• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.161-167
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• 2010

Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with ElS. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50~9.67% and 8.50~9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29~30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.

• Korean Institute of Interior Design Journal
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• v.16 no.1 s.60
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• pp.126-134
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• 2007

This study researches the effectiveness of virtual experiences as an evaluation method in design. For the study, I researched the result on the spatial recognition before, abstracted visual objects and factors in recognition process and made up 43 inquiries for the evaluation through the experts group. Besides, set the analytical code between recognition factor and spatial constituents, made analysis the result from it. From April 1st to June 30th in 2004, the virtual model of educational place, college main building and city library was established as a subject of the research and studied the result. The methods that are used for the study are frequent analysis, T-test, reliability analysis, factor analysis etc. and SPSSWIN Package is used for the statistic analysis. There were two times of analysis on the evaluation to analyze the effect of virtual models on the web basis, one group evaluated the virtual building same as real and the other group evaluated the building site. Then analyzed the differences and correlation between the two. In result, college building, the first subject of study, and the second city library shows that made little differences between on the spot evaluation and virtual evaluation. Therefore, it is considered that the design evaluation with virtual model on the web basis has a vantage. But some problems founded that solved between preceding and present research through the feedback. It is as follows, 1. detection of problems that insufficience validity in the first survey. 2. found some not-validity inquiries. 3. detection of necessities that qualitative improvement and technical repletion in virtual environment.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2004.11a
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• pp.371-374
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• 2004

Induction motors are a critical component of many industrial processes and are frequently integrated in commercially available equipment. Safety, reliability, efficiency, and performance are some of the major concerns of induction motor applications. Preventive maintenance of induction motors has been a topic great interest to industry because of their wide range application of industry. Since the use of mechanical sensors, such as vibration probes, strain gauges, and accelerometers is often impractical, the motor current signature analysis (MACA) techniques have gained murk popularity as diagnostic tool. Fault tolerant control (FTC) strives to make the system stable and retain acceptable performance under the system faults. All present FTC method can be classified into two groups. The first group is based on fault detection and diagnostics (FDD). The second group is independent of FDD and includes methods such as integrity control, reliable stabilization and simultaneous stabilization. This paper presents the fundamental FDD-based FTC methods, which are capable of on-line detection and diagnose of the induction motors. Therefore, our group has developed the embedded distributed fault tolerant and fault diagnosis system for industrial motor. This paper presents its architecture. These mechanisms are based on two 32-bit DSPs and each TMS320F2407 DSP module is checking stator current, voltage, temperatures, vibration and speed of the motor. The DSPs share information from each sensor or DSP through DPRAM with hardware implemented semaphore. And it communicates the motor status through field bus (CAN, RS485). From the designed system, we get primitive sensors data for the case of normal condition and two abnormal conditions of 3 phase induction motor control system is implemented. This paper is the first step to drive multi-motors with serial communication which can satisfy the real time operation using CAN protocol.

• Clinical and Experimental Pediatrics
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• v.61 no.2
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• pp.43-48
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• 2018

Purpose: Human adenovirus infection mimics Kawasaki disease (KD) but can be detected in KD patients. The aim of this study was to determine the clinical differences between KD with adenovirus infection and only adenoviral infection and to identify biomarkers for prediction of adenovirus-positive KD from isolated adenoviral infection. Methods: A total of 147 patients with isolated adenovirus were identified by quantitative polymerase chain reaction. In addition, 11 patients having KD with adenovirus, who were treated with intravenous immunoglobulin therapy during the acute phase of KD were also evaluated. Results: Compared with the adenoviral infection group, the KD with adenovirus group was significantly associated with frequent lip and tongue changes, skin rash and changes in the extremities. In the laboratory parameters, higher C-reactive protein (CRP) level and presence of hypoalbuminemia and sterile pyuria were significantly associated with the KD group. In the multivariate analysis, lip and tongue changes (odds ratio [OR], 1.416; 95% confidence interval [CI], 1.151-1.741; P=0.001), high CRP level (OR, 1.039; 95% CI 1.743-1.454; P= 0.021) and sterile pyuria (OR 1.052; 95% CI 0.861-1.286; P=0.041) were the significant predictive factors of KD. In addition, the cutoff CRP level related to KD with adenoviral detection was 56 mg/L, with a sensitivity of 81.8% and a specificity of 75.9%. Conclusion: Lip and tongue changes, higher serum CRP level and sterile pyuria were significantly correlated with adenovirus-positive KD.

• Proceedings of KOSOMES biannual meeting
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• 2006.05a
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• pp.47-52
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• 2006

The cultural grounds of laver has been surveyed using SPOT-5 satellite images to calculate the facilities of laver cultivation area in the coastal waters of Korea 10m resolution multispectral images of SPOT-5 are adopted for the south area of Daebu Island, Hwaseong city to develop an automatic detection approach of laver nets that consists of the following: band difference technique, canny edge detector and morphological analysis. The satellite-based facilities number was relatively high as compared with the licensed number in 2005, 676,749 chaek and 572,745 chaek(柵, unit of measure for laver farm), respectively. The data could be applied to achieve a good harvest for laver seaweed growers and to control its national production keeping a stable market price for the government body.

