• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.93-100
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• 2015

The objective of this study was to determine the effectiveness of rapeseed meal in controlling soil nematode. Two different rapeseed meals (Jeju local rape varieties and 'Sunmang' variety) were mixed with the soil to control nematodes environmentally. When soil physical properties in the rapeseed meal-mixed soils were analyzed, OM (organic matter), $P_2O_5$, Ca, Mg, CEC (Cation Exchange Capacity) value increased. Especially, the level of OM was 3-fold higher than control soil. Glucosinolate content of rapeseed meal was higher in Jeju local rape varieties than 'Sunmang' variety. The major components of glucosinolates were consisted of progoitrin, gluconapin, glucobrassiaca napin, and sinigrin. These components were likely to be involved in reducing nematode density.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.315-322
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• 2013

BACKGROUND: This study was performed for the identification and quantification of glucosinolate (GSL) contents in seven varieties of rapeseed (Brassica napus L.) sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates (GSLs) were desulfated by treating with aryl sulfatase and purified using diethylaminoethyl sepharose (DEAE) anion exchange column. Individual GSLs were quantified using high-performance liquid chromatography (HPLC) with electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Eleven GSLs including six aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, and glucobrassicanapin), four indolyl (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) and one aromatic (gluconasturtiin) were identified based on the fragmentation patterns of MS spectrum. Aliphatic GSLs were noted as the predominant group with average 85.2% of the total contents. The most abundant GSLs were progoitrin which was ranged at $8.14-118.68{\mu}mol/g$ dry weight (DW). The highest total GSL amounts were documented in 'Hanra' ($146.02{\mu}mol/g$ DW) under light condition and 'Mokpo No. 68' ($86.67{\mu}mol/g$ DW) in dark condition, whereas the lowest was in 'Tamra' (30.13 and $14.50{\mu}mol/g$ DW) in both conditions. The sum of aliphatic GSLs attributed > 80% in all varieties, except 'Tamra' (67.7% and 64.9% in dark and light conditions, respectively) in the total GSL accumulation. Indolyl GSLs were ranged $2.41-15.73{\mu}mol/g$ DW, accounted 2.78-33.6% of the total GSLs in rapeseed varieties. CONCLUSION(S): These results provide valuable information regarding potential beneficial GSL contents individually. This study attempts to contribute to knowledge of the nutritional properties of the different varieties of rapeseed plants. These results may be useful for the evaluation of dietary information.

• Korean Journal of Agricultural Science
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• v.41 no.2
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• pp.125-133
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• 2014

The variation of glucosinolates (GSLs) in Chinese cabbage ('Sinhongssam') (Brassica rapa L. spp. pekinensis) cultivated under lights to control plant growth conditions was evaluated at different development stages. Under experimental conditions in plant factory system, plant growth conditions including light, temperature, and nutrients were designed to enhance GSLs. The variation of glucosinolates (GSLs) in Chinese cabbage ('Sinhongssam') (Brassica rapa L. spp. pekinensis) cultivated under lights to control plant growth conditions was evaluated at different development stages. Under experimental conditions in plant factory system, plant growth conditions including light, temperature, and nutrients were designed to enhance GSLs. The contents of GSLs were quantified in Chinese cabbage according to different light sources (Red+White, RW; Red+Blue+White, RBW, Fluorescence lamp, FL) at development stages (28, 42, and 56 days after sowing, DAS) using HPLC. Nine GSLs including five aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin) three indolyl (glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin), and one aromatic (gluconasturtiin) GSLs were identified based on peak retention time in previous results of our laboratory. GSL contents were higher in RBW (36.55) and lower in FL ($15.24{\mu}mol/g/\;DW$). Results revealed that GSL contents were higher under controlled photoperiods (20/4 h) ($58.35{\mu}mol/g\;DW$) and controlled light intensity ($160{\mu}mol/m^2/s$) ($34.02{\mu}mol/g\;DW$), respectively. Lower amount of progoitrin and comparatively higher amount of glucobrassicin and gluconasturtiin was noted in Chinese cabbage cultivated under FL light (2.38, 9.82, and 2.10) at 42 DAS, photoperiod 20/4 h (3.16, 2.52, and 1.30) at 28 DAS, and light intensity at $130{\mu}mol/m^2/s$ (2.28, 2.24, and $1.51{\mu}mol/g\;DW$) at 42 DAS. Therefore FL light, photoperiod (20/4 h), and light intensity ($130{\mu}mol/m^2/s$) were considered as most suitable for the enhancement of GSLs in Chinese cabbage.