• 한국식품영양과학회지
• /
• 제35권2호
• /
• pp.145-149
• /
• 2006

발효시킨 포도를 첨가하여 흑염소 육의 품질특성을 조사하였다. 육질을 조사한 결과 대조구와 비교하여 발효시킨 포도사료의 식이량이 증가할수록 점차적으로 경도가 감소하는 경향으로 나타나 발효시킨 포도사료의 식이량이 육의 조직에 매우 많은 영향을 미치는 것을 알 수 있었다. 흑염소 육의 pH는 $6.1\~6.4$ 사이로 정상육 범위내에 속하는 것으로 조사되었으며, 대조구와 발효시킨 포도사료 처리구간의 유의적인 차이는 나타나지 않았다. 흑염소 육의 주요 무기성분은 Ca, Na, K 및 Mg로 나타났으며, 특히 T2 처리구에서 매우 높은 칼슘 증가 현상을 볼 수 있었다. 발효시킨 포도사료를 급여한 흑염소 육의 총 아미노산 함량은 대조구의 $6,880.37\;mg\%$보다 T1과 T2 처리구에서 각각 7,994.20와 $8,288.16\;mg\% $로 총 아미노산 함량이 증가하는 경향을 나타내었다. 발효시킨 포도사료를 식이한 흑염소 육의 지방산은 대조구와 비교하여 불포화지방산의 함량이 증가하는 것으로 나타났다. 색도는 대조구에 비하여 발효시킨 포도사료의 식이량이 증가할수록 명도값이 증가하는 경향을 나타내어 발효시킨 포도사료의 첨가에 의하여 흑염소 육의 색깔이 선명해지는 것을 알 수 있었으며, 일반사료를 급여한 대조구와 포도 발효사료를 식이한 흑염소 등심을 이용하여 관능검사 결과 포도발효과피 $30\%$ 첨가구인 T3구에서 높은 관능검사 결과를 나타내었다.

• 생명과학회지
• /
• 제30권11호
• /
• pp.1007-1011
• /
• 2020

본 연구는 우리나라 고유의 재래흑염소 집단인 당진, 장수, 통영 및 경상대 계통과 교잡종 염소 계통 또는 해외품종의 개체 식별을 위한 유전적 다양성과 관계 조사 및 검증을 위해 수행하였다. 각 염소 집단에 존재하는 Monomorphic SNP를 수집한 이후 공통적으로 존재하는 SNP 133개를 선발하여 분석에 이용하였다. Monomorphic SNP 133개를 통한 재래흑염소와 교잡종 염소의 유전적 구조 차이를 나타냈으며, 주성분 분석 결과 재래흑염소와 교잡종 염소가 명확히 구분되는 것으로 나타났다. 또한, 참조집단 이외의 70두(Native Korean goat = 24, Cross breed = 46)로 구성된 검증집단을 분석한 결과 국내 재래흑염소 계통의 참조집단과 동일한 유전적 구조를 나타냈으며, 교잡종 염소의 경우 참조집단의 일부 유전적 구성을 공유하는 것으로 나타났다. 국내 재래흑염소의 경우는 하나의 군집을 형성한 반면 해외 품종 및 교잡종 계통의 경우 재래흑염소 계통에 비해 넓게 퍼져 군집을 형성하는 것으로 나타났다. 따라서, 본 연구 결과는 국내 재래흑염소 유전자원 집단을 보존을 위한 기초자료로 활용하고 추후 유전적 다양성을 고려한 염소의 개량을 위한 기초자료로 유용하게 활용 할 수 있을 것으로 판단된다.

• 한국식품위생안전성학회지
• /
• 제37권6호
• /
• pp.411-417
• /
• 2022

본 연구는 생후 12개월령의 염소를 사용하여 앞다리, 뒷다리, 등심 및 갈비 부위로 분할하여 in vitro 소화실험을 통해 부위별 단백질 가수분해도 및 아미노산 조성을 조사하였다. 이 때, 소고기 및 돼지고기의 분할육을 이용하여 염소고기와 비교, 분석하였다. 염소고기 분할육 중 뒷다리(8.32%) 및 갈비(8.32%)가 가장 높게 단백질 가수분해도가 나타났으며, 염소고기의 갈비 부위는 갈비 분할육 중 가장 높은 단백질 가수분해율을 보였던 돼지고기(8.57%)와 유의 차가 없었다 (P>0.05). In vitro 소화 전에는 염소고기 분할 육 중 등심에서 글리신(11.03%)이, 앞다리에서 글루타민(53.44%)이 다른 고기 종류 및 분할육들에 비해 유의적으로 높은 비율로 포함된 것이 확인되었다(P<0.05). In vitro 소화 후에는 염소고기 갈비 부위에서 라이신(17.54%)이 가장 높은 비율로 포함된 것으로 확인되었으며, 소 갈비 부위보다 유의적으로 높았다(P<0.05). 본 연구는 염소고기 분할육의 단백질 가수분해도 및 아미노산 조성을 제공하며 단백질 소화양상 및 생체 이용률을 평가하기 위한 기초 자료로써 활용되어질 수 있을 것으로 사료된다.