• The Korea Journal of Herbology
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• v.30 no.4
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• pp.11-20
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• 2015

Objectives : Ongyeong-tang (OGT) is a traditional herbal formula used to cure gynaecological disorders. OGT consists of 12 herbal medicines containing various bioactive components. Therefore, the development of suitable analytical method for the marker compounds is necessary for the quality control of OGT. Methods : Determination of the 18 marker compounds in OGT preparations was quantitatively performed by high-performance liquid chromatography-photodiode array detection analysis. The marker compounds were separated on a reversed-phase C ₁₈ column and the analytical method was successfully validated, which was applied to compare OGT extracts from laboratory preparation and commercial OGT granules. Results : Limit of detection and limit of quantification values were in the ranges of $0.001-0.016{\mu}g/mL$ and $0.003-0.047{\mu}g/mL$, respectively. Precision was 0.03-3.71 % within a day and 0.03-3.81 % over four consecutive days. Recovery of marker compounds ranged from 90.63-108.26 %, with relative standard deviation (RSD) values < 4.0 %. Reproducibility was < 2.5 % of the RSD value. The 18 marker compounds were stable within 16 h at $10^{\circ}C$, with the RSD value < 3.5 %. Quantitative analysis results showed that the quantities of the 18 marker compounds varied among OGT samples. Pearson coefficient evaluation and principal component analysis demonstrated that an OGT water extract produced by a laboratory method clearly differed from commercial OGT granules. Conclusions : The developed analytical method was simple, precise, and reliable. Therefore, it can be used for the quality assessment of OGT preparations.

• Food Science of Animal Resources
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• v.36 no.4
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• pp.463-468
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• 2016

Contamination by foodborne pathogens and mycotoxins was examined in 475 eggs and 20 feed samples collected from three egg layer farms, three egg-processing units, and five retail markets in Korea. Microbial contamination with Salmonella species, Escherichia coli, and Arcobacter species was examined by bacterial culture and multiplex polymerase chain reaction (PCR). The contamination levels of aflatoxins, ochratoxins, and zearalenone in eggs and chicken feeds were simultaneously analyzed with high-performance liquid chromatography coupled with fluorescence detection after the post-derivatization. While E. coli was isolated from 9.1% of eggs, Salmonella species were not isolated. Arcobacter species were detected in 0.8% of eggs collected from egg layers by PCR only. While aflatoxins, ochratoxins, and zearalenone were found in 100%, 100%, and 85% of chicken feeds, their contamination levels were below the maximum acceptable levels (1.86, 2.24, and 147.53 μg/kg, respectively). However, no eggs were contaminated with aflatoxins, ochratoxins, or zearalenone. Therefore, the risk of contamination by mycotoxins and microbes in eggs and chicken feeds is considered negligible and unlikely to pose a threat to human health.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.2
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• pp.187-192
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• 2002

The open-connect type eddy current transducer for the flaw detection in continuously connected pipelines was developed. This eddy current transducer is for the on-line inspection of the tubes in industries, to which commercial encircling probes are not applicable. The excitation coil that consists of a ribbon type cable and a flat connector can be opened and closed on purpose. The sensing coils of this transducer are circumferentially arrayed near the outside of the tube wall but axially displaced from the exciter by about one and half tube diameter. In application to steel tubes, and the performance of this transducer was evaluated as a little behind those of magnetic saturation type in signal to noise ratio and flaw size decision, but usable to detect or to locate large size flaws in steel tubes. Surface cracks deeper than 19% of the tube thickness could be detected with good signal to noise ratio.

• Molecules and Cells
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• v.40 no.10
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• pp.714-730
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• 2017

Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

• Journal of Chest Surgery
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• v.31 no.1
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• pp.7-12
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• 1998

The study in detection of perioperative myocardial infarction by serial ECGs and the analysis of risk factors involved was carried out from January 1994 to July 1996 on 87 consecutive patients undergoing coronary artery bypass grafting. There were significant differences in the mean CK-MB peaks and frequencies of flipping in LDH1/LDH2 among the 3 groups(group I: new Q-wave, group II: S-T change, group III: no ECG change). The ECG was considered positive for postoperative myocardial infarction if the new Q-waves appeared in the postoperative period or if S-T segment changes persisted for more than 48 hours. The hospital mortality rate was 3.3% and the perioperative infarction rate was 17.2%. The following risk factors of the perioperative MI were found: endarterectomy, decreased ejection fraction($\leq$40%) and prolonged aortic cross clamping time. Left main disease, triple vessel disease, 3 or more graft, unstable angina and hypertension did not correlate with myocardial infarction. This study suggests that serial ECGs could be used as means of detecting the perioperative myocardial infarction after coronary artery bypass grafting.