• Title/Summary/Keyword: glyceride

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Glycerides from the Aerial Parts of Garland (Chrysanthemum coronarium L.) and Their Inhibitory Effects on ACAT, DGAT, FPTase, and $\beta$-Secretase

  • Song, Myoung-Chong;Yang, Hye-Joung;Cho, Jin-Gyeong;Chung, In-Sik;Kwon, Byoung-Mog;Kim, Dae-Keun;Baek, Nam-In
    • Food Science and Biotechnology
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    • v.18 no.1
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    • pp.95-102
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    • 2009
  • The aerial parts of garland (Chrysanthemum coronarium L.) were extracted in 80% aqueous methanol (MeOH) and the concentrated extract was then partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. EtOAc and n-BuOH fractions resulted in 4 glycerides with the application of octadecyl silica gel and silica gel column chromatography. The chemical structures of the glycerides were determined using several spectroscopic methods, including nuclear magnetic resonance (NMR) and mass spectrometry (MS) as (2S)-1-O-palmitoyl-sn-glycerol (1), (2S)-1-O-oleoyl-2-O-oleoyl- 3-O-$\beta$-D-galactopyranosyl-sn-glycerol (2), (2S)-1-O-palmitoyl-2-O-linoleoyl-3-O-phosphorouscholine-sn-glycerol (3), and (2S)-1-O-linolenoyl-2-O-palmitoyl-3-O-[$\alpha$-D-galactopyrasyl-($1{\rightarrow}6$)-$\beta$-D-galactopyranosyl]-sn-glycerol (4). The free fatty acids of these glycerides were determined with gas chromatography (GC)-MS analysis following alkaline hydrolysis and methylation. These glycerides demonstrated an inhibitory effect on acyl-CoA: cholesterol acyltransferase (ACAT, compound 1: $45.6{\pm}0.2%$ at $100{\mu}g/mL$), diacylglycerol acyltransferase (DGAT, compound 1: $59.1{\pm}0.1%$ at $25{\mu}g/mL$), farnesyl protein transferase (FPTase, compound 2: $98.0{\pm}0.1%$; compound 3: $55.2{\pm}0.1%$ at $100{\mu}g/mL$), and $\beta$-secretase ($IC_{50}$, compound 4: $2.6{\mu}g/mL$) activity. This paper is the first report on the isolation of these glycerides from garland and their inhibitory activity on ACAT, DGAT, FPTase, and $\beta$-secretase.

Effects of Various Emulsifiers on the Quality of Waxy Rice Cake (종류별 유화제가 찹쌀떡의 품질에 미치는 영향)

  • 신언환;황성연;최원균
    • The Korean Journal of Food And Nutrition
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    • v.14 no.1
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    • pp.40-45
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    • 2001
  • This study was conducted to investigate the effects of various emulsifiers on the quality of the waxy rice cake. Falling numbers of the waxy rice flour with monoglyceride, lecithin and control were not significantly different, but with sugar esther 0.5% and 1% showed higher value as 88.4 and 81 than control Initial pasting temperature of the waxy rice flour was 66.78$\^{C}$ and others were 66.45 ∼ 67.05$\^{C}$ by adding 0.5%, 1% of emulsifiers such as monoglyceride, lecithin, sugar esther. Waxy rice flour with 1% sugar ester showed the highest peak viscosity as like as falling number. Waxy rice cake wish various emulsifiers showed tendency to be slowly firming rate as compared with control. In all case, waxy rice flour with sugar ester 1% was considered to be more effective to the decrease of firming rate. Waxy rice flour with lecithin showed worse visual color than others and sugar ester provided best visual and sensory quality. After 5 days cold storage, waxy rice flour with sugar ester 1%\`s Aw was 0.875 and control\`s 0.911. These results suggested that water holding capacity of sugar ester was the best during storage.

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Preparation and Evaluation of $PGE_1$ Transurethral Suppositories (프로스타글란딘 $E_1$ 요도좌제의 제조 및 평가)

