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http://dx.doi.org/10.5713/ajas.2004.760

Accuracy of Ultrasonography in Early Pregnancy Diagnosis in Doe  

Singh, N.S. (Department of Livestock Production and Management College of Veterinary Science and Animal Husbandry)
Gawande, P.G. (Department of Veterinary Physiology, College of Veterinary sciences and Animal Husbandry)
Mishra, O.P. (Department of Veterinary Physiology, College of Veterinary sciences and Animal Husbandry)
Nema, R.K. (Department of Veterinary Physiology, College of Veterinary sciences and Animal Husbandry)
Mishra, U.K. (Department of Veterinary Physiology, College of Veterinary sciences and Animal Husbandry)
Singh, Mohan (Department of Veterinary Physiology, College of Veterinary sciences and Animal Husbandry)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.17, no.6, 2004 , pp. 760-768 More about this Journal
Abstract
The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.
Keywords
Crown Rump Length; Doe; Early Pregnancy; Trunk Diameter; Uterine Diameter and Ultrasonography;
Citations & Related Records

Times Cited By Web Of Science : 7  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
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1 Buckrell, B. C. 1988. Application of ultrasonography in reproduction in sheep and goats. Theriogenology 29:71-84.   DOI   ScienceOn
2 Fakruddin. 1996. The Livestock and Poultry Wealth. 1st Edn. NBS Publishers and Distributors, Bikaner. p. 137.
3 Garcia, A., M. K. Neary, G. R. Kelly and R. A. Pierson. 1993. Accuracy of ultrasonography in early pregnancy diagnosis in the ewe. Theriogenology 39:847-861.
4 Kahn, W., B. Kahn, A. Richter, J. Schulz and M. Wolf. 1995. Sonography during the pregnancy of sheep. I. Fetometry for the determination of the stage of gestation and prediction of the time of parturition. Tierklinik. Munchen 20:115-123.
5 Madel, A. J. 1983. Detection of pregnancy in ewe lambs by Amode ultrasound. Vet. Rec. 112:11-12.   DOI   PUBMED   ScienceOn
6 Perrera, B. M. A., T. A. Bongso and P. Abeynaike. 1978. Oestrus synchronization in goats using Cloprostenol. Vet. Rec. 102: 314.
7 Sergeev, L., D. O. Kleeman, S. K. Walker, D. H. Smith, T. L. Grosser, T. Mann and R. F. Seamark. 1990. Real time ultrasound imaging for predicting ovine foetal age. Theriogenology 34:593-601.
8 Singh, Yashwant, D. N. Sharma and L. D. Dhingra. 1979. Morphogenesis of testis in goat. Indian J. Anim. Sci. 49(11):925-931.
9 Trapp, M. J. and A. L. Slyter. 1983. Prgnancy diagnosis in the ewe. J. Anim. Sci. 57:1-15.
10 Wani, G. M., H. Gelderman. and J. Hann. 1985. Oestrus synchronization in goat Zuchthygiene 20(5):247-250.
11 White, I. R., A. J. F. Russel, I. A. Wright and J. K. Whyte. 1985. Real time ultrasonic scanning in the diagnosis of pregnancy and estimation of gestational age in cattle. Vet. Rec. 117:5-7.   DOI   ScienceOn
12 Aiumlamai, S., G. Fredriksson and L. Nilsfors. 1992. Real time ultrasonography for determining gestational age of ewes. Vet. Rec. 131:560-562.
13 Jainudeen, M. R., M. Wahid and E. S. E. Hafez. 2000. Sheep and Goats. In: Reproduction in farm animals. (Ed. B. Hafez and E. S. E. Hafez),7th edn. Lea and Febiger, Philadelphia.
14 Lindahl, I. L. 1971. Pregnancy diagnosis in ewe by intrarectal Doppler. J. Anim. Sci. 32:922-925.   DOI   PUBMED
15 Doize, F., D. Vaillancourt, H. Carabin and D. Belanger. 1997. Determination of gestational age in sheep and goats transrectal ultrasonographic measurement of plancentome. Theriogenology 48:449-460.
16 Ford, E. J. H. 1983. Pregnancy toxaemia. In: Martin, W.B.: Diseases of sheep. Blackwell Scientific Publications, London, pp. 147-151.
17 Kaulfuss, K. H., K. Uhlich, S. Brabant, K. Blume and K. Strittmatter. 1999. Real time ultrasonic pregnancy diagnosis (B-mode) in sheep.1. Frequent examination during the first month of pregnancy. Tiermedizinisches Zentrum. Wittenburg. 30:275-286.
18 Martinez, M. F., P. Bosch and R. A. Bosch. 1998. Determination of early pregnancy and embryonic growth in goat by transrectal ultrasound scanning. Theriogenology 49:1555-1565.
19 Na, J. S., J. S. Kim and B. K. Kang. 1987. Embryo Transfer in Korean Native goats. Kor. J. Anim. Sci. 29(7):295-302.
20 Logue, N. D., J. T. Hall, S. McRoberts and H. Waterhouse. 1987. Real time ultrasonic scanning in sheep: The results of first year of its application on farms in South West Scotland. Vet. Rec. 121:146-149.
21 Zipper, N., K. H. Kaulfuss, J. May and K. Elze. 1997. Real time ultrasonic pregnancy diagnosis in sheep. Part 3: diagnosis of the numbers of embryos and fetuses. Tierarztliche-Praxis. 25(3):213-222.
22 White, I. R., A. J. F. Russel and D. G. Fowler. 1984. Real time ultrasonic scanning in the diagnosis of pregnancy and determination of foetal numbers in sheep. Vet. Rec. 115:140-143.
