• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.478-486
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• 1995

Periodontal therapy for treatment of periodontitis involves the elimination of bacterial plaque and elimination of the anatomic defects by regenerative procedure. The purpose of this study was to evaluate on the biological effect of magnolia and Ginkgo biloba extract to the antimicrobial, antiinflammatory and cellular activity. Antimicrobial assay was performed with the diffusion method of the extract by measuring of growth inhibitory zone of B. cereus from blood agar plate. Effect of the extract to cellular activity of gingival fibroblast were examined using MTT method and measured the result with optical density on 570nm by ELISA reader. Inhibitory effects of $PGE_2$ production from gingival fibroblast was performed with the addition of $IL-l{\beta}$ and the extract to the well and examined to the product of $PGE_2$ from cell by ELISA reader. In vivo anti-inflammatory effect was performed with injection examined with clinically and histologically for their extent of mecrosis and inflammation. Antimicrobial activity of Magnolia extract showed significantly higher activity than that of control. However, GBE did not showed significant activity to compare with control, and mixture of Magnolia and GBE extract showed significantly higher activity than that of control. The effect of cellular activity to gingival fibroblast showed no significant differences of between control and Magnolia extract. However, GBE showed significantly higher rate of cellular activity to compare with control and even to PDGF-BB, and also showed same degree of cellular activity even though mixed with Magnolia extract. The inhibitory effect of $PGE_2$ production showed significantly reduction of $PGE_2$ production to compare with control, but its inhibitory effect was not much strong to compare with Indomethacin. In vivo, antiinflammatory effect of Magnolia extract to P. gingivalis injection of Hamster buccal check showed significantly reduction of inflammatory cell infiltration and tissue necrosis, but GBE showed no effect on the inhibition of inflammatory process. These results suggested that Magnolia and GBE extract possessed different kind of biological activity and also can be compensated on their activity with each other for elimination of bacterial plaque and anatonical defect.

• 한국산림과학회지
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• 제8권1호
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• pp.140-148
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• 1968

우리나라 전체(全體)에 분포(分布) 식재(植栽)되고 있는 은행나무 종자(種字)에 X-선(線)을 조사(照射)하여 발아율(發芽率), 발아장해(發芽障害), 지엽(枝葉)의 형태적변이(形態的變異) 및 초기(初期) 생육(生育)을 조사(調査)한데 대해서 몇가지 결과를 요약(要約)하면 다음과 같다. 1. 대조구(對照區)에 비(比)해서 저선량구(低線量區)(100R)에서는 발아(發芽)가 약간 촉진(促進)되는 경향이 있으나 500R구(區)에서 부터는 현저히 저하(低下)되었으며 2. 발아(發芽)의 $LD_{50}$은 765R으로 추정(推定)된다. 3. 묘장(苗長)에 있어서 저선량구(低線量區)(100R)에서는 감소(減少)의 차이(差異)가 가벼웠으나 타처리구(他處理區)의 감소현상(減少現像)에 대해서는 유의성(有意性)이 인정(認定)되었다. 4. 선량증가(線量增加)에 따라 엽면지수(葉面指數) 거의 직선적(直線的)인 관계(關係)로서 증가(增加)하며 5. T/R율(率)은 반비례적(反比例的)으로 작아졌다. 6. 생체중(生體重)과 건물중(乾物重)에 있어서는 대조구(對照區)에 비(比)해서 100R구(區)에서는 약간 증가(增加)되기도 하나, 250R구(區)에서부터는 감소(減少)되어서 유의성(有意性)이 인정(認定)되었다. 7. 선량(線量)에 따라서 식물체(植物體) 각부분(各部分)에서 다양(多樣)한 형태적(形態的)인 변이(變異)를 볼 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.266-274
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• 2013

