• Title/Summary/Keyword: group contribution method

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RNase Resistant RNA in the Egg of Xenopus laevis: I. RNA Extraction and in Vitro Labeling

  • Chung, Hae-Moon
    • The Korean Journal of Zoology
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    • v.20 no.1
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    • pp.9-18
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    • 1977
  • RNA was extracted from the eggs of Xenopus laevis to do preliminary experiments before testing the possibility that if RNase resistant RNA molecules exist in the amphibian egg. Chromatography on Sephadex G-100 column indicated 3 peaks consistently. Only high molecular weight RNA species eluted in the first peak were labeled in vitro using $^{3}H$-dimethyl sulfate to eliminate the possible contribution of base paired oligonucleotides from tRNA. By this method, high specific activity could be obtained and the attached methyl groups were quite stable.

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Demand-based charging strategy for wireless rechargeable sensor networks

  • Dong, Ying;Wang, Yuhou;Li, Shiyuan;Cui, Mengyao;Wu, Hao
    • ETRI Journal
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    • v.41 no.3
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    • pp.326-336
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    • 2019
  • A wireless power transfer technique can solve the power capacity problem in wireless rechargeable sensor networks (WRSNs). The charging strategy is a wide-spread research problem. In this paper, we propose a demand-based charging strategy (DBCS) for WRSNs. We improved the charging programming in four ways: clustering method, selecting to-be-charged nodes, charging path, and charging schedule. First, we proposed a multipoint improved K-means (MIKmeans) clustering algorithm to balance the energy consumption, which can group nodes based on location, residual energy, and historical contribution. Second, the dynamic selection algorithm for charging nodes (DSACN) was proposed to select on-demand charging nodes. Third, we designed simulated annealing based on performance and efficiency (SABPE) to optimize the charging path for a mobile charging vehicle (MCV) and reduce the charging time. Last, we proposed the DBCS to enhance the efficiency of the MCV. Simulations reveal that the strategy can achieve better performance in terms of reducing the charging path, thus increasing communication effectiveness and residual energy utility.

EMI based multi-bolt looseness detection using series/parallel multi-sensing technique

  • Chen, Dongdong;Huo, Linsheng;Song, Gangbing
    • Smart Structures and Systems
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    • v.25 no.4
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    • pp.423-432
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    • 2020
  • In this paper, a novel but practical approach named series/parallel multi-sensing technique was proposed to evaluate the bolt looseness in a bolt group. The smart washers (SWs), which were fabricated by embedding a Lead Zirconate Titanate (PZT) transducer into two flat metal rings, were installed to the bolts group. By series connection of SWs, the impedance signals of different bolts can be obtained through only one sweep. Therefore, once the loosening occurred, the shift of different peak frequencies can be used to locate which bolt has loosened. The proposed multi input single output (MISO) damage detection scheme is very suitable for the structural health monitoring (SHM) of joint with a large number of bolts connection. Another notable contribution of this paper is the proposal of 3-dB bandwidth root mean square deviation (3 dB-RMSD) which can quantitatively evaluate the severity of bolt looseness. Compared with the traditional naked-eye observation method, the equivalent circuit based 3-dB bandwidth can accurately define the calculation range of RMSD. An experiment with three bolted connection specimens that installed the SWs was carried out to validate our proposed approach. Experimental result shows that the proposed 3 dB-RMSD based multi-sensing technique can not only identify the loosened bolt but also monitor the severity of bolt looseness.