  • Kim, Jong-Oh;Quan, Qi-Zhe;Rhee, Jong-Dal;Choi, Han-Gon;Yong, Chul-Soon
    • Journal of Pharmaceutical Investigation
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    • v.30 no.3
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    • pp.173-178
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    • 2000
  • The purpose of this work is to develop a transurethral suppository containing prostaglandin $E_1\;(PGE_1)$, which stabilizes the drug, gives no irritation to physiological body and enhances the erectile response of $PGE_1.\;PGE_1$ transurethral suppositories were prepared with various amounts of compositions such as saturated polyglycolysed glyceride $(Suppocire^{\circledR}\;AP,\;SAP)$, polyoxyethylene hydrogenated castor oil (HCO-50) and ethanol. The melting points, viscosities and $PGE_1$ release of the suppositories were investigated. Ocular irritation test was carried out after application of $PGE_1$ suppository to rabbit's eye. The intracavernous pressure (ICP), penile length and duration of erectile response were determined after transurethral administration of $PGE_1$ suppository and compared with those after intracavernosal injection of $PGE_1$ solution to cats. HCO-50 hardly affected the melting points and viscosities of $PGE_1$ suppositories. Additionally, $PGE_1$ transurethral suppositories, whose melting point ranges was $34-35^{\circ}C$, was speedily melted in physiological body. HCO-50 significantly decreased the dissolution rates of $PGE_1$ from the suppositories. Dissolution mechanism analysis showed the release of $PGE_1$ was proportional to the square root of time, indicating that $PGE_1$ might be released from the suppositories by Fickian diffusion. The release rate of $PGE_1$ from $PGE_1$ suppository [PGE1/SAP/HCO-50/ethanol (1/94.5/2.5/2%)] was about 80% within 2 h. This $PGE_1$ suppository gave no significant irritation to the ocular tissue, expecting that it gave no irritation to the urethral tissue less sensive than ocular tissue. Furthermore, $PGE_1$ in this suppository was stable at $4^{\circ}C$ for 2 years. This suppository increased the ICP and penile erection similar to those of injectable $PGE_1$ solution. However, it gave 2.5-fold increased duration of erectile response than injectable $PGE_1$ solution. Our results suggested that it gave more effective erectile response than injectable $PGE_1$ solution in cats. It is concluded that this $PGE_1$ suppository with good safety, excellent stability and enhanced erectile response, could be a more effective and convenient transurethal delivery system of $PGE_1$.

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Analysis of the Lipid Components in Chestnut(Castanea crenata) -II. Lipid and Fatty Acid Composition of Neutral Lipid , Glycolipid and Phospholipid- (밤 지질(脂質) 성분(成分)의 분석(分析) -제2(二)보. 구성 지질(脂質) 및 구성 지방산(脂肪酸)의 조성(組成)-)

  • Rhee, Chong-Ouk;Kim, Eun-Seun;Kim, Dong-Youn
    • Applied Biological Chemistry
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    • v.26 no.1
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    • pp.19-27
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    • 1983
  • The lipid and fatty acid compositions of neutral lipid, glycolipid and phospholipid were identified and quantified by thin-layer chromatography and gas chromatography. Main constituents of the neutral lipid were triglyceride, free sterol and esterified sterol in the free lipid, and free fatty acid and monoglyceride in the bound lipid. In the outer part, there existed 25.7% triglyceride in the free lipid and it was not nearly found in the bound lipid. Main constitutents of the glycolipid were digalactosyl diglyceride and esterified steryl glycoside in the free lipid, and digalactosyl diglyceride in the bound lipid. Free lipid didn't contain trigalactosyl diglyceride but bound lipid contained 2.0% of it. Main constituents of the phospholipid were lysophosphatidyl choline, phosphatidyl inositol, phosphatidyl choline, phosphatidyl glycerol and phosphatidyl ethanolamine in the free lipid, and phosphatidyl choline and phosophatidyl inositol in the bound lipid. The predominant fatty acids of three fractions, neutral lipid, glycolipid and phospholipid showed almost the same pattern as that of the total free and bound lipids. The content of palmitic acid was relatively higher in the polar lipids(glyco and phospholipid). Therefore, saturated fatty acid ratio of polar lipid was higher than that of neutral lipid. found lipid contained more saturated fatty acids as compared with the free lipid.

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Glycosyl glycerides from hydroponic Panax ginseng inhibited NO production in lipopolysaccharide-stimulated RAW264.7 cells

  • Cha, Byeong-Ju;Park, Ji-Hae;Shrestha, Sabina;Baek, Nam-In;Lee, Sang Min;Lee, Tae Hoon;Kim, Jiyoung;Kim, Geum-Soog;Kim, Seung-Yu;Lee, Dae-Young
    • Journal of Ginseng Research
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    • v.39 no.2
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    • pp.162-168
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    • 2015
  • Background: Although the aerial parts of hydroponic Panax ginseng are reported to contain higher contents of total ginsenosides than those of roots, the isolation and identification of active metabolites from the aerial parts of hydroponic P. ginseng have not been carried out so far. Methods: The aerial parts of hydroponic P. ginseng were applied on repeated silica gel and octadecylsilane columns to yield four glycosyl glycerides (Compounds 1-4), which were identified based on nuclear magnetic resonance, infrared, fast atom bombardment mass spectrometry, and gas chromatography/mass spectrometry data. Compounds 1-4 were evaluated for inhibition activity on NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Results and conclusion: The glycosyl glycerides were identified to be (2S)-1-O-7(Z),10(Z),13(Z)-hexadecatrienoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (1), (2S)-1-O-linolenoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (2), (2S)-1-O-linolenoyl-2-O-linolenoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (3), and 2(S)-1-O-linoleoyl-2-O-linoleoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (4). Compounds 1 and 2 showed moderate inhibition activity on NO production in LPS-stimulated RAW264.7 cells [half maximal inhibitory concentration ($IC_{50}$): $63.8{\pm}6.4{\mu}M$ and $59.4{\pm}6.8{\mu}M$, respectively] without cytotoxicity at concentrations < $100{\mu}M$, whereas Compounds 3 and 4 showed good inhibition effect ($IC_{50}$: $7.7{\pm}0.6{\mu}M$ and $8.0{\pm}0.9{\mu}M$, respectively) without cytotoxicity at concentrations < $20{\mu}M$. All isolated compounds showed reduced messenger RNA (mRNA) expression of interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, and tumor necrosis factor-${\alpha}$ in LPS-induced macrophage cells with strong inhibition of mRNA activity observed for Compounds 3 and 4.