• 대한수의학회지
• /
• 제51권4호
• /
• pp.277-280
• /
• 2011

Brucellosis is the most important bacterial disease of livestock in Bangladesh. The present study was conducted to determine the seroprevalence of brucellosis in goat and sheep in Mymensingh and Bogra districts of Bangladesh using slow agglutination test and Rose Bengal test as screening test and indirect enzyme linked immunosorbent assay as confirmatory test. Questionnaire based data on age, gender, area, client's complaint, number of animals in herds, disease history, reproductive problems such as abnormal uterine discharge, abortion or previous abortion in sheep and goat and their reproductive diseases were recorded. A total of 200 sera samples were collected from 80 sheep and 120 goats. The prevalence of brucellosis in goat was 2.50% and 1.25% in sheep. Positive reactors were only detected in female of both goat and sheep. In this study, there existed a significant association among abortion and the prevalence of brucellosis (p < 0.01). The prevalence of brucellosis in sheep and goat in Bangladesh is not negligible, and it is therefore worth considering the adoption of preventive measures.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권7호
• /
• pp.905-911
• /
• 2011

Polymorphisms of BMP15 gene exon 2 and its relationship with prolificacy of goats were detected by PCR-SSCP and DNA sequencing methods in Chinese two local goat breeds. The results showed that the product amplified by the primers displayed polymorphisms. Three genotypes (AA, BB and AB) were detected in Funiu white goats, and their frequency was 0.071, 0.715, 0.214, respectively. Two genotypes (AB and BB) were detected in Taihang black goats, and their frequency was 0.342 and 0.658, respectively. Sequencing revealed that four mutations (456T${\rightarrow}$G, 466C${\rightarrow}$G, 510C${\rightarrow}$T, 511T${\rightarrow}$C) occurred in genotype BB of Funiu white goat, which resulted in amino acid substitution of V155G and S171P. No mutation was detected in Taihang black goat. The Funiu white goat with genotype BB had 0.91 or 0.82 kids, more than those with AB or AA, respectively. The difference of the least squares means for litter size between BB and AB was not significant (p>0.05) in Taihang black goat. It is concluded that the BMP15 gene may be a major gene which affects the prolificacy in Funiu white goats. This study could provide basic molecular data on the reproductive characteristics of local breeds of Henan province in China, and a scientific basis for the conservation and utilization of those two goat breeds.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권6호
• /
• pp.842-849
• /
• 2007

The purpose of the present study was to produce live offspring from in vitro fertilized goat embryos. Oocytes were collected from abattoir ovaries and kept in oocyte collection medium. Oocytes were washed 4-5 times with maturation medium containing medium-199 with 5 ${\mu}g/ml$ FSH, 100 ${\mu}g/ml$ LH, 1 ${\mu}g/ml$ estradiol-$17{\beta}$ 50 ${\mu}g/ml$ gentamycin, 10% inactivated estrus goat serum, and 3% BSA (fatty acid free). Oocytes were placed in 100 ${\mu}l$ drops of maturation medium containing granulosa cell monolayer and incubated in a 5% $CO_2$ incubator at $38.5^{\circ}C$ for 27 h. For capacitation of spermatozoa fresh semen was processed and mixed in 3 ml fertilization TALP medium containing 50 ${\mu}g/ml$ heparin and kept in the above incubator for 2 h. The capacitated spermatozoa were coincubated with matured oocytes for fertilization. Cleaved embryos were separated and cultured in embryo development medium with oviductal cells and 494 embryos were produced. Recipient goats were synchronized with two injections of 15 mg $PGF_{{2}{\alpha}}$/goat 10 days apart. Eighty early stage embryos were transferred into the uterotubal junction of 14 surrogate mothers using laparoscopy techniques. One recipient delivered twin kids, whereas another two recipients each.delivered a single kid The parentage of these kids was evaluated using highly polymorphic co-dominant microsatellites markers. From the present study, it was concluded that live goat kids can be produced from in vitro matured and fertilized goat embryos, to the best of our knowledge for the first time in India.

