• 제목/요약/키워드: grassland

검색결과 2,593건 처리시간 0.031초

Interference of In Vitro and In Vivo Growth of Several Intestinal Bacteria by Lactococcus Strains

  • Kimoto-Nira, Hiromi;Ohmomo, Sadahiro;Nomura, Masaru;Kobayashi, Miho;Mizumahi, Koko;Okamoto, Takashi
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권7호
    • /
    • pp.1286-1289
    • /
    • 2008
  • The ability of Lactococcus strains to inhibit the growth of intestinal bacteria was examined. In in vitro cocultures, we observed that among eighteen Lactococcus strains tested, the ability to inhibit growth of Escherichia coli varied, with the L. lactis N7 showing the greatest growth inhibition. Strain N7 ($8.94\times10^{10}$ CFU/day for 7 days) was orally administered to mice, and the viable count of strain N7 in feces appeared at a level of $10^{4-5}$ CFU/g. After administration, the proportion of Bacteroidaceae to total intestinal bacteria decreased. Lactococci may act as probiotic bacteria by inhibiting the growth of harmful bacteria.

Dietary Sesame Meal Increases Plasma HDL-cholesterol Concentration in Goats

  • Hirano, Y.;Kashima, T.;Inagaki, N.;Uesaka, K.;Yokota, H.;Kita, K.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제15권11호
    • /
    • pp.1564-1567
    • /
    • 2002
  • Influence of dietary sesame meal on plasma glucose, non-esterified fatty acid (NEFA), triglyceride, total cholesterol, high-density lipoprotein (HDL)-cholesterol and urea concentrations in goats was examined. Goats were fed a control diet (50% timothy hay and 50% concentrates) (CD) or a sesame meal diet (50% timothy hay, 25% concentrates and 25% sesame meal) (SMD) during 12 days. Blood samples were taken after overnight fasting and afternoon every day. Body weight was not changed by feeding either CD or SMD. The concentrations of plasma triglyceride and urea were higher (p<0.05) in goats fed SMD than those fed CD. Plasma NEFA concentration was higher in plasma samples after overnight fasting. Plasma glucose concentration in plasma samples collected afternoon was higher than those after overnight fasting. Plasma total cholesterol concentration was significantly increased by feeding SMD but not by feeding CD, which was due to the remarkable increase of plasma HDL-cholesterol concentration. In conclusion, dietary sesame meal brought about an increase in plasma total cholesterol concentration accompanied with an increment in plasma HDL-cholesterol consentration in goats.

Effect of addition of lactic acid bacteria on quality of rye silage harvested at early heading stage

  • Kuppusamy, Palaniselvam;Choi, Ki-Choon;Srigopalram, Srisesharam;Ilavenil, Soundharrajan;Park, Hyung-Su;Jung, Jeong Sung
    • 한국작물학회:학술대회논문집
    • /
    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
    • /
    • pp.285-285
    • /
    • 2017
  • This study is aimed to analyze the effect of lactic acid bacteria inoculants (LAB) on quality of rye silage fermentation, harvested at early heading stage. The nutritive values were similar between the control and LAB inoculated silages. The pH of rye silage in LAB inoculation significantly decreased as compared to control (p<0.05). In addition, the content of lactic acid in LAB inoculation significantly increased (p<0.05), but the content of acetic acid in LAB treatments decreased. In addition, lactic acid bacterial counts in LAB inoculation significantly increased as compared to control (p<0.05). Therefore, we suggest that rye silage could be improved by novel lactic acid inoculation.

  • PDF

Comparison of Agrobacterium-mediated of Five Alfalfa (Medicago sativa L.) Cultivars Using the GUS Reporter Gene

  • Lee, Sang-Hoon;Kim, Ki-Yong;Park, Hyung Soo;Cha, Joon-Yung;Lee, Ki-Won
    • 한국초지조사료학회지
    • /
    • 제34권3호
    • /
    • pp.187-192
    • /
    • 2014
  • Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

지표추파법에 의한 목야지 개량시 선점식생과 시비의 영향 (The Effects of Existing Vegetation and Fertilization on the Improvement of Natural Grassland by Oversowing)

