Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators and Antioxidants on Callus Induction and Plant Regeneration from Seed Culture of Orchardgrass

오차드그래스의 종자배양에 있어서 식물생장조절물질과 항산화제가 캘러스유도와 식물체 재분화에 미치는 영향

  • Lee Ki-Won (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Sang-Hoon (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Dong-Gi (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo Hyun-Sook (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim Do-Hyun (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi Myung Suk (Division of Forest Science, Gyeongsang National University) ;
  • Kim Ki Young (National Livestock Research Institute) ;
  • Lee Hyoshin (Biotechnology Division, Korea Forest Research Institute) ;
  • Lee Byung-Hyun (Division of Applied Life Science, Gyeongsang National University)
  • 이기원 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 김도현 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 김기용 (농촌진흥청 축산연구소) ;
  • 이효신 (국립산림과학원) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Published : 2005.09.01
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Abstract

In order to optimize tissue culture conditions for genetic transformation of orchardgrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of 3 cultivars, 'Frode'. 'Roughrider' and 'Frontier' as explant tissues. Callus induction medium containing 3mg/L 2,4-D or 3mg/L dicamba each with 0.1mg/L BA was optimal fer embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The regeneration frequency from embryogenic callus among cultivars were descending order of Roughrider > Frode > Frontier. Supplementation of the regeneration media with 10mg/L $AgNO_3$ and 40mg/L cysteine enhanced frequency of plant regeneration. Efficient regeneration system established in this study will be useful fur molecular breeding of orchardgrass through genetic transformation.

오차드그래스의 최적 조직배양조건을 확립하기 위하여 3가지 품종의 성숙종자로부터 배발생 캘러스 유도 및 식물체 재분화에 미치는 배지첨가물질의 영향을 조사하였다. 성숙종자로 부터 배발생 캘러스 유도시 첨가되는 생장조절 물질로는 3mg/L 2,4-D 또는 dicamba와 0.1mg/L BA를 혼용첨가했을 때가 단용첨가구에 비해 높은 배발생 캘러스 유도효율을 나타내었다. 품종별 식물체 재분화 효율은 'Roughrider'가 가장 높게 나타났으며, 'Frode'가 중간정도, 'Frontier'가 가장 낮은 효율을 나타내었다. 식물체 재분화배지에 항산화물질로서 10mg/L의 $AgNO_3$와 40mg/L의 cysteine을 첨가해준 결과 식물체 재분화율이 증가되었다. 본 연구를 통하여 확립된 오차드그래스의 효율적인 조직배양체계는 유전자 형질전환을 통한 신품종 오차드그래스 개발에 유용하게 응용되어질 수 있을 것이다.

Keywords

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