Browse > Article
http://dx.doi.org/10.5187/JAST.2009.51.6.459

Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition  

Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Kim, Seong-Kon (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Park, Mi-Rim (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Park, Min-Ah (Dept. of Agricultural Science, Korea National Open University)
Jang, Eun-Joung (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Hong, Seung-Gu (Nutrition and Physiology Team, National Institute of Animal Science, R.D.A.)
Chang, Jong-Soo (Dept. of Agricultural Science, Korea National Open University)
Yoon, Du-Hak (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
Publication Information
Journal of Animal Science and Technology / v.51, no.6, 2009 , pp. 459-470 More about this Journal
Abstract
The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.
Keywords
Satellite cells; Depot; Proliferation; Hormone;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Spagnuolo, P. A., Bird, R. P. and Hoffman-Goetz, L. 2007. Effect of short-term dietary intake of bovine lactoferrin on intestinal lymphocyte apoptosis in healthy mice. Nutrition 23:812-817.   DOI   ScienceOn
2 Thompson, S. H., Boxhorn, L. K., Kong, W. Y. and Allen, R. E. 1989. Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I. Endocrinology. 124:2110-2121.   DOI   ScienceOn
3 Thompson, S. H., Boxhorn, L. K., Kong, W. Y. and Allen, R. E. 1989. Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulinlike growth factor I. Endocrinology 124:2110-2117.   DOI   ScienceOn
4 Tonge, R., Shaw, J., Middleton, B., Rowlinson, R., Rayner, S., Young, J., Pognan, F., Hawkins, E., Currie, I. and Davison, M. 2001. Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics. 1:377-396.   DOI   ScienceOn
5 Wang, S., Z, Ying., Y, Yun., Li, X., Guo-qiang, C., Yong-zong, Y. and Li-shun, W. 2008. Phosphorylation of b-actin by protein kinase C-delta in camptothecinanalog-induced leukemic cell apoptosis. Acta Pharmacol Sin. 29:135-142.   DOI   ScienceOn
6 Yongfeng Wei, Tolib B. Sanni, Peter X. F., Atadja, J. and Pili, R. 2007. Antitumor effect of the histone deacetylase inhibitor LAQ824 in combination with 13-cis-retinoic acid in human malignant melanoma. Mol. Cancer. Ther. 6:70-81.   DOI   ScienceOn
7 Abraham, M. R., Jahangir, A., Alekseev, A. E. and Terzic, A. 1999. Channelopathies of inwardly rectifying potassium channels. J. 13:1901-1910.   DOI
8 Allen, R. E. and Boxhorn, L. K. 1987. Inhibition of skeletal muscle satellite cell differentiation by transforming growth factor-$\beta$. J. Cell. Physiol. 133:567-573.   DOI
9 Murphy, C., McGurk, M., Pettigrew, J., Santinelli, A., Mazzucchelli, R., Johnston, G. P., Montironi, R. and Waugh, D. J. 2005. Nonapical and cytoplasmic expression of interleukin-8, CXCR1, and CXCR2 correlateswith cell proliferation and microvessel density in prostate cancer. Clin. Cancer Res. 11:4117.   DOI   ScienceOn
10 Moss, F. P. and Leblond, C. P. 1971. Satellite cells as the source of nuclei in muscles of growing rats. Anat. Rec. 170:421-435.   DOI   ScienceOn
11 Rabilloud, T. 1998. Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis, Electrophoresis 19:758-760.   DOI   ScienceOn
12 Sebestyen, A., Barnaa, G., Nagya, K., Janosi, J., Paku, S., Kohut, E., Berczi, L., Mihalik, R. and Kopper, L. 2005. Smad signal and TGFb induced apoptosis in human lymphoma cells. Cytokine. 30:228-235.   DOI   ScienceOn
13 Sinha-Hikim, I., Artaza, J., Woodhouse, L., Gonzalez-Cadavid, N., Singh, A. B. and Lee, M. I. 2002. Testosterone-induced increase in muscle size in healthy young men is associated with muscle fiber hypertrophy. Am. J. Physiol. Endocrinol. Metab. 283:E154-164.   DOI
14 Sinha-Hikim, I., Roth, S. M., Lee, M. I. and Bhasin, S. 2003. Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men. Am. J. Physiol. Endocrinol. Metab.285:E197-205   DOI
15 Kampa, M., Bakogeorgou, E., Hatzolgou, A., Damianaki, A., Martin, P. M. and Castanas, E. 1997. Opioid alkaloids and casomorphin peptides decrease the proliferation of prostatic cancer cell lines (LNCaP, PC3 and DU145) through a partial interaction with opioid receptors. Eur. J. Pharmacol. 335:255-65.   DOI   ScienceOn
16 Kubo, Y., Adelman, J. P., Clapham, D. E., Jan, L. Y., Karschin, A., Kurachi, Y., Lazdunski, M., Nichols, C. G., Seino, S. and Vandenberg, C. A. 2005. International Union of Pharmacology. LIV. Nomenclature and molecular relationships of inwardly rectifying potassium channels. Pharmacol. Rev. 57:509-526.   DOI   ScienceOn
17 Mampuru, L. J., Chen, S., Kalenik, J. L., Bradley, M. E. and Lee, T. 1996. Analysis of events associated withsSerum deprivation-induced apoptosis in C3H/Sol8 muscle satellite cells. Exp. Cell. Res. 226:372-380.   DOI   ScienceOn
18 Landry, F., Lombardo, C. R. and Smith, J. W. 2000. A method for application of samples to Matrix-Assisted Laser Desorption Ionization Time-of-Flight targets that enhances peptide detection. Anal. Biochem. 279:1-8.   DOI   ScienceOn
19 Lee, E. J., Choi, J., Hyun, J. H, Cho, K. H., Hwanf, I., Lee, H. J., Chang, J. and Chi, I. 2007. . Asian-Aust. J. Ani. Sci. 20: 501-510.   DOI
20 Lindquist, D. L. and de Alarcon, P. A. 1987. Charcoal-dextran treatment of fetal bovine serum removes an inhibitor of human CFU-megakaryocytes. Exp. Hematol. 15:234-238.
