• Journal of the Korean Society of Food Science and Nutrition
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• v.5 no.1
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• pp.25-30
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• 1976

In this investigation, the physico-chemical properties of Korean cattles milk and Holstein cows milk are studied. The results of the milk -protein of F-1, F-2, and F-4 hybrid are interested in this studied. 1. The physico-chemical properties of Korean cattle and Holstein cows milk is summarized at Table 1, Table 2. 2. The electrophoretic milk protein fraction of Korean cattle is identified : Lactoalbumin 1.74mg%. Alpha-1 globulin 1.19mg%, Alpha-2 globulin 0.68mg%, Beta-globulin 2.57mg%, Gamma-globulin 0.93mg%. 3. The electrophoretic milk protein fraction of Holstein cow : Lactoalbumin 1.23mg%, Alpha-1 globulin 0.93mg%, Alpha-2 globulin 0.86mg%, Bata-globulin 2.56mg%, Gamma-globulin 1.74mg% have been identified. 4. The electrophoretic milk protein fraction of hybrid F-1, F-2, and F-4 between Korean cattle(female) and Holstein(male) is obtained the following : F-1 hybrid : Lactoalbumin 1.38mg%, Alpha-1 globulin 1.58mg%, Alpa-2 globulin 2.46mg%, Beta-globulin 1.39mg%. F-2 hybrid : Lactoalbumin 1.57mg%, Alpha-1 globulin 1.74mg%, Alpha-2 globulin 2.19mg%, Beta-globulin 1.74mg%. F-4 hybrid : Lactoalbumin 2.46mg%, Alpha-1 globulin 1. 24mg%, Alpha-2 globulin 1.57㎎%, Beta-globulin 1.74mg%.

• The Korean Journal of Food And Nutrition
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• v.2 no.1
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• pp.18-26
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• 1989

The objective of this study was to improved the limited functional characteristics of mungbean whole globulin. The mungbean whole globulin was acylated with succinic and acetic anhydride, and the functional properties of acylated protein were investigated, The results obtained ware as follows. 1. The UV-absorption spectra of acylated whole globulins with that of the succinylated 74% whole globulin as large blue shift of the absorption maximum and minimum wavelength from 275 nm to 269 nm, respectively. 2. The mobility of acylated whole globulin were increased on PAGE pattern, and degree of mobility was particularly remarkable in case of succinylation, 3. The water absorption capacity of whole globulin was increased by acylation. The most increased rate of whole globulin was 174,02% from succinylated 74%. The oil absorption capacity of whole globulin was increased by acylation The most increased rate of whole globulin was 165.41% from acetylated 81.77%. 4. The bulk density of whole globulin was decreased by acylation. and the greater the extent of acylation, the smaller the bulk density. 5. The foaming capacity and stability of whole globulin was increased by acylation, and remarkably high in 74% succinylated whole globulin. In contrast, however, the foaming capacity and stability of native and acylated whole globulin were decreased by heat treatment.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.299-304
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• 2000

The activated immune systems with lipopolysaccharide(LPS) were evaluates by ${\gamma}$- globulin levels in domestic cats. Especially, cats were in a stress condition, which was unfamiliar environmental change. Effect of continuous unfamiliar environmental stressor was a diminution of production of serum total globulin auld ${\gamma}$- globulin on feline infectious peritonitis(FIP) vaccination. The ${\gamma}$-globulin production on FIP vaccination more increased in LPS treated 77ups than non-LPS treated group. Also. the ${\gamma}$-globulin production on FIP vaccination was maintained in LPS treated group under stress condition. These results imply that that the ${\gamma}$-globulin can be produced under a stress condition by mitogen like as LPS.

• Korean Journal of Clinical Laboratory Science
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• v.37 no.2
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• pp.61-64
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• 2005

We compared the serum protein electrophoresis patterns in sera of diabetes patients (n=166) which showed more than 150 mg/dL of glucose. The concentrations of total protein, albumin, ${\alpha}1$-globulin, ${\alpha}2$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin in sera of patients were $6.3{\pm}1.2g/dL$, $40.3{\pm}6.4%$, $4.9{\pm}2.3%$, $13.5{\pm}3.8%$, $15.5{\pm}3.9%$, and $25.9{\pm}5.8%$, respectively. The frequency of patients showed lower levels of total protein, albumin, ${\alpha}1$-globulin were 48.5%, 96.4%, and 33.7%. The frequency of patients showed higher levels of ${\alpha}2$-globulin, ${\beta}$-globulin, and ${\gamma}$-globulin were 56.6%, 47.0%, and 90.4%, respectively. This data suggests that the high concentration of glucose in blood may damage the kidney and liver. Also, the decrease of albumin and ${\alpha}1$-globulin and increase of ${\alpha}2$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin shows that the concentration of glucose in blood will have an effect on the variation of serum protein electrophoretic patterns.

