• Title/Summary/Keyword: glyceride

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The Contents of Total Lipid and the Component of the Lipid in Porcine Variety Tissue (豚 內臟筋中 總脂質 含量과 構成脂質의 組成)

  • 양철영
    • Journal of the East Asian Society of Dietary Life
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    • v.3 no.1
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    • pp.61-66
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    • 1993
  • The contents of crude fat were remarkably different according to parts in porcine tissues. The most contents of total lipids in variety tissues were neutral lipid, and the nexts were phospholipid and glycolipid, where these of the most composition in liver were phospholipid and the next neutrallipid and glycolipid. The major component of the nonpolar lipids in fresh was triglyceried(58.09∼74.68%) and phospolipid in the polar lipid were phosphatidyl choline(20.14∼45.17%) and phosphatidyl ethanolamine(18.5∼30.14%) and the main component in glycolipid acylsterol glyceride(26.10∼45.15%), respectively.

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Immunological Studies on Ferrbacillus ferrooxidans (Ferrobacillus ferrooxidans에 대한 면역학적 연구)

  • 이강순;장정순;민봉희;이강석
    • Korean Journal of Microbiology
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    • v.11 no.3
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    • pp.115-120
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    • 1973
  • The cell components of an iron-oxidizing F.ferrooxidans were analyzed with heterotrophic E.coli as a control group. The large amounts of total carbohydrate and lipids were represented in F.ferrooxidans and especially the total lipids including total sterol, glyceride, and phospholipid possessed about 4.6% of total dry cell. In amino acids analysis, an unknown metrial was detected in F.ferrooxidans nad it is assumed that material is a ninhydrin-positive and have an association with lipid.

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The Effect of Randomyl Interesterified Triacylglycerols and Vegetable Oils on the Autoxidative Stability (트리아실글리세롤 및 식용유지(食用油脂)의 자동산화(自動酸化) 안정성(安定性)에 대한 에스테르 교환(交換)의 영향)

  • Park, Dong-Ki
    • Journal of the Korean Applied Science and Technology
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    • v.4 no.1
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    • pp.77-81
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    • 1987
  • No difference was observed on autoxidative stability of triacylglycerols before and after randomyl interesterification. It indicate that randomization of the glyceride composition has no significant effect on the autoxidative stability. On the other hand, the autoxidation of randomyl interesterified vegetable oil was accelerated, caused mainly by decrease of tocopherols during the series of interesterification procedures.

한국동물학회 1973년도 춘계연구발표회: 電離放射線이 Sal. typhi의 菌體成分에 미치는 影響

  • 한국동물학회
    • The Korean Journal of Zoology
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    • v.16 no.2
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    • pp.146-148
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    • 1973
  • Sal, typhi, Ty 2 菌體에 電離放射線을 照射하여 放射線 照射時 隋半되는 菌體의 化學的 性狀을 比較 分析하였다. 放射線 2.5 Mrad, 5.0 Mrad 照射로서 酸性 Amino acid는 增加하였으나 中性 및 鹽基性 Amino acid는 若干 減少하였다. 5.0 Mrad 照射로서 菌體의 總 Carbohydrate 量은 49.7%, 總 Nitrogen 量은 44.6%, Lipid 에 있어서는 Sterol 40%, Glyceride 30%, Phospholipid 14%의 減少를 各各 나타내었다. Polyacrylamide gel 電基泳動結果 放射線 照射群에서는 對照群에 比하여 Cell membrane protein의 變性을 認知할 수 있었다.

