This study aimed to provide basic data for the development of education programs on food hygiene attitude and personal hygiene management of high school students according to gender. From July 16 to October 15, 2015, questionnaires were distributed to 30 students per grade at 16 high schools in Chungcheongnam-do, and a total of 1,214 questionnaires(excluding uncollected and insufficient questionnaires) were processed using SPSS(ver 18.0). According to the study, female students more checked the expiration date for food(3.78, 3.57), ate stored food without heating(2.83, 3.02) and stored it according to the characteristics(3.92, 3.72) than male students. On the other hand, male students answered they looked at food packaging more closely(3.34, 3.22), and always used personal plates when they ate(3.43, 3.32), than female students. Female students were always better at hand washing after went to toilet(4.25, 3.94), used individual cups when using water purifiers(3.45, 3.32), kept nails cleaner(3.61, 3.49), and went around(2.14, 2.53) and went to toilet during meals than male students(2.22, 2.57). Boys answered they were better at hand washing before meals(3.64, 3.50), with right way(3.58, 3.46), and covering with a tissue, handkerchief or sleeve when they coughed(3.55, 3.37) than girls. In this study, high school students showed significant differences between gender in food hygiene attitude and personal hygiene management, with different areas requiring improvement. Therefore, this study suggests the need for education on food hygiene attitude and personal hygiene management differentiated according to gender.