• Journal of Plant Biology
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• 제19권4호
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• pp.107-110
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• 1976

This study was carried out to investigate lead contamination in the leaves of roadside trees and the cause of yellow-brown symptom of leaves in Jinju and Daegu city. After the leaves were collected and dried in 105$^{\circ}C$ drying oven from September to October in 1974, the samples were used for analysis of the lead contents in 1976. The roadside trees in the two cities were mostly the four kinds of Ginkgo biloba L., Thuja orientalis L., Platanus orientalis L. var. acerifolia AIT. and Prunus pseudo-cerasus $LINDL{\times}Prunus$ subhirtella MIQ. The lead contents in the leaves of roadside trees in Daegu city was higher than that of Jinju city. The lead contamination of the evergreen trees of roadside was higher than that of the deciduous trees. In Jinju city the site of the highest contents of lead in the leaves was revealed with Jinju station(0.73ppm), the next site was revealed with the road(0.16ppm) between Namgang bridge and Jinju middle school, and the lowest site was generally beside the roads of suburbs (0.07ppm). Because of the low contents of lead in the leaves, it was found that the yellow-brown symptoms of leaves were not caused by the lead toxicity.

• 대한의생명과학회지
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• 제28권2호
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• pp.92-100
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• 2022

Ginkgo biloba Leaf Extract (GBE) is an extract from leaves of the Ginkgo biloba tree, widely used as a health supplement. GBE can inhibit the proliferation of several types of tumor cell. Although it is known to have anti-cancer effects in breast cancer and skin cancer, research related to gastric cancer is still insufficient. Based on results showing anti-cancer effects on solid cancer, we aimed to determine whether GBE has similar effects on gastric cancer. In this study, the anti-cancer effect of GBE in gastric adenocarcinoma was investigated by confirming the cell proliferation inhibitory effect of AGS cells. We also evaluated whether GBE regulates expression of the tumor suppressor protein p53 and Rb. GBE has apoptotic effects on AGS cells that were confirmed by changes in anti-apoptosis protein Bcl-2, Bcl-xl and pro-apoptosis protein Bax levels. Wound healing and cell migration were also decreased by treatment with GBE. Furthermore, we verified the effects of GBE on mitogenic signaling by investigating AKT target gene expression levels and revealed downregulated Sod2 and Bcl6 expression. We also confirmed that expression of inflammation-related genes decreased in a time-dependent manner. These results indicate that GBE has an anti-cancer effect on human gastric cancer cell lines. Further research on the mechanism of the anti-cancer effect will serve as basic data for possible anti-cancer drug development.

• 대한의생명과학회지
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• 제23권2호
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• pp.80-88
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• 2017

Ginkgo biloba extract (EGb 761) is a standardized extract of Ginkgo biloba leaves and has anti- atherosclerosis properties. Many patients with atherosclerosis disorders take Ginkgo biloba extracts to supplement current therapy. In addition, normal healthy individuals also take Ginkgo biloba extracts for prophylactic purposes. However, it is unknown whether supplementation of Gingko biloba extracts in healthy individuals offer a benefit. In this study, we assessed whether EGb 761 could provide beneficial effects on serum cholesterol levels in normal mice. Wild-type C56Bl/6 mice were orally administered EGb 761 at 25 mg/kg (Group 3) or 50 mg/kg (Group 4) every other day for 40 days. We found that the serum levels of HDL-cholesterol (HDL-C) were significantly increased in EGb 761 and lovastatin treated groups. Treatment with EGb 761 and lovastatin resulted in reduced serum total cholesterol and LDL-cholesterol (LDL-C) compared to control group. Serum lecithin cholesterol acyltransferase (LCAT) levels were higher in EGb 761 and lovastatin treated group compared to the control group. However, no difference was observed in serum APO A-I levels between the control group and treatment group. These results suggest that EGb 761 can increase HDL-C resulting in increased serum LCAT levels.

• 한국융합학회논문지
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• 제11권8호
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• pp.223-228
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• 2020

아름다운 헤어스타일을 위하여 건강한 모발을 꾸준히 관리해야 할 필요성이 강하게 대두(擡頭)되고 있으며, 시대적 요구에 따라 인체에 무해(無害)한 천연 항산화 물질을 활용한 모발 화장품 개발이 필요한 시점이다. 이에 매우 탁월한 생리활성 성분이 이미 입증된 은행잎(Ginkgo biloba L.) 추출물을 도포 한 모발에 대한 최대 하중, 최대 인장강도, 최대 신장률, 파단 하중, 파단강도, 파단 신장률, 구간에 따른 Max. modulus와 Tangential modulus 값 등 다양한 융합적인 물성(物性)실험을 하였다. 연구 결과 은행잎 추출물을 도포 한 모발은 대조군에 비하여 인장강도를 포함한 고유 물성값이 전반적으로 증가하였다. 본 연구를 통해 은행잎이 기존 출시된 건강보조식품이나 의약품뿐만 아니라 퍼머넌트 웨이브 제재와 같은 모발 화장품의 유용한 재료로서의 가능성에 대해 연구를 하고자 하며, 다양한 모발 화장품의 후속개발을 위한 유용한 연구자료로 제공되기를 기대한다.