23 Gade, S. N. and R. C. Takarkheda. 2002. Efficacy of $PGF_{2}{\alpha}$ on estrus synchronization in Osmanabadi goats. Indian Vet. J. 79 (9):967.
24 Roy, R. 1991. Indian dairyman. 13:48-49. Cited by B. Prakash and D. S. Balain. 1992. In conservation, evaluation and utilization of goat germ plasm resource in India. Indian J. Anim. Prod. Mgmt. 8:1-22.
25 Watt, B. R., G. A. Anderson and I. P. Campell. 1984. A comparison of six methods used for detecting pregnancy in sheep. Aust. Vet. J. 61:377-381.
26 Haibel, G. K. 1990. Use of ultrasonography in the reproductive management of sheep and goats herd. Veterinary Clinics of North America. Food Anim. Pract. 6:97-613.
27 Ginther, O. J. and S. A. Pierson. 1984. Ultrasonic anatomy of equine ovaries. Theriogenology 21:471-483.
28 Johns Michael. 2002. Pregnancy diagnosis in ewes. Farm note 74/99. Deptt of Agril. West Australia.
29 Davey, C. G. 1986. An evaluation of pregnancy testing in sheep using a real time ultrasound scanner. Aust. Vet. J. 63:347-348.   DOI   PUBMED   ScienceOn
30 Shin, S. T., H. G. Shin, Y. S. Choi, H. S. Yang, S. K. Jang, O. K. Lee, J. H. Lim, S. W. Jin, C. S. Lee and Y. W. Lee. 2001. Ultrasound for detecting the early pregnancy and estimating the fetal age in Korean Black goats. Theriogenology 55: 533.
31 Schrick, F. N. and E. K. Inskeep. 1993. Determination of early pregnancy in ewes utilizing transrectal ultrasonogrsphy. Theriogenology 40:295-306.
32 Ishwar, A. K. and J. N. Pandey. 1990. Estrus synchronization and fertility behavior in Black Bengal following either progesterone or prostaglandin treatment. Theriogenology 34 (5):1015-1023.
33 Mia, M. M., A. Ali and A. K. F. H. Bhuiyan. 1996. The reproductive performance of Black Bengal, Barbari, Barbari X Black Bengal and Anglo Nubian goats. Indian Vet. J. 73: 1048-1052.
34 Rainio,Vesa. 1992. Estrus synchronization in Finn sheep with cloprostenol. Theriogenelogy 37:280.
35 Kareni, A., P. Kovacs, J. F. Becker and O. Szencii. 2001. Pregnancy diagnosis in sheep: Review of the most practicable methods. Acta Vet. Brno. 70:115-126.
36 Refstal, K. R., J. V. Martiuk, C. S. F. Williams and R. F. Nachreiner. 1991. Concentration of estrone sulphate in peripheral serum of pregnant goats: relationship of gestational length, foetal number and the occurrence of foetal death in utero. Theriogenology 36:449-461.
37 Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods. 8th Edn. IOWA. State University Press, Ames, IOWA.
38 Grace, N. D., A. D. Beach, T. D. Quinlivan and B. Ward. 1989. Multiple pregnancy diagnosis of ewes using real time ultrasonic body scanner and video- fluoroscopy systems. Proceeding of New-Zealand Society of Animal Production 49:107-111.
39 Biswas, J. C. and G. L. Koul. 1994. Note on gestation length of pashmina producing Himalayan Chegu goats. I. J. Anim. Hlth. 33(2 ):129-130.
40 Humbolt, P., G. de Montigny, F. Tetedoic, B. Payen, M. Thibier and R. G. Sasser. 1990. Pregnancy specific protein B and progesterone concentrations in French Alpine goats throughout gestation. J. Reprod. Fert. 89:205-212.
41 Gonzalez, B. A., M. J. Santiago and S. A. Lopez. 1998. Estimation of fetal development in Manchega dairy ewes by transrectal ultrasonographic measurements. Small Rumin. Res. 27:243-250.
42 Richardson, C. 1972. Diagnosis of pregnancy in the ewe by vaginal biopsy. Br. Vet. J. 128:316-329.
43 Jitendra Kumar, R. K. Chandolia, S. K. Verma, I. S. Lohan, J. S. Rana and D. C. Bhatia. 2000. Ultrasonogrsphic studies on early fetal development in goat. Paper presented in XVI Annual convention and National symposium of ISSAR on Reproduction management for optimizing production from livestock. Nov. 6-8. BAU. Ranchi. India.
44 Smith, M. C. 1980. Caprine reproduction.In: (Ed. D. A. Morrow), Current therapy in Theriogenology (1st edn.) W.B. Saunders, Philadelphia, P.A. pp. 975-977.
45 Evans, H. E. and W. O. Sack. 1973. Prenatal development of domestic and laboratory mammals. Anat. Histol. Embryol. 2:11-15.
46 Goel, A. K. and K. P. Agrawal. 1992. A review of pregnancy diagnosis techniques in sheep and goats. Small Rumin. Res. 9:255-264.
47 Deas, D. W. 1977. Pregnancy diagnosis in the ewe by an ultrasonic rectal probe. Vet. Rec. 101:113-115.   DOI   PUBMED   ScienceOn
48 Meredith, M. J. and M. O. K. Madani. 1980. The detection of pregnancy in sheep by A-mode ultrasound. Br. Vet. J. 136:325-330.
49 Murray, R. D. and R. Newstead. 1988. Determination of steroid hormones in goat milk and plasma as an aid to pregnancy diagnosis using an ELISA. Vet. Rec. 122:158-161.
50 Phalak, K. R., A. D. Patil, B. P. Kurhe and R. L. Dhoble. 2000. Efficacy of $PGF_{2}{\alpha}$ through IVSM route for oestrus synchronization in Osmanabadi goats. Intas Polivet 1:313-314.