• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.228-234
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• 2023

In this study, the performance of a gold biosensor combined with light microscope imaging system (GB-LMIS) was comparatively evaluated against enzyme-linked immunosorbent assay (ELISA) for detecting Salmonella under simulated chilling condition. The optimum concentration of antiSalmonella polyclonal antibodies (pAbs) was determined to be 12.5 and 100 ㎍/ml for ELISA and GBLMIS, respectively. GB-LMIS exhibited a sufficient and competitive specificity toward three tested Salmonella among only. To mimic a real-world situation, chicken was inoculated with Salmonella cocktail and stored under chilling condition for 48 h. The overall growth of Salmonella under chilling condition was significantly lower than that under non-exposure to the chilling condition (p < 0.05). No significant differences in bacterial growth were observed between brain heart infusion and brilliant green broth during the enrichment period (p > 0.05). Finally, both GB-LMIS and ELISA were employed to detect Salmonella at every 2-h interval. GB-LMIS detected Salmonella with a competitive specificity by the direct observation of bacteria on the sensor using a charge-coupled device camera within a detection time of ~2.5 h. GB-LMIS is a feasible, novel, and rapid method for detecting Salmonella in poultry facilities.

• Advances in nano research
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• 제5권3호
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• pp.253-260
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• 2017

We report a simple eco-friendly process for the synthesis of gold nanoparticles (AuNPs) using aqueous extract from Coffea Arabica fruit. The formation of AuNPs was confirmed using absorption spectroscopy and scanning electron microscopy images. FT-IR analysis demonstrates the major functional groups present in Coffee Arabica fruit extract before and after synthesizing AuNPs. The Face Center Cubic (FCC) polycrystalline nature of these particles was identified by X-Ray diffraction (XRD) analysis. Taking into account the contribution of the biomass surrounding the AuNPs, dynamic light scattering (DLS) results revealed an average particle size of ~59 nm.

• 한국환경과학회지
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• 제24권9호
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• pp.1211-1220
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• 2015

Soil is the most important factor in natural environment for bio-diversity. Urbanization and development of city devastate urban soil by the fraternization of green network and run off pollution. In these facts, preservation of soil is the main issue in maintain of quality urban environment. In order to handle this issue, the gold network that link fragment soil patches is considered in maintain quality soil. This study researched the infiltration Treebox design technique based on the Low Impact Development. This technique suggest reduction of impervious area of the soil due to urbanization. The main concept of this study is encourage more permeable surfaces in urban area by using a infiltration planter. The function of the planter is hold run off as much as possible from intensive rainfall, and utilizes it in drought season. Also, this planter provides fertile soil for organism habitat by keeping appropriate moisture supplying.

• 한국초지조사료학회지
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• 제41권2호
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• pp.110-118
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• 2021

본 연구는 시화간척지에서 하계 사료작물의 초종 및 품종이 생육특성 및 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 시험에 공시된 하계사료작물은 옥수수, 수수×수단그라스 교잡종 및 기장이었다. 시험에 공시된 각각의 품종은 옥수수는 광평옥, P15453, P1952 및 P2088을 이용하였고, 수수×수단그라스 교잡종은 877F, Green star, Honey chew 및 Turbo gold 품종을 이용하였다. 기장은 국립식량과학원에서 육성한 이백찰, 금실찰 및 만홍찰 품종을 이용하였다. 사료작물의 파종은 옥수수와 수수×수단그라스 교잡종은 2019년 5월 21일 그리고 기장은 6월 4일에 하였으며 수확은 9월 2일에 하였다. 옥수수의 초장 및 착수고는 품종간에 유의성이 없었으며 수량은 P1543 품종이 가장 많았고 P2088 품종이 가장 적었으나 처리간 유의성은 나타나지 않았다. 수수×수단그라스 교잡종은 877F의 초장이 가장 컸으며 Turbo gold 품종이 가장 작았다(p<0.05). 건물수량은 877F가 3,862 kg/ha로 가장 많았고 Green star가 2,669 kg/ha로 가장 낮았다(p<0.05). 기장의 초장은 이백찰이 138cm로 가장 컸으며 나머지 품종은 유의적인 차이가 없었다. 기장의 생초수량은 평균 15,778 kg/ha로 옥수수나 수수×수단그라스 교잡종보다 많았으며, 건물수량은 평균 4,780 kg/ha이었으며 이백찰 품종이 다른 품종들에 비해 가장 많았다(p<0.05). 옥수수의 사료가치에서 TDN 함량은 P2088품종이 가장 높았고 광평옥이 가장 낮았다(p<0.05). 수수×수단그라스 교잡종은 Green star 및 Honey chew 품종이 유의적으로 높았으며 RFV 값은 Turbo gold가 가장 낮았다. 기장은 평균 조단백질 함량은 7.03%이었고 TDN 및 RFV 값은 금실찰이 64.36% 및 106으로 가장 높았다. 염농도에 따른 하계 사료작물의 발아율 조사에서 옥수수는 0.4%까지는 83.1%의 발아율을 나타내었다. 특히 P2088 및 P1921 품종은 0.6% 농도에서도 발아율이 80% 이상이었다. 수수×수단그라스 교잡종은 877F 품종의 발아율이 0.8% 농도에서도 93.3% 이었으며 Honey chew는 88.3%로 나타나 다른 품종에 비해 염 농도에 대한 저항성이 높은 것으로 나타났다. 기장은 0.6%의 염 농도에서도 84.0-88.7%의 높은 발아율을 나타내었다. 이상의 결과를 종합하여 볼 때 염농도가 높은 시화 간척지에서 가장 적합한 초종은 기장이 추천되었으며, 이백찰은 수량이 높아 적합한 품종으로 권장되었다.