• Korean Journal of Veterinary Research
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• v.11 no.2
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• pp.145-148
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• 1971

Serum Samples from adult of Korean cattles including 40 females and 20 males were analyzed by sodium salt precipitation and colorimetric method in the purpose of the determination of total serum protein, albumin, globulin, ${\alpha}$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin. The results obtained arc summarized as follows: 1. Mean value of total serum protein showed a slight variation from 7.6%, and its regional and sex differences were not found to be significant. 2. Contents of albumin in serum showed lower level than that of globulin as low level of A/G ratio 0.4 in proportion. 3. Contents of Serum ${\alpha}$-globulin showed 1.4w/v% and $1.51{\pm}0.46$w/v% in each group of female, and $1.31{\pm}0.26$w/v%, in the group of male. 4. Contents of serum ${\beta}$-globulin showed 1.74w/v%, 1.95w/v%, in each group of female, and 1.82w/v% in the group of male. 5. Contents of serum ${\gamma}$-globulin showed 2.32w/v%, 2.30w/v% in each group of female, and 2.30w/v%, in the group of male.

• Food Science and Biotechnology
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• v.15 no.1
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• pp.128-132
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• 2006

This study was undertaken to investigate the effect of peptic and chymotryptic hydrolyses of 7S globulin, the major allergen of soybean protein, on its allergenicity, as measured by enzyme linked immunosorbent assay (ELISA), and to identify the allergenic hydrolyzed fragments of 7S globulin using SDS-PAGE. When 7S globulin was hydrolyzed by pepsin, the allergenicity was reduced by over 50%. However, the allergenicity of 7S globulin reduced by peptic hydrolysis was recovered in the sera from 5 out of 10 patients following sequential chymotryptic hydrolysis. Two fragments, with molecular weights 20-25 and 13-16 kDa, among the hydrolysate of 7S globulin by sequential pepsin and chymotrypsin showed reactivity with sera from 10 soybean-allergenic patients. As a result of the theoretical hydrolyses of ${\beta}$-conglycinin, which is a major protein of 7S globulin, it is suggested that the 20-25 kDa fragments were the fragments of the ${\alpha}$-subunit of ${\beta}$'-conglycinin and that the 10-16 kDa fragments were from the ${\alpha}$'-subunit.

• Biomedical Science Letters
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• v.16 no.1
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• pp.19-24
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• 2010

We analyzed and compared the concentration of total cholesterol (CHOL), high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol and triglyceride (TG) in serum and the serum protein electrophoresis fractions of thyroid disease patients. In comparison with the average of reference, our data showed that the average concentration of CHOL, LDL cholesterol and TG in hyperthyroidism patients were decreased significantly, but HDL cholesterol was increased significantly. In hypothyroidism patients, CHOL, HDL cholesterol, LDL cholesterol and TG were all increased significantly. In comparison of the concentration of lipids in each patient to reference range, 28.3% of hyperthyroidism patients showed abnormally low level of total cholesterol. In the patients with hypothyroidism, the percentage of patients showed abnormally high level of CHOL, HDL cholesterol, LDL cholesterol and TG were 37.7%, 10%, 68.8% and 49.1%, respectively. In our studies of serum protein electrophoresis, the average of ${\alpha}_2$-globulin and $\gamma$-globulin in hyperthyroidism patients were increased and $\beta$-globulin was decreased significantly. In hypothyroidism patients, the average of $\gamma$-globulin was increased and $\beta$-globulin was decreased significantly. In comparison of protein fractions of each patient to reference range, 38.3% and 50.0% of hyperthyroidism patients showed abnormally high levels of ${\alpha}_2$-globulin and $\gamma$-globulin, but 73.3% of patients showed abnormally low level of $\beta$-globulin. In hypothyroidism patients, 70.4% of patients were abnormally decreased in $\beta$-globulin and 63.9% of patients were abnormally increased in $\gamma$-globulin. These data suggest that the concentrations of CHOL, HDL cholesterol, LDL cholesterol and TG are not critical data for clinical interpretation of hyperthyroidism, but the levels of them are useful for interpretation of hypothyroidism patients. Our results of serum protein electrophoresis suggest that the concentration of serum protein electrophoresis fractions can be useful to understand the thyroid disease.

