• Journal of Veterinary Clinics
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• v.33 no.4
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• pp.218-220
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• 2016

A 2-year old female African hedgehog (Atelerix albiventris) weighing 645 grams was presented with a well circumscribed mass in the subcutaneous of the left shoulder. It measured $2{\times}2cm$ in size and had a hard texture. There were no clinical signs. In a fine-needle aspiration (FNA) cytological evaluation, mast cells were identified with Wright-Giemsa stain. Mast cell tumor was confirmed and characterized by surgical sampling and histopathological evaluation. Four stains (hematoxylin-eosin, toluidine blue, periodic acid-Schiff, and Safranin O) were examined in order to find an applicable stain for a mast cell tumor histopathological evaluation in hedgehog. This case showed that periodic acid-Schiff and toluidine blue staining were the most useful for assessment of a mast cell tumor in a hedgehog.

• The Korean Journal of Cytopathology
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• v.5 no.1
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• pp.1-9
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• 1994

The cytological and ultrastructural findings of Pneumocystis carinii(PC) obtained from rats by bronchoalveolar lavage (BAL) are described. All developmental forms of the PC organisms were obtained in the lavage fluid. Papanicolaou stain revealed conglomeration of PC as a foamy cast. The cystic walls of PC were well identified on Gomori's methenamine silver stain. Trophozoites and intracystic bodies were stained by Giemsa and Diff-Quik techniques. Some PC organisms were seen within the alveolar macrophages. Ultrastructurally, the cysts were almost circular in shape, and were nearly devoid of surface tubular extensions. The wall of the cyst was composed of an unit membrane, an intermediate electron lucent layer and an external electron dense layer The cysts frequently contained intracystic bodies, so called sporozoites. Occasionally empty or collapsed cysts with no intracystic bodies, and precysts were found. Trophozoites were variable in size and shape with abundant tubular extensions along the single electron dense pellicle. BAL is a useful method for concentrating the various morphologic forms of PC organisms, and is a rapid diagnostic method for PC pneumonia.

• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.665-671
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• 1993

The rate of 67 neonatal calves's infection of Theileria sergenti was investigated in random samples on the farms located in Kyeongki, Chonbuk and Jeju districts of Korea. The criteria used in verifying infection with T sergenti included the detection of parasites by giemsa's stain and acridine orange stain in the blood smear slides. Further evidence of current or previous exposure to T sergenti was based on the demonstration of T sergenti-specific antibody and antigen by the western immunoblot and the indirect immunofluorescence antibody test in the peripheral blood of the calves. The prevalence rates were 35%, 50% and 100% in Kyeongki, Chonbuk and Jeju provinces respectively and the overall prevalence in all the farms was 43.2% by means of acridine orange stain. The parasites that were observed in the peripheral blood of calves was shown surely by the western immunoblot to the characteristic 34KD antigen among the proteins of T sergenti (Korean Isolate). And the antibody of the neonatal calves reacted at the very highest titer(1 : >2,520). These data highlight the significance of T sergenti in the neonatal calf disease in Korea.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.155-163
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• 1988

For the laboratory diagnosis of Sarcocystis infections especially in domesticated food animals, several antificial digestion techniques were applied for the musculature specimens and several staining techniques was applied for the bradyzoites of Sarcocystis species isolated. The digestion technique using trypsin(0.5%) and sodium chloride(0.85%) mixed solution was regarded as the most valuable for the detection of asexual stages of Sarcocystis in bovine musculature specimens. Optimal time for digestion was approximately one to four hours. The trypsion digestion technique with Giemsa's stain could be helpful for the detection of Sarcocystis prolferative forms and for the observation of the nucleus of the parasite. A systematic detection was also performed in an autopsy for a bovine carcass naturally infected with Sarcocystis species, and the asexual stages such as metrocytes and bradyzoites were observed in the specific organs, respectively.

• Journal of Veterinary Clinics
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• v.4 no.2
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• pp.505-514
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• 1987

Babesia gibsoni infection was diagnosed and treated in three hunting dogs which were hospitalized to the Veterinary Teaching Hospital, College of Veterinary Medicine, Seoul National University between April 4 and August 29, 1987. All three dogs revealed severe anemia, hemoglobinuria, splenomegaly and markedly decreased PCV, RBC count and hemoglobin. The anemia was regenerative, as characterized by increased numbers of nucleated erythrocytes, polychromasia, anisocytosis, reticulocytosis. B. gibsoni was identified by examination of blood smear stained with Giemsa stain. The forms of B. gibsoni identified in this report were pleomorphic such as singnet ring, oval, comma, dot and elongated forms. The maximal percentages of erythrocytes infected with one or more B. gibsoni organisms were 39%, 20% and 40%, respectively. The Tick, Haemophysalis longicornis was assumed to be the vector of babesiosis in these cases. Specific treatment consisted of diminazene aceturate and supportive treatment consisted of whole blood transfusion, lactated Ringer's solution, vitamin B complex and broad spectrum antibiotics. All three dogs had convalesced successfully after treatment.

