• 대한한의학방제학회지
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• 제21권1호
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• pp.177-185
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• 2013

Objectives : Leejung-tang (Lizhong-tang) has been used for treatment of gastrointestinal disorders in Korea. In this study, we performed quantification analysis of five marker components, liquiritin, ginsenoside Rb1, ginsenoside Rg1, glycyrrhizin, and 6-gingerol in Leejung-tang using a ultra performance liquid chromatography- electrospray ionization-mass spectrometer (UPLC-ESI-MS). In addition, we evaluated antioxidant activity of Leejung- tang. Methods : The column for separation of five constituents used a UPLC BEH C18 ($100{\times}2.1mm$, $1.7{\mu}m$) maintained at $45^{\circ}C$. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in H2O (A) and CH3CN (B) by gradient flow. The flow rate was 0.3 mL/min with detection at mass spectrometer. The antioxidative activities conduct an experiment on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of Leejung-tang. Results : Calibration curves of five marker compounds were acquired with r2 values > 0.99. The amount of the five compounds in Leejung-tang were 0.07 - 0.84 mg/g. The concentration required for 50% reduction (RC50) against ABTS radical was 119.02 ug/mL. In addition, the scavenging against DPPH radical of Leejung-tang was 11.4%, 14.5%, 19.8%, 29.6%, and 49.2% at 25 ug/mL, $50{\mu}g/mL$, $100{\mu}g/mL$, $200{\mu}g/mL$, and $400{\mu}g/mL$, respectively. Conclusions : The established LC-MS/MS method will be helpful to improve quality control of Leejung-tang. In addition, Leejung-tang is a potential antioxidant therapeutic agent.

• 대한한의학회지
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• 제32권3호
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• pp.25-34
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• 2011

Objectives: We performed simultaneous determination of five constituents by HPLC in Samul-tang (SMT). Additionally, we investigated the immune-stimulatory potential of SMT on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. The extract of SMT (1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Results: Calibration curves were acquired with $r^2$>0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 mg/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). Also, SMT significantly enhanced OVAspecific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group. Conclusions: The established method will be applied for the quantification of major components and immunestimulating activity in OVA-immunized mouse model of SMT.

• 디지털콘텐츠학회 논문지
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• 제10권4호
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• pp.579-585
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• 2009

Tyrtshnikov[9]의 연구에서는 토플리츠 선형시스템에서 토플리츠 선행조건으로 일반해를 구하는 방법들을 제시하고 있다. 또한 대칭 토플리츠 행렬에서의 선행조건 행렬을 선택하는 방법도 소개 하였다. 본 연구는 토플리츠 시스템에서 새롭게 선행조건 찾는 방법을 소개하고 있으며, 선행조건행렬들의 분석을 통해 대칭 토플리츠 행렬의 고유값들과 대칭 토플리츠행렬로 부터 생성된 선행조건행렬의 고유값들이 매우 근접하다는 결과를 나타내고 있다. 즉, 선행조건시스템 $C_0^{-1}T$의 고유값들은 1에 모두 접근하게되면, 선행조건 시스템의 수렴속도는 superlinear이다. 본 연구에서 생성된 선행조건행렬 $C_0$은 선행조건시스템의 superlinear의 수렴속도로 계산하게 된다. 또한 토플리츠 행렬은 이미지 프로세싱이나 시그널 프로세싱에서 많이 응용되고 있으므로 본 연구에서 개발한 선행조건행렬로부터 다양한 응용성을 높일 수 있다. 본연구의 또 다른 특징은 토플리츠 행렬의 중요한 성질을 보존하면서 선행조건행렬을 생성하였다.

• Animal cells and systems
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• 제2권4호
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• pp.495-500
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• 1998

D-type G1 cyclins are known to be crucial for the progression of mitotic cell cycle in mammals. Although many studies have been performed to elucidate the roles of D-type cyclins, it is largely unknown whether D-type cyclins are directly involved in the regulation of meiotic germ cell development. In the present study, we examined the expression patterns of D-type cyclins (cyclin D1 and D3) during male germ cell development by northern blot and in situ Hybridization analyses. In the adult testes, we detected a 4.2 kb cyclin D1 mRNA and two different sizes (2.3 kb and 1.8 kb) of cyclinD3 mRNAs. The short form of the cyclin D3 transcript was testis-specific. Along with the testicular development, expression of cyclin D3 mRNA was increased whereas cyclin D1 mRNA was gradually decreased. in situ hybridization study also revealed that the expression of cyclin D3 was restricted to the postmeiotic germ cells. Furthermore, the 2.3 kb transcript was highly expressed in the round spermatids and decreased in the elongated spermatids/residual bodies, while the 1.8 kb transcript was expressed in elongated spermatids/residual bodies more abundantly. Sucrose-gradient separation of polysomal RNA fractions demonstrated that some portions of the 2.3 kb transcript are translationally active, while the 1.8 kb transcript is likely to be inactive. Taken together, the present data suggest a functional importance of cyclin D3 expression in the differentiated postmeiotic male germ cells.

