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The Effects of Ozone Gas on Wound Healing in Skin (오존 가스가 피부창상의 치유에 미치는 영향)

  • Lee, Soo-jin;Lee, Jae-il;Kim, Myung-cheol;Hong, Sung-hyuk;Kim, Duck-hwan;Cho, Sung-whan
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.493-500
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    • 2003
  • This study was performed to determine the optimal dose of ozone for wound healing. Twenty Korean black goats were allocated to 4 groups. The skin wound with $1{\times}1cm$ was induced over the hoof. For 5 minutes per day, 0.1 ppm (Group I), 1 ppm (Group II) and 6 ppm (Group III) ozone gas was applied. In control group, 5 ml of normal saline was applied daily on the wound. Wound square was determined using color image analyzer at day 0, 4 and 14. Soft tissue depth of wound was determined using radiograph. Before the application of ozone gas, $1{\times}1cm$ of skin tissue was resected and observed for histopathologic findings. To determine the healing effect, $2{\times}1cm$ of wound tissue was resected and observed for histopathologic findings at day 14. In mean squares of wound, all experimental groups revealed more reduced mean squares than control group at day 4, and especially Group II (p<0.05) and Group III (p<0.05) revealed significant reduction. At day 14, Group I and Group II (p<0.05) revealed more reduced mean squares than control group, but Group III revealed less reduced mean squares than control group. In soft tissue depth of wound, all experimental groups and control group revealed swelling at day 4, compared with day 0. At day 14, all experimental groups and control group revealed reduction, compared with day 4, and especially Group II (p<0.05) revealed significant reduction. In histopathological findings, inflammation findings in epidermis and dermis, hemorrhagic finding in epidermis were revealed in control group. In Group I, inflammation finding was reduced, compared with control group. Formation of scab and many reepithelialization of epidermis were revealed. In Group II, inflammation findings in epidermis and dermis which were observed in control group were not found, normal skin tissue was revealed. In Group III, inflammation findings in epidermis and dermis were revealed, reepithelialization of epidermis was not founded. Slow healing process was observed, compared with Group I and II. It was concluded that Group II has excellent promotion effect of wound healing.

Comparison of Diluents on Liquid Storage of Korean Native Goat Spermatozoa (희석액의 종류가 재래 흑염소 액상 정액의 생존율에 미치는 영향)

  • Kim, H.J.;Choe, J.Y.;Choi, S.H.;Son, D.S.;Choi, S.H.;Sang, B.D.;Han, M.H.;Ryu, I.S.;Kim, I.C.;Kim, I.H.;Im, K.S.;Kim, S.J.;Cho, S.R.
    • Journal of Embryo Transfer
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    • v.21 no.4
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    • pp.339-344
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    • 2006
  • This study was to investigate the optimal short-term storage diluents for goat spermatozoa. Semen was collected with electro-ejaculation from two goats. The collected semen was diluted in BTS and centrifuged at 500 g for 5 minutes. The supernatant was discarded and diluted with BTS, Modena or Triladyl$^{(R)}$ and stored at 4 or $17^{\circ}C$ for 8 days. The motility of spermatozoa in BTS and Modena stored at $4^{\circ}C$ was rapidly decreased at day 1. The motility of spermatozoa in BTS at $17^{\circ}C$ was decreased to 30$\sim$45% at day 2. In Modena at $17^{\circ}C$, the inappropriate motility fur artificial insemination (AI) was reached at day 4. The spermatozoa stored in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ were more viable and higher motility up to day 4. In conclusion, liquid storage of goat spermatozoa in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ for 4 days showed permissible viability for AI.

Accuracy of Ultrasonography in Early Pregnancy Diagnosis in Doe

  • Singh, N.S.;Gawande, P.G.;Mishra, O.P.;Nema, R.K.;Mishra, U.K.;Singh, Mohan
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.760-768
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    • 2004
  • The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.

Different Sources and Levels of Copper Supplementation on Performance and Nutrient Utilization of Castrated Black Bengal (Capra hircus) Kids Diet

