• Title/Summary/Keyword: growth condition

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Mouse Melanoma Cell Migration is Dependent on Production of Reactive Oxygen Species under Normoxia Condition

  • Im, Yun-Sun;Ryu, Yun-Kyoung;Moon, Eun-Yi
    • Biomolecules & Therapeutics
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    • v.20 no.2
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    • pp.165-170
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    • 2012
  • Cell migration plays a role in many physiological and pathological processes. Reactive oxygen species (ROS) produced in mammalian cells influence intracellular signaling processes which in turn regulate various biological activities. Here, we investigated whether melanoma cell migration could be controlled by ROS production under normoxia condition. Cell migration was measured by wound healing assay after scratching confluent monolayer of B16F10 mouse melanoma cells. Cell migration was enhanced over 12 h after scratching cells. In addition, we found that ROS production was increased by scratching cells. ERK phosphorylation was also increased by scratching cells but it was decreased by the treatment with ROS scavengers, N-acetylcysteine (NAC). Tumor cell migration was inhibited by the treatment with PD98059, ERK inhibitor, NAC or DPI, well-known ROS scavengers. Tumor cell growth as judged by succinate dehydrogenase activity was inhibited by NAC treatment. When mice were intraperitoneally administered with NAC, the intracellular ROS production was reduced in peripheral blood mononuclear cells. In addition, B16F10 tumor growth was significantly inhibited by in vivo treatment with NAC. Collectively, these findings suggest that tumor cell migration and growth could be controlled by ROS production and its downstream signaling pathways, in vitro and in vivo.

Growth Conditions of Natural Monument Old Big Trees in Gyeongsangnamdo, Korea (경상남도 천연기념물 노거수의 생육환경 연구)

  • Kim, Hyo-Jeong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.14 no.5
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    • pp.103-112
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    • 2011
  • Old big tree transcends the simple meaning of trees as they are the natural monuments that embody the people's history and culture of this land. The Cultural Heritage Administration of Korea(CHA) defines and protects old big tree based not only on the size of the tree but also on its definitive cultural and natural factors such as value, implications, and originality. This research aims to identify and analyze the growth conditions, soil conditions and location character of 20 old big tree in Gyeongsangnamdo korea. The research examined the soundness of the arboreal form, the degree of damage on the bark, as well as the quantity of leafs levels to evaluate the overall condition of growth and development. Also, 9 elements such as soil texture, nitrogen and organic matter content, soil pH, phosphoric acid and EC were further analyzed The research analyzed in correlation of Growth condition and soil. Tree health related positivity that total nitrogen and organic matter. The result which analyzes location character, With natural monument old big trees raising a hand the area where is contiguous appeared with the fact that the farming village style where the rice field and the arable land of field etc. This research aimed at generating some foundational reference data for the analysis of the habitation and management conditions of natural monument old big tree within the Gyeongsangnamdo korea.

Effects of water levels and soil nutrients on the growth of Iris laevigata seedlings

  • Lee, Eun Hye;Lee, Bo Eun;Kim, Jae Geun
    • Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.43-49
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    • 2018
  • Iris laevigata is geographically restricted and legally protected in Korea. In this study, a mesocosm study was conducted to examine the effects of environmental conditions such as water levels and soil nutrient conditions on the growth and survival of I. laevigata seedlings. Complete submergence lowered the total number of leaves, biomass, and survival rates. A rise in soil nutrients increased overall seedling growth and increased tiller numbers via the promotion of asexual reproduction. Also, we found that the lowest measured values of seedlings are associated with the most stressful condition due to the interaction of low soil nutrients and high water levels. I. laevigata seedlings, however, are distributed in low-nutrient habitats such as floating mat, even though they do not grow well under these conditions. This study suggests that I. laevigata does not prefer low-nutrient condition but choose another benefit such as low competition. Also, the water level must be lower than the seedling height for effective growth and management of I. laevigata.