• 한국컴퓨터정보학회논문지
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• 제19권12호
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• pp.187-196
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• 2014

본 논문에서는 고정된 역간 거리를 정해진 운전 시분내에 주행에너지를 최소화하며 주행하는 열차의 특성을 파악하고 수학적으로 모델링한다. 도시철도차량 자동주행에 일반적으로 사용되는 PID제어기 대신 목표값에 추종하면서도 자동 주행 중 소비에너지가 최소화되도록 최적제어기를 사용하여 철도 차량를 모델링하였으며 실제 동일한 운행조건하에서 설계한다. 실제 선로 조건을 적용하여 별도의 차상장치나 선로주변시설 없이도 자동운전 중 주행에너지를 최소하여 주행에너지를 절감하고자 한다. 따라서 8호선 실 노선 구간별 운전시분 내에서 실측 데이터 분석을 위해 직선구간/구배구간/곡선구간 등 구간을 선정하고 그 구간에서 열차의 운행패턴에 따라 에너지를 절감하는 열차운행을 방법을 제시하였다.

• 말소리와 음성과학
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• 제6권4호
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• pp.53-65
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• 2014

The current research examines whether pitch contour shapes in North Kyungsang pitch accent contrasts provide a phonetic dimension for phonological discreteness in a mimicry task. Two pitch accent continua resynthesized were created for HH vs. HL and HH vs. LH. To confirm a phonetic dimension for accounting for pitch accent categories in North Kyungsang Korean, the mimicries of speakers of two dialects (i.e., North Kyungsang & South Cholla) were compared. One of the findings showed that, for North Kyungsang speakers, the range of mean f0 peak times was a phonetic dimension undergoing a continuous shift within a stimulus continuum for both HH vs. HL and HH vs. LH. On the other hand, for South Cholla speakers, there were no apparent shifts around categorical boundaries for either HH vs. HL or HH vs. LH. Regarding individual mimicries on f0 peak timing, there are many variations. For HH vs. LH, three North Kyungsang speakers showed a discrete pattern reflecting a shift in phonological categories, but for HH vs. HL, there was no such distinction showing a categorical shift, though there were statistically significant differences for two speakers. Interestingly, one of the North Kyungsang speakers showed a continuous phonetic dimension for both HH vs. HL and HH vs. LH. Lastly, the f0 valley timing did not exhibit a discrete or gradient phonetic dimension for speakers of either dialect. On the basis of these results, what is interesting is that the tonal target such as high tone in North Kyungsang pitch accent categories within the autosegmental-metrical (AM) theory may be realized within individual cognitive systems for representing the interaction of perception and production.

• 대한전자공학회논문지SP
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• 제47권2호
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• pp.77-85
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• 2010

온라인 지문 인식 시스템에서는 주변 환경, 사용자의 지문 상태 및 입력 방법에 따라 다양한 품질의 지문이 입력된다. 따라서 지문 인식 시스템의 성능을 향상시키기 위해서는, 입력된 지문 영상을 이용하여 본인과 타인간의 변별력을 높이는 연구뿐만 아니라 다양한 품질의 지문 영상들 중에서 품질이 좋은 지문 영상을 선택하여 이를 인식에 사용하는 연구도 병행이 되어야 한다. 하지만 대부분의 기존 연구에서는 지문의 지역적인 품질만을 측정하였기 때문에 한 장의 지문영상의 품질에 대한 예측은 거의 이루어지지 않았다. 따라서 본 논문에서는 획득된 지문 영상의 품질을 판단하기 위해서 지역적인 지문 품질 측정과 이를 통한 전역적 지문 품질 측정 방볍을 제안하였다. 지역적인 지문 품질 평가에서는 각 지문 블록에서 그레디언트(Gradient)의 확률 밀도 함수(Probability Density Function)의 형태를 측정하여 블록 별 품질 값을 예측하였고, 이를 기반으로 전역적인 품질 평가에서는 신경망(Neural network)올 사용하여 지문 영상 전체를 평가함으로써 입력된 영상의 사용 여부를 판단하였다. FVC2002 데이터베이스를 사용하여 실험한 결과, 제안한 전역적 방법을 사용하였을 때 NFIQ(NIST Fingerprint Image Quality)의 방법보다 정합 예측 성능이 높게 나타난 것올 확인할 수 있었다.