• Journal of Yeungnam Medical Science
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• v.6 no.2
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• pp.23-28
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• 1989

After application of silver nitrate on every newborn, the incidence of gonococcal conjunctivitis was markedly decreased. But recently neonatal conjunctivitis due to chlamydia infection is increasing, so clinical observation was made on 26 newborn infants who showed eye discharge from June 1st to August 31st 1989. The results were as follows. 1. The incidence of chlamydia infection among neonatal Conjunctivitis was 34.6%. 2. The most common age at diagnosis was 6-15days of life and there was no sexual prepondrance. 3. There was no significant difference on clinical symptoms between chlamydia conjunctivitis and other Conjunctivitis. 4. Of 26 infants examined. 16 cases revealed no growth on routine bacterial culture. Of the organism cultured, P. aeruginosa was the most common agent(19.2%) and followed by S. aureus(11.5%) and S. epidermidis(7.6%). In one case of chlamydia I conjunctivitis, there was concurrent S. aureus infections. 5. On this study, Giemsa stain did not give significant diagnostic aid of chlamydia conjunctivitis.

• Korean Journal of Microbiology
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• v.22 no.4
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• pp.207-214
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• 1984

Improved methods for the isolation and purification of conidial protoplast were investigated and conidial proplast from auxotrophic mutants were fused. The reaction time for isolation of protoplasts from the swollen condiospores preincubated in liquid minimal medium supplimented with 2-deoxy-D-glucose was shorten by reaction with mixture of 2% driselase and 2% ${\beta}-glucuronidase$ (1:1). The conidial protoplast could be highly purified by using 5% Ficoll 400 as a centrifugation medium. Nucleus of the conidial protoplast was stained with Giemsa stain and the conidial protoplast had one nucleus. It was also confirmed that the conidial protoplast was true protoplast with no cell wall remnant at the outside of plasma menbrane. Fusion frequencies of the conidial protoplast from auxotrophic mutants ranged from $3.4{\times}10^{-1}\;to\;4.9{\times}10^{-1}$. These values were higher than those of mycelial protoplast by a factor of 5 to 28.

• Journal of Sericultural and Entomological Science
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• no.11
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• pp.63-68
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• 1970

The induction of polyhedroses in the silkworm, Bambyx mari L., was investigated treating the 5th instar larvae just after eodysis with high temperature (hot water bath at 40$^{\circ}C$ for 5 minutes or dry heat shock at 40$^{\circ}C$ for 30 minutes) and low temperature (5$^{\circ}C$ for 24 hours). The results obtained were as follows; 1. Comparing between the frequency of nuclear and cytoplasmic polyhedroses induced by cold and heat treatments (hot water bath at 40$^{\circ}C$ for 5 minutes), the induction ratio of the former is clearly less than that of the latter. But if the larvae tested with cold were left at room temperature (25$^{\circ}C$) for 30-120 minutes till the next hot water bath (40$^{\circ}C$) for 5 minutes and water bath (20$^{\circ}C$) for 5 minutes, treatments, the frequency of induced cytoplasmic polyhedrosis was more than that in the case of cold or hot water bath treatment alone. 2. The frequency of nuclear and cytoplasmic polyhedrosis induced by cold and successive heat (dry heat shock at 40$^{\circ}C$ for 30 minutes), left at room temperature (25$^{\circ}C$) ti11 the second treatment, the frequency of nuclear polyhedrosis was less than that of cytoplasmic polyhedrosis. 3. The reaction of nuclear polyhedra to stains also differs sharply from that of the cytoplasmic type. In a smear of nuclear polyhedra on a slide staining with Giemsa solution remains unstained against a stained back ground, in contrast to this, the cytoplasmic polyhedra take up stain readly.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.289-293
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• 1993

Following the death of more than 250 goats in one herd of 800 goats, imported from Australia, an epidemiological investigation was undertaken to determine the probable aetiology of this apprently mysterious disease. The syndrome was characterized by severe anemia(Hematocrit <20% ; normal range 24 to 48). All the affected animals were imported from Australia and all the motalities occurred during the period from September to November, 1992 Giemsa stain, acridine orange and indirect immunoflourescence tests were utilized in a survey involving 239 goats reared in Chonbuk Province. The positivity rates by acridine orange for anaplasmosis or piroplasmosis were 60.8% and 66.2% for imported and indigenous breeds respectively. It is tenatively concluded that the probable cause of death was anaplasmosis.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.644-646
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• 2007

This report deals with a 9-year-old neutered male Yorkshire terrier that had been suffered from vomiting, bloody diarrhea, anorexia. On exploratory laparotomy, pale-pink $10{\times}6cm$ duodenal mass was found and submitted to the Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University. Histologically, the duodenal mass consisted of compact sheet of poorly demarcated, highly infiltrative neoplasm. The neoplastic cells were round to polygonal and contained scant to moderate amounts of granular basophilic cytoplasm and eccentrically located irregularly round nuclei with stippled chromatins. The neoplastic cells were positive to lamda light chain immunohistochemically but were uniformly negative for toluidine blue stain and giemsa stains. Based on these results, this case was diagnosed as primary extramedullary plasma cell tumor of duodenum.