  • Mondal, M.K.;Biswas, P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.7
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    • pp.1067-1075
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    • 2007
  • Twenty eight 3-4 month old castrated Black Bengal kids (Capra hircus) were used to determine the effects of source and level of dietary copper (Cu) concentration on their performance and nutrient utilization. Cu was supplemented (0, 10, 20 and 30 mg/kg diet DM) as copper sulfate ($CuSO_4$, $5H_2O$) or copper proteinate (Cu-P). Kids were fed a basal diet containing maize (19.5%), soybean (17.0%), deoiled rice bran (56.5%), molasses (4.0%), di-calcium phosphate and salt (1.0% each) and mineral and vitamin mixture (0.5% each) supplements at 3.5% of body weight to meet NRC (1981) requirements for protein, energy, macro minerals and micro minerals, excluding Cu. The basal diet contained 5.7 mg Cu/kg, 122.5 mg Fe/kg, 110 mg Zn/kg, 0.26 mg Mo/kg and 0.32% S. $CuSO_4$ or Cu-P was added to the basal diet at the rate of 10, 20 and 30 mg/kg. Kids were housed in a well ventilated shed with facilities for individual feeding in aluminum plated metabolic cages. Blood samples were collected from the jugular vein on d 0, 30, 60 and 90 to determine hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC) and serum enzymes (alkaline phosphatase, alanine transferase and aspertate transferase). A metabolism trial of 6 days duration was conducted after 90 days of experimental feeding. Statistical analysis revealed that source and level of Cu supplementation improved live weight gain (p<0.04) and average daily gain (p<0.01). No significant contribution of source and level of Cu to alter serum serum enzymes was evident. Goats fed Cu-P tended to have higher Hb, PCV and TEC than with $CuSO_4$ supplementation. Cu-P increased digestibility of ether extract (EE, p<0.02) and crude fiber (p<0.05) and showed an increasing trend (p<0.09) for digested crude protein (CP) and crude fiber (CF). Supplemental dose of Cu linearly improved (p<0.02) digestibilities of dry matter (DM), organic matter (OM), EE and nitrogen free extract (NFE). Though the absorption of nitrogen (N) was not affected (p>0.10) by both source and dose of Cu, N retention was affected (p<0.04) and there was a significant $Source{\times}Dose$ interaction (p<0.05). Final body weight (BW) was not influenced (p>0.10) by the source of Cu but increasing dose of Cu increased (p<0.04) the BW of kids. TDN intake (g/kg $W^{0.75}$) was higher (p<0.05) with the increased dose of Cu and there was a significant $Source{\times}Dose$ interaction. It was concluded that supplementation of Cu from different sources and varying dose level in a concentrate based diet may improve performance, nutrient utilization and plane of nutrition in castrated Black Bengal kids. The effects on performance and nutrient utilization are more pronounced with Cu-P than $CuSO_4$ supplementation. Higher dose of Cu showed better result than lower dose.

Effect of Age on Energy Requirement for Maintenance and Growth of Dorper and Hu Crossbred F1 Ewes Weighing 20 to 50 kg

  • Nie, H.T.;Wan, Y.J.;You, J.H.;Wang, Z.Y.;Lan, S.;Fan, Y.X.;Wang, F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1140-1149
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    • 2015
  • This research aimed to define the energy requirement of Dorper and Hu Hybrid $F_1$ ewes 20 to 50 kg of body weight, furthermore to study energy requirement changes with age and evaluate the effect of age on energy requirement parameters. In comparative slaughter trial, thirty animals were divided into three dry matter intake treatments (ad libitum, n = 18; low restricted, n = 6; high restricted, n = 6), and were all slaughtered as baseline, intermediate, and final slaughter groups, to calculate body chemical components and energy retained. In digestibility trial, twelve ewes were housed in individual metabolic cages and randomly assigned to three feeding treatments in accordance with the design of a comparative slaughter trial, to evaluate dietary energetic values at different feed intake levels. The combined data indicated that, with increasing age, the net energy requirement for maintenance ($NE_m$) decreased from $260.62{\pm}13.21$ to $250.61{\pm}11.79kJ/kg^{0.75}$ of shrunk body weight (SBW)/d, and metabolizable energy requirement for maintenance (MEm) decreased from $401.99{\pm}20.31$ to $371.23{\pm}17.47kJ/kg^{0.75}$ of SBW/d. Partial efficiency of ME utilization for maintenance ($k_m$, 0.65 vs 0.68) and growth ($k_g$, 0.42 vs 0.41) did not differ (p>0.05) due to age; At the similar condition of average daily gain, net energy requirements for growth ($NE_g$) and metabolizable energy requirements for growth ($ME_g$) for ewes during late fattening period were 23% and 25% greater than corresponding values of ewes during early fattening period. In conclusion, the effect of age upon energy requirement parameters in the present study were similar in tendency with previous recommendations, values of energy requirement for growth ($NE_g$ and $ME_g$) for Dorper and Hu crossbred female lambs ranged between the NRC (2007) recommendation for early and later maturating growing sheep.

