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A STUDY ON SPECIFIC ACTIVITIES OF ENZYMES IN 7, 12-DIMETHYLBENZ(A)ANTHRACENE(DMBA)-INDUCED RAT SUBMAXILLARY GLAND CARCINOGENESIS (백서의 7, 12-Dimethylbenz(a)anthracene 유도 악하선 종양발암과정에서의 효소 특이활성도에 대한 연구)

  • Shim, Hyun-Goo
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.12 no.1
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    • pp.27-40
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    • 1990
  • In recent years, tissue antigens and enzymes that will serve as phenotypic markers for malignant cells are becoming increasingly important as diagnostic aids. This study was undertaken to investigate the specific activities of these enzymes in DMBA-induced rat submaxillary gland carcinogenesis. One hundred and twenty Sprague-Dawley rats about 100 gms of body weight were used. In experimental group, DMBA pellet (5mg) was implanted into right submaxillary gland and sham operation was performed into left gland to serve as control. The animals were sacrificed every three weeks up to 15 weeks. Submaxillary glands were excised on both sides and enzyme assays for ${\gamma}-glutamyl$ transpeptidase (GGT), 5'-Nucleotidase, Ornithine decarboxylase(ODC) and Acetyl-Co A carboxylase were carried out biochemically. The obtained results were as follows ; 1. In control group, there was no significant weight change of submaxillary gland, while experimental group, weight was increased remarkably about 7-fold at 15th week since DMBA implantation. 2. In control group, there was no change in specific activities of enzymes during the experimental period. 3. GGT activity was rapidly increased reaching a peak of 1.766${\pm}$0.082units/mg of DNA, 8-fold greater than that of onset. 4. 5'-Nucleotidase activity was increased reaching a peak of $362.1{\pm}53.2{\mu}moles/mg$ of DNA at 9th week. 5. ODC activity was rapidly increased, reaching a peak of 26.2${\pm}$4.8nmoles/mg of DNA at 9th week and quickly returned to that of control at 15th week. 6. Acetyl-Co A carboxylase activity was rapidly increased earlier than other enzymes, reaching a peak of 0.178${\pm}$0.013units/mg of DNA at 6th week and quickly declined to the control level at 15th week.

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The Effects of the Emotion Regulation Strategy to the Disgust Stimulus on Facial Expression and Emotional Experience (혐오자극에 대한 정서조절전략이 얼굴표정 및 정서경험에 미치는 영향)

  • Jang, Sung-Lee;Lee, Jang-Han
    • Korean Journal of Health Psychology
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    • v.15 no.3
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    • pp.483-498
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    • 2010
  • This study is to examine the effects of emotion regulation strategies in facial expressions and emotional experiences, based on the facial expressions of groups, using antecedent- and response- focused regulation. 50 female undergraduate students were instructed to use different emotion regulation strategies during the viewing of a disgust inducing film. While watching, their facial expressions and emotional experiences were measured. As a result, participants showed the highest frequency of action units related to disgust in the EG(expression group), and they reported in the following order of DG(expressive dissonance group), CG(cognitive reappraisal group), and SG(expressive suppression group). Also, the upper region of the face reflected real emotions. In this region, the frequency of action units related to disgust were lower in the CG than in the EG or DG. The results of the PANAS indicated the largest decrease of positive emotions reported in the DG, but an increase of positive emotions reported in the CG. This study suggests that cognitive reappraisal to an event is a more functional emotion regulation strategy compared to other strategies related to facial expression and emotional experience that affect emotion regulation strategies.

The Effects of a Group Art Therapy on Job Stress and Burnout among Clinical Nurses in Oncology Units (집단미술치료가 암병동 간호사의 직무스트레스와 소진에 미치는 효과)

  • Jang, Ok Jeom;Ryu, Ui Jeong;Song, Hyun Jeong
    • Journal of Korean Clinical Nursing Research
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    • v.21 no.3
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    • pp.366-376
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    • 2015
  • Purpose: The purpose of this study was to evaluate the effects of a group art therapy on decreasing job stress and burnout of clinical oncology nurses. Methods: The study was conducted using a non-equivalent control group, non-synchronized design with repeated measures. A total of 29 clinical oncology nurses participated in the study; 15 were assigned in the experimental group, and 14 in the control group. Data were collected from May to July, 2014. The study questionnaires were administered in three different time points (pretest, 8th week, and 12th week). Data were analyzed using $x^2-test$, Fisher's exact test, t-test, paired t-test, and repeated measures ANOVA. Results: The first hypothesis "the experimental group with group art therapy will have lower job stress than that of the control group" was support (F=7.88, p=.003). The second hypothesis, "the experimental group will have lower burnout than that of the control group" was not statistically supported (F=1.80, p=.188). Conclusion: The findings of the study indicated that the group art therapy for oncology nurses may positively influence in decreasing job stress, which open a venue for developing a nursing intervention with group art therapy for clinical nurses in future.

