• Food Science of Animal Resources
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• v.39 no.2
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• pp.324-331
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• 2019

The objective of this study was to evaluate physicochemical and sensory properties, the texture profile, and antioxidant activity of ginseng extract-supplemented quark cheese as a new cheese product intended to improve public health. After addition of less than 1.0% ginseng extract, the moisture content of quark significantly decreased, while fat and protein levels increased, although microbial counts and lactose and ash contents were not affected significantly (p<0.05). In terms of color, $L^*$ values decreased significantly with increasing concentration of ginseng extract, while $a^*$ values increased significantly (p<0.05). The results of texture profiling showed that cohesiveness and springiness were unaffected, whereas hardness, gumminess, and chewiness increased significantly. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical-scavenging activities of the cheese fortified with 0%, 0.5%, or 1.0% of the ginseng extract were $4.22%{\pm}0.12%$, $20.14%{\pm}1.34%$, and $56.32%{\pm}1.54%$, respectively. The results of sensory analysis indicated that bitterness, ginseng odor, and aftertaste significantly improved with increasing concentration of ginseng extract (p<0.05). However, there was no significant difference in the overall quality attributes of quark cheese between the no-supplement control and samples with less than 0.5% of the ginseng extract (p>0.05), suggesting that these products could help to promote public health as functional foods.

• Journal of Ginseng Research
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• v.48 no.1
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• pp.103-111
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• 2024

Background: Ginseng (Panax ginseng Mayer) is an important natural medicine. However, a long culture period and challenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainable medicinal supply, research on the association between the transplantation and chaining of metabolic networks, especially the regulation of ginsenoside biosynthetic pathways, is limited. Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurements to evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of genetic alterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtained by MapMan, we constructed a metabolic profile of transplantation Ginseng. Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but following transplantation, the ginsenoside content increased significantly from 6-8 years (in the wild). Glycerolipid metabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids and lipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathways during transplantation Ginseng growth. Conclusion: This study expands our understanding of metabolic network features and the accumulation of specific compounds during different growth stages of this perennial herbaceous plant when growing in transplantation mode. The findings provide a basis for selecting the optimal transplanting time.

• Journal of the Korean Society of Tobacco Science
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• v.12 no.2
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• pp.91-101
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• 1990

A field experiment was conducted to find out the effects of field productivity, variety and nitrogen rate on the yield, quality, chemical constituents and physical properties of burley cured leaf in three field with different productivity(Degree of field productivity: A ; high, B ; medium, C : low) during successive two years(1988~89). The yield and quality were remarkably lowered when nitrogen fertilizer being applied much in low productive field. As compared with Burley 21, KB101 showed high yield, particularly the yield of KB101 in low productive field was relatively high. The effect of nitrogen rate on the yield was somewhat different according to field productivity and production year. When the nitrogen fertilizer being applied above 22.5kg/10a, the added nitrogen had no effect on the yield. Total nitrogen content of cured leaf grown in low productive field was high while total alkaloid was low, therefore total alkaloid/total nitrogen ratio was remarkably low. The lightness, red and yellow color of cured leaf grown in low productive field was remarkably low. As compared with Burley 21, the contents of total alkaloid and total nitrogen and shatter resistance index of cured leaf was somewhat low, while the filling power, lightness, red and yellow color were slightly high. Total nitrogen content of cured leaf was increased remarkably by nitrogen addition, but total alkaloid was not increased though the nitrogen fertilizer being applied above 22.5kg/10a. The filling power and shatter resistance index of cured leaf grown in high nitrogen plot, and the lightness and yellow color were low while the red color was relatively high. It comes into question that the visual quality being increased as well as increment of yield and nitrogenous compounds by nitrogen addition in high productive field. In low productive field, it is considerable that nitrogen addition for high yield should be prohibited because it causes the decrement of yield and quality, on the contrary.