Linear Free Energy Relationship on the Phosphorylation of Acetylcholinesterase by Insecticidal O,O-Diethylphenylphosphate Derivatives (살충성(殺蟲性) O,O-Diethylphenylphosphate유도체(誘導體)들에 의(依)한 Acetylcholinesterase의 Phosphorylation에 미치는 자유(自由)에너지 관계(關係))

  • Sung, Nack-Do
    • Korean Journal of Agricultural Science
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    • v.11 no.1
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    • pp.176-181
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    • 1984
  • Linear free energy relation ship(LFER) on the insecticidal activity of O,O-diethylphenylphosphate (A) and 3,5-dimethylphenyl-O,O-diethylphosphate (B) derivatives were studied by EHT MO calculation method and regression analysis method. LFER between varying substituent constants and $pI_{50}$ constants of phosphates, (A) & (B) were calculated with applying Hammett, Okamoto-Brown, Taft and Swain-Lupton's DSP equations;percent resonance effect(R) and field effect(F) of (A) were %R=33.5 & %F=66.5 and also that of (B) were %R=2 & %F=98, respectively. On the basis of above findings, the insecticidal activities were similar for both (A) and (B), but (B) have larger field and inductive contribution than (A), due to the 3,5-dimethyl group of (B).

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THE STUDY ON ANTERIOR DISPLACEMENT OF DISK IN PATIENTS OF TEMPOROMANDIBULAR JOINT INTERNAL DERANGEMENT IN MAGNETIC RESONACE IMAGING (측두하악관절 내장증 환자의 자기공명영상에서 관절 원판의 전방 이동에 관한 연구)

  • Moon, Chang-Soo;Cho, Byoung-Ouck;Lee, Yong-Chan;Lee, Han-Ouck;You, Han-Shick;Lee, Yul
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.15 no.3
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    • pp.189-197
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    • 1993
  • Tempormandibular Joint pain and dysfunction is a common and important clinical problem. With the recent advances in imaging technology, radiologists have made major contribution to the understanding of TMJ disease. The MRI has several advantages over the conventional imaging methods. It is possible to obtain surprisingly precise images of internal hard and soft tissues with MRI. It is not invasive without ionizing radiation. The abnormal disk position has been thought as the cause of TMJ internal derangement. But there are few methods to relate disk position to TMJ internal derangement quantatively. The object of our study is to determine the amount of anterior displacement fo articular disk in symptomatic patients related each symptoms. Using the method of Dr. Drace, we studied the 38 joints of 22 persons with susceptable TMJ internal derangement. 1. In determining the anterior displacement of TMJ articular disk, the junction between the posterior band and bilaminar zone was useful. 2. The mean anterior displacement of disk in reduction group and without reduction group were $51.0^{\circ}C$ and $81.1^{\circ}C$ respectively. The difference was significant. 3. In the mean anterior displacement of disk, the pain without clicking group showed $84.8^{\circ}C$ and the clicking and pain group $70.8^{\circ}C$, the clicking group respectively.

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The availability for cardiorespiratory fitness measurement by 20 m shuttle run test in different sports type of elite athletes. Exercise Science (엘리트 선수들의 운동특성에 따른 20 m 셔틀런 검사의 유용성)

  • Kim, J.K.;Lee, N.J.;Lee, M.S.
    • Exercise Science
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    • v.21 no.2
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    • pp.183-190
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    • 2012
  • This study is to evaluate the availability of cardiorespiratory fitness measurement by 20 m shuttle run test based upon energy contribution rates of elite athletes in different sports type. Sixty-seven elite athletes attending K national university participated in this study. They were divided by three groups based upon sports type, composed of Anaerobic Group (sprint, jumps, weightlifting, throw; n=35), Aerobic Group (medium-long distance; n=9), and Combat Sport Group (judo; n=23). 20 m shuttle run test was conducted by Leger et al.(1982) method and calculating acceleration using measured shuttle run repetitions was conducted by Brewer et al.(1988) method. To test the usefulness of VO2max, graded exercise treadmill test was conducted and standing long jump and 50 m run were measured as power fitness factors. Z-jump was used for measuring power, agility, and muscular endurance. Standing long jump and 50 m run of Anaerobic Group (AnG) was significantly higher than that of Aerobic Group (AeG) and Combat Sport Group (CG) (p<0.05). However, Z-jump of CG was significantly higher than that of AnG and AeG(p<.05). There was a higher correlation of 20 m shuttle run test and VO2max in AnG(r= 0.577, p<.0001) and CG(r= 0.760, p<.0001). Otherwise, there was a low correlation of 20 m shuttle run test and VO2max in AeG. There was no significant group difference to test the availability of 20 m shuttle run test and there was a reduced error when converting 20 m shuttle run results into VO2max. This study examined the usefulness of 20 m shuttle run test by converting 20 m shuttle run repetition results into VO2max calculation, which showed reduced error. Therefore, this study confirmed that it would be needed to convert 20 m shuttle run results into VO2max for universal and practical use in the field without dividing sports type.