21 McCuscker, R. H. and Campion, D. R. 1986. Effect of growth-hormone secreting tumors on skeletal muscle cellularity in the rat. J. Endocrinol. 111:279-285.   DOI   ScienceOn
22 Mirua, S., Takeshita, T., Asao, H., Kimura, Y., Murata, K., Sasaki, Y., Hanai, J., Beppu, H., Tsukazaki, T., Wrana, J. L., Miyazono, K. and Sugamura, K. 2000. Hgs (Hrs), a FYVE Domain Protein, Is Involved in Smad Signaling through Cooperation with SARA. Mol. Cell. Biol. 20:9346-9355.   DOI
23 Hunt, D. W., Henricks, D. M., Skelley, G. C. and Grimes, L. M. 1991. Use of trenbolone acetate and estradiol in intact and castrate male cattle: Effect of growth, serum hormones, and carcass characteristics. J. Ani. Sci. 69:2452-2462.   DOI
24 Johnson, B. J., Anderson, P. T., Meiske, J. C. and Dayton, W. R. 1996. Effect of a combined trenbolone acetate and estradiol implant on steroid hormone levels, feedlot performance, carcass characteristics and carcass composition of feedlot steers. J. Anim. Sci. 74:363-71.   DOI
25 Goodman, A., Goode, B. L., Matsudaira, P. and Fink, G, R. 2003. The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol. Biol. Cell. 14: 2617-29.   DOI   ScienceOn
26 Johnson, B. J., Halstead, N., White, M. E., Hathaway, M. R. and Dayton, W. R. 1998. Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant. J. Anim. Sci. 76: 2779-86.   DOI
27 Kamanga-Sollo, E., Pampusch, M. S., Xi, G., White, M. E., Hathaway, M. R. and Dayton, W. R. 2004 IGF-I mRNA levels in bovine satellite cell cultures: effects of fusion and anabolic steroid treatment. J. Cell. Physiol. 201:181-189.   DOI   ScienceOn
28 Kamanga-Sollo, E., White, M. E., Hathaway, M. R., Chung, K. Y., Johnson, B. J. and Dayton, W. R. 2008. Roles of IGF-1 and the estrogen, androgen and IGF-1 receptors in estradiol-17$\beta$-and trenbolone acetate-stimulated proliferation of cultures bovine satellite cells. Domest. Anim. Endocrinol. 35: 88-97.   DOI   ScienceOn
29 Greene, E. A. and Allen RE. 1991. Growth factor regulation of bovine satellite cell growth in vitro. J. Ani. Sci. 69:146-152.   DOI
30 Halevy, O., Krispin, A., Leshem, Y., MCMurthy, J. P. and Yahav, S. 2001. Early-age heat exposure affects skeletal muscle satellite cell proliferation and differentiation in chicks. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 281: R302-R309.   DOI
31 Hansen-Smith, F. M., Picou, D. and Golden, M. N. H. 1979. Muscle satellite cells in malnourished and nutritionally rehabilitated children. Neurol. Sci. 41:207-221.   DOI   ScienceOn
32 Hayden, J. M., Williams, J. E. and Collier, R. J. 1993. Plasma growth hormone, insulin-like growth factor, insulin, and thyroid hormone association with body protein and fat accretion in steers undergoing compensatory gain after dietary energy restriction. J. Anim. Sci. 71:3327-3338.   DOI
33 Brenda, Y. T., Salumbides, C., Wang, David Z. Qian, Simon Williams, Burd, C. G. and Emr, S. D. 1998. Phosphatidylinositol (3)-phosphate signaling mediated by specific binding to ring FYVE finger domains. Mol. Cell. 2:157-162.   DOI   ScienceOn
34 Hellmann, U., Wemstedt, C., Gonez, J. and Heldin, C. H. 1995. Improvement of an “In-gel” digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal. Biochem. 224:451-455.   DOI   ScienceOn
35 Herschler, R. C., Olmsted, A. W., Edwards, A. J., Hale, T., Montgomery, R. L., Preston, S. J. and Sheldon, J. J. 1995. Production responses to various doses and ratio of estradiol benzoate and trenbolone acetate implants in steers and heifers. J. Ani. Sci. 69:2452-2462.
