• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.56-58
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• 2007

This study was attempted to find appropriate surgery for gill cover curling disorder in arowana. Gill cover curling is a usual clinical findings in arowana, which often lead to death of the fish by dyspnea. Two different surgical methods were implemented in this study for gill cover by using cutting and incising fm-like fer two silver arowana. The surgery was identified to be successful since the fish healthily recovered. This experiment demonstrated that gill cover curling can be cured by using surgery.

• Journal of fish pathology
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• v.5 no.2
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• pp.93-118
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• 1992

The research was carried out to examine the chronic toxic effects of nitrite on the Japanese eel, Anguilla japonica by neans of histological observations. Young eel, 10.8g mean body weight. were exposed to 6 different concentrations of nitrite(1, 5, 10, 20, 30 and 40ppm) for 10 weeks. Each concentration was treated under 5 different levels of pH(5.5, 6.0, 6.5, 7.0 and 7.5) and each of these treatment was tested at 2 different temperature regimes($25^{\circ}C$ and $30^{\circ}C$). Proper concentration of nitrite was made by $NaNO_2$ and proper pH levels were made by the combinations of 0.1M $KH_2PO_4$ and 0.1M $NaHCO_3$. Histopathological test of gill tissues were made along with the test of the formation of thrombocystes and chloride cells on the gill filaments. At the lower pH levels, mucus secretion from the gill was incrased as the nitrite concentration increased. As the level of nitrite increased the number of chloride cells on the gill filament were decreased. Most of the remained chloride cells were observed only at the terminar part of the gill filament at 40ppm of nitrite. Degeneration of gill tissues were observed when nitrite levels were over 10ppm along with detachement and sweption of the epithelial cells of the gill lamellae. Shrunken gill lamellae and formation of thrombosis in the capillaries of gill lamellae were also observed. When temperature goes higher at the higher level of nitrite, necroses in the gill lamellae was increased. At the lower than 10ppm of nitrite, degeneration of gill lamellae was occured at the beginning of the test period but regenerated later. Negative effects of nitrite on the growth of young eel was started between 5~10ppm at the pH level of 7.0 and 7.5. Thrombosis formation were also started at this level. The safety concentration of nitrite at the pH levels of 7.0 and 7.5 on the small eel seems to be 1ppm. Thrombosis and gill lamella detachment and necrosis in the gill capillaries were not observed at this level. Chloride cells were appeared the whole part of the gill filament.

• The Korean Journal of Malacology
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• v.30 no.1
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• pp.51-56
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• 2014

Morphology and histochemistry of gill of the abalone, Haliotis discus hannai were described using light microscopy and scanning electron microscopy (SEM). The abalone has bipectinate gill of protobranch. The cilia and microvilli were well-developed on the free surface of the gill filaments. The epithelial layer of gill filament was simple and composed columnar epithelia and mucous cells. Result of AB-PAS (pH 2.5 and 1.0) and AF-AB (pH 2.5) reaction showed that mucous cells contained mainly acidic carboxylated mucosubstances.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.1
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• pp.7-10
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• 1975

The catches of mackerel by monofilament and multifilament net were compared by means of $x^2$ and t-test method. In order to improve the netting twine of drift nets, 6 shoots 5 different mesh and nylon-monofilament netting twines $(B\times2,\;C\;D,\;F,)$ and 2 shoe nylon-multifilament $(A\times2)$ which are using near the Jeju Island in contemporary days were used for the experiment. These gill no were connected in order of A, ,B, C, A, B, E, F and operated fly fishing boat Taeann Ho (7T) near sea of Jeju Ialand from May 1974 to August 1974. The results obtained are as follows: 1. B type nylon-monofilament gill nets were superior to nylon-multifilament gill nets in catch according to the result of $X^2$ test and t-test, and the catch ratio was $M_A:\;M_B=1:1.8$. 2. 75mm mesh size C, D nylon-monofilament gill nets were superior to 85mm mesh size nylonmonofilament gill nets, and their catch ratio were E, F: C, D=1:2.8. 3. The catch ratio C, D and E, F type nets were compared by means of t-teat, however could not recognized their relationship.

• Journal of fish pathology
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• v.1 no.2
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• pp.87-94
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• 1988

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.38 no.2
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• pp.91-100
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• 2002

The field experiments were carried out to examine for the mesh selectivity in trammel net and optimum mesh size, during Aug. 10~13, Non 9~13 2000 in Jumunjin, Kangwon-Do and during Jun. 1~27 2001 in Suyong-Bay, Busan. The experiments in Jumunjin area were conducted by using trammel nets with seven different mesh sizes (60, 75, 90, 105, 135, 150, 180mm) and those in Suyong-Bay were done by using trammel nets and gill nets with three different mesh sizes (60, 75, 90mm) in order to compare two types of nets of same structure. The mesh selectivity analysis was done mainly with flat fish(Eopsetta grigorjewi) that was occupied 60% out of total catches. And the mesh selectivity was written by extension of Kitahara's method included master curve with function type. The results obtained are summarized as follows 1. Total number of catch by, trammel net was 2,331 and that of gill net was 1,433, and flat fishes occupied 59.8 ~60% in total catches. 2. The value of optimum l/m by, trammel net was higher about 0.1 than that of gill net according as trammel net was 2.56, and gill net was 2.47. 3. The 50% selection range by, trammel net was wider about 1.5 time than that of gill net according as trammel net was 1.88~3.36(1.48) and gill net was 2.00~3.02(1.02). 4. The optimum mesh size(OMS) was estimated that trammel net was about 141mm and gill net was about 132mm on first maturity length(FML) 265mm of flat fish. 5. On the optimum mesh size based on the first maturity length of flat fish, The 50% range by, trammel net was wider about 76mm than that of gill net according as trammel net was 265 mm~474mm and gill net was 265mm~398mm.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.518-526
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• 2013