  • 김동암
    • 한국초지조사료학회지
    • /
    • 제1권1호
    • /
    • pp.2-9
    • /
    • 1978
  • A review of factors influencing grass and clover establishment, survival and yield at oversowing was made from the experimental results of home and abroad. The following conclusions are considered: (1) The existing vegetation present at oversowing appeared to be the most critical factor reducing establishment and survival of grass. Therefore, it is essential to check competition from the native vegetation before oversowing. (2) Although lime had comparatively little effect on yield of grassland, the general effect of lime should be more emphasized under our acid soil conditions to promote the availability of all the essential elements and the growth of microorganisms, and to reduce the toxic effects of nutrients. One to two tons of lime per ha at oversowing would be useful. (3) Phosphorus is one of the nutrients most generally deficient in grassland soils of Korea, consequently, this nutrients applied at oversowing is very effective. Application as much as 200kg of phosphorus per ha would be essential. (4) The effect of nitrogen on the establishment and survival of grass depends on the amount and density of the herbage present. The use of nitrogen in dense herbage adversely affected grass establishment and survival, possible because the fertilizer stimulated the growth of the eisting herbage. Around 40kg of nitrogen per ha may be enough at oversowing (5) Potassium is not as universally deficient in soils of native grassland as phosphorus. Therefore, it cannot be over-emphasized at oversowing. Studies determinig the optimum amount of potassium at of oersowing are needed.

  • PDF

초지류형별(草地類型別) 방목축(放牧畜)에 의한 목초이용(牧草利用)과 방목(放牧) 및 사회습성(社會習性) 비교(比較) (A Comparison of Herbage Use, Grazing and Social Behaviour by Livestock Grazing under Grassland Types)

  • 이인덕;명전;송우석;임동찬
    • 농업과학연구
    • /
    • 제13권2호
    • /
    • pp.219-226
    • /
    • 1986
  • 평지(平地), 경사(傾斜) 및 임간초지(林間草地)의 유형(類型)이 가축(家畜)에 의한 목초이용(牧草利用)과 방목(放牧) 및 사회습성(社會習性)에 미치는 영향(影響)을 비교검토(比較檢討)하고자 4년차(年次) 기존(旣存) 혼파초지(混播草地)에서 한우육성빈우(韓牛育成牝牛) 30두(頭)와 재래산양(在來山羊) 5두(頭)를 공시(供試)하여 시험(試驗)한 결과(結果)는 다음과 같다. 1. 재래산양(在來山羊)에 의한 상대섭취빈도(相對攝取頻度)는 평지초지(平地草地)가 35.2%, 경사초지(傾斜草地)가 35.0%, 임간초지(林間草地)가 29.8%이었고, 두당(頭當) 1일(日) 건물섭취량(乾物攝取量)은 평지초지(平地草地)가 462g, 경사초지(傾斜草地)가 459g, 임간초지(林間草地)가 391g이었으며 건물소화율(乾物消化率)은 평지초지(平地草地)가 63.7% 경사초지(傾斜草地) 63.0% 임간초지(林間草地)가 62.1%이었다. 2. 한우(韓牛)에 의한 채식시간(採食時間)은 초지류형(草地類型)에 따라 차이(差異)가 없었으나 반추시간(反芻時間)은 경사초지(傾斜草地)에서 길었고, 임간초지(林間草地)에서 짧았다. 휴식시간(休息時間)은 임간초지(林間草地)에서 길었고 경사초지(傾斜草地)에서 짧았다. 보행시간(步行時間)은 평지초지(平地草地)에서 길었고, 방황시간(彷徨時間)은 임간초지(林間草地)에서 길었다. 반추회수(反芻回數), 식괴당저작회수(食塊當咀嚼回數) 및 배분회수(排糞回數)는 임간초지(林間草地)에서 적었고, 배뇨회수(排尿回數)는 임간초지(林間草地)에서 많았다. 음수인수(飮水因數) 및 음수량(飮水量)은 경사초지(傾斜草地)에서 많았고 보행거리(步行距離)는 경사초지(傾斜草地)에서 길었다. 3. 한우(韓牛)에 의한 사회적(社會的) 거리(距離)는 임간초지(林間草地)가 3.70m, 경사초지(傾斜草地)가 3.56m, 평지초지(平地草地)가 3.41m였고, 두당점유면적(頭當占有面積)은 임간초지(林間草地)가 $14.6m^2$, 평지초기(平地草地)가 $11.9m^2$, 경사초지(傾斜草地)가 $11.0m^2$이었다. 우군형성(牛群形成)은 경사초지(傾斜草地)가 3.7두(頭), 평지초지(平地草地)가 3.6두(頭), 임간초지(林間草地)가 3.4두(頭)이었다. 방목이동순위(放牧移動順位)는 복합적(複合的)인 pear-shaped grazing formation에 가까웠으나 초지유형(草地類型)에 따른 상하(上下) 방목축간(放牧畜間)의 우열관계(優劣管係)는 분명(分明)치 않았다.

