• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.168-176
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• 2023

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.102-107
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• 1991

This trial was carried out to investigate the effect of oversowing on the fodder value and the life form of standing crop in the low productive grassland with a western exposure at the Korean Native Cattle Improvement Center in Seosan. Chungnam Province. The results obtained are summarized as follows: 1. The inferior plant community of low productive grassland was improved to the most desirable pasture association by oversowing after herbicide application. At the experimental area most of the native grasses were not suitable for the pasture plant due to less fodder value(0-2). 2. The fodder value of standing crop in the improved grassland with 6.04(grasses: 4.72, legumes: 1.07 and herbs: 0.25) was much more increased by 108% than that of low productive grassland. 3. The life forms of plant community in the low productive grassland were composed of Hemicryptophytes of 36.29'0, Therophytes of 30.47~, Chamaephytes of 17.0% and Geophytes of 16.4%). But by oversowing after herbicide application their life forms of Hemicryptophytes increased greatly by 51.4%, on the other hand Therophytes, Chamaephytes and Geophytes were much more decreased by 25.4, 15.6 and 10.470, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.207-211
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• 2015

The objective of the study was to measure the beneficial effects of lactic acid bacteria (LAB) inoculation on the nutritive value of oat silage collected from thirteen regions in the Republic of Korea. The contents of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude ash (CA) were slightly lower in LAB inoculated silage when compared with the control silage, whereas inoculation of LAB resulted in increased total digestible nutrient (TDN). Higher number of LAB, but lower count of yeast and fungi indicated the effectiveness of the LAB inoculation on oat silage fermentation. LAB inoculation resulted in low pH silage, which may prevent undesirable microbial growth. The LAB inoculation promoted lactic acid dominant fermentation with marginal levels of acetic acid and butyric acid in oat silage. These data suggest that the LAB inoculation may preserve oat silage at better quality for ruminant animal production.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.195-200
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• 2015

An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.41-47
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• 1992

Cheju Island has an optimal environment for herviborous animal production. However, the infrastructure has been degraded under the pressure from the development of tourism and thus the technological development for livestock production has not been great. This is the time to re-evaluate and re-direct the system of livestock production on the island. My suggestions on the development of grassland agriculture on the island are as follows : 1. Grassland area of Cheju, 65,695 ha should be maintained and the local government should try to improve more grasslands which can hold 3 or more cattle per ha on pasture. including enterpriseowned lands. 2. For better management of co-operatives village. the local government should evaluate the conditions of the grasslands yearly after being grazed and suggest a new management method accordingly. 3. In order to strengthen grassland research. it is recommended that Cheju Experiment Station be reorganized as a Grassland Research Institute or Department of Grassland Science be added. 4. [mport of good forage varieties. especially legumes, from foreign countries and distribution to farmers after adaption studies, are required to improve nutritive values of forage produced. 5. Methods for production of good quality forage seeds at rcasonablc prices should be established.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.169-173
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• 2011

The performance of grass filter strips (GFS) in abating $PO_4$-P concentrations from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage croplands with rye-corn double cropping system applied with chemical fertilizer and livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated Main plots consisted of the length of GFS, such as 0m, 5m, 10m and 15m. Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). Concentrations of PO4-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10m and 15m reduced $PO_4$-P concentrations significantly compared to that with 0m and 5m (p<0.05). The results from this study suggest that GFS improved the removal and trapping $PO_4$-P from forage croplands.

• Asian Journal of Turfgrass Science
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• v.11 no.4
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• pp.303-309
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• 1997

The investigation was cycle of aluminum of surface soil elements in dynamic grassland ecosystems at a steady state in a Zoysia japonica and a Miscanthus sinensis ecosystem in Mt. Kwanak, Korea. Average amounts of total storage for aluminum in Z japonica and M. sinensis grasslands were 8,426mg /$m^2$ and 7,849mg /$m^2$ respectively. Decay constants estimated on the base of experimental and mathematical model, were 0.04 in Z japonica grassland, and 0.08 in M. sinensis grassland. Half time to decay aluminum of litter soils were 17.33 years in Z japonica grass-land, and 8.66 years in Al. sinensis grassland. 95% decay times in Z japonica, and in M. sinensis grassland were 75.0 years and 35.0 years respectively. Needed times to lose almost all of elements in Z japonica and M. sinensis grassland were 125.0 years, and 62.50 years respectively. The metals were losed more rapidly in M. sinensis than in Z japonica grassland. The cycle of aluminum was investigated to be related with soil acidity. Key words: Cycle of aluminum, Zoysia japonica. Miscanthus sinensis, Mt. Kwanak, Decay constants, Soil acidity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.208-214
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• 2022