Gut-derived lipopolysaccharides (LPS) are critical to the development and progression of chronic low-grade inflammation and metabolic diseases. In this study, the effects of probiotics Lactobacillus and Bifidobacterium on gut-derived lipopolysaccharide and inflammatory cytokine concentrations were evaluated using a human colonic microbiota model. Lactobacillus reuteri, L. rhamnosus, L. plantarum, Bifidobacterium animalis, B. bifidum, B. longum, and B. longum subsp. infantis were identified from the literature for their anti-inflammatory potential. Each bacterial culture was administered daily to a human colonic microbiota model during 14 days. Colonic lipopolysaccharides, and Gram-positive and negative bacteria were quantified. RAW 264.7 macrophage cells were stimulated with supernatant from the human colonic microbiota model. Concentrations of TNF-${\alpha}$, IL-$1{\beta}$, and IL-4 cytokines were measured. Lipopolysaccharide concentrations were significantly reduced with the administration of B. bifidum ($-46.45{\pm}5.65%$), L. rhamnosus ($-30.40{\pm}5.08%$), B. longum ($-42.50{\pm}1.28%$), and B. longum subsp. infantis ($-68.85{\pm}5.32%$) (p < 0.05). Cell counts of Gram-negative and positive bacteria were distinctly affected by the probiotic administered. There was a probiotic strain-specific effect on immunomodulatory responses of RAW 264.7 macrophage cells. B. longum subsp. infantis demonstrated higher capacities to reduce TNF-${\alpha}$ concentrations ($-69.41{\pm}2.78%$; p < 0.05) and to increase IL-4 concentrations ($+16.50{\pm}0.59%$; p < 0.05). Colonic lipopolysaccharides were significantly correlated with TNF-${\alpha}$ and IL-$1{\beta}$ concentrations (p < 0.05). These findings suggest that specific probiotic bacteria, such as B. longum subsp. infantis, might decrease colonic lipopolysaccharide concentrations, which might reduce the proinflammatory tone. This study has noteworthy applications in the field of biotherapeutics for the prevention and/or treatment of inflammatory and metabolic diseases.

• Ocean and Polar Research
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• v.39 no.3
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• pp.221-232
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• 2017

The purpose of this study is to estimate the extent of fisheries resource rebuilding and other economic effects on coastal gill-net fishery as a result of the Korean vessel buy-back program using with-without analysis based on methods estimating sustainable yields for all species caught by coastal gill-net fishery. Based on the results of with-without analysis, maximum sustainable yields (MSY) of all species caught by coastal gill-net fishery have been increased by the Korean vessel buy-back program. In addition, profits per vessel of maximum economic yield (MEY) of the species have been improved by the program. Further, yields and a producer surplus per vessel under an equilibrium of open access (OA) have increased because of the program. In detail, first of all, at the MSY level, the vessel buy-back program has led to about 21% fisheries resource recovery, and at the MEY level, it has led to about a 19% resource recovery. Secondly, at the MEY level and the OA level, the producer surplus per vessel has been increased by about 24% and 22% respectively by the vessel buy-back program.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.2
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• pp.137-145
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• 2016

This study screened the biological activity of an acidified gill extract of the Manila clam Ruditapes philippinarum including antimicrobial, hemolytic, membrane permeabilization, and DNA-binding activity, and purified the antimicrobial material. The acidified gill extract showed potent antimicrobial activity against Bacillus subtilis and Escherichia coli without significant hemolytic activity, but showed no membrane permeabilization or DNA-binding ability. An antimicrobial material was purified from the acidified gill extract using C₁₈ reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). Treatment of the purified material with trypsin completely abolished all of the antibacterial activity against Bacillus subtilis, suggesting that the purified material is a proteinaceous antibiotic. The molecular weight of the purified material was 2571.9 Da, but no primary structural information was obtained due to N-terminal blocking. A future study should confirm the primary structure. Our results suggest that the Manila clam gill contains proteinaceous antibiotics that have a role in first-line defense. This information could be used to better understand the Manila clam innate immune system.

• Korean Journal of Ecology and Environment
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• v.53 no.2
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• pp.156-164
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• 2020

The accurate estimation of fish assemblages is highly dependent on the sampling gear used for sampling. We used data from 15 sampling sites along the Nakdong River, which is a large river in South Korea, to identify differences in assemblages and sizes of freshwater fishes collected with either cast nets or gill nets, the two most commonly used sampling gear in South Korea. The two gears differed in the fish assemblages they captured, with more species caught by gill nets. Further, due to its tighter mesh size, the cast net caught significantly smaller fishes than the gill nets(independent t-test, p<0.05). We found the cast net to be appropriate for species that inhabit shallow (less than 2 m) and open water, but inappropriate for deep water, habitats with plant beds, and nocturnal species. Thus, cast net sampling is not efficient in a large river environment, and a combination of sampling methods is more suitable for understanding fish assemblages in such habitats. In general, appropriate selection of fishing methods to specific habitats is necessary to improve data quality and minimize the misrepresentation of environmental conditions.