• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1286-1289
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• 2008

The ability of Lactococcus strains to inhibit the growth of intestinal bacteria was examined. In in vitro cocultures, we observed that among eighteen Lactococcus strains tested, the ability to inhibit growth of Escherichia coli varied, with the L. lactis N7 showing the greatest growth inhibition. Strain N7 ($8.94\times10^{10}$ CFU/day for 7 days) was orally administered to mice, and the viable count of strain N7 in feces appeared at a level of $10^{4-5}$ CFU/g. After administration, the proportion of Bacteroidaceae to total intestinal bacteria decreased. Lactococci may act as probiotic bacteria by inhibiting the growth of harmful bacteria.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1564-1567
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• 2002

Influence of dietary sesame meal on plasma glucose, non-esterified fatty acid (NEFA), triglyceride, total cholesterol, high-density lipoprotein (HDL)-cholesterol and urea concentrations in goats was examined. Goats were fed a control diet (50% timothy hay and 50% concentrates) (CD) or a sesame meal diet (50% timothy hay, 25% concentrates and 25% sesame meal) (SMD) during 12 days. Blood samples were taken after overnight fasting and afternoon every day. Body weight was not changed by feeding either CD or SMD. The concentrations of plasma triglyceride and urea were higher (p<0.05) in goats fed SMD than those fed CD. Plasma NEFA concentration was higher in plasma samples after overnight fasting. Plasma glucose concentration in plasma samples collected afternoon was higher than those after overnight fasting. Plasma total cholesterol concentration was significantly increased by feeding SMD but not by feeding CD, which was due to the remarkable increase of plasma HDL-cholesterol concentration. In conclusion, dietary sesame meal brought about an increase in plasma total cholesterol concentration accompanied with an increment in plasma HDL-cholesterol consentration in goats.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.285-285
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• 2017

This study is aimed to analyze the effect of lactic acid bacteria inoculants (LAB) on quality of rye silage fermentation, harvested at early heading stage. The nutritive values were similar between the control and LAB inoculated silages. The pH of rye silage in LAB inoculation significantly decreased as compared to control (p<0.05). In addition, the content of lactic acid in LAB inoculation significantly increased (p<0.05), but the content of acetic acid in LAB treatments decreased. In addition, lactic acid bacterial counts in LAB inoculation significantly increased as compared to control (p<0.05). Therefore, we suggest that rye silage could be improved by novel lactic acid inoculation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.187-192
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• 2014

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.1 no.1
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• pp.2-9
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• 1978

A review of factors influencing grass and clover establishment, survival and yield at oversowing was made from the experimental results of home and abroad. The following conclusions are considered: (1) The existing vegetation present at oversowing appeared to be the most critical factor reducing establishment and survival of grass. Therefore, it is essential to check competition from the native vegetation before oversowing. (2) Although lime had comparatively little effect on yield of grassland, the general effect of lime should be more emphasized under our acid soil conditions to promote the availability of all the essential elements and the growth of microorganisms, and to reduce the toxic effects of nutrients. One to two tons of lime per ha at oversowing would be useful. (3) Phosphorus is one of the nutrients most generally deficient in grassland soils of Korea, consequently, this nutrients applied at oversowing is very effective. Application as much as 200kg of phosphorus per ha would be essential. (4) The effect of nitrogen on the establishment and survival of grass depends on the amount and density of the herbage present. The use of nitrogen in dense herbage adversely affected grass establishment and survival, possible because the fertilizer stimulated the growth of the eisting herbage. Around 40kg of nitrogen per ha may be enough at oversowing (5) Potassium is not as universally deficient in soils of native grassland as phosphorus. Therefore, it cannot be over-emphasized at oversowing. Studies determinig the optimum amount of potassium at of oersowing are needed.

• Korean Journal of Agricultural Science
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• v.13 no.2
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• pp.219-226
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• 1986

A study was conducted to provide direct comparisons of the effect of variations in herbage use, grazing and social behaviour upon the flat, slope and forest grassland with a total 30 Korean Native Cattle and 5 Korean Native Goats under 4-year old grassland established by intensive sowing method in Daecheon. The results obtained are summarized as follows; 1. Relative intake index, dry matter intake per animal and dry matter digestibility by Korean Native Goats in flat, slope and forest grassland were 35.2%-462g-63.7%, 35.0%-459g-63.0% and 29.8%-391g-62.1%, respectively. 2. Grazing time by Korean Native Cattle was not different among the grassland types, but ruminating time was increased in slope grassland, whereas in forest grassland was decreased. Resting time was increased in forest grassland, whereas in slope grassland was decreased. Walking time was increased in flat grassland, but loafing time was increased in forest grassland. The number of rumination, chews per bolus and defecation number were decreased in forest grassland. The number of drinks, total drinking water and walking distance were increased in slope grassland. 3. Animal distance, occupied area per animal and sub group formation by Korean Native Cattle in flat, slope and forest grassland were $3.4m-11.9m^2-3.6head$, $3.56m-11.0m^2-3.7head$ and $3.70m-14.6m^2-3.4head$, respectively. The order of grazing movement was similar to the pear-shaped grazing formation, but the relations of dominance between first grazer and last grazer upon different grassland types was not clear.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.4
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• pp.268-273
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• 1993