Evaluation of Problem-Based Learning in an Undergraduate Nursing Course (간호학 문제 중심 학습 효과 평가 - 일개 교과목을 중심으로 -)

  • Kim Hee-Soon;Ko Il-Sun;Lee Won-Hee;Bae Seong Yeon;Shim Joung Ohn
    • Child Health Nursing Research
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    • v.10 no.4
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    • pp.395-405
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    • 2004
  • Purpose: The purpose of this research was to evaluate the application of the Problem-Based Learning(PBL) in nursing with regard to the learning process and learning outcomes. Method: PBL modules were provided to the students so that they could identify the nursing problems related to nutrition, regulation, and elimination, and implement appropriate nursing interventions according to the problems. PBL was also used to develop ability to use self-evaluation for nursing intervention outcomes. The modules were developed by the authors for the course, Nursing 1(3 credits) at Y university, and used during one semester, March to June 2003. Evaluation of the learning process and learning outcomes was done by self-report questionnaires and a semi-structured self -report developed by the authors. Result: The PBL evaluation included role of the facilitators, group work process, and self-directed learning attitude. The students evaluated the facilitator's role positively in fostering positive interactions and cooperative study among students and stimulating students to apply various problem-solving strategies. Students evaluated their own group work performances as a good opportunity to improve their group work participation, contribution, cooperation, and leadership through the PBL session. Their responsibility for their own learning and develop self-directed learning attitudes to connect present learning to long-term goals. Conclusion: Critical thinking, problem solving skills, self-directed learning and group work accomplishments in undergraduate student nurses were fostered and improved through the PBL program.

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Theoretical Estimation of Stoichiometry for Biodegradation of Hazardous Organic Compounds (유해유기물질에 대한 미생물 분해 반응식의 이론적 예측)

  • 우승한;박종문
    • Journal of Soil and Groundwater Environment
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    • v.8 no.2
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    • pp.70-77
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    • 2003
  • Theoretical estimation of overall stoichiometry for the microbial degradation of hazardous organic compounds is described. Half-reaction method based on microbial energetics was used in the theoretical estimation. In addition to the half-reaction method, other theoretical methods such as intermediate formation, oxygenation reaction, and estimation of the standard free energy of formation by group contribution theory were also applied. As a case study, the application of these methods was demonstrated for the estimation of microbial kinetics in the biodegradation of phenanthrene which was chosen as a model hazardous organic compound along with glucose and hexadecane. The cell yield, oxygen requirement, nitrogen requirement, and mineralization ratio could be estimated from the overall stoichiometry. It is believed that these theoretical estimation methods are useful tools for practical design and assessment of bioremediation of soil and groundwater contaminated with hazardous organic compounds.

Physico-chemical properties of green leaf volatiles (GLV) for ascertaining atmospheric fate and transport in fog