A Textile Analysis of Woolen Tapestry Curtain in Seoul Museum of Craft Art (서울공예박물관 소장 모담방장(毛毯房帳) 직물 분석)

  • An, Boyeon;Lee, Jangjon;Lee, Ryangmi
    • Journal of Conservation Science
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    • v.35 no.6
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    • pp.612-620
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    • 2019
  • A woolen tapestry curtain, owned by the Seoul Museum of Craft Art, is composed of tabby by cotton-warp and wool-weft threads and its patterned part is shown as a tapestry; paint with pigment has been added to it. The chromaticity of this curtain was measured and the substances in the deep red color were confirmed as Hg by an analysis of the ingredients through X-ray fluorescence. This is presumed to be cinnabar or vermilion. Analyses were performed on a total of seven fabric samples, including the warp & weft of the fabric, its trimming, and its back fabric. As a result, the warp of the woolen tapestry curtain was determined to be a cotton fiber with a middle hole or lumen in the cross-section. Furthermore, an infrared peak likewise showed O-H and C-O binding. Wool fibers as wefts were identified with circular and oval cross-sections and IR peaks showed N-H/O-H stretching and amide(-CONH-). The animal hair samples used in the wool fiber are believed to have come from long-tailed goral or goats and the possibility of using easy-to-spin sapsal dog hair is also not to be overlooked. This was determined through a contrast analysis by the Cultural Heritage Administration to identify the animal species used in the tapestry.

Conceptus-related measurements at early pregnancy in Black Bengal goat: an abattoir study

  • Talukder, Anup K.;Rahman, Mohammad A.;Hoque, Mohammad N.;Islam, Mohammad T.;Rahman, Abu N.M.A.;Das, Ziban C.
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.2
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    • pp.177-182
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    • 2020
  • The present study aimed to investigate the conceptus-related changes during early pregnancy in the Black Bengal breed of goat. A total of 22 gravid genitalia of the Black Bengal goats were collected from local slaughterhouses. The crown-rump lengths (CRL) of the conceptuses were determined to estimate the gestational age (GA). The length and diameter of uterine horn and amniotic sac were measured, and volume of amniotic and allantoic fluid formed by individual conceptus were recorded. The results reveal that the CRL is positively correlated with GA of the conceptus (R2 = 0.89, p < 0.05); however, CRL was not influenced by number of conceptus. Both the left and the right uterine horn gradually increased in size with the advancement of pregnancy irrespective of conceptus number present in the horn. The size of the amniotic sac of conceptus gradually increased with the conceptus age but maintained spherical shape from 5 to 7 weeks of pregnancy. The amniotic fluid formed by individual conceptus rapidly increased from 5 weeks (3.4 ± 0.3 mL) to 7 weeks (21.0 ± 2.0 mL) and 9 weeks (111.5 ± 4.0 mL). The volume of allantoic fluid formed by individual conceptus was steadily increased until 7 weeks (60.0 ± 5.0 mL) and began to decline slowly thereafter (50.0 ± 5.0 mL at 9 weeks). Notably, there was no effect of conceptus number per pregnancy on individual amniotic and allantoic fluid volume. The cotyledons have first appeared on the allanto-chorionic surface from 4 to 5 weeks of pregnancy. The closed eye, nostril and hooves of the conceptus became visible at 7 weeks of pregnancy. The present study has shown the basic information on conceptus-related developmental changes during early pregnancy up to 9 weeks in Black Bengal goat.

Effect of Genetic and Non-Genetic Factors Other Than Disease on Kid Survivability in Goat (산양의 생존율에 대한 유전적 및 비유전적 요인)

  • Miah, G.;Husain, S.S.;Hoque, M.A.;Baik, D.H.
    • Journal of Animal Science and Technology
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    • v.44 no.3
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    • pp.271-278
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    • 2002
  • This experiment was conducted to investigate the genetic and non-genetic factors affecting kid survivability in goats from birth to 90 days of age. The purebreds had lower survivability than the crossbreds, with significant (p$<$0.05) difference amongst themselves for the age of 31 to 90 days. The locational effect on survivability was insignificant for all the periods except 16 to 60 days (p$<$0.05) of age. The sex and birth type also had significant (p$<$0.05) effect on survivability for all the periods except 0 to 7 days of age. The survivability was found to be significantly (p$<$0.05) higher for kids born in summer season followed by those born in winter and rainy season. Milk produced by the does were significantly (p$<$0.05) affected on the survivability of kids during the period from 16 to 60 days of age. The survivability of kids were highest and lowest having milk yield of doe found to be 400-600 g/day and 80-200 g/day, respectively. Birth weight had significant (p$<$0.05) effect on survivability for all the stages of growth up to 90 days of age. Survivability was positively correlated with does’ milk yield as well as kids birth weight. Interactions of sex with location or birth type were significant (p$<$0.01 and p$<$0.05, respectively) though interaction between sex and genetic group was insignificant.

