• Journal of Plant Biology
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• 제12권3호
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• pp.15-25
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• 1969

To investigate the radiation effect of P-32 on the soybean plants, the seeds treated with various levels of P-32 solution were sown and cultured in the pots. The growth of the plants and the contents of the macroelements were observed and the following results were obtained. 1) The linear growth of the plants at the early stage seemed to have been promoted by the low-level P-32 treatemnt. At the later growing stage, however, this difference among treatments were less conspicuous. 2) The plants of high level P-32 application showed some growth damaging symptom at the early growing stage. Later this damage was recorded as the time went on and these plants showed even better growth than the control. As a result at the late growing stage, they ensued highest growth. 3) The plants showed in general more growth at the low activity level than at the high-level at the early growing stage. At the late stage, however, the high-level activity promoted more growth than the low-level. 4) At the early growing stage P-32 treatment produced in general significantly more lower than control. At the later stage, however, this difference was not clearly seen. 5) The P-32 treatment seemed to have stimulated earlier florescence and this tendency was more clearly observable eapecially at the high activity level. 6) The weight of the air-dried seeds tended to be increased through P-32 treatment by 10-45%. This tendency was clearly observed especially at the low-level activity. 7) As for the contents of the various macroelements in the leaves, the nitrogen showed significantly larger contents at the middle level(S1) P-32 treatments. The phosphorous contents showed also highest at the middle levels activity and lower both at the high and low-activity levels. The potassium contents was proved, on the contrary, higher at the low-level activity and lower at the high-level. 8) The nitrogen contents in the stems was found significantly higher than control, except at the low-activity level. The phosphorous showed higher contents at the low-activity level and no significant difference at the high-activity level. As for the contents of potassium, calcium and magnesium, three seemed no significant difference among treatments. However, the magnesium showed somewhat higher content at the low-activity level, whereas the calcium was proved high than control. 9) The inorganic contents in the root showed that N and P in the P-32 treated plant were significantly higher than the control and the K-contents was, on the contrary, significantly higher at the control than the rest of the treatments. As for the calcium and magnesium there showed no difference among all treatments.

• Journal of Periodontal and Implant Science
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• 제26권2호
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• pp.414-428
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• 1996

The use of basic fibroblast growth factor which function as potent biologic mediators regulating numerous activities of wound healing has been suggested for the promotion of periodontal regeneration. The mitogenic effects of basic fibroblast growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'deoxy-uridine into DNA of the cells in a dose -dependent manner. The cells which were prepared were the primary cultured gingival fibroblasts and periodontal ligament cells from human the fourth or sixth subpassages were used in the experiments. The cells which were seeded DMEM contain 10% FBS. The added concentrations of basic fibroblast growth factor were 0.1, 1, 10, 50, $l00{\eta}g/ml$ and basic fibroblast growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10{\mu}l/200{\mu}l$ 5Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows. : The DNA synthetic activity of human gingival fibroblasts was increased dose dependently by basic fibroblast growth factor at 24 hours, 48 hours and 72 hours. The similar mitogenic effects were at the 24 and 48 hours of basic fibroblast growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells was increased dose dependently to $50{\eta}g/ml$ by basic fibroblast growth factor at 24, 48 and 72 hours, but the DNA synthetic activity decreased at $l00{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were at the 48 hours application of basic fibroblast growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 72 hours than at 24, 48 hours the application of basic fibroblast growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the basic fibroblast growth factor.In conclusion, basic fibroblast growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

• Journal of Plant Biology
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• 제11권1호
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• pp.1-6
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• 1968

The effects of the different temperatures on the amylase activity and growth rate of the rye seedling grown in the solutions containing radioactive sulfur- 35 were studied. The amylase activity of the coleoptiles obtained from the seedlings grown in the solutions of S-35, at 14$^{\circ}C$, appeared to be strongly stimulated in comparison to the control, but the culture temperatures of 22$^{\circ}C$ and 3$0^{\circ}C$ showed the decrease in the amylase activity. The amylase activity of the grains treated with the low intensity of the ratioactive material didn't show clear changes, at any culture temperatures, but the amylase activity of the grains treated with the high intensity of S-35, 50$\mu$c, showed definite decline at the elevated culture temperature, 3$0^{\circ}C$. Similar effects was also found in the growth of the seedlings. However, we would consider the effects of the radioactive materials on the acticity of the amylase and the growth of the seedlings are resulted from the accumulation of the much amount of the radioactive materials, and this accumulation rate depends upon actually the elevation of the culture temperature.

• Journal of Periodontal and Implant Science
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• 제26권4호
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• pp.841-858
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• 1996

Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.187-194
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• 2015

인간의 위장관은 매우 복잡한 장내균총으로 이루어져 있으며, 장내균총은 인간의 생리 기능에 있어 매우 중요한 역할을 담당하고 있다. 특히 유아기의 장내균총의 불균형은 면역연관 질병을 일으키는 요인이며 치료 목적으로 probiotics가 유용하게 활용되고 있다. Probiotics의 대표 균종은 L. plantarum으로서 영유아기에 병원균보다 가장 먼저 정착해야 면역 질환을 예방할 수 있다. 그러나 아직 Lactobacillus가 장의 우점종으로 정착하기 위한 환경적 요인과 항생물질분비 조절에 대한 이해는 부족한 실정이다.