  • PDF

초지에 대한 인산질비료의 시용에 관한 연구 II. 초지조성 및 관리시에 인산질비료의 시용이 초지식생 및 목초의 무기물 함량에 미치는 영향 (Studies on the Phosphatic Fertilizer Application in Grassland II. Effects of phosphatic fertilizer application at the establishment and management of grassland on the botanical composition and mineral content of grasses)

  • 박근제;이필상;최기준;황석중
    • 한국초지조사료학회지
    • /
    • 제13권4호
    • /
    • pp.268-273
    • /
    • 1993
  • 본 시험은 초지조성시와 관리시에 시용하는 인산질 비료의 시용이 초지식생, 목초 및 토양의 무기물함량에 미치는 영향을 구명코자 무시용구의 3처리를 난괴법 3반복으로 포장 배치하여 1989년 9월부터 1991년 10월까지 수원 근교에서 수행되었던 바, 그 결과를 요약하면 다음과 같다. 1. 인산질 비료 무시용구의 식생은 불량하였으며 인산시용에 의해서 두과 목초의 비율이 증가되었는데 특히 초지관리시의 인산효과가 현저하였다. 2. 목초의 무기물 함량중 질소, 인, 칼슘, 마그네슘 등은 인산 시용구에서 증가되었고 K/Ca+Mg 당량비는 낮아졌으나, K 및 Ca/P는 일정한 경향을 보이지 않았다. 3. 인산 시용에 의해 표토(0∼10cm)의 유효인산함량. Ca및 Mg의 치환용량은 현저히 증가되었으나 칼륨은 일정한 경향이 없었다. 4. 산지 초지조성 및 관리시에 인산질 비료의 시용은 필수적이며 특히 초지관리시의 지속적인 시용은 조성시의 무시용을 거의 보완할 수 있었다.

  • PDF

페레니얼 라이그라스 위주 초지에서 목초, 잡초 식생 군락간 상호작용 (The Interaction Index Between Grasses and Weeds in the Grassland Composed with Mainly Perennial Ryegrass)

  • 임근발;성병렬;이현준;안병석;서성
    • 한국초지조사료학회지
    • /
    • 제26권2호
    • /
    • pp.105-112
    • /
    • 2006
  • 본 시험은 페레니얼라이그라스 위주 초지에서 목초 및 잡초의 건물중의 분포와 기능 및 목초와 잡초간 경합에 의한 상호작용을 조사하였다. 페레니얼라이그라스 위주 초지를 조파조성 및 조파 조성후 보파 관리하여 연 3회 예취와 방목이용하고 이 초지에서 발생량이 많은 잡초인 냉이, 바랭이와 피에 대하여 조파 및 보파한 채초지와 방목지에서 목초와 각 잡초간 상호작용을 측정하였다. 목초와 잡초간 상호작용의 측정을 위해 기대수량(expected yield : P), 상대지수(relative yield total : RYT), 보상지수(compensation index: CI) 및 형태지수 (morphological index: MI) 등 지수(Index)를 도입하였다. 도입된 이들 지수의 측정은 봄, 여름 및 가을의 각 예취 직전에 이루어졌는데 측정된 각 지수는 각 처리구별 예취 시기별 및 잡초 종류 따른 차이와 변화가 인정되었다. 기대수량의 경우 가을 이용시기에 보파에 의해 약간 증가하였으나 상대수량에 있어 0.17에서 0.41 범위에 있어 크게 개선되지는 않는 것으로 나타났다. 보상지수는 -0.3에서 -0.6 범위로 모두 과소보상(+, --)으로 나타나 잡초에 의한 수량감소가 크게 이루어진 것으로 나타났다. 페레니얼라이그라스 위주 초지에서 보상지수를 감소시키는 주요 잡초인 바랭이, 피의 경우 방목이용에 따른 페레니얼 위주 초지의 개선효과 보다 조파 및 조파 후 보파관리에 의한 개선효과는 크지 않았다.