Course and Distribution of Facial Nerve of the Korean Native Goat (한국재래산양 두부의 안면신경 분포에 관한 해부학적 연구)

  • Lee, Heung-shik;Lee, In-se;Kim, Dae-joong
    • Korean Journal of Veterinary Research
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    • v.26 no.1
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    • pp.1-9
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    • 1986
  • This study was carried out to investigate the branch and distribution of Nervus facialis of the Korean native goat. The observation was made by dissection of embalmed cadavers of ten Korean native goats. The results were as follows; 1. N. facialis arose from the ventrolateral surface of the medulla oblongata. 2. In the facial canal, N. facialis gave off N. petrosus major, N. stapedius and Chorda tympani. 1) N. petrosus major arose from Ganglion geniculi, passed through the pterygoid canal and terminated in Ganglion pterygopalatinum. 2) Chorda tympani joined N. lingualis at the lateral surface of the internal pterygoid muscle. 3. At the exit of the stylomastoid foramen, N. facialis gave off N. caudalis auricularis, Ramus auricularis internus, Ramus stylohyoideus and Ramus digastricus. 1) N. caudalis auricularis arose by two branches in 6 cases and by a single branch in 4 cases. N. caudalis auricularis gave off branches to the caudoauricuIar muscles and the internal surface of the conchal cavity. 2) Ramus auricularis internus arose by a single branch except in 2 cases in which it arose in common with N. caudalis auricularis. It penetrated the caudolateral surface of the tragus and distributed in the skin of the scapha. 3) Ramus stylohyoideus and Ramus digastricus arose separately from N. facialis. 4. In the deep surface of the parotid gland, N. facialis divided into N. auriculopalpebralis, Ramus buccalis dorsalis and Ramus buccalis ventralis. In 6 cases, N. facialis gave off Ramus buccalis ventralis and then divided into N. auriculopalpebralis and Ramus buccalis dorsalis. In 3 cases, N. facialis trifurcated into Ramus buccalis ventralis, Ramus buccalis dorsalis and N. auriculopalpebralis. In one case, N. facialis gave off N. auriculopalpebralis and then divided into Ramus buccalis dorsalis and Ramus buccalis ventralis. 1) Ramus buccalis ventralis ran along the ventral border of the masseter muscle and distributed to the buccinator and depressor labii inferioris muscles. Ramus buccalis ventralis communicated with a branch of Ramus buccalis dorsalis and N. buccalis. In 2 cases, it also communicated with N. mylohyoideus. 2) Ramus buccalis dorsalis communicated with Ramus transverses faciei, N. buccalis, N. infraorbitalis and a branch of Ramus buccalis ventralis. Ramus buccalis dorsalis distributed to the orbicularis oris, caninus, depressor labii inferioris, levator labii superioris, buccinator, malaris, nasolabialis and zygomaticus muscles. 3) N. auriculopalpebralis gave off Rami auriculares rostrales, which supplied the zygomaticoauricularis muscle, the frontoscutularis muscle and the skin of the base of the ear. N. auriculopalpebralis then continued as Ramus zygomaticus, which innervated the frontal muscle, the lateral surface of the base of the horn, the orbicularis oculi muscle and the adjacent skin of the orbit. N. auriculopalpebralis communicated with Nn. auriculares rostrales and Ramus zygomaticotemporalis. In 7 cases, it also communicated with N. infratrochlearis.

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Effects of Activated Carbon and Charcoal on the Nutrients Utilization and Ruminal Fermentation Characteristics in Goat (활성탄 및 목탄의 첨가가 산양의 영양소 이용율 및 반추위내 발효성상에 미치는 영향)

  • Cha, Sang-Woo;Lee, Soo-Kee
    • Korean Journal of Agricultural Science
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    • v.32 no.2
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    • pp.197-203
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    • 2005
  • This study was conducted to investigate the effects of the addition of activated charcoal (AC) and oak charcoal on the ruminal fermentation characteristics, nutrient utilization in Korean native goats. Compared to reference diet, digestibility of dry matter, and crude protein in AC and charcoal diets tended to incraese. However, any tendency in ruminal degradation of crude fat was not observed. Ruminal degradation of NDF in AC diets tended to be more increased than that in non-AC diet. Although it was not significant, ADF tended to be increased in AC and charcoal diets. AC and charcoal did not affect the ruminal pH and ammonia-N. Concentration of total VFA and butyrate tended to be increased by adding AC and charcoal(p<0.05). Although it was not significant, acetate/propionate ratio tended to decreased in AC and charcoal diets. Although there appeared some beneficial effects in adding AC and charcoal to ruminant diets in this study, more works could be needed with AC before we can make clear conclusion on use of AC and charcoal in the ruminant diets.

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