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.219-226
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• 1992

This study was performed to observe the therapeutic effects of interferon-gamma ($IFN-{\gamma}$) and gamma-globulin (${\gamma}-globulin$) in experimental Pneumocystis carinii pneumonia of immune suppressed mice. After 9 weeks, trimethoprim-sulfamethoxaBole(TMP-SMZ; 10~50 mg/mouse/day), mouse $IFN-{\gamma}(5{\times}10^4$ units/mouse/day) and mouse ${\gamma}-globulin$(20 mg/mouse/day) were administered to the mice for 3 weeks by the experimental group. The therapeutic efficacy was evaluated by body weights, histopatholo단ic and electron microscopic findings of the lungs, and number of p. carinii cysts by Gomori's methenamine silver stain. Body weights of the mice were significantly increased in the group of combination therapy of TMP-SMZ with $IFN-{\gamma}{\;}or{\;}{\gamma}-globulin$, and in the group of TMP- SMZ treatment(p<0.05), however, little effect was found in the group of T-globulin alone. Histopathologic 6ndings of p. carinii pneumonia were much improved in the group of combination therapy of TMP-SMZ with $IFN-{\gamma}$. Treatment with either TMP-SMZ or $IFN-{\gamma}$ significantly reduced the number of cysts in the p. carinii pneumonia, but {\gamma}-globulin alone was ineffective. In electron microscopic findings of p. carinii pneumonia, the number of trophozoites and cysts were reduced by treatment with either TMP-SMZ or $IFN-{\gamma}$, and most of the cysts were empty or containing one or two intracystic bodies. The present results suggested, that combination therapy of TMP-SMZ with $IFN-{\gamma}$ had synergistic effects in treatment of P carinii pneumonia in experi- mental mice.

• Korean Journal of Food Science and Technology
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• v.12 no.1
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• pp.77-81
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• 1980

The effect of dietary vegetable oils, sesame, perilla, soybean and rice bran oils, on the blood cholesterol level of rabbit was examined using isocaloric and isonitrogenous diets. The gain in body weight, liver weight, serum and liver cholesterol levels, globulin, blood sugar and acid phosphatase activity in relation to cholesterol level were studied. The results are summarized as follows : 1. The gain in body weight (g/day) of rabbit was 16.3 for control, 15.3 for A, 18.3 for 15.3 for A, 18.3 for B, 15.3 for C and 18.1 for D groups. 2. Liver weight of the control A, B, C and D groups were 30.35, 37.25, 38.25, 31.05 and 39.54, respectively. 3. Serum cholesterol levels (mg/100 ml serum) of the control, A, B, C, and D groups were 71.5, 112.0, 110, 93 and 96 respectively. 4. Liver cholesterol levels (mg/100 ml liver fat) of the control, A, B, C and D groups were 255, 292, 255, 317 and 195 respectively. 5. The contents of alpha-1-globulin for control was 0.60 %, for C, 0.35 % and for D groups, 0.32% of total globulin. The content of alpha-2-globulin for control was 0.68 % of total globulin and for D, 0.26 % of total globulin. 6. The contents of blood glucose (mg/100 ml) of the control, A, B, C and D groups were 40.34, 22.37, 77.0, 28.6 and 34.1 respectively. 7. Acid phosphatase of the control, A, B, C, and D groups were 3.95, 4.52, 3.98, 4.55 and 4.53 nM/hr/l serum respectively. 8. According to the regression and correlation in coefficient in blood components of rabbit, there were positive correlations between serum cholesterol and alpha-1-globulin, and between liver cholesterol and gamma-globulin.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.26-32
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• 1977

The multiple 7S globulins composed of two fractions (A and B) in the electrophoresis with Davis' method were isolated at different stages of the soybean seed development. Electrophoresis of their subunits liberated in PAWU solvent [phenol-acetic acid-water (2 : 1 : 1) solution plus 5M urea] yielded 4 major bands. Observation of both the electrophoretic bands of the multiple 7S fractions(7S-A and 7S-B) and those of their subunits was suggestive of a similarity of the subunit pattern between two 7S fractions. The two fractions in multiple 7S globulins were isolated with DEAE-Sephadex A-50 column$(2.0{\sim}100cm)$ chromatography. They were separated into 2 fractions in a linear gradient concentration of 0.28 to 0.40M NaCl with phosphate buffer (pH 7.8) containing 10mM ${\beta}-mercaptoethanol$(ME). The isolated protein was dissociated into subunits with two different solvent systems; in PAWU solvent and in Tris-HCl buffer(pH 8.0) containing 1% sodium dodecyl sulfate (SDS) and 40mM ME. The dissociated subunits were subjected to electrophoresis in PAWU-treated 7.5% acrylamide gel and in 1% SDS-treated 5.6% acrylamide gel. In PAWU gel electrophoresis, total 7S globulin was separated into 5 major bands, two of which were occupied in common by two 7S fractions(7S-A and 7S-B). In SDS gel electrophoresis, total 7S globulin was separated into 7 major bands, three of which were overlapped with the subunit of the two 7S fractions. The above results alluded us to the presence of a common and/or similar subunit between the multiple 7S globulins.