Effectiveness of Simple Trauma Team Activation Criteria on Prognosis of Severe Trauma Patients (단순 외상팀 활성화 조건이 중증 외상 환자의 치료 결과에 미치는 영향)

  • Lee, Dong Keon;Lee, Kang Hyun;Cha, Kyoung Chul;Park, Kyoung Hye;Choi, Han Joo;Kim, Hyun;Hwang, Sung Oh
    • Journal of Trauma and Injury
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    • v.22 no.1
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    • pp.71-76
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    • 2009
  • Purpose: The goal of this study was to compare the outcome of the after trauma team (AfterTT) group to the before trauma team (BeforeTT) group. Methods: All trauma patients who visited to emergency room (ER) between July 1, 2006 and February 29,2008 based on trauma registry, with systolic blood pressure (SBP) < 90 mmHg or GCS < 9 were included in this study. We compared the amount of packed RBC transfusion, the ER stay time, the ER visit to CT evaluation time, the ER visit to operation time, the length of ICU stay, the length of hospital admission and the survival discharge rate between the AfterTT group and the BeforeTT group. Patients with brain injuries had little chance of survival. Burn patients, who visited the ER 24 hours after injury and patients who were dead on arrival (DOA) were excluded from this study. Results: Total of 93 patients were included in this study: 42 in the AfterTT group and 51 in the BeforeTT group. The AfterTT group and the Before TT group showed no differences in Revised Trauma Score (RTS) and mean age. The amount of packed RBC transfusion was lower in the AfterTT group, but no statistically significant difference was noted (AfterTT 11${\pm}$11units, BeforeTT 16${\pm}$15units, p=0.136). The ER visit to operation time was shorter in the AfterTT group, but there were no statistically significant difference between the groups (AfterTT 251${\pm}$223 minutes, BeforeTT 486${\pm}$460 minutes, p=0.082). The length of ICU stay was shorter in the AfterTT group, but the difference was not statistically significant (AfterTT 11${\pm}$12 days, Before TT 15${\pm}$30 days, p=0.438). The length of Hospital admission was shorter in the AfterTT group (AfterTT 43${\pm}$37 days, BeforeTT 68${\pm}$70 days, p=0.032), but this difference was not statistically significant. Conclusion: Simple Trauma team activation criteria decreased the amount of packed RBC transfusion and the hospital admission duration. Hemodynamic instability (SBP < 90 mmHg) and decreased mental state (GCS<9) are good indices for activating the trauma team.

A study on the Residential Satisfaction and Demands for the Comprehensive Apartment Improvement Planning (공동주택 거주자의 주거환경 만족도 및 개선요구를 통한 공동주택 장기수선 계획 연구)

  • Yoon Chung-Sook;Kim Soo-Jeong;Shin Soo-Young;Kim Suk-Kyung;Abrams Robin F.
    • Journal of the Korean housing association
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    • v.17 no.4
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    • pp.37-46
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    • 2006
  • The primary purpose of this study is to provide the managerial guidelines for the comprehensive apartment improvement planning. This plan will consider the time-serial apartment management plan. Through a questionnaire survey, residential satisfactions and demands on apartment units, apartment building and site amenities were investigated. Based on the statistical analysis, residents' demands were assessed. The resident groups were categorized into the three groups considering the apartments' life span where they were living. The results from the statistical analysis were finally compared with the long-term apartment management plan demonstrated in the Housing Code of the Ministry of Construction and Transportation. The major findings of this study were as follows. First, residential satisfaction on the equipments in apartment units was higher than that on the unit plans. Residents' satisfaction on the communal facilities in apartment sites was lower than that on the other factors. Thus, apartment unit plans and communal facilities in sites need to be improved. Second, though we had the three residents' groups, for the results of the residential satisfaction, the groups were divided into two groups: 'less than 10 years group'and 'over than 10 years group'. Considering the residents' demands for the apartment improvement according to the life span of apartment complexes, the habitability factor was demanded by 'the less than 10 years group' and the safety factor by 'the over than 10 years group'. Compared the residents' demands for apartment improvements with the long-term apartment management plan demonstrated in the Housing Code, the improvement cycles demanded by residents were shorter than those in the code. Thus, the management plan in the code should be reconsidered.

