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Comparison of Diluents on Liquid Storage of Korean Native Goat Spermatozoa  

Kim, H.J. (National Livestock Research Institute)
Choe, J.Y. (National Livestock Research Institute)
Choi, S.H. (National Livestock Research Institute)
Son, D.S. (National Livestock Research Institute)
Choi, S.H. (National Livestock Research Institute)
Sang, B.D. (National Livestock Research Institute)
Han, M.H. (National Livestock Research Institute)
Ryu, I.S. (National Livestock Research Institute)
Kim, I.C. (National Livestock Research Institute)
Kim, I.H. (College of Veterinary Medicine, Chungbuk National University)
Im, K.S. (Seoul National University)
Kim, S.J. (National Livestock Research Institute)
Cho, S.R. (National Livestock Research Institute)
Publication Information
Journal of Embryo Transfer / v.21, no.4, 2006 , pp. 339-344 More about this Journal
Abstract
This study was to investigate the optimal short-term storage diluents for goat spermatozoa. Semen was collected with electro-ejaculation from two goats. The collected semen was diluted in BTS and centrifuged at 500 g for 5 minutes. The supernatant was discarded and diluted with BTS, Modena or Triladyl$^{(R)}$ and stored at 4 or $17^{\circ}C$ for 8 days. The motility of spermatozoa in BTS and Modena stored at $4^{\circ}C$ was rapidly decreased at day 1. The motility of spermatozoa in BTS at $17^{\circ}C$ was decreased to 30$\sim$45% at day 2. In Modena at $17^{\circ}C$, the inappropriate motility fur artificial insemination (AI) was reached at day 4. The spermatozoa stored in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ were more viable and higher motility up to day 4. In conclusion, liquid storage of goat spermatozoa in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ for 4 days showed permissible viability for AI.
Keywords
spermatozoa; liquid storage; motility; diluents; Korean native goat;
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