Browse > Article
http://dx.doi.org/10.4014/jmb.1207.07039

Homologous Expression and T3SS-Dependent Secretion of TAP-Tagged Xo2276 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract and Its Direct In Vitro Recognition of Putative Target DNA Sequence  

Kim, Seunghwan (Department of Advanced Technology Fusion, Konkuk University)
Nguyen, Thi-Dieu-Hanh (Department of Bioscience and Biotechnology, Konkuk University)
Lee, Joohee (Department of Green Life Science, College of Convergence, Sangmyung University)
Hong, Myoung-Ki (Department of Advanced Technology Fusion, Konkuk University)
Pham, Tan-Viet (Department of Advanced Technology Fusion, Konkuk University)
Ahn, Yeh-Jin (Department of Green Life Science, College of Convergence, Sangmyung University)
Lee, Byoung-Moo (Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Han, Ye Sun (Department of Advanced Technology Fusion, Konkuk University)
Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
Kim, Jeong-Gu (Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Kang, Lin-Woo (Department of Biological Sciences, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.1, 2013 , pp. 22-28 More about this Journal
Abstract
Xo2276 is a putative transcription activator-like effector (TALE) in Xanthomonas oryzae pv. oryzae (Xoo). Xo2276 was expressed with a TAP-tag at the C-terminus in Xoo cells to enable quantitative analysis of protein expression and secretion. Nearly all TAP-tagged Xo2276 existed in an insoluble form; addition of rice leaf extracts from a Xoosusceptible rice cultivar, Milyang23, significantly stimulated secretion of TAP-tagged Xo2276 into the medium. In a T3SS-defective Xoo mutant strain, secretion of TAPtagged Xo2276 was blocked. Xo2276 is a Xoo ortholog of Xanthomonas campestris pv. vesicatoria (Xcv) AvrBs3 and contains a conserved DNA-binding domain (DBD), which includes 19.5 tandem repeats of 34 amino acids. Xo2276- DBD was expressed in E. coli and purified. Direct in vitro recognition of Xo2276-DBD on a putative target DNA sequence was confirmed using an electrophoretic mobility shift assay. This is the first study measuring the homologous expression and secretion of Xo2276 in vitro using rice leaf extract and its direct in vitro binding to the specific target DNA sequence.
Keywords
AvrBs3; TALE; DNA-binding domain; Xanthomonas oryzae pv. oryzae;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Cornelis, G. R. and F. Van Gijsegem. 2000. Assembly and function of type III secretory systems. Annu. Rev. Microbiol. 54: 735-774.   DOI   ScienceOn
2 Bai, J., S. H. Choi, G. Ponciano, H. Leung, and J. E. Leach. 2000. Xanthomonas oryzae pv. oryzae avirulence genes contribute differently and specifically to pathogen aggressiveness. Mol. Plant Microbe Interact. 13: 1322-1329.   DOI   ScienceOn
3 Boch, J. and U. Bonas. Xanthomonas AvrBs3 family-type III effectors: Discovery and function. Annu. Rev. Phytopathol. 48: 419-436.
4 Bogdanove, A. J., S. Schornack, and T. Lahaye. 2010. TAL effectors: Finding plant genes for disease and defense. Curr. Opin. Plant Biol. 13: 394-401.   DOI   ScienceOn
5 Feldman, M. F. and G. R. Cornelis. 2003. The multitalented type III chaperones: All you can do with 15 kDa. FEMS Microbiol. Lett. 219: 151-158.   DOI   ScienceOn
6 Galan, J. E. and A. Collmer. 1999. Type III secretion machines: Bacterial devices for protein delivery into host cells. Science 284: 1322-1328.   DOI   ScienceOn
7 Parsot, C., C. Hamiaux, and A. L. Page. 2003. The various and varying roles of specific chaperones in type III secretion systems. Curr. Opin. Microbiol. 6: 7-14.   DOI   ScienceOn
8 He, S. Y., K. Nomura, and T. S. Whittam. 2004. Type III protein secretion mechanism in mammalian and plant pathogens. Biochim. Biophys. Acta 1694: 181-206.   DOI   ScienceOn
9 Kay, S., S. Hahn, E. Marois, G. Hause, and U. Bonas. 2007. A bacterial effector acts as a plant transcription factor and induces a cell size regulator. Science 318: 648-651.   DOI   ScienceOn
10 Kim, S. H., S. E. Lee, M. K. Hong, N. H. Song, B. Yoon, P. Viet, et al. 2011. Homologous expression and quantitative analysis of T3SS-dependent secretion of TAP-tagged XoAvrBs2 in Xanthomonas oryzae pv. oryzae induced by rice leaf extract. J. Microbiol. Biotechnol. 21: 679-685.   DOI   ScienceOn
11 Lee, B. M., Y. J. Park, D. S. Park, H. W. Kang, J. G. Kim, E. S. Song, et al. 2005. The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice. Nucleic Acids Res. 33: 577-586.   DOI   ScienceOn
12 Marois, E., G. Van den Ackerveken, and U. Bonas. 2002. The Xanthomonas type III effector protein AvrBs3 modulates plant gene expression and induces cell hypertrophy in the susceptible host. Mol. Plant Microbe Interact. 15: 637-646.   DOI   ScienceOn
13 Salmond, G. P. C. and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in Gram-negative bacteria. Trends Biochem. Sci. 18: 7-12.   DOI   ScienceOn
14 Roden, J. A., B. Belt, J. B. Ross, T. Tachibana, J. Vargas, and M. B. Mudgett. 2004. A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection. Proc. Natl. Acad. Sci. USA 101: 16624-16629.   DOI   ScienceOn
15 Schornack, S., A. Meyer, P. Romer, T. Jordan, and T. Lahaye. 2006. Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins. J. Plant Physiol. 163: 256-272.   DOI   ScienceOn
16 Schulte, R. and U. Bonas. 1992. A Xanthomonas pathogenicity locus is induced by sucrose and sulfur-containing amino acids. Plant Cell 4: 79-86.
17 Zhu, W., B. Yang, J. M. Chittoor, L. B. Johnson, and F. F. White. 1998. AvrXa10 contains an acidic transcriptional activation domain in the functionally conserved C terminus. Mol. Plant Microbe Interact. 11: 824-832.   DOI   ScienceOn