36 Hill, D. J., Crace, S. P., Nissley, Morell, D., Holder, A. T. and Milner. R. D. G. 1985. Fetal rat myoblasts release both rat somatomedin-C (SM-C)-in-like growth factor I QGF-I) and multiplication-stimulating activity in vitro: partial characterization and biological activity of myoblastderived SM-C/IGF-I. Endocrinology 117:2061.   DOI   ScienceOn
37 Byun, Y., Chen, F., Chang, R., Trivedi, M., Green, K. J. and Cryns, V. L. 2001. Caspase cleavage of vimentin disrupts intermediate filaments and promotes apoptosis, Cell Death Differ. 8:43-450.
38 Callebert, J., Esteve, J. M., Herve, P., Peoch, K., Tournois, C., Drouet, L., Launay, J. M. and Maroteaux, L. 2006. Evidence for a control of plasma serotonin levels by 5-hydroxytryptamine (2B) receptors in mice. J. Pharmacol. Exp. Ther. 317:724-731.   DOI   ScienceOn
39 Campion, D. R. 1984. The muscle satellite cell: a review. Int. Rev. Cytol. 87:225-251.   DOI
40 Cooper, R. N., Tajbakhsh, S., Mouly, V., Cossu, G., Buckingham, M. and Butler-Browne, G. S. 1999. In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle. J. Cell. Sci. 112:2895-2901.
41 Florini, J. R. and Magri, K. A. 1989. Effects of growth factors on myogenic differentiation. Am. J. Physiol. 256:701-711.   DOI
42 Dodson, M. V., McFarland, D. C., Grant, A. L., Doumit, M. E. and Velleman, S. G. 1996. Extrinsic regulation of domestic animal-derived satellite cells. Domest. Anim. Endocrinol. 13:107-26.   DOI   ScienceOn
43 Doumit, M. E. and Merkel, R. A. 1992. Conditions for the isolation and culture of porcine myogenic satellite cells. Tissue and cell. 24:253-262.   DOI   ScienceOn
44 Doumit, M. E., Cook, D. R. and Merkel, R. A. 1996 Testosterone up-regulates androgen receptors and decreases differentiation of porcine myogenic satellite cells in vitro. Endocrinology 137:1385-1394.   DOI   ScienceOn
45 Folkman, J. 2006. Antiangiogenesis in cancer therapy-endostatin and its mechanisms of action. Exp. Cell. Res. 312:594-607.   DOI   ScienceOn
46 Allen, R. E. and Rankin, L. L. 1990. Regulation of satellite cells during skeletal muscle growth and development. Proc. Soc. Exp. Biol. Med. 94:81-86.
47 Allen, R. E., Sheeha, S. M., Taylor, R. G., Kendall, T. L. and Rice, G. M. 1995. Hepatocyte growth factor activates quiescent skeletal muscle satellite cells in vitro. J. Cell. Physiol. 165: 307312.
48 Apple, J. K., Dikeman, M. E., Simms, D. D. and Kuhl, G. 1991. Effects of synthetic hormone implants, singularly or in combinations, on performance, carcass traits, and longissimus muscle palatability of Holstein steers. J. Ani. Sci. 69:4437-4448.   DOI
49 Azmitia, E. C. 2001. Modern views on an ancient chemical: Serotonin effects on cell proliferation, maturation, and apoptosis. Brain Res. Bull. 56:413-424.   DOI   ScienceOn
50 Bailey, S. R., Polan, J. L., Munoz, O. C., Agrawal, M. C., and Goswami, N. J. 2004. Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds. Cardiovasc. Radiat. Med. 5:119-124.   DOI   ScienceOn
51 Bhasin, S., Woodhouse, L., Casaburi, R., Singh, A. B., Bhasin, D. and Berman, N. 2001. Testosterone dose-response relationships in healthy young men. Am. J. Physiol. Endocrinol. Metab. 281:E1172-1181.   DOI
52 Blishchenko, E. Y., Sazonova, O. V., Kalinina, O. A., Yatskin, O. N.,. Philippova, M. M., Surovoy, A. Y., Karelin, A. A., and Ivanov, V. T. 2002. Family of hemorphins: co-relations between aminoacidsequences and effects in cell cultures, Peptides 23:903-910.   DOI   ScienceOn
53 Bock, J., Szabo, I., Jekle, A. and Gulbinsa, E. 2002. Actinomycin D-induced apoptosis involves the potassium channel Kv1.3. Biochem. Biophys. Res. Commun. 295:526-531.   DOI   ScienceOn
54 Braden, C., McFarland, Jerry Stewart, Jr., Hamza, A., Nordal, R., Davidson, D. J., Henkin, J. and Gladson., C. L. 2009. Plasminogen Kringle 5 induces apoptosis of brain microvessel endothelial cells: sensitization by radiation and requirement for GRP78 and LRP1. Cancer Res. 69:5537-5545.   DOI   ScienceOn