  • Vempati, Harsha;Vaitilingom, Mickael;Zhang, Zenghui;Liyana-Arachchi, Thilanga P.;Stevens, Christopher S.;Hung, Francisco R.;Valsaraj, Kalliat T.
    • Advances in environmental research
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    • v.7 no.2
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    • pp.139-159
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    • 2018
  • Green Leaf Volatiles (GLVs) is a class of biogenically emitted oxygenated hydrocarbons that have been identified as a potential source of Secondary Organic Aerosols (SOA) via aqueous oxidation. The physico-chemical properties of GLVs are vital to understanding their fate and transport in the atmosphere via fog processing, but few experimental data are available. We studied the aqueous solubility, 1-octanol/water partition coefficient, and Henry's law constant ($K_H$) of five GLVs at $25^{\circ}C$: methyl jasmonate, methyl salicylate, 2-methyl-3-buten-2-ol, cis-3-hexen-1-ol, and cis-3-hexenyl acetate. Henry's law constant was also measured at temperatures and ionic strengths typical of fog. Experimental values are compared to scarcely-available literature values, as well as estimations using group and bond contribution methods, property-specific correlations and molecular dynamics simulations. From these values, the partition coefficients to the air-water interface were also calculated. The large Henry's law constant of methyl jasmonate ($8091{\pm}1121M{\cdot}atm^{-1}$) made it the most significant GLV for aqueous phase photochemistry. The HENRYWIN program's bond contribution method from the Estimation Programs Interface Suite (EPI Suite) produced the best estimate of the Henry's constant for GLVs. Estimations of 1-octanol/water partition coefficient and solubility are best when correlating an experimental value of one to find the other. Finally, the scavenging efficiency was calculated for each GLV indicating aqueous phase processing will be most important for methyl jasmonate.

The Effects of Milk Group Intake to Dietary Diversity Score and Nutrient Adequacy Ratio among Toddler (유아의 우유군의 섭취가 식이의 다양성과 영양소 섭취의 적정도에 미치는 영향)

  • 권지영;박혜련;황은미
    • Journal of Nutrition and Health
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    • v.34 no.1
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    • pp.30-38
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    • 2001
  • This research was conduced to 1) examine the influence of milk group intake on the dietary, considering dietary diversity and nutrient adequacy, 2) identify which foods truly contribute to the intake of specific nutrients 3) point out the dietary problems of this age group. The subjects were 664(male 357, female 307) healthy toddlers of the ages of 12-36 months, who had neither been taking any medicine-type supplements nor had any diseases that affect the dietary intakes, chosen among the outpatients of university and private hospitals in Seoul and Kyonggi province during Mach-August, 1999. The 24 hour recall method and CAN Pro(food analysis program) were used to analyze the amount of nutrient intake. The grouping of the subjects for the analysis by monthly age was based on the 98 Korean Child growth standard. The results that the milk group intake showed significant negative correlations with the grain group(r=-0.194, p<0.01), the vegetable group(r=-0.115, p<0.01) and the fruit group(r=-0.125, p<0.01). It also showed that the more the milk group intake, the lower the Dietary Diversity Score(r=-0.131, p<0.01), and the more the intake of raw milk, the more dificient the intake of iron(r=-0.211, p<0.01), vitamin A(r=-0.169,p<0.01), vitamin B$_1$(r=-0.078, p<0.05) and vitamin C(r=-0.187, p<0.01). Foods contributory to nutrient intake for of 12-14 months of were mostly infant formula, and the contribution ratio was high. In the age of 15-17 months, the subjects obtained most of the nutrients from raw milk., liquid-type yoghurt, soybean milk, cooked rice, etc. As for iron intake, the age of 21-23 month obtained the most from ionic drinks, and 24-29 months from raw milk, chocolate cakes, ionic drinks, etc. Though the NAR(Nutrient Adequacy Ratio) and MAR(Mean Adequacy Ratio) generally tended to increase accordingly with the DDs, as for nutrients Ca, vitamin A, vitamin B$_1$, and vitamin C the NAR marked the highest when the DDS marked the lowest(due to infant formula). And for iron, even when the DDS was 5 the NAR value was low. Im conclusion, the more the milk group intake, the lower was the dietary diversity. High dependency on infant formula and others from milk group over the age of 12 months proved to result in a decrease of nutritious solid food intake, iron deficiency and a low dietary diversity score, which can lead to an unbalanced diet. Therefore, nutrition education towards mothers/caretakers of toddler is of urgent need, in order to reduce the fluid milk group intake of low nutritional density and to provide various solid foods the children. (Korean J Nutrition 34(1):30-38, 2001)

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