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Glycosyl glycerides from the stems of 'Baekma' cultivar of Chrysanthemum morifolium (국화 '백마'(Chrysanthemum morifolium) 줄기로부터 glycosyl glyceride 의 분리 및 동정)

  • Oh, Hyun-Ji;Kim, Hyoung-Geun;Pak, Ha-Seung;Baek, Yun-Su;Kwon, Oh-Keun;Shin, Hak-Ki;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.61 no.2
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    • pp.131-134
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    • 2018
  • The stem of Chrysanthemum morifolium, 'Baekma', were repeatedly extracted with 80% aqueous MeOH and the concentrates was partitioned into ethyl acetate (EtOAc), n-butyl alcohol and $H_2O$ fraction. The repeated silica gel and octadecyl silica gel column chromatographies for the EtOAc fractions led to isolation of two glycosyl glycerides. The chemical structures of the compounds were determined as (2S)-1-O-${\beta}-{\text\tiny{D}}$-galactopyranosyl-2,3-dilinoleoylglycerol (1) and (2S)-1-O-${\beta}-{\text\tiny{D}}$-galactopyranosyl-2,3-dipalmitoylglycerol (2) based on spectroscopic data anlyses including nuclear magnetic resonance, mass sperctrometry, and infrared spectrometry and gas chromatography mass spectrometry.

Sustained Release Injectable of Recombinant Bovine Somatotropin in Biodegradable Poly(D,L-lactide-co-glyceride) Microspheres (생분해성 폴리락티드/글리콜리드 미립구를 이용한 재조합 소 성장호르몬(rBST)의 지속성주사제 설계)

  • Jeon, Hong-Ryeol;Lee, Bong-Sang;Kown, Do-W;Yoon, Mi-Kyoung;Jeon, Hyun-Joo;Shin, Taek-Hwan;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.32 no.3
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    • pp.199-207
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    • 2002
  • In order to develop a sustained release formulation of bovine somatotropin (BST), which has been used to increase the body weight of oxen or the milk production of dairy cows, poly(D,L-lactide-co-glyceride)(PLGA) microspheres were made by W/O/W multiple emulsification method and solvent extraction method. Physical properties including particle size, drug entrapment, drug release, protein denaturation, and in vivo body weight increase in rats were characterized. The size of the microspheres was increased as the molecular weight of PLGA increased. When Span 65 and stearic acid during preparation were added, the size was decreased but the amount of surface protein was increased, resulting in a high loading efficiency, with fast release of BST from the microspheres. Aggregation or fragmentation of BST by SDS-PAGE during microsphere preparation and drug release study was not observed. Body weight of Sprague-Dawley's male rats was significantly increased after subcutaneous administrations of BST-loaded PLGA microspheres. There was a good correlation between in vivo weight gain and in vitro release rate of microspheres. PLGA microspheres with a high surface protein ratio could be a good candidate for the sustained delivery of BST.

Composition Ratio Analysis of Transesterification Products of Olive Oil by Using Thin Layer Chromatography and Their Applicability to Cosmetics (올리브 오일의 에스터 교환반응 생성물의 TLC를 이용한 조성비 분석 및 화장품에의 응용가능성 평가)

  • Park, So Hyun;Shin, Hyuk Soo;Kim, A Rang;Jeong, Hyo Jin;Xuan, Song Hua;Hong, In Kee;Lee, Dae Bong;Park, Soo Nam
    • Applied Chemistry for Engineering
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    • v.29 no.3
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    • pp.342-349
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    • 2018
  • In this study, the physicochemical properties, emulsifying capacity, moisture content and cytotoxicity of the composite material produced by transesterification reactions of the olive oil (olive oil esters) were investigated for cosmetic applications. Olive oil esters with short (S) and long (L) reaction times were studied. From the TLC-image analysis, composition ratios of the olive oil esters S were found to be 5.2, 24.1, 46.4, and 21.9% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. Those of the olive oil esters L were 4.1, 24.7, 40.6, and 28.8% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. The iodine value, acid value, saponification value, unsaponified matter, refractive index, and specific gravity were determined and purity tests were also carried out and normalized to establish standards and testing methods for using olive oil esters in cosmetics. To evaluate their emulsifying capacities, the O/W emulsion was prepared without surfactants and the formation of the emulsified particles were confirmed. After 5 days of applying the olive oil esters to human skin, the skin moisture retention was improved by 13.1% from the initial state. For the evaluation of toxicity on human skin cells, the olive oil esters showed 90% or more of the cell viability at $0.2-200{\mu}g/mL$. These results suggested that olive oil esters can be applied as natural/non-toxic ingredients to cosmetics industries.