The effect of different phytogenic feed additives on reducing odorous compounds in swine was investigated using in vitro fermentation and analyzed their microbial communities. Soybean meal (1%) added with 0.1% different phytogenic feed additives (FA) were in vitro fermented using swine fecal slurries and anaerobically incubated for 12 and 24 h. The phytogenic FAs used were red ginseng barn powder (Panax ginseng C. A. Meyer, FA1), persimmon leaf powder (Diospyros virginiana L., FA2), ginkgo leaf powder (Ginkgo biloba L., FA3), and oregano lippia seed oil extract (Lippia graveolens Kunth, OL, FA4). Total gas production, pH, ammonianitrogen ($NH_3$-N), hydrogen sulfide ($H_2S$), nitrite-nitrogen ($NO_2{^-}$-N), nitrate-nitrogen ($NO_3{^-}$-N), sulfate (${SO_4}^{--}$), volatile fatty acids (VFA) and other metabolites concentration were determined. Microbial communities were also analyzed using 16S rRNA DGGE. Results showed that the pH values on all treatments increased as incubation time became longer except for FA4 where it decreased. Moreover, FA4 incubated for 12 and 24 h was not detected in $NH_3$-N and $H_2S$. Addition of FAs decreased (p<0.05) propionate production but increased (p<0.05) the total VFA production. Ten 16S rRNA DGGE bands were identified which ranged from 96 to 100% identity which were mostly isolated from the intestine. Similarity index showed three clearly different clusters: I (FA2 and FA3), II (Con and FA1), and III (FA4). Dominant bands which were identified closest to Eubacterium limosum (ATCC 8486T), Uncultured bacterium clone PF6641 and Streptococcus lutetiensis (CIP 106849T) were present only in the FA4 treatment group and were not found in other groups. FA4 had a different bacterial diversity compared to control and other treatments and thus explains having lowest odorous compounds. Addition of FA4 to an enriched protein feed source for growing swine may effectively reduce odorous compounds which are typically associated with swine production.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.102-102
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• 2019

Soybean curd residue (SCR), known as a major waste product of soybean processing, is the water-insoluble fraction which is removed by filtration during soymilk production. For these reasons, SCR was usually considered as food waste. SCR might have a good potential as a functional food material, value-added processing and utilization. SCR contains high-quality protein and consists of a good source of nutrients, including protein, oil, dietary fiber, minerals, along with un-specified monosaccharides and oligosaccharides. Also, SCR might be a potential source of low cost protein for human consumption. However, SCR could be a source of bacterial contamination when during food processing. This study was aimed to investigate antibacterial capacity of natural product through detecting relationship between SCR and microbial. We isolated five bacterial strains from SCR and elucidated antibacterial activity of nature medicines to extend storage capacity of food made with SCR. Thus, the extract which showed antibacterial effects in Corynebacterium calloonae and Raoultella amithinolytica is a combination of seven kinds of extracts: Glycyrrhiza uralensis, Cudrania tricuspidata, Salvia miltiorrhiza Bunge, blueberry, Acorus gramineus, Ginkgo biloba L., Camellia sinensis. This study suggested that antibacterial activities of natural medicines could be used for extension of storage capacity in SCR-contained food.

• 한국연초학회지
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• 제22권1호
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• pp.51-57
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• 2000

The feeding preference of cigarette beetles, Lasioderma serricorne F., was tested using various leaf litters. The number of the trapped L. serricome was 45.25$\pm$10.44 at flue-cured leaf tobacco, Nicotiana tabacum L., 23.50$\pm$6.0 at chinese juniper, Juniperus chinensis L., 1l.75$\pm$4.99 at oak, Qqercus acutissiuma C., and 1l.50$\pm$2.52 at rice-straw, (Oryza. sativa L.). The response of oviposition was 93.20$\pm$26.22 at flue-cured leaf tobacco, 53.60$\pm$11.82 at chinese juniper, 48.20$\pm$20.90 at oriental arborvitae, Thuja orientalis L., 31.80$\pm$18.10 at cherry-tree, Prunus serrulata var. spontanea M., and 29.40$\pm$13.7 at rice-straw. However, the oviposition was respectively low at gingko, Ginkgo biloba L.,(5.40$\pm$2.97), turf grass, Zoysia japonica S., (5.20$\pm$13.7), and oak (3.00$\pm$l.41). The augmentation was maximum at chinese juniper (27.33$\pm$19.44 of emerged adults) followed by Magnolia obovata (8.50$\pm$9.33). Fifty percent of the tested species leaf litters including cherry-tree did not show any augmentation. The adult activities after hibernation were primarily found in May and June at Kwangju and Suwon, and in April at Chungju. The field activity of L. serricome at Suwon was mostly lower than that at other places, except in August at Chungju. The first appearance of L. serricome was observed earlier at Chungju and Kwangju than at Suwon, and the frequency of insect appearance was high in July, August, and September. L. serricome could hibernate by feeding on many kinds of plant leaf litters and it's population could be maintained in the open field in Korea.