A Study on the Physicochemical Characteristics of Saw Palmetto Extract (쏘팔메토(Saw Palmetto) 열매 추출물의 이화학적인 특성 연구)

  • Jeong-Eun Lee;Jung-Uk Kim;Hee-Young Lee;Ji-Hye Eom;Jong-Gil Kim;Young-Yul Lee;Hyeon-Ji Bae;Seung-Woo Kim;Ho-Jeong Yun;Su-Mi Han;Jong-Ho Koh;Moochang Kook;Young-Sang Lee
    • The Korean Journal of Food And Nutrition
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    • v.36 no.3
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    • pp.202-208
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    • 2023
  • FT-IR, GC/MS, and ATR-FT-IR analyses were performed to confirm the physicochemical characteristics of saw palmetto fruit (SPF) extract. FT-IR analysis of the standard product showed that the band corresponding to the carbonyl bond of free fatty acid was stronger than the band of acyl-glyceride. Sample E was identified as having the same trend as the standard sample. Fatty acid composition analysis revealed that the main fatty acids in the standard sample were lauric acid and oleic acid. The content of lauric acid ranged from approximately 30% to 38% in samples B, C, D, and E, while the content of oleic acid ranged from approximately 29% to 34%. The GC/MS analysis confirmed that the standard SPF extract consisted of fatty acids and fatty acid ethyl esters. Sample E demonstrated a similar pattern to the standard samples in terms of oleic acid, lauric acid, and fatty acid esters. ATR-FT-IR analysis indicated that only sample E was predicted to contain 100% saw palmetto extract. Therefore, these study findings can be considered fundamental data for analyzing the physicochemical characteristics of the composition of SPF extract.

The effect of Galwhahaejungtang and exercise on variation of lipid, BUN and Creatinine in rat with alcohol administration (갈화해성탕(葛花解醒湯) 투여 및 운동 부하가 알코올 섭취 후 흰쥐의 혈청 지질, BUN, Creatinine 함량에 미치는 영향)

  • Han, Eul-Joo;Lee, Myeong-Jong
    • The Journal of Dong Guk Oriental Medicine
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    • v.9
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    • pp.111-125
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    • 2000
  • Galwhahaejungtang has been used to alcoholic disease. Object of This study is examining the effect of Galwhahaejungtang and exercise on variation of total cholesterol, HDL-cholesterol, triglyceride, free fatty acid, BUN, Creatinine in rat with alcohol administration. METHODS AND MATERIALS Fifty four rats were divided into four groups. 1. The group of rat with alcohol administration and no treatment 2. The group of rat with alcohol and Galwhahaejungtang administration 3. The group of rat with alcohol administation and exercise 4. The group of rat with alcohol, Galwhahaejungtang administration and exercise Serum of rat were obtained after 2 weeks and 4 weeks. RESULTS 1. The level of total cholesterol was decreased in the group of rat with Galwhahaejungtang administration and exercise after 2 weeks. After 4 weeks, the level of total cholesterol was significantly decreased in the group of rat with Galwhahaejungtang administration and exercise. 2. The level of HDL-cholesterol was significantly increased in the group of rat with exercise after 2 weeks. After 4 weeks, the level of HDL-cholesterol was significantly increased in the group of rat with no treatment and with Galwhahaejungtang administration. 3. The level of try glyceride was significantly decreased in the group of rat with exercise after 2 weeks. After 4 weeks, the level of tryglyceride was significantly decreased in the group of rat with Galwhahaejungtang administration. 4. The level of free fatty acid was significantly decreased in the group of rat with Galwhahaejungtang administration after 2 weeks. After 4 weeks, the level of free fatty acid was significantly decreased in the group of rat with Galwhahaejungtang administration and with Galwhahaejungtang administration and exercise. 5. The level of BUN was significantly decreased in the group of rat with Galwhahaejungtang administration and exercise after 2 weeks. After 4 weeks, the level of BUN was significantly decreased in the group of rat with exercise. 6. The level of creatinine was significantly decreased in the group of rat with Galwhahaejungtang administration and exercise. After 4 weeks, the level of creatinine was significantly decreased in the group of rat with exercise and with Galwhahaejungtang administration and exercise. CONCLUSION Based on these results, it is considered that Galwhahaejungtang administration and exercise has an accelerating effect on alcohol metabolism.