Effect of surface roughness on the quality of silicon epitaxial film grown after UV-irradiated gas phase cleaning

  • Kwon, Sung-Ku;Kim, Du-Hyun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.5
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    • pp.504-509
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    • 1999
  • In-situ cleaning and subsequent silicon epitaxial film growth were performed in a load-locked reactor equipped with Hg-grid UV lamp and PBN heater to obtain the smooth and contaminant-free underlying surface and develop low-temperature epitaxial film growth process. The removals of organic and native oxide were investigated using UV-excited $O_2$ and $NF_{3}/H_{2}$, and the effect of the surface condition was examined on the quality of silicon epitaxial film grown at temperature as low as $750^{\circ}C$. UV-excited gas phase cleaning was found to be effective in removing the organic and native oxide successfully providing a smooth surface with RMS roughness of 0.5$\AA$ at optimal condition. Crystalline quality of epitaxial film was determined by smoothness of cleaned surface and the presence of native oxide and impurity. Crystalline defects such as dislocation loops or voids due to the surface roughness were observed by XTEM.

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The growth YMnO$_3$ single crystals using a floating zone method (부유대용융법에 의한 YMnO$_3$단결정 성장)

  • 권달회;강승구;김응수;김유택;심광보
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.4
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    • pp.279-285
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    • 2000
  • High quality crystals of $YMnO_3$, which is interested in non-volatile memory device application, were grown by the floating zone method. Optimum condition for powder synthesis was established to be $1200^{\circ}C$ for 10 hrs and optimum condition for sintering of $YMnO_3$feed-rod was established to be $1500^{\circ}C$ for 10hrs respectively. It was found from non-seeded growth experiment that $YMnO_3$crystal was grown preferentially to the [1010] orientation. The $YMnO_3$single crystal, which was grown to the direction of perpendicular to C-axis, was typically 5mm in diameter, 50 mm in length and showed dark-blue color.

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Growth Reaction of Some Ground Cover Plant in Korean Native Greening according to Shading Levels (몇 가지 자생 지피식물의 차광에 따른 생장반응)

  • Kim, Gui Soon;Lee, Jeong Sik
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.75-80
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    • 2009
  • The objective of this research was to select the native shade tolerance plant which grows well from landscape objective of the urban. Seven species native ground cover plants were evaluated on the growth and chloropyll under 0, 40, 60, 90% shading treatments. Hosta longipes was showed the quite good growth from 60% level of natural light. The plant growth of Liriope platyphylla was good within $144.2{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ intensity which is 90% shade level of natural light. But, Dryiopteris crassirhizoma was judged with shade tolerance plant which grow quite well from shading condition level of 60% degree. Carex siderosticea was showed the highest plant length and plant width in 40% shading of natural light more than at conditon too much shading. Hosta longipes, Liriope platyphylla, Dryiopteris crassirhizoma, and Carex siderosticea were as shade tolerance plant with increases chlorophyll content well to overcome a light restrictive condition and is adapted to seem with the result.

A classical two sector disequilibrium model of distribution and growth cycles with no long-period equilibrium (고전학파 2부문 불균형동학 모형)

  • Lee, Sangheon
    • 사회경제평론
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    • no.38
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    • pp.51-83
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    • 2012
  • Consider an n goods production economy. Assume the equilibrium condition of Sraffa's price system, a balanced growth condition and the goods market clearing conditions. If both equations are given to determine a real wage rate and investment, the economic system is over-determined. It suggests that there exists no long-period equilibrium to satisfy both labor market and goods market conditions. This paper interprets this situation of over-determinacy as a disequilibrium state, and attempts to solve it through disequilibrium dynamics. It constructs a model of accumulation and real wage rates consistent with Lotka-Volterra system, and shows that the overall growth path fluctuates endogenously around a resting point of long-period disequilibrium.

