• Title/Summary/Keyword: green fusion technology

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A study on Process Characteristics Using Fast Tool Servo based Surface Texturing (FTS 를 이용한 표면처리 방법에 따른 공정특성 연구)

  • Lee, Seung Jun;Lee, Deug Woo;Kim, Jong-Man;Lee, Sang Min;Kim, Mi Ru;Jang, Nam-Su;Li, Liang
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.12
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    • pp.1127-1132
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    • 2014
  • Fast tool servo (FTS) is an enabling technology to fabricate various shapes of functional surface geometries in a precise and controllable manner. FTS can be also employed as a straightforward and efficient surface treatment way of making such products more durable. In this work, process characteristics using high-precision FTS-based surface texturing were qualitatively and quantitatively investigated to provide a class of surface design rule. The morphologies of surfaces processed with different conditions were first examined by observing the resultant 2D/3D surface profiles. In addition, the effects of the surface treatment using FTS on hardness and wear properties were characterized and compared to those without treatment.

Homologous Expression and T3SS-Dependent Secretion of TAP-Tagged Xo2276 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract and Its Direct In Vitro Recognition of Putative Target DNA Sequence

  • Kim, Seunghwan;Nguyen, Thi-Dieu-Hanh;Lee, Joohee;Hong, Myoung-Ki;Pham, Tan-Viet;Ahn, Yeh-Jin;Lee, Byoung-Moo;Han, Ye Sun;Kim, Dong-Eun;Kim, Jeong-Gu;Kang, Lin-Woo
    • Journal of Microbiology and Biotechnology
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    • v.23 no.1
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    • pp.22-28
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    • 2013
  • Xo2276 is a putative transcription activator-like effector (TALE) in Xanthomonas oryzae pv. oryzae (Xoo). Xo2276 was expressed with a TAP-tag at the C-terminus in Xoo cells to enable quantitative analysis of protein expression and secretion. Nearly all TAP-tagged Xo2276 existed in an insoluble form; addition of rice leaf extracts from a Xoosusceptible rice cultivar, Milyang23, significantly stimulated secretion of TAP-tagged Xo2276 into the medium. In a T3SS-defective Xoo mutant strain, secretion of TAPtagged Xo2276 was blocked. Xo2276 is a Xoo ortholog of Xanthomonas campestris pv. vesicatoria (Xcv) AvrBs3 and contains a conserved DNA-binding domain (DBD), which includes 19.5 tandem repeats of 34 amino acids. Xo2276- DBD was expressed in E. coli and purified. Direct in vitro recognition of Xo2276-DBD on a putative target DNA sequence was confirmed using an electrophoretic mobility shift assay. This is the first study measuring the homologous expression and secretion of Xo2276 in vitro using rice leaf extract and its direct in vitro binding to the specific target DNA sequence.

Chiral Mesoporous Silica for Asymmetric Metal-free Catalysis: Enhancement of Chirality thorough Confinement Space by Plug Effect

  • Jeong, Eun-Yeong;Im, Cheong-Rae;Park, Sang-Eon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.199-199
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    • 2011
  • The addition of a carbanion to ${\yen}{\acute{a}}{\yen}{\hat{a}}$-unsaturated carbonyl compounds is of importance in the C-C bond formation reactions for modern pharmaceuticals and organic synthesis. Recently, heterogeneous asymmetric catalysis became more attractive area of research because of the easy recovery and separation of the catalyst from the reaction system. Most of synthetic methods for heterogeneous catalysts were grafting or immobilization of homogeneous catalyst onto the solid supports. Trans-1,2-Diaminocyclohexane(DACH) and L-proline ligands have been enormously used as chiral ligands in several catalytic transformation under homogenous conditions. Our group prepared l-proline functionalized mesoporous silica was synthesized under acidic condition using a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer template (EO20PO70EO20, Pluronic P-123, BASF). Furthermore, we successfully directly synthesized trans-1,2 diaminocyclohexane functionalized mesoporous silica by using microwave method. The direct functionalization of chiral ligand into the framework of mesoporous materials is expected to be useful for the heterogeneous asymmetric catalysis. So, we adopt the direct synthesis of chiral ligand functionalized mesoporous silica by using thermal and microwave irradiation. Then, chiral ligand functionalized mesoporous silicas were applied to enantioselective asymmetric catalytic reactions.

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Effects of Eu3+ and Tb3+ Activator Ions on the Properties of SrSnO3 Phosphors (Eu3+와 Tb3+ 활성제 이온이 SrSnO3 형광체의 특성에 미치는 영향)