• 혜화의학회지
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• 제5권1호
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• pp.185-198
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• 1996

After evaluation of antithrombotic effect of 40 herbs on platelet aggregation induced by ADP(Adenosine diphosphate), these results were obtained as follows: 1. Crude drugs exerting over 30 % of in Chinemys reevesii (Gray)hibition on platelet aggregation induced by ADP were Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elata Bl., Thea sinensis, Chinemys reevesii (Gray), Cuscuta chinensis Lam., Cervus nippon Temminck., Biota orientalis (L.) Endl., Coriolus versicolor, Cinnamomum cassia Presl., Sophora flavescens Ait., Amomum villosum Lour., Carthamus tinctorius L., Rubus chingii Hu., Poria cocos (Schw.) Wolf., Laminana japonica Aresch., Ligustrum lucidum Ait., Angelica sineusis (Oliv.), Cyperus rotundas L., Ginkgo biloba L., Zingiber officinale Rosc., Prunus persica (L.) Batsch., Schizandra chinensis (Turcz.) Baill. and Plantago asiatica L.. 2. Of crude drugs having showed over 50% of inhibitory effect on platelet aggregation, at the concentration of $100{\mu}g/m{\ell}$, the inhibitory rates were 82.2% in Ganoderma japonicum (Fr.) Lloyd., 55% in Panax ginseng C. A. Mey., 50.8% in Gastrodia elata Bl., while at the concentration of $200{\mu}g/m{\ell}$, antithrombotic rates were 89.4% in Ganoderma japonicum (Fr.) Lloyd., 59.2% in Panax ginseng C. A. Mey., 57.9% in Thea sinensis, 52.7% in Gastrodia elata Bl.. These results suggest that the study sholuld be necessary on antithrombotic effect of solvent fractions of Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elaha B1. and Thea sinensis and isolation of effective compound from above drugs.

• Current Research on Agriculture and Life Sciences
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• 제17권
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• pp.1-6
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• 1999

상추를 포함한 4종의 식물의 수용 및 에탄올 추출액이 상추종자의 발아와 생장에 미치는 영향을 조사한 결과는 다음과 같다. l. 은행, 상추, 돼지풀, 달맞이꽃의 수용 추출물은 상추종자의 발아 및 생장율을 현저하게 억제되는 경향을 보여 억제물질을 함유하고 있음을 알 수 있었다. 2. 추출용매별로는 에탄올 추출액이 수용추출액보다 억제효과가 큰 것으로 나타났다. 3. 공시식물의 종류에 관계없이 추출물의 농도 10%가 상추의 발아와 생장을 가장 크게 억제시켰고 희석되어질수록 그 효과는 낮아졌다.

• 한국산림과학회지
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• 제90권2호
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• pp.169-175
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• 2001

6개지역에 식재된 은행나무 182개체의 잎으로부터 DNA를 추출하여 I-SSR과 RAPD 표지자를 분석하였다. 15개 I-SSR primer를 사용하여 PCR을 수행해서 227개의 증폭산물 변이체를 확인하였고, 5개 RAPD primer를 사용하여 67개의 증폭산물 변이체를 확인했다. 6개 집단에 있어서 집단내의 유전적 다양성 정도는 유사한 것으로 나타났다(Shannon's Index, I-SSR : 0.35~0.40; 평균 0.38, RAPD : 0.31~0.38; 평균 0.35, 통합 : 0.35~0.40; 평균 0.37). 3개의 자료(I-SSR 표지자, RAPD 표지자 및 통합 자료)로부터 평가된 유전적 다양성 정도의 등급은 상호간에 일치하지 않았다. 유전적 다양성의 대부분이 집단내 개체간에 존재하는 것으로 나타났으며(I-SSR : 94.31%, RAPD : 93.62%, 통합 : 93.57%), 결과로써 집단간 분화정도는 상당히 낮게 나타났다. 수나무와 암나무 그룹간의 유전적 분화정도는 매우 낮았으며(I-SSR : 0.03, RAPD : 0.091, 통합 : 0.043), 수나무와 암나무들 동시에 채집한 3개 지역만을 대상을 분석했을 경우에는 약간의 변동이 있었다(I-SSR : 0.038, RAPD : 0.084, 통합 : 0.047). UPGMA법을 이용해서 분석한 집단간의 유전적 유연관계는 지리적 거리와 일치하지 않았는데, 이는 몇몇 지역들이 종자 공급원을 공유하고 있기 때문일 것으로 추정된다. I-SSR과 RAPD data를 성별로 재편성해서 유집분석을 수행한 결과, 모든 수나무 집단들과 암나무 집단들이 각각 별개의 성별 분지군을 형성하는 양상을 보였으나, 2개의 data를 통합해서 유집 분석을 수행했을 경우에는 성에 따른 명백한 분지군이 형성되지 않았다.