Temperature Effect on Seed Germination and Seedling Growth of Medicinal Umbelliferae Plants

  • Chio, Seong-Kyu;Chon, Sang-Uk
    • Plant Resources
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    • v.3 no.1
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    • pp.66-70
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    • 2000
  • Umbelliferae medicinal crops have low germination under natural condition. Special objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on their germination and seedling growth in Umbelliferae plants, using them as oriental medicine materials. Fruits of Umbelliferae are bi-partite type and form 2 seeds. Their oil canals and funiculus or carpaphore has apparently specific shape. Seed shape of Buplerum falcatum, Ligusticum acutilobum and Foeniculum vulgare are oblong, Ledeboutiella seseloides, Angelica dahurica, Angelica gigas elliptic, and Ostericum koreanum ovate. Color of seed coats varied from yellow to dark brown. Seed size was 3.2 to 6.9 mm in length, 1.7 to 4.9 mm in width. One thousand-seed weight of Buplerum falcatum was lowest(at 1,660 mg) and Ledebouriella seseloides and Angelica gigas were relatively highest(at 3,970 mg) having larger size than any others. Optimum temperatures for seed germination and seedling growth ranged from 20 to $25^{\circ}C$ and especially temperature at $25^{\circ}C$ improved seed germinations of Ledebouriella seseloides, Angelica dahurica and Ligusticum acutilobum regardless of light condition.

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Isolation of Bacillus sp. SHL-3 from the Dry Soil and Evaluation of Plant Growth Promoting Ability

  • Hong, Sun Hwa;Kim, Ji Seul;Sim, Jun Gyu;Lee, Eun Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.1
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    • pp.36-43
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    • 2015
  • Excess use of chemical fertilizer causes soil acidification and accumulation of salt, and thus might bring to desertification of soil. To overcome this problem, it needs limited usage of chemical fertilizer and increased usage of natural fertilizer as an alternative. In this study, dry soil-borne Bacillus sp. SHL-3, which was isolated from arid and barren soil, with plant growth promoting activity was isolated for identification and to determine optimal culture condition. A bacterial strain SHL-3 had the IAA productivity ($5.16{\pm}0.10mg\;L^{-1}$), ACC deaminase activity ($0.36{\pm}0.09$ at 51 hours) and siderophore synthesis. It was identified as genus Bacillus sp.. Also, optimal culture condition of SHL-3 were $20^{\circ}C$ and pH 7 in LB medium. Bacillus sp. SHL-3 had up to 4% salt tolerance in the medium. We evaluated the plant growth promotion ability of SHL-3 using yam (Dioscorea japonica Thunb.). As a result, Bacillus sp. SHL-3 was effective on the increase of the shoot length (202.4% increase for 91 days). These results indicate that Bacillus sp. SHL-3 can serve as a promising microbial resource for the biofertilizers of soil.

Studies on the Growth Characteristics of the Trees in Parking Lot in the Apartment Complex (아파트 단지 지하주차장 상부에 식재된 교목의 생장 특성에 관한 연구)

  • Jang, Byoung-Kwan;Yun, Ju-Cheul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.24 no.1
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    • pp.111-119
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    • 2021
  • This study was conducted to investigate the growth characteristics of artificially planted trees on top of the underground parking lots. The trees were planted 17 years ago and are now in good condition. The survey results are as follows. The planting depth of the trees was 0.9 - 1.3m. The horizontal roots of the trees were distributed in the depth of 0.2m ~ 0.6m, Growth condition was also considered to be good. Also, in the case of the straight root of the trees, the pine tree(Pinus densiflora) grew near the bottom but tree root was very thin, and the Japanese hackberry tree(Celtis sinensis) and the zelkova tree(Zelkova serrata) were seemed to bend at the bottom and to grow by changing direction. When trees were planted on artificial ground, the roots grew well horizontally, and the forces of growing vertically were much weakened. because the plants were planted in earth ball state. As a result, it was considered that the roots would hardly penetrate the bottom-pressing concrete floor.