A STUDY ON THE SHAR BOND STRENGTH OF LIGHT-CURED GLASS IONOMER CEMENTS TO DENTIN ACCORDING TO CURING TIME INDUCED BY ARGON LASER (아르곤 레이저의 조사시간에 따른 광중합형 글래스 아이오노머 시멘트의 상아질에 대한 전단결합강도에 관한 연구)

  • Cho, Young-Lin;Hwang, Ho-Keel;Cho, Young-Gon;Oh, Haeng-Jin
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.267-277
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    • 1997
  • The purpose of this study was to evaluate the shear bond strength of light-cured glass ionomer cements to dentin surface according to curing time induced by argon laser. In this study, 160 extracted human molars with sound crown were used. The dentin surface of these teeth were exposed with high speed diamond bur under water spray and polished with 120, 320, 400, 800, 1200 grits sand paper. 160 extracted human molars were randomly assigned into four groups (control, experimental 1, 2 & 3) with 40 teeth each. Control group used a visible light curing unit, XL 1000(3M Co., U.S.A) and experimental groups used argon laser($SPECTRUM^{TM}$). And then each group subdivided into two groups (A, B) according to filling materials. Subdivided A group used Fuji II LC(GC Co., Japan), B group used Vitremer(3M Co., U.S.A). The curing units and curing time of each group were as follows : Control group : visible light, 40 seconds Experimental group 1 Experimental group 2 Experimental group 3 : argon laser, 10 seconds : argon laser, 20 seconds : argon laser, 30 seconds The glass ionomer cements were bonded to dentin surface of each specimen. The specimens were stored in 100% relative humidity at 37"c for 7days. And then, the shear bond strength were measured by universal testing machine(Shimatzu Co. Japan) at crosshead speed of 5mm/min and 100kg in full scale and analyzed statistically. The following results were obtained : 1. Experimental group 2-A showed the highest shear bond strength with $9.87{\pm}1.24$ kgf and control group B showed the lowest shear bond strength with $4.08{\pm}0.78$ kgf(P<0.01). 2. The Fuji II LC showed higher shear bond strength with $9.49{\pm}1.24$ kgf than that of the Vitremer with $4.23{\pm}1.24$ kgf. There was significant difference between Fuji II LC and Vitremer(p<0.01). 3. There was no significant differences among experimental groups according to curing time induced by argon laser. 4. There was no significant differences between control group and experimental groups according to curing units.

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Diagnostic classification and clinical aspects of floppy infants in the neonatal and pediatric intensive care units (신생아 및 소아 중환자실에 입원한 늘어지는 영아(floppy infant)의 진단적 분류 및 임상적 고찰)

  • Kim, Eun Sun;Jung, Kyung Eun;Kim, Sang Duk;Kim, Eo Kyung;Chae, Jong Hee;Kim, Han Suk;Park, June Dong;Kim, Ki Joong;Kim, Beyong Il;Hwang, Yong Seung;Choi Jung-Hwan
    • Clinical and Experimental Pediatrics
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    • v.49 no.11
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    • pp.1158-1166
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    • 2006
  • Purpose : The purpose of this study is to make a diagnostic classification and discuss a diagnostic strategy of floppy infants by investigating clinical, neurological, electrophysiological, and genetic analysis of infants admitted to intensive care units with the complaint of hypotonia. Methods : A retrospective study was performed from Jan. 1993 to Dec. 2005 in neonatal and pediatric intensive care units of Seoul National University Children's Hospital. Clinical features and all tests related to hypotonia were investigated. Results : There were 21 cases of floppy infants admitted to intensive care units. Final diagnosis was classified as centra (7 cases[33.3 percent]), peripheral (11 cases [52.4 percent]), and unspecified (3 cases [14.3 percent]). Among the central group, three patients were diagnosed as hypoxic ischemic encephalopathy, two patients as Prader-Willi syndrome, one patient as chromosomal disorder, and one patient as transient hypotonia. Among the peripheral group, four patients were diagnosed as myotubular myopathy, three patients as SMA type 1, two patients as congenital myotonic dystrophy, one patient as congenital muscular dystrophy, and one as unspecified motor-neuron disease. Motor power was above grade 3 on average, and deep tendon reflex was brisk in the central group. Among investigations, electromyography showed 66 percent sensitivity in the peripheral group, and muscle biopsy was all diagnostic in the peripheral group. Brain image was diagnostic in the central group, and Prader-Willi FISH or karyotyping was helpful in diagnosis in central group. Morbidity and mortality was more severe in the peripheral group Conclusion : Classification of diagnosis by clinical characteristics in this study, and application of investigations step by step, may provide an effective diagnostic strategy.