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Effects of Sowing Date on Grain Filling and Related Traits in Winter Barley (파종기 차이가 보리의 등숙과 등숙관련 형질에 미치는 영향)

  • 류용환;이창덕;하용웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.93-103
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    • 1992
  • Experiments were conducted in the research field of the Wheat and Barley Research Institute with three barley cultivars, Olbori, Kangbori and Suwon 18, which showed different growth characteristics. The highest rates of daily dry matter accumulation in grains were 1.03 1.94 mg / grain / day in early and optimum sowings and 0.88 1.88 mg / grain / day in late sowings, which occurred around 20~30 days after heading in early and optimum sowings and 15~20 days after heading in late sowings. Grains reached their maximum weight by 40 days after heading in early and optimum sowings and 35 days after heading in late sowings. Total sugar content in grains followed a pattern of linear increase immediately after heading, but it started to decrease around 20 days after heading. On the other hand, starch content continued to increase until maturity. The contents of both components were high in the order of &apos;Olbori&apos; &gt; &apos;Kangbori&apos; &gt; &apos;Suwon 18&apos;, but they did not respond in a regular pattern to different sowings. 1,000 grain weight showed highly significant positive correlation(r=0.767$^{**}$) with the duration of grain growth, but it had negative correlations with the average (r=-0.548$^{**}$) or the sum (r=-0.595$^{**}$) of post-anthesis daily mean temperature.ature.

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Modeling of Sensorineural Hearing Loss for the Evaluation of Digital Hearing Aid Algorithms (디지털 보청기 알고리즘 평가를 위한 감음신경성 난청의 모델링)

  • 김동욱;박영철
    • Journal of Biomedical Engineering Research
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    • v.19 no.1
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    • pp.59-68
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    • 1998
  • Digital hearing aids offer many advantages over conventional analog hearing aids. With the advent of high speed digital signal processing chips, new digital techniques have been introduced to digital hearing aids. In addition, the evaluation of new ideas in hearing aids is necessarily accompanied by intensive subject-based clinical tests which requires much time and cost. In this paper, we present an objective method to evaluate and predict the performance of hearing aid systems without the help of such subject-based tests. In the hearing impairment simulation(HIS) algorithm, a sensorineural hearing impairment medel is established from auditory test data of the impaired subject being simulated. Also, the nonlinear behavior of the loudness recruitment is defined using hearing loss functions generated from the measurements. To transform the natural input sound into the impaired one, a frequency sampling filter is designed. The filter is continuously refreshed with the level-dependent frequency response function provided by the impairment model. To assess the performance, the HIS algorithm was implemented in real-time using a floating-point DSP. Signals processed with the real-time system were presented to normal subjects and their auditory data modified by the system was measured. The sensorineural hearing impairment was simulated and tested. The threshold of hearing and the speech discrimination tests exhibited the efficiency of the system in its use for the hearing impairment simulation. Using the HIS system we evaluated three typical hearing aid algorithms.

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Changes in Chemical Component and Lipid in Tomato Fruits Under the Treatment of Sub-Atmospheric Pressure (감압처리(減壓處理)에 따른 토마토과실(果實)의 주요성분(主要成分) 및 함유지질(含有脂質)의 변화(變化))

  • Kim, Sung-Dal
    • Applied Biological Chemistry
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    • v.23 no.1
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    • pp.31-44
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    • 1980
  • Climacteric phenomon in fruits was delayed ten days on the sub-atmospheric treatment than the normal atmospheric one. Average lipid content in tomatoes sampled was composed of neutral lipid (49%), phospholipid(31%) and glycolipid (13%). Seven constituents each from neutral and phospholipid were seperated through thin-lyaer chromatography. Changes on lipid content during treatments were associated with the climacteric pattern and the advance of maturity; At the climacteric on-set, the fruit color was light pink, and the content of neutral lipid was reduced to minimum. After this stage, this content was reversely increased. The maturity was related with diglyceride and sterol ester in neutral lipid, and phosphatidyl choline and phosphatidyl ethanolamine in phospholipid. The changes of two constituents each on both neutral and phospholipid were reversed each other. It was concluded that changes of lipid content in tomato fruits were closely associated with the advance of fruit maturity and climacteric pattern.

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