• Structural Engineering and Mechanics
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• 제64권3호
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• pp.361-379
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• 2017

In this article, the vibration behavior of double-bonded sandwich microplates with homogeneous core and nanocomposite facesheets reinforced by carbon nanotube and boron nitride nanotube under multi physical fields such as 2D magnetic and electric fields is investigated. Symmetric and un-symmetric distributions of nanotubes are considered for facesheets of sandwich microplates such as uniform distribution and various functionally graded distributions. The double-bonded sandwich microplates rest on visco-Pasternak foundation. Material properties of sandwich microplates are obtained by the extended rule of mixture. The sinusoidal shear deformation theory (SSDT) is employed to describe displacement fields of sandwich microplates. Also, the dimensionless natural frequency is obtained by classical plate theory (CPT) and compared with the obtained results by SSDT. It can be seen that the obtained dimensionless natural frequencies by CPT are higher than SSDT. In order to study the material length scale parameters, modified strain gradient theory at micro scale is utilized and then, the equations of motion are derived using Hamilton's principle. The effects of different parameters such as foundation parameters including Winkler, shear layer and damping coefficients, various distributions and volume fraction of nanotubes, core to facesheet thickness ratio, aspect and side ratios on the dimensionless natural frequencies are discussed in details. The results of present work can be used to optimum design and control of similar systems such as micro-electro-mechanical and nano-electro-mechanical devices.

• 대한한의학방제학회지
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• 제20권2호
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• pp.37-46
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• 2012

Objectives : Oyaksungi-san(Wuyaoshunqisan) has been used for treatment of stroke and rheumatoid arthritis in Korea. In this study, a simple and accurate high-performance liquid chromatography(HPLC) method was established for simultaneous determination of six main components, liquiritin, ferulic acid, naringin, hesperidin, neohesperidin, and glycyrrhizin in Oyaksungi-san, a traditional Korean herbal prescription. Methods : The analytical column for separation of six constituents was used a Gemini $C_{18}$ column maintained at $40^{\circ}C$. The mobile phase consisted of two solvent systems, 1.0% (v/v) acetic acid in $H_2O$ (A) and 1.0% (v/v) acetic acid in acetonitrile (B) by gradient flow. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 254 nm for glycyrrhizin, 280 nm for liquiritin, naringin, hesperidin, and neohesperidin, and 320 nm for ferulic acid. Results : Calibration curves were acquired with $r^2$ values ${\geq}0.9998$. The results of recovery test were 91.58%-105.90% with a relative standard deviations (RSDs, %) value less than 2.0%. The values of RSD for intra- and inter-day precision were 0.03%-1.72% and 0.03%-1.63%, respectively. The contents of the six compounds in Oyaksungi-san were 0.33-9.30 mg/g. Conclusions : The newly established HPLC method will be helpful to improve quality control of Oyaksungi-san.

• Journal of Korean Neurosurgical Society
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• 제29권12호
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• pp.1634-1641
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• 2000

Objective : The cerebrospinal fluid(CSF) pulsates within the craniospinal axis in response to rhythmic cerebral blood volume variation during the cardiac cycle. The aim of this study is to characterize the normal and abnormal CSF flow and its waveforms in the cervical spinal subarachnoid space. Methods : The magnetic resonance(MR) images were obtained with 1.5 T(GE Signa, GE Medical Systems, Milwaukee, USA) unit using the 2 dimensional cine PC(phase contrast) sequence with cardiac gating and gradient recalled echo imaging. This pulse sequence yielded 16 quantitative flow-encoded images per cardiac cycle. Sagittal and axial images of the cervical spinal CSF space were obtained, and target sites were analyzed for characteristic CSF flow (TR=50ms, TE=12.5-15ms). The region of interest(ROI) was 1mm 3 in volume. Twenty six persons were included in this study : 10 healthy volunteers and 16 patients with cervical myelopathy. The post-operative cine MR study were also done in five patients. Results : The normal CSF pulsation dynamics in the cervical spine showed discrete systolic and diastolic components. The CSF flow revealed a sine wave pattern, in which the systolic phase was shorter than the diastolic phase(ratio=2 : 3). The patient group revealed decreased amplitudes of the CSF flow and irregularly distored flow waves. The systolic phase was elongated in the ROI above the stenotic level, whereas the diastolic phase was lengthened below the level. In the postoperative images, the abnormal pattern and amplitude were found to be corrected. Conclusion : From these results, the authors believe that the CSF flow study provides valuable informations regarding the extent of cervical stenosis and may be useful for the surgical planning and post-operative evaluation.