• KIEAE Journal
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• 제14권5호
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• pp.21-28
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• 2014

To improve environmental problem as globally climate changes, domestic and foreign government have been trying to reduce green gas emitted by all industries. With making the green building certification system that assess the substantiality and energy performance of building, a governments have been using by a way for reducing green gas emitted in building industry. G-SEED(Green Standard for Energy & Environmental Design) developed in Korea have been reinforcing, and a number of projects certifying the G-SEED have been increasing continuously. As a demand of G-SEED certification is rising, a question on the additional cost data as certifying G-SEED is rising. It is because additional cost as getting the certification is important fact for G-SEED level decision and whether getting the certification or not. Therefore, this study analyzed additional construction cost as certifying G-SEED through performance improvement and design change of general office building not to get G-SEED. In conclusion, an additional construction cost ratio of G-SEED projects to the reference building is drawn as certified level; +0.26%, silver level; +2.29%, gold level; +3.89%, and platinum level; +5.48%.

• 한국환경과학회지
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• 제29권12호
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• pp.1171-1184
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• 2020

The green synthesis of inorganic nanoparticles (NPs) using biomaterials has garnered considerable attention in recent years because of its eco-friendly, non-toxic, simple, and low-cost nature. In this study, we synthesized NPs of noble metals, such as Ag and Au using an aqueous extract of a marine seaweed, Ecklonia cava. The formation of AgNPs and AuNPs was confirmed by the presence of surface plasmon resonance peaks in UV-Vis absorption spectra at approximately 430 and 530 nm, respectively. Various properties of the NPs were evaluated using characterization techniques, such as dynamic light scattering, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. Phytochemicals in the seaweed extract, such as phlorotannins, acted as both reducing and stabilizing agents for the growth of the NPs. The green-synthesized AgNPs and AuNPs were found to exhibit high catalytic activity for the decomposition of organic dyes, including azo dyes, methylene blue, rhodamine B, and methyl orange.

• 자원환경지질
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• 제30권4호
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• pp.279-289
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• 1997

Dark to pale green-colored, Cr-bearing sericites from hydrothermal alteration zone of the Narim gold deposit were investigated mineralogically and geochemically. The alteration zone is composed mineralogically of quartz, carbonate minerals and green sericite with minor amounts of chlorite, barite and sulfide minerals (pyrite, sphalerite, galena). The zone is enriched in As (967 to 1520 ppm), Cu (31 to 289 ppm), Ni (1027 to 1205 ppm), Pb (0.20 to 1.24 wt.%) and Zn (1.03 to 1.07 wt. %) compared with fresh rocks such as granitic gneiss, porphyritic biotite granite and basic dyke. The Cr, probably the chromophore element, is highly enriched in the alteration zone (1140 to 1500 ppm), host granitic gneiss (1200 ppm) and porphyritic biotite granite (1200 ppm). Occurrence and grain size of sericite are diverse, but most of the Cr-bearing sericites (150 to $200{\mu}m$ long and 20 to $30{\mu}m$ wide) occur along the boundaries between ore veins and host rocks (especially basic dyke and granitic gneiss). X-ray diffraction data of the sericite show its monoclinic form with unit-cell parameters of $a=5.202{\AA}$, $b=8.994{\AA}$, $c=20.103{\AA}$, ${\beta}=95.746^{\circ}$ and $V=935.83{\AA}^3$, which are similar with the normal 2M1-type muscovite. Representative chemical formula of the sericite is ($K_{1.54}Ca_{0.03}Na_{0.01}$)($Al_{3.42}Mg_{0.38}Cr_{0.14}Fe_{0.06}V_{0.02}$)($Si_{6.69}Al_{1.31}$)$O_{20}(OH)_4$. The Cr content increases with decrease of the octahedral Al content, and ranges from 0.36 to 2.58 wt.%. DTA and TG curves of the sericite show endothermic peaks at $342^{\circ}$ to $510^{\circ}$, $716^{\circ}$ to $853^{\circ}$ and $1021^{\circ}C$, which are due to the expulsion of hydroxyl group. The total weight loss by heating is measured to be about 8.8 wt. %, especially at $730^{\circ}C$. Infrared absorption experiments of the sericite show broad absorption band due to the O-H bond stretching vibration near the $3625cm^{-1}$, coupled with the 825 and $750cm^{-1}$ doublet. The vibration bands related with the H-O-Al and Si-O-Al bonds occur at $1030cm^{-1}$ and 500 to $700cm^{-1}$, respectively. Based on paragonite content of the sericite, the formation temperature of the Narim gold deposit is calculated to be $220{\pm}10^{\circ}C$.