Intake, Digestibility In vivo, N Utilization and In sacco Dry Matter Degradability of Grass Silage Harvested at Three Stages of Maturity

  • Vranic, Marina;Knezevic, Mladen;Perculija, Goran;Bosnjak, Kresimir;Leto, Josip
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제22권2호
    • /
    • pp.225-231
    • /
    • 2009
  • The objective of this experiment was to study the effects of grass maturity at harvest on the nutritive value of grass silage (GS) in relation to voluntary intake, digestibility, nitrogen (N) utilization and in sacco dry matter (DM) degradability. Silage was cut from a sward dominated by orchardgrass (Dactylis glomerata L.) at the late-vegetative (early-cut), internode elongation (mediumcut) and flowering (late-cut) stages of growth. The DM yield at harvest was the lowest for early-cut silage (5.4 t/ha) and increased to 6.5 and 7.0 t/ha for the medium and late-cut silage respectively. As the crop matured, the crude protein (CP) concentration decreased significantly (p<0.05) and there was a marked increase in acid detergent fiber (ADF) concentration (p<0.001). The three different silages were offered to four 18-month old Charolais wether sheep to measure the voluntary intake, in vivo digestibility and N retention over four 21-day periods in an incomplete changeover design. Silage degradability characteristics were determined using four fistulated sheep to measure DM degradability over 3, 6, 12, 24, 48 and 72 h. There was a linear decrease in the voluntary intake of silage fresh matter, DM, organic matter (OM) and neutral detergent fiber (NDF), digestibility of DM, OM, NDF, ADF and CP, and digestibility of OM in DM (Dvalue) ($P_L<0.01$) as harvesting of grass was delayed. Nitrogen intake, N output in urine, N output in faeces and N balance also linearly decreased ($P_L<0.01$) with postponed harvesting of grass for silage. DM degradability and effective degradability (ED) significantly decreased with increasing maturity of grass at harvest. The results suggest that harvesting date has a significant influence on the nutritive value of GS in terms of intake, digestibility, N balance and in sacco degradability in the rumen. It was concluded that early harvest GS ensured higher intake, digestibility, N intake and DM degradability in comparison with the medium and the late cut GS as a result of improved rumen N efficiency and utilization probably due to a better balance of available energy and protein.

톨 페스큐 신품종 '그린마스터2호'의 품종 특성 및 수량성 (Development and Characterization of Tall fescue Variety 'Greenmaster2ho')

  • 이상훈;김기용;지희정;황태영;박형수;채현석;이기원
    • 한국초지조사료학회지
    • /
    • 제35권1호
    • /
    • pp.26-30
    • /
    • 2015
  • 본 시험은 엔도파이트 프리 톨 페스큐 신품종을 개발하기 위하여 2008년부터 2014년까지 농촌진흥청 국립축산과 학원 초지사료과에서 수행되었다. 톨 페스큐 신품종 '그린마스터2호'는 6배체 품종으로서 잎은 녹색이며, 출수기가 5월 16일경으로서 대비품종인 'Fawn' 보다 5일 늦은 중생품종이고, 출수기에 초장은 82.5 cm 정도이다. 지역적응시험 4개 지역평균 건물수량은 '그린마스터2호'가 15.1톤/ha로서 대비품종인 'Fawn' 보다 29% 많았으며, 지역별로는 4개 지역 모두에서 'Fawn' 보다 생산성이 우수하였다. '그린마 스터2호'의 사료가치는 조단백질(CP) 함량이 13.9%, 건물소화율(IVDMD)이 69.9%, 가소화양분총량(TDN)이 63.8%, 중성세제불용섬유(NDF)가 59.4%, 산성세제불용섬유(ADF) 는 33.5%로서 Fawn과 대등하였다. '그린마스터2호'는 엔도파이트에 감염되지 않는 품종이면서 'Fawn' 보다 국내 기후환경 적응성과 영속성이 우수하여 초지조성에 알맞은 품종으로서 우리나라 전국에서 재배 가능하다.