• 복식
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• 제56권3호
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• pp.73-80
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• 2006

This study is aimed to produce natural dyeing garments, which contain the sentiments and color sense of Koreans, by using various household supplies, with a view to increase the values added and utilization of natural dyeing. The dyes mainly used for this study are multi-colored ones which are durable after dyeing and can represent various colors. They include Lithodpermum officinale, Caesalpinia sappan, Rubia Cordifolia, Pellodendron Bak, Curcuma Longa L., fallen Ginkgo Biloba, Artemisia Princeps, and Quereus acutissima carr shell. Considering water pollution and other things, aluminum acetate and ferrous chloride were used as mordants. Cheap and practical materials such as cotton cloth and those with traditional patterns and elegance such as silk and ramie fabrics were used to produce baby goods, household supplies, clothes, and accessories. The produced supplies closely contact with our body, so they may have a great effect on human body. The household supplies produced include rug, bedclothes, room bedclothes, curtain, bedding set, cushion, and sunlight blind, in consideration of the functionality or medical actions of the goods which are closely related to the human skin. The infant supplies produced include longcloth for newborn baby, bedding set, gauze handkerchief, and doll. The clothes include night gown, shirt, bath gown, and Korean traditional clothes. The accessories include tablecloth, cup pad, bag, wrapping cloth, mouse and keyboard cushion, and scarf. Nowadays, consumers prefer environment-friendly naturally dyed garments and products. In order to control the naturally dyed garments and products which are traded in the domestic market, it is urgent to introduce such ecological marks as 'Toxproof' and 'Ecoproof.' Currently, many ordinary persons, who are not experts, have interest and participate in natural dyeing, but they simply produce naturally dyed garments, not artistic works or everyday supplies. The present author expects this study will contribute to the production of household goods by such persons. The author also hopes that they will develop and commercialize such designs as suit to modern tastes and senses based on the unique cultural assets of Koreans, so that Korean naturally dyed products may be recognized as reliable commodities equipped with international competitiveness.

• 한국환경복원기술학회지
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• 제7권2호
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• pp.36-45
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• 2004

This study was conducted to formulate management guidelines for monumental old trees in Korea through analysis of growing environments. A total of 20 old trees designated as natural monuments in Seoul, Incheon, and Gyeonggi provinces were surveyed for biological characteristics, surrounding environments, root collar conditions, tree health, and soil characteristics. Relationships among root collar conditions, tree health, and soil characteristics were analyzed by correlation. The old solitary trees designated as natural monuments included Pinus bungeana(4 trees), Juniperus chinensis(3 trees), Ginkgo biloba(3 trees), Poncirus trifoliata(2 trees), Actinidia arguta, Wisteria floribunda, Thuja orientalis, Quercus variabilis, Sophora japonica, Fraxinus rhynchophylla, Zelkova serrata, and Pinus densiflora. The tree height ranged from 3.56 to 67m, and root collar diameter ranged from 1.01 to 15.2m. The monumental old trees were growing on the various sites ranging from gardens, historical sites, open agricultural fields, mountain hills, to near the ocean beaches and streams. The coverage of bald land ranged from 50 to 100%, and depth of filled soil around the root collar ranged from 0 to 50cm. Tree health was expressed as the amount of branch dieback, cavity development, detachment of cambial tissue, infliction by diseases and insects. The branch dieback ranged from 5 to 20%, cavity development ranged from 10 to 100$cm^3$, detachment of cambial tissue ranged from 5 to 45%, and infliction by diseases and insects ranged from 5 to 20%. Soil pH ranged from 5.9 to 8.3, organic matter contents from 12 to 56%, phosphorus contents from 104 to 618ppm, while soil compaction ranged from 7 to 28mm. Results of correlation analysis showed that coverage of bald land was the most serious factor to deteriorate the cavity development and detachment of cambial tissue. In addition, chemical properties of soils seemed to be related to the health of the trees.