• 한국축산식품학회지
• /
• 제37권6호
• /
• pp.948-954
• /
• 2017

The objective of this study was to investigate the relationship between muscle fiber characteristics and fatty acid composition of four major muscles in Korean native black goat (KNBG). Longissimus lumborum (LL), psoas major (PM), semimembranosus (SM), and gluteus medius (GM) were obtained from five male KNBGs of 36 mon of age and subjected to histochemical analysis and to determine fatty acid composition and meat quality traits. There were significant (p<0.05) differences in fiber number percentage (FNP) and fiber area percentage (FAP) of fiber types among these four muscles. PM had the highest FNP of type I and the lowest FNP of type IIB, while SM had the highest FNP of type IIB. The highest fat content was observed in LL while SM had the lowest fat content. The proportions of SFA and MUFA were significantly (p<0.05) different among four muscles due to differences in the majority of fatty acids such as oleic (C18:1) and palmitic (C16:0) acids. The PUFA/SFA ratio was significantly (p<0.05) different among four muscles, and the highest PUFA/SFA ratio was observed in PM. Results suggested that LL and PM might be healthful because of higher desirable fatty acid value and PUFA/SFA ratio, respectively. Also, data showed that correlations between muscle fiber types and fatty acids proportion of goat muscles were reversed with those of cattle muscles.

• 한국식품과학회지
• /
• 제32권3호
• /
• pp.538-543
• /
• 2000

가열 처리된 우육, 돈육, 계육, 양육과 염소육과의 종판별을 위한 효소면역측정법(ELISA)을 확립하기 위하여 염소육을 $98^{\circ}C$에서 15분간 열처리해서 추출액을 분리하였다. 열에 안정한 주요 단백질(TS)은 분자량이 36과 38kDa이었고 두 분자량대의 단백질의 pI값은 4.5로 확인되었으며 면역원으로 사용하기 위해 이온교환 (DEAE-Sephadex A-50)과 겔 여과(Sephadex G-75) 크로마토그래피로 정제하였다. 분리, 정제된 TS단백질을 토끼에 면역하여 염소육에 대해 특이성을 갖는 항체를 생산하고 항TS 항체를 이용하여 간접경합 ELISA를 확립하였다. 항TS 항체는 가열 처리된 계육, 우육, 돈육, 양육과는 반응성이 전혀 없었고 동일하게 처리된 염소육과 지표단백질인 TS에만 반응성을 보였다. 따라서, 본 연구에서 확립한 간접경합 효소면역측정법은 가열 처리된 육류 중 염소육 판별에 웅용 가능함을 시사하였다.

• 한국임상수의학회지
• /
• 제20권3호
• /
• pp.317-322
• /
• 2003

Cytogenetic and hematological analysis was performed in peripheral blood from the Korean native goat bred in the nuclear power plant (Wolsong and Uljin) and control area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from goat was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 to 4 Gy from the goat lymphocytes with in vitro irradiation. MN rates in goats from the Wolsong and Uljin nuclear power plant, and control area were 9.60/1000, 6.83/1000 and 9.88/1000, respectively. There were no significant differences in MN frequencies and hematological values in goats between nuclear power plant and control area. High level of platelet in the goat from Uljin nuclear power plant was observed, which seemed to be related to the goat management.

• Journal of Animal Science and Technology
• /
• 제47권1호
• /
• pp.83-90
• /
• 2005

ACE-inhibitory peptides derived from goat's whey hydrolyzed by various proteolytic enzymes were separated and purified for antihypertension materials. The highest ACE-inhibitory activity of goat's whey hydrolysates was 85.5 % by pepsin for 72 hrs. Also the highest ACE-inhibitory activity of goat's whey hydrolysates was F-4 by pepsin for 72 hrs by Sephadex G-25 gel chromatograms. F-4e and F-4ed from F-4 by RP-HPLC to first and second purification were the highest in ACE-inhibitory activity, respectively. The most abundant amino acid was leucine(I 8.54 %) in F-4ed of ACE-inhibitory peptides after second purification. Amino acid sequence of F-4ed of ACE-inhibitory peptides showed Leu-Lys-Asp-Tyr-Gly-GlyVal- Ser-Leu and Leu-Gly-Asp-Gly-Ala-Gly- Asp-Val-Ala-Phe. $IC_{50}$ calibrated in peptic hydrolysates(72 hrs), F-4, F-4e and F-4ed from goat's whey hydrolysates by pepsin for 72 hrs were 33.93, 28.75, 11.74 and 1.09 mg/ml, respectively. From the results of this experiment, goat's whey hydrolysate by pepsin was shown to have ACE-inhibitory activity.