Drought stress is a condition that occurs frequently in the field, it reduces of the agricultural yield of field crops. The aim of the study was to screen drought-adapted genotype of Italian rye grass. The experiments were conducted between the two Italian ryegrass (Lolium multiflorum L.) cultivars viz. Hwasan (H) and Kowinearly (KE). The plants were exposed to drought for 14 days. The results suggest that the morphological traits and biomass yield of KE significantly affected by drought stress-induced oxidative stress as the hydrogen peroxide (H₂O₂) level was induced, while these parameters were unchanged or less affected in H. Furthermore, the cultivar H showed better adaptation by maintaining several physiological parameter including photosystem-II (Fv/Fm), water use efficiency (WUE) and relative water content (RWC%) level in response to drought stress. These results indicate that the cultivar H shows improved drought tolerance by generic variation, improving photosynthetic efficiency and reducing oxidative stress damages under drought stress. These findings can be useful to the breeder and farmer for improving drought tolerance in Italian rye grass through breeding programs.

• Animal Bioscience
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• v.35 no.6
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• pp.838-846
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• 2022

Objective: Native plants can be used as additives to replace antibiotics to improve ruminant feed utilization and animal health. An experiment was conducted to evaluate the effects of Gentiana straminea (GS) on nutrient digestibility, methane emissions, and energy metabolism of Simmental calves. Methods: Thirty-two (5-week-old) male Simmental clves, with initial body weight (BW) of 155±12 kg were fed the same basal diet of concentrates (26%), alfalfa hay (37%), and oat hay (37%) and were randomly separated into four treatment groups according to the amount of GS that was added to their basal diet. The four different groups received different amounts of GS as a supplement to their basal diet during whole experiment: (0 GS) 0 mg/kg BW, the control; (100 GS) 100 mg/kg BW; (200 GS) 200 mg/kg BW; and (300 GS) 300 mg/kg BW. Results: For calves in the 200 GS and 300 GS treatment groups, there was a significant increase in dry matter (DM) intake (p<0.01), average daily gain (ADG) (p<0.05), organic matter intake (p<0.05), DM digestibility (p<0.05), neutral detergent fibre (NDF) digestibility (p<0.05), and acid detergent fibre (ADF) digestibility (p<0.05). Dietary GS supplementation result in quadratic increases of DM intake (p<0.01), ADG (p<0.05), NDF intake (p<0.05), and ADF intake (p<0.05). Supplementing the basal diet with GS significantly increased nitrogen (N) retention (p<0.001) and the ratio of retention N to N intake (p<0.001). Supplementing the basal diet with GS significantly decreased methane (CH₄) emissions (p<0.01), CH₄/BW^0.75 (p<0.05) and CH₄ energy (CH₄-E) (p<0.05). Dietary GS supplementation result in quadratic increases of CH₄ (p<0.01) and CH₄/DM intake (p<0.01). Compared with 0 GS, GS-supplemented diets significantly improved their gross energy intake (p<0.05). The metabolizable energy and digestive energy intake were significantly greater for calves in the 100 GS and 200 GS calves than for 0 GS calves (p<0.05). Conclusion: From this study, we conclude that supplementing calf diets with GS could improve utilization of feed, energy, and N, and may reduce CH₄ emissions without having any negative effects on animal health.

• Journal of Plant Biology
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• v.21 no.1_4
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• pp.7-12
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• 1978

The results of the relationship between standing crop of Miscanthus sinensis grassland and soil morphological characteristics are as follows. The Miscanthus sinensis grassland seems to grow well in volcainc ash soil. The depth of A layer was closely related to the standing crop of the Miscanthus sinensis grassland. The root systems of Miscanthus sinensis reached to its maximum in A layer. The root systems of Miscanthus sinensis showed its maximum at 20mm and below(soil hardness). The soil texture of A layer showed SL-SiL. The soil structure of A layer contained Massive-Small Granular. The soil colors of A layer expressed Dark Yellowish Orange-Brownish Black.