• 한국문화재보존과학회:학술대회논문집
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• 한국문화재보존과학회 2007년도 제26회 추계학술대회
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• pp.33-35
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• 2007

• 국제초고층학회논문집
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• 제6권4호
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• pp.323-326
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• 2017

Sustainability is becoming more and more important in our everyday lives. Thus, it is apparent that more sustainable initiatives are incorporated in a building design concept to reduce operation costs and environmental impacts. However, will the construction cost go up if the building is going green - especially if it is a tall building? This is the question on everyone's mind. In the following paragraphs, we will look into a case study of a skyscraper in Nanning, China. That 411-metre skyscraper, Nanning China Resources Centre East Office Tower, is currently under construction. Designed to LEED-CS Gold standards, the building has adopted a number of sustainable design elements and operation practice, which indeed only accounts for a small percentage of the total construction cost.

• 보존과학회지
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• 제31권1호
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• pp.21-27
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• 2015

국보 제193호 봉수형유리병은 5세기에 조성된 것으로 알려진 황남대총 남분의 수많은 부장품들 사이에 독특한 형태의 녹색 유리 편들로 심하게 파손된 채 출토되었다. 1984년 국립중앙박물관 보존과학실에서 본격적으로 보존처리를 실시하여 박물관에서 오랜 기간 동안 전시되어 왔지만, 30년이라는 세월로 인해 기존에 사용한 접착제는 심하게 열화가 되어 매우 불안전한 상태가 되었으며 특히 복원제로 사용한 에폭시 수지가 빛과 열에 의해 심하게 황변이 되어 더 이상 유물의 안정한 전시와 효과적인 전시가 이루어질 수 없어 이번에 재 보존처리를 하게 되었다. 이번 연구에서는 그동안 연구가 전혀 진행되지 않았던 세 조각으로 파손된 봉수형유리병 손잡이 부분을 금사로 정성스럽게 감아 수리에 사용한 3 조각의 금사를 중점적으로 살펴보았다. 금사에 대한 분석방법으로는 SEM-EDS와 Stereo Microscope를 사용하여 비파괴적으로 분석하였다. 먼저, SEM-EDS 분석 결과, Au 91.9wt%-Au 92.8wt% 와 Ag 5.9wt.%-Ag 6.5wt.%으로 Au 과 Ag의 합금인 금제로 밝혀졌다. 다음으로 광학현미경으로 금사의 제작기법을 관찰한 결과, 일반적으로 금사는 덩이 금을 두드려서 만들거나 구멍에 밀어 넣어 뽑아내는 인발 가공 또는 금판을 꼬아서 제작하는 방법으로 제작하는데 봉수형유리병 손잡이에서 분리한 금사는 표면에서 꼬임 흔적은 전혀 나타나지 않고 단지 길이 방향으로 미세한 줄무늬가 관찰되고 단면이 모두 채워진 상태인 것으로 보아 인발 가공하여 만든 것을 사용하여 보수한 것으로 생각된다. 이러한 분석 결과는 그동안 금사에 대한 연구가 대부분 금제품 유물의 구성품으로만 인식된 채 별도의 유물로 주목받지 못해 금사에 대한 제작기법에 대한 체계적인 연구가 매우 미흡한 편이기 때문으로 장차 금사에 대한 추가적인 연구가 진행된다면 이번 봉수형유리병 손잡이 고정에 사용된 금사 분석 결과가 기존 신라시대 금제 유물의 금사와의 상호 연관성을 밝혀내는데 좋은 자료가 될 것으로 생각된다.