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.291-295
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• 2007

This study was conducted to investigate the optimum shading material of rain shelter in Panax ginseng C.A. Meyer. Results obtained from our experiment can be summarized as follows. From photosynthetically active radiation (PAR) as shade materials on sunny day, temperature under rain shelter was low in comparison with that under polyethylene net. It was ranged from 3 to $5^{\circ}C$ Survival rate of 3,4, and 5 year-old ginsengs under shade materials of rain shelter was lower than that under polyethylene net. Root fresh weight in ginseng grown under rain shelter was increased into about 5.0g in 3 year-old, 10g in 4 year-old and 8g in 5 year-old ginsengs. From investigating root grade of 3, 4, and 5 year-old ginsengs, we confirmed that rain shelter was more effective than the other as shading materials. Root size of 3, 4, 5 year-old ginsengs grown under rain shelter is distributed in bigger size than that grown under polyethylene net. Also, in the case of saponin contents, the ginseng grown under the shade material of rain shelter was higher than that under polyethylene net.

• Food Science and Preservation
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• v.16 no.2
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• pp.192-197
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• 2009

This study investigated the quality characteristics of Chun-dubu(whole soybean curd) to which ginseng powder was added. The overall composition of the material was moisture $80.11{\pm}0.32%$, crude protein $6.04{\pm}0.007%$, crude fat $5.85{\pm}0.007%$, and crude ash $1.06{\pm}0.014%$(all w/w). The total microorganism count was 3.57 log CFU/g, whereas that of control Chun-dubu was 4.82 log CFU/g after 15 days of storage at $5^{\circ}C$. No coliforms were detected in Chun-dubu with added ginseng powder, whereas the control Chun-dubu coliform count was 3.52 log CFU/g after 15 days of storage at $5^{\circ}C$. When textural properties were considered, all of hardness, springiness, and chewiness rose during storage at $5^{\circ}C$. The sensory characteristics of Chun-dubu with added ginseng powder rated higher(from 5.80 to 6.10) than those of control Chun-dubu.

• Journal of Ginseng Research
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• v.35 no.4
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• pp.487-496
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• 2011

Good manufacturing practice (GMP)-based quality control is an integral component of the common technical document, a formal documentation process for applying a marketing authorization holder to those countries where ginseng is classified as a medicine. In addition, authentication of the physico-chemical properties of ginsenoside reference materials, and qualitative and quantitative batch analytical data based on validated analytical procedures are prerequisites for certifying GMP. Therefore, the aim of this study was to propose an authentication process for isolated ginsenosides $Rb_1$ and $Rg_1$ as reference materials (RM) and for these compounds to be designated as RMs for ginseng preparations throughout the world. Ginsenoside $Rb_1$ and $Rg_1$ were isolated by Diaion HP-20 adsorption chromatography, silica gel flash chromatography, recrystallization, and preparative HPLC. HPLC fractions corresponding to those two ginsenosides were recrystallized in appropriate solvents for the analysis of physico-chemical properties. Documentation of the isolated ginsenosides was made according to the method proposed by Gaedcke and Steinhoff. The ginsenosides were subjected to analyses of their general characteristics, identification, purity, content quantitation, and mass balance tests. The isolated ginsenosides were proven to be a single compound when analyzed by three different HPLC systems. Also, the water content was found to be 0.940% for $Rb_1$ and 0.485% for $Rg_1$, meaning that the net mass balance for ginsenoside $Rb_1$ and $Rg_1$ were 99.060% and 99.515%, respectively. From these results, we could assess and propose a full spectrum of physicochemical properties for the ginsenosides $Rb_1$ and $Rg_1$ as standard reference materials for GMP-based quality control.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.388-395
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• 2000

This study was performed to examine the effects of infrared (IR)/heated-air combination drying on some quality attributes of Korean white ginsengs. Ginseng roots were dried in a dryer where both the far infrared ray and heated-air are available as drying energy sources. Diametral shrinkage, external color, total saponin content, and ginsenosides and free sugar composition of the IR/heated-air combination dried ginsengs were measured and compared with those of commercial white ginseng products. The external color became lower in lightness and higher in saturation as the IR radiating plate temperature increased. IR/heated-air combination dried white ginsengs at IR plate temperature of 100$^{\circ}C$ was comparable to the commercial white ginseng products in color characteristics. Diametral shrinkage ratios ranged from 20 to 36% and appeared to be independent on the different drying methods. No definite evidence could be found whether the IR/heated-air combination drying and the conventional. hot-air drying practice resulted in white ginsengs having different ginsenoside contents and compositions. No conclusion could be made on whether the various drying treatments used in the study had effects on the free sugar contents and compositions of white ginsengs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.19-23
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• 1986