With a purpose of finding out the effects of phosphatic fertilizer application at the grassland establishment and management on the botanical composition, mineral content of grasses and the chemical properties of soil. a field experiment was arranged with four treatments as a randomized complete block design and lasted from September, 1989 to October, 1991 in hilly area near Suweon. The results obtained are summarized as follows; 1. The botanical composition of grassland that phosphatic fertilizer was not applied was poor and the rate of legumes was increased with phosphatic fertilizer application and the effect of phosphatic fertilizer applications was obvious at the grassland management. 2. The mineral contents, N. P, Ca, Mg of grasses were increased with phosphatic fertilizer application but the K/Ca+Mg equivalent ratio of grasses was lowered and K content and Ca/P ratio tended not to be regular. 3. The available phosphorus contents and exchangable cation, Ca, Mg, contents of grassland soil were increased with phosphatic fertilizer applications but potasium contents tended not to be regular. 4. It was necessary to apply phosphatic fertilizer for the establishment and management of grassland. The botanical composition of grassland that phosphatic fertilizer was not applied at the establishment could be corrected into good stand with continuous phosphatic fertilizer applications at the management.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.2
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• pp.105-112
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• 2006

This experiment was carried out to investigate the distribution, function of dry matter and competition in the grassland composed with mainly perennial ryegrass. Co-functions between grasses and weeds were evaluated by calculating methods; expected yield (P), agressivity, relative yield total (RYT), compensation index (CI) and morphological index (MI). Each values obtained were influenced by seeding method, grassland management and seasonal changes of co-functional index were identical. Expected yields of spring were lower than those of fall and improved by additional seeding to grassland in early spring time. In the relative yield total (RYT), all were under 1.0 which ranged from 0.17 to 0.41. Compensation index (CI) ranged from -0.3 to -0.6 and all values showed 'under compensation(+, --)' This means that the conditions of 'under compensation' could be improved through weed control by management of grassland in perennial ryegrass grassland. The results indicated that productivity of grassland mainly composed with perennial ryegrass is negatively affected by weeds like shepherd's purse, crabgrass and barnyard grass. As major weeds, shepherd's purse in spring, crabgrass in summer and barnyard grass in summer and fall were negatively functioned to productivity in the grassland composed with mainly perennial ryegrass.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.2
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• pp.225-231
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• 2009

The objective of this experiment was to study the effects of grass maturity at harvest on the nutritive value of grass silage (GS) in relation to voluntary intake, digestibility, nitrogen (N) utilization and in sacco dry matter (DM) degradability. Silage was cut from a sward dominated by orchardgrass (Dactylis glomerata L.) at the late-vegetative (early-cut), internode elongation (mediumcut) and flowering (late-cut) stages of growth. The DM yield at harvest was the lowest for early-cut silage (5.4 t/ha) and increased to 6.5 and 7.0 t/ha for the medium and late-cut silage respectively. As the crop matured, the crude protein (CP) concentration decreased significantly (p<0.05) and there was a marked increase in acid detergent fiber (ADF) concentration (p<0.001). The three different silages were offered to four 18-month old Charolais wether sheep to measure the voluntary intake, in vivo digestibility and N retention over four 21-day periods in an incomplete changeover design. Silage degradability characteristics were determined using four fistulated sheep to measure DM degradability over 3, 6, 12, 24, 48 and 72 h. There was a linear decrease in the voluntary intake of silage fresh matter, DM, organic matter (OM) and neutral detergent fiber (NDF), digestibility of DM, OM, NDF, ADF and CP, and digestibility of OM in DM (Dvalue) ($P_L<0.01$) as harvesting of grass was delayed. Nitrogen intake, N output in urine, N output in faeces and N balance also linearly decreased ($P_L<0.01$) with postponed harvesting of grass for silage. DM degradability and effective degradability (ED) significantly decreased with increasing maturity of grass at harvest. The results suggest that harvesting date has a significant influence on the nutritive value of GS in terms of intake, digestibility, N balance and in sacco degradability in the rumen. It was concluded that early harvest GS ensured higher intake, digestibility, N intake and DM degradability in comparison with the medium and the late cut GS as a result of improved rumen N efficiency and utilization probably due to a better balance of available energy and protein.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.26-30
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• 2015

A new tall fescue variety (Festuca arundinacea Schreb.) named 'Greenmaster2ho' was developed by the National Institute of Animal Science, Rural Development Administration, in Cheonan, Korea from 2010 to 2014. For the synthetic seed production of this new variety, 5 superior clones were selected and polycrossed: 09XFa02, 09XFa03, 09XFa11, 09XFa13, and 09XFa14. The agronomic growth characteristics and forage production capability of the seeds were studied at Cheonan from 2010 onward, and regional trials were conducted in Cheonan, Hoengseong, Jeju, and Jinju from 2012 to 2014. Greenmaster2ho showed resistance to disease, persistence, and regrowth ability that were all enhanced when compared with Fawn. At 15,119 kg/ha, the dry matter (DM) yield of Greenmaster2ho was 29% higher than that of Fawn, but the nutritive value of both varieties as forage was similar. This study aimed to make a contribution to the vitalization of the Korean grassland industry by developing a new tall fescue variety with excellent environmental adaptability.