Studies on the Properties and Frying Performance of Domestic Rice Bran Oil (국산(國産) 미강유(米糠油)의 성상(性狀)과 튀김적성(適性)에 관(關)한 연구(硏究))

  • Kim, Gum-Sik;Yum, Cho-Ae
    • Korean Journal of Food Science and Technology
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    • v.15 no.1
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    • pp.77-89
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    • 1983
  • Properties and frying performance of domestic rice bran oil were studied. For this purpose, the analyses on the following characteristics were performed and their comparison with the characteristics of soybean oil was made: fatty acid composition, glycerides, composition, acid value, specific gravity, color, petroleum ether insoluble oxidized fatty acid, viscosity, smoke point, foam test, and water solubility. The results were as follow: 1) Fatty acid composition of domestic rice bran oil by gas chromatography was same as perviously reported, and similar to the fatty acid composition of the rice bran oil in foreign countries. Also the glyceride composition of domestic rice bran oil was analyzed by the methods of TLC, column chromatography, and high performance liquid chromatography. The results were monoglyceride ranged from one to four percent; diglyceride 30-30% and triglyceride 66-80%. Consequently, the composition was proven completely different from that of the other edible oils. 2) The high contents of mono-glyceride in rice bran oil resulted in high values of specific gravity and water-solubility respectively. However, high contents mono glyceride and diglyceride indicated little affection to changes of acid value, color, petroleum ether insoluble oxidized fatty acid, and smoke point on frying. 3) Because of low contents of linoleic acid, domestic rice bran oil was estimated stable on frying, whereas soybean oil was easily polymerized. 4) A serious foaming and low smoke point on Frying in domestic rice bran oil were caused by its impurities. It seems that monoglyceride and diglyceride had little relation with foaming and smoke point. 5) Oils with serious foaming and low smoke point on frying caused a substantial quality loss in terms of flavor and appearance of fried materials.

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Detection and Determination of Lipase Activity

  • Lee, Seoung-Yong;Rhee, Joon-Shick
    • Journal of Microbiology and Biotechnology
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    • v.4 no.2
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    • pp.85-94
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    • 1994
  • Lipase (triacylglycerol hydrolase, EC 3.1.1.3) is able to catalyze the hydrolysis of ester bonds of triacylglycerols at the interface between aqueous phase and organic phase containing substrate. With the rapid development of lipid biotechnology, lipase-catalyzed hydrolysis of lipids has a great concern from the industrial point of view. Owing to the reversible nature of the lipase, the reactions are also applied for glyceride synthesis, interesterification and resolution of racemic mixtures into optically active alcohols or acids. For all applications of the lipases, a reliable method for the determination of enzyme activity is required. Precise quantitative determination of its activity is essential as the basis of research and development of the bioprocess involving the enzyme. This article reviews the existing literature on the detection and determination of lipase activity from microbial, mammalian and plant sources.

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The distribution of fatty acids in the position of glycerides hydrolyzed from fish oil by lipase

  • Kim, Yong-Min;Hu, Hong-Bo;Hur, Byung-Ki
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.505-508
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    • 2000
  • In order to determine the position and the content of fatty acids attached to glycerides and the migration degree of fatty acids in the migration reaction, hydrolysis of fish oil was carried out with lipolase-100T derived from Aspergillus oryzae. The content of fatty acids in the glyceride mixture was analyzed and compared with that of fish oil. The amounts of fatty acid in 2-position and the migration degree of the fatty acid in 2,3-DG (diglyceride) and 2-MG (monoglyceride) were calculated. The results showed that approximately 95% (w/w) of DHA (docosahexaenoic acid) and 65% of EPA (eicosapentaenoic acid) was attached to the 2-position of glycerides in the fish oil. Approximately 87% (w/w) of DHA and 75% of EPA remained in 2,3-DG and 88% of DHA and 65% of EPA in 2-MG were not involved in the migration reaction.

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