), glumes as lateral organs on rachilla and organs composing single floret, and successive sporogenesis in the young spikelets continue after the enclosure by lemma and palea. The PBPs are acropetally initiated from the base of the panicle primordium, and the SBPs alternately differentiate from the base of upper PBP which differentiate later than the lower PBP. Spikelet development starts at the top of upper side PBP of the young panicle and continue basipetally even though SBPs continue to develop at the lower primary branch. Each PBP, SBP and SP differentiate with differentiation bract or bract hair cell around the base of each their primordia. The observation could confirm that Milyang 23 has not only 2~3 more defferentiated PBPs, but also more SBPs and SPs especially from middle-lower primary branch, at end of their differentiation stages, as compared to those of Koshihikari.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.4
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• pp.71-82
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• 1979

Transparent vinyl-mulching cultivation plots heavy-fertilized (6-14-20 kg $10a^{-1}\; of\; N-P_2O_5-K_2O)$ in growing peanuts produced 315-344Kg $10a^{-1}$ of quality kernels through improvement of the low temperature conditions of underground environment during the early growing stage and kernel-filling stage. The yields of which were 82-99 percent higher than that of conventional cultivation plot. The vinyl-mulching cultivation techniques accelerated emergence, young seedling growth, flowering, pod bearing and kernel filling of peanuts in Korea.

• Journal of Animal Science and Technology
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• v.51 no.6
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• pp.459-470
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• 2009

The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.47-52
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• 2006

This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control>250 mM>500 mM treatments in order. The photosynthesis rate of cucumber at $30^{\circ}C$ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at $40^{\circ}C$ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 $mg{\cdot}L^{-1}\;CO_2$ supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of $F_v/F_m$ and $F_m/F_o$ of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at $40^{\circ}C$ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were $43^{\circ}C$ in the control, $44^{\circ}C$ in the 250 mM and $46^{\circ}C$ in the 500 mM according to analyze chlorophyll fluorescent $F_o$. The high temperature tolerance in cucumber increased approximately $3^{\circ}C$ by the hydrogen peroxide treatments under this experiment conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.3
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• pp.191-198
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• 2005

In order to optimize tissue culture conditions for genetic transformation of orchardgrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of 3 cultivars, 'Frode'. 'Roughrider' and 'Frontier' as explant tissues. Callus induction medium containing 3mg/L 2,4-D or 3mg/L dicamba each with 0.1mg/L BA was optimal fer embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The regeneration frequency from embryogenic callus among cultivars were descending order of Roughrider > Frode > Frontier. Supplementation of the regeneration media with 10mg/L $AgNO_3$ and 40mg/L cysteine enhanced frequency of plant regeneration. Efficient regeneration system established in this study will be useful fur molecular breeding of orchardgrass through genetic transformation.