  • Kim, Jung Dae;Cho, Shinho
    • Korean Journal of Materials Research
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    • v.24 no.9
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    • pp.469-473
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    • 2014
  • $SrSnO_3$ phosphor powders were synthesized with two different contents of activator ions $Eu^{3+}$ and $Tb^{3+}$ using the solid-state reaction method. The structural, morphological, and optical properties of the phosphors were investigated using X-ray diffractometry, field-emission scanning electron microscopy, and fluorescence spectrophotometry, respectively. All the phosphors showed a cubic structure, irrespective of the type and the content ratio of activator ions. For $Eu^{3+}$-doped $SrSnO_3$ phosphors, the intensity of the 620 nm red emission spectrum resulting from the $^5D_0{\rightarrow}^7F_2$ transition of $Eu^{3+}$ was stronger than that of the 595 nm orange emission signal due to the $^5D_0{\rightarrow}^7F_1$ transition in the range 0.01-0.05 mol of $Eu^{3+}$, but the ratio of the intensity was reversed in the range 0.10-0.20 mol of $Eu^{3+}$. The variation in the emission intensity indicates that the site symmetry of the $Eu^{3+}$ ions around the host crystal was changed from non-inversion symmetry to inversion. For the $Tb^{3+}$-doped $SrSnO_3$ phosphors under excitation at 281 nm, one strong green emission band at 550 nm and several weak bands were observed. These results suggest that the optimum red and green emission signals can be realized when the activator ion content for $Eu^{3+}$- or $Tb^{3+}$-doped $SrSnO_3$ phosphors is 0.20 mol and 0.15 mol, respectively.

A Study of a Combined Microwave and Thermal Desorption Process for Contaminated Soil

  • Ha, Sang-An;Choi, Kyoung-Sik
    • Environmental Engineering Research
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    • v.15 no.4
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    • pp.225-230
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    • 2010
  • In order to treat soil contaminated with high percentages of water and petroleum, the combined microwave and thermal desorption process was studied, which was composed of the consecutive connection of two pre-treatment processes. For the thickness of the contaminated soil layer on the transfer conveyor belt, the optimal total petroleum hydrocarbon (TPH) removal rate was studied with respect to the duration of microwave exposure in the consecutive process combined with thermal desorption. The TPH removal rate when the contaminated soil layer thickness was 1 cm at 6 kW of microwave power was 80%. The removals rates for 2 and 3 cm soil layer thicknesses were both 70%. Under identical experimental conditions, the TPH removal rate for the microwave pre-treatment, when considering the soil particle size, was over 70%. The lowest TPH removal rate was achieved with a particle diameter of 2.35 mm. For contaminated soil with 30% water content, 6 kW and a thermal desorption temperature of $600^{\circ}C$ were the optimal operational conditions for the removal of THP. However, considering the fuel consumption cost, 4 kW and a thermal desorption temperature of $300^{\circ}C$ would be the most economic conditions.

Homologous Expression and Quantitative Analysis of T3SS-Dependent Secretion of TAP-Tagged XoAvrBs2 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract

  • Kim, S.H.;Lee, S.E.;Hong, M.K.;Song, N.H.;Yoon, B.;Viet, P.T.;Ahn, Y.J.;Lee, B.M.;Jung, J.W.;Kim, K.P.;Han, Y.S.;Kim, J.G.;Kang, L.W.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.7
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    • pp.679-685
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    • 2011
  • Xanthomonas oryzae pv. oryzae (Xoo) produces a putative effector, XoAvrBs2. We expressed XoAvrBs2 homologously in Xoo with a TAP-tag at the C-terminus to enable quantitative analysis of protein expression and secretion. Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels, and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium. In a T3SS-defective Xoo mutant strain, secretion of the TAPtagged XoAvrBs2 was blocked. Thus, we elucidated the transcriptional and translational expressions of the XoAvrBs2 gene in Xoo was induced in vitro by the interaction with rice and the induced secretion of XoAvrBs2 was T3SSdependent. It is the first report to measure the homologous expression and secretion of XoAvrBs2 in vitro by rice leaf extract. Our system for the quantitative analysis of effector protein expression and secretion could be generally used for the study of host-pathogen interactions.

Reduction of viewing-angle dependent color shift in a reflective type cholesteric liquid crystal color filter

  • Jang, Won-Gun;Beom, Tae-Won;Cui, Hao;Park, Jong-Rak;Hwang, Seong-Jin;Lim, Young-Jin;Lee, Seung-Hee
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.1656-1659
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    • 2008
  • The reflective type color filter for the liquid crystal displays (LCD) was produced using cholesteric liquid crystal monomers whose phase is characterized by the unique optical features of selective reflection. Periodic micrometer scale hemi-spherical photoresist (PR) patterns were formed on glass substrates by thermal reflow method after photolithography. Cholesteric color filter films for red, green and blue light reflections were then produced and the viewing angle dependence was investigated and compared with that of reflected light on the non-patterned substrates.

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A Study on Manpower Education Plan of the Green Growth Industry of SME in Gwangju Area (광주지역 중소기업의 녹색성장산업 인력양성방안에 관한 연구)

  • Yim, Ki-Heung
    • Journal of Digital Convergence
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    • v.8 no.2
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    • pp.115-129
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    • 2010
  • The purpose of this study is to explore manpower education plan of green growth industry of SME in Gwangju area. Nowadays, according to the change of green growth paradigm, We need to be the green switching strategy for solving the structural problem and overcoming a crisis in SME. Based on the findings, the study showed that SME in Gwangju area need to build up network of size and industrial classification. Also, green fusion technology grafting on the existing industry and the policy of educating manpower in long term and custom-made plan of educating manpower need to be followed.

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