• 디지털융복합연구
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• 제16권7호
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• pp.71-78
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• 2018

본 연구는 한국경제의 불공정 해소 방안으로 주목받고 있는 기업의 동반성장 활동이 기업의 경영성과에 미치는 영향을 실증적으로 분석함으로써 동반성장 활동이 기업 경영에 미치는 영향을 검증하고자 하였다. 연구기간은 2011년부터 2015년까지이며, 가설을 검증함에 있어 동반성장 활동기업의 표본구성의 특성을 고려하여 전체 상장기업을 대상으로 한 연구표본과 동반성장 활동기업만을 대상으로 한 연구표본으로 각각 구분하여 다중회귀분석을 통해 연구가설을 검증하였다. 분석결과, 기업의 동반성장 활동수준(W-W Index_N)과 경영성과(ROA, ROE, 매출액영업이익률)는 5% 유의수준에서 음(-)의 관련성을 나타냈다. 또한, 기업의 동반성장 활동수준(W-W Index_N)과 경영성과(매출액순이익률)는 10% 유의수준에서 음(-)의 관련성을 나타냈다. 이는 기업의 동반성장 활동이 기업의 비용 증가로 이어져 경영성과에는 부정적인 영향을 미치는 것으로 해석할 수 있다. 따라서 본 연구에 따르면 동반성장 활동의 촉진 및 장려를 위한 국가당국의 정책적 고려가 필요할 것으로 판단된다. 한편, 본 연구는 동반성장 활동의 초기 실증연구로, 향후 동반성장 활동이 더욱 활발해짐에 따라 이에 따른 추세변화 후속연구도 필요할 것으로 기대된다.

• 동아시아식생활학회지
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• 제12권3호
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• pp.241-248
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• 2002

The dried Prunus mume, an alkaline food abundant in organic acids (citric acid, malic acid and tartaric acid), has been largely used in both folklore remedies and Chinese herbal medicine for a long time. This study was performed to investigate the antimicrobial activity of the dried Prunus mume. The fractionation of the methanol extracts from Prunus mume was conducted using petroleum ether, chloroform, ethyl acetate and butanol, respectively. The antimicrobial activity of the Prunus mume extracts was then determined against food-borne pathogens using a paper disc method. The ethyl acetate extract showed the strongest antimicrobial activity against the eigth food-born pathogens used in this present study. Diaion HP 20 column chromatography was performed to remove some sugars that might inhibit the antimicrobial activity of Prunus mume. The strongest antimicrobial activity of ethyl acetate fraction of Prunus mume was shown against Staphylococus aureus. The growth inhibition curve was determined using ethyl acetate extracts of Prunus mume against Staphylococus aureus, which showed the growth inhibition up to 72 hours at 1,000 ppm concentration.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.414-421
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• 1999

Helicobacter pylori were found to be resistant to azide but sensitive to vanadate, suggesting that defect in the P-type ATPase activity rather than F-type ATPase would be lethal to cell survival or growth. To elucidate the relationship between this enzyme inhibition and H. pylori death, we determined the effect of omeprazole (OMP) plus vanadate on enzyme activity and cell growth. The minimum inhibitory concentration (MIC; ca. 0.8$\mu$mol/disk) of vanadate for H. pylori growth was lowered over l0-fold with the aid of OMP, whereby its inhibitory potential toward the P-type ATPase activity was diametrically increased. Alternatively, we found that this enzyme activity was essential for active transport in H. pylori. From these observations, we strongly suggest that the immediate cause of the growth inhibition of H. pylori cells with OMP and/or vanadate might be defective in the cell's active transport due to the lack of P-type ATPase activity. From the spectral data with circular dichroism (CD) spectroscopy, we found that activated OMP (OAS) at concentration below MIC did not disrupt helical structures of membrane proteins. Separately, we determined the cytopathic effect of OAS by SDS-PAGE, indicating the change in the production of cytoplasmic protein but not cell membrane.

• Mycobiology
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• 제30권3호
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• pp.175-179
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• 2002

A study on comparative efficacy and in vitro activity of fresh cow urine and cow dung for controlling Sclerotinia rot caused by Sclerotinia sclerotiorum of cucumber was carried out following mycelial growth inhibition test, treated and untreated sclerotia with these organic matters at different days of incubation. Results showed that cow urine suppressed more effectively the mycelial growth even after 5 days of incubation in comparison to cow dung. The highest inhibition 75.9% of mycelial growth was recorded in cow dung potato dextrose agar(CUPDA) after 3 days of incubation and least 22.7% was in cow dung potato dextrose agar(CUPDA) after same days of incubation. Mycelial growth from sclerotia of S. sclerotiorum was also influenced by PDA medium mixed with cow urine and cow dung. After 6 days of incubation in CUPDA mycelial growth was only 12.9 mm whereas in CDPDA and PDA the corresponding growth at the same time were 65.8 mm and 80.0 mm. Treated sclerotia of the selected fungus with cow urine had a very effective role on suppression of mycelial growth than that of untreated one. No mycelial growth was observed up to 4 days in treated sclerotia with cow urine. After 5 days only 0.9 mm mycelial growth was measured in treated sclerotia, while in case of untreated sclerotia the growth was 42.6 mm. Application of cow urine and cow dung on growing plants inoculated with the pathogen at different concentrations also proved their inhibitive effects.

• KSBB Journal
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• 제6권2호
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• pp.195-199
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• 1991

The effects or polyamines on growth, anthocyanin contents and $\beta$-glucan synthetase(GSII) activity in carrot hairy root were studied. Growth of hairy root was stimulated somewhat when each polyamine concentration was treated, especially addition of 1mM spermidine resulted in about 20% increase. On the whole, the axial diameter of hairy root was increased in response to increase in concentration of polyamine. On the other hand, GSII activity was stimulated in response to increase in concentration of polyamine, especially addition of 1mM spermine resulted in about 100% increase of activity. Therefore increased activity of GSII stimulated growth and thickness of hairy root. Anthocyanin contents were not affected by the polyammine.