• 한국지역사회생활과학회지
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• 제18권3호
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• pp.391-398
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• 2007

Ginkgo leaves, which have been known as medical materials, were selected as new natural dyes in this study, in which it was examined whether they have the function of UV-blocker or not. Cotton, linen & Hanji(Korean traditional paper) were dyed with Ginkgo. Al, Cr, Cu, Fe and Sn were selected as mordants. UV-blocking rate of dyed samples was measured after dyeing and mordant treatment. The results of this study are as follows. First, most surface color of dyed samples was yellow except that Hanji mordanted with Cu was yellowish red. Second, dyeing repetition had positive correlations with K/S values of cotton ($r=.\;758^{**}$), linen ($r=.\;500^*$) and Hanji ($r=.\;819^{**}$), because K/S values were increased according to dyeing repetition. Third, solar UV-blocking rates had positive correlations with dyeing repetitions (p<0.01), because UV-blocking rates had increased according to dyeing repetition. Fourth, UV-blocking ability of Hanji was highest among samples, as UV-blocking rates of cotton and linen dyed after 3 dyeing repetition were up to 93%, Hanji's values were up to 98%. Therefore, it was proven that Ginkgo extracts used in this experiment as dyestuff have excellent dyeing ability and high UV-blocking ability. It is hoped that this work will lead to further research to confirm the physiological effects when human wear the clothes made from Hanji.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.244-250
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• 1993

Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

• 한국산림과학회지
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• 제82권4호
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• pp.337-346
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• 1993

본(本) 연구(硏究)는 상수리나무(Quercus acutissima)와 은행나무(Ginkgo biloba) 유묘(幼苗)를 대상으로, 12주간 pH 4.0 또는 pH 2.5의 인공산성우(人工酸性雨)(Simulated acid rain ; SAR)에 노출(露出)시켜 기공저항성(氣孔抵抗性)과 엽(葉)의 Wettability 및 해부학적(解剖學的) 변화(變化)를 파악(把握)하고자 실행(實行)하였다. 대조구로는 pH 6.5의 증류수를 살포(撒布)하였다. 상수리나무와 은행나무의 기공저항성(氣孔抵抗性)은 두 수종(樹種) 모두 SAR처리에 의하여 높아졌으며, 색소(色素)(Water blue solution)의 부착정도 측정(測定)에 의한 상수리나무 엽(葉)의 Wettability는 SAR처리에 의하여 증가(增加)하였고, Contact angle측정(測定)에 의한 은행나무 엽(葉)의 Wettability 역시 SAR처리에 의하여 증가(增加)하였다. SAR을 처리(處理)한 상수리나무 엽조직(葉組織)에서 표피세포, 책상조직 및 해면상조직의 부분적(部分的)인 피해(被害)가 관찰되었고, 모용(毛茸)(Trichome)의 현저한 감소(減少)와 Wax층(層)의 침식이 SEM을 통해 관찰(觀察)되었다. 은행나무의 엽조직(葉組織)은 pH 4.0처리에서는 피해(被害)를 관찰(觀察)할 수 없었으나, pH 2.5처리에서는 표피세포 및 통도조직의 붕괴가 관찰(觀察)되었고, SEM에 의한 엽(葉) 표면조직(表面組織)의 관찰(觀察)에서는 현저한 변화(變化)를 찾아볼 수 없었다.

• 농약과학회지
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• 제18권3호
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• pp.210-219
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• 2014

• Asian-Australasian Journal of Animal Sciences
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• 제32권7호
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• pp.966-976
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• 2019

Objective: The methane mitigating potential of various plant-based polyphenol sources is known, but effects of combinations have rarely been tested. The aim of the present study was to determine whether binary and 3-way combinations of such phenol sources affect ruminal fermentation less, similar or more intensively than separate applications. Methods: The extracts used were from Acacia mearnsii bark (acacia), Vitis vinifera (grape) seed, Camellia sinensis leaves (green tea), Uncaria gambir leaves (gambier), Vaccinium macrocarpon berries (cranberry), Fagopyrum esculentum seed (buckwheat), and Ginkgo biloba leaves (ginkgo). All extracts were tested using the Hohenheim gas test. This was done alone at 5% of dry matter (DM). Acacia was also combined with all other single extracts at 5% of DM each, and with two other phenol sources (all possible combinations) at 2.5%+2.5% of DM. Results: Methane formation was reduced by 7% to 9% by acacia, grape seed and green tea and, in addition, by most extract combinations with acacia. Grape seed and green tea alone and in combination with acacia also reduced methane proportion of total gas to the same degree. The extracts of buckwheat and gingko were poor in phenols and promoted ruminal fermentation. All treatments except green tea alone lowered ammonia concentration by up to 23%, and the binary combinations were more effective as acacia alone. With three extracts, linear effects were found with total gas and methane formation, while with ammonia and other traits linear effects were rare. Conclusion: The study identified methane and ammonia mitigating potential of various phenolic plant extracts and showed a number of additive and some non-linear effects of combinations of extracts. Further studies, especially in live animals, should concentrate on combinations of extracts from grape seed, green tea leaves Land acacia bark and determine the ideal dosages of such combinations for the purpose of methane mitigation.

• 한국응용곤충학회지
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• 제36권3호
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• pp.243-248
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• 1997

본 연구는 서울 및 인천 등지에서 도심지의 주요 가로수 중 하나인 은행나무의 잎을 식해하여 극심한 피해를 야기시켰던 검정주머니나방의 피해분포상황 및 생할경과습성을 조사키위해 지난 1994년부터 1996년까지 수행하였다. 검정주머니나방은 연 1회 발생하였으며 3령유충태로 월동한 후 5월중순~6월초순, 8월중순~9월말에 걸쳐 은행나무를 주로 가해하였다. 기주범위는 은행나무외에 벚나무, 조팝나무, 느티나무로 조사되었다. 유충은 7령 경과 후, 6월중순~7월초순에 용화하였으며 7월 초순부터 성충으로 우화하였다.