To get the basic information about ginseng seed production, yield of seed and seed quality in 27 fields were investigated. Yield of seed ranged from 4 to 10 litres per 100 kan (180cm x 90cm). Ratio of under 4mm seed is 12%, 66% for 4 to 5mm and 22% for over 5mm. Seed weight and ratio of over 4mm seed showed the decreasing tendency with the increase of seed yield per unit area. The seed yield harvested from the selected mother plants was lower than that from all plants, but seed weight and ratio of over 4mm seed were high in fields using the selected mother plants. It showed a positive correlation between length and width of seed, but no correlation between thickness and length or width. Optimum yield for high quality seed appeared under the 7 to 8 litres per 100 kan.

• Food Science and Preservation
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• v.14 no.4
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• pp.369-377
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• 2007

This study investigated the effect of ginseng products on the baking properties of white breads. Flour was substituted by ginseng products (ginseng at levels 2, 4, 6, and 8%, all w/w, of flour levels). Both ginseng powder (GP) and red ginseng powder (RGP) were used. Similarly, red ginseng extract (RGE) was substituted at levels of 1, 2, 3, and 4% (all w/w) of flour. The physical properties of each dough were assessed using farinograms, extensograms, and amylograms. Water absorption increased as the proportions of ginseng products rose. The dough surface areas and R/E (resistance/extensibility) values decreased, as did peak viscosities, at the proportions of ginseng products increased. The pH values during fermentation decreased as the proportions of ginseng products increased. The fermentation power of dough with GP was lower than that of control, and higher than that of dough with RGP or RGE, but the addition of ginseng products beyond certain levels weakened the gas retention power: The specific loaf volumes of breads with 2% GP were the highest at 5.41 mL/g. In breads with RGE, the specificloaf volume increased from 5.52 mL/g to 5.82 mL/g as RGE levels rose from 0% to 4%. Hardness increased with rising GP and RGP levels in breads with GP and RGP, but decreased with RGE levels in breads with RGE. The moisture contents of breads during storage tended to be higher than control in breads with ginseng products. Lightness increased with addition of GP and decreased upon addition of RGP or RGE, while redness and yellowness increased after addition of any ginseng products. In sensory evaluation tests, the sensory scores for texture, color, mouth feel, and overall acceptability, were high, when any ginseng product (GP, RGP or RGE) was present at 2%. Of these breads, the bread with 2% RGE attained the highest sensory score.

• Journal of Ginseng Research
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• v.43 no.4
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• pp.508-516
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• 2019

Background: Ginsenosides are not only the principal bioactive components but also the important indexes to the quality assessment of Panax ginseng Meyer. Their contents in cultivated ginseng vary with the growth environment and age. The present study aimed at evaluating the significant difference between 36 cultivated ginseng of different cultivation areas and ages based on the simultaneously determined contents of 14 ginsenosides. Methods: A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometer (MS) method was developed and used in the multiple reaction-monitoring (MRM) mode (HPLC-MRM/MS) for the quantitative analysis of ginsenosides. Multivariate statistical analysis, such as principal component analysis and partial least squares-discriminant analysis, was applied to discriminate ginseng samples of various cultivation areas and ages and to discover the differentially accumulated ginsenoside markers. Results: The developed HPLC-MRM/MS method was validated to be precise, accurate, stable, sensitive, and repeatable for the simultaneous determination of 14 ginsenosides. It was found that the 3- and 5-yr-old ginseng samples were differentiated distinctly by all means of multivariate statistical analysis, whereas the 4-yr-old samples exhibited similarity to either 3- or 5-yr-old samples in the contents of ginsenosides. Among the 14 detected ginsenosides, Rg1, Rb1, Rb2, Rc, 20(S)-Rf, 20(S)-Rh1, and Rb3 were identified as potential markers for the differentiation of cultivation ages. In addition, the 5-yr-old samples were able to be classified in cultivation area based on the contents of ginsenosides, whereas the 3- and 4-yr-old samples showed little differences in cultivation area. Conclusion: This study demonstrated that the HPLC-MRM/MS method combined with multivariate statistical analysis provides deep insight into the accumulation characteristics of ginsenosides and could be used to differentiate ginseng that are cultivated in different areas and ages.