Ecological Studies on the Vegetational Characteristics of the Abies koreana Forest (구상나무림(林)의 군락생태학적 연구)

  • Lee, Yoon Won;Hong, Sung Cheon
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.247-257
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    • 1995
  • This research carried out forest community classification on the basis of the methods of ZM school, in order to furnish useful information needed for forest management, and for afforestation and preservations of Abies koreana forest which was Korean native species, and the research results could be summarized as follows. Abies koreana forest was divided into 10 vegetation units ; This forest was divided into Abies koreana-Quercus mongolica community and Abies koreana-Sasa quelpartensis community. Abies koreana-Quercus mongolica community was divided into Tripterygium regelii group, Patrinia saniculaefolia group, and Typical group, and Tripterygium regelii group seas divided into Typical subgroup. Pimpinella brachycarpa subgroup, and Hemirocallis fulva subgroup. Abies koreana-Sasa quelpartensis community was divided into Berberis koreana group, Trachelospermum asiaticum var. intermedium group, and Typical group. Berberis koreana group was divided into Typical subgroup and Hepatica asiatica subgroup, and Tranchelospermum asiaticum var. intermedium group was divided into Hepatica asiatica subgroup and Typical subgroup. According to the results of the analysis by coincidence method, Abies koreana-Quercus mongolica community represented in Mt. Chiri, Mt. Deokyu and Mt. Kaya which were located in the Sobaek mountains, Abies koreana-Sasa quelpartensis community represented in Mt. Hanla. Therefore it was thought that Abies koreana forest was classified by geographical position, and vegetation units of Abies koreana forest tended to be classified by the altitude and topography. As for DBH and height of Abies koreana, mean DBH and height of Abies koreana-Quercus mongolica community were each 28.4cm, 10.6m that were larger than mean DBH and mean height(each 23.6cm, 6.3m) of Sasa quelpartensis community. Among Abies koreana-Quercus mongolica community, Patrinia saniculae-folia group had the smallest mean DBH and mean height(20cm, 5m), and among Abies koreana-Sasa quelpartensis community, Typical group had the smallest mean DBH and mean height(4cm, 3m).

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A Study on the Teaching Effect Based on the Learning Hierarchy in the "Life Continuity" (생명의 연속성 개념에서 학습위계에 따른 수업효과에 관한 연구)

  • Kim, Young-Shin;Chung, Wan-Ho
    • Journal of The Korean Association For Science Education
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    • v.15 no.2
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    • pp.233-240
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    • 1995
  • The purpose of the study is to certify the validity and effectiveness of the learning hierarchy and to define the effective teaching order in life continuity. To achieve this purpose, two experimental groups which were instructed varying the sequence of the instructional units. Teaching order based on the learning hierarchy was given to experimental group and descriptive order of current text was given to control group. The findings of the study are as follows: 1. Learning Hierarchy before learning had differ with order of the textbook, but hierarchy of both group has showed similar to the sequence of textbook, after learning. 2. The effect of learning hierarchy represented no significant different between control and experimental group.

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Characteristics of Interface Response of Optical Functionalized Dendrimer (광기능성 덴드리머의 계면반응 특성)

  • Son, J.H.;Shin, H.K.;Kim, B.S.;Kwon, Y.S.
    • Proceedings of the KIEE Conference
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    • 2001.11a
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    • pp.121-123
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    • 2001
  • We synthesized dendrimers containing light switchable units such as azobenzene group at the air/water interface. Their chemical structure was verified by using NMR study and UV spectroscopy. We firstly investigated the monolayer behavior and by light irradiation using $\pi$-A isotherm at the air/water interface. As a result, the monolayer of dendrimer with azobenzene group showed the reversible photo-switching behavior by the isomerization of azobenzene group in their periphery. This suggests that optical behavior and morphological change are affected by the functional group and the symmetric chain.

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