• Journal of Forest and Environmental Science
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• v.38 no.1
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• pp.38-47
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• 2022

Hymenodictyon orixensis (Roxb.) Mabberley (locally known as Bhutum in Bangladesh) is both an ecologically and economically valuable multipurpose tree species for afforestation and reforestation programs in Bangladesh. Seed germination and seedling development study of H. orixense were conducted to find out the response to different growing medium, e.g., polybag (15×10 cm (T₀) and 20×15 cm (T₁)), sand medium in propagator house (T₂), conventional nursery bed (T₃), and root trainer (T₄) in the Nursery. Consequently, germination behavior and seedling morphological parameters of H. orixense were assessed. The results revealed that the sand medium of the propagator house (T₂) provided the highest germination % (58.57±22.30) and the highest germination energy (11.43±2.43) followed by seedlings growing in 20×15 cm polybags (T1) containing forest topsoil and cow-dung at a ratio of 3:1. Except for germination energy, germination values, and germination capacity, other seed biology parameters, particularly imbibition, germination period, germination rate, and plant survival percent in T₁, T₂, T₃, and T₄ were significantly (p<0.05) different from T₀. Each phenotypic parameter of seedlings and dry matter of shoot and root significantly differed from control except root length (p<0.992). Based on this study, Polybags of 20×15 cm size are regarded as the best medium for quality seedling development of H. orixense. The nursery bed (T₃) had the lowest germination performance and developed more inferior quality seedlings. Thereby, 20×15 cm size of polybags with conventional soil and cow-dung media is recommended for maximum germination and to grow the quality seedlings of H. orixense in the Nursery.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.321-326
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• 2000

To compare germinability of primed seeds with that of water imbibed and nonprimed seeds at different temperatures, percent germination, number of days to attain 50% of the final germination percentage (T50) and mean number of days to germination (MDG) were evaluated at various temperatures ranged from $10^{\circ}C$ to $35^{\circ}C$. Primed carrot seeds in -0.50 MPa PEG 8000 showed higher percent germination at $10^{\circ}C$ or $35^{\circ}C$. Reductions in T50 and MDG values were observed at all germination temperatures when primed with the PEG. The PEG combined with 100 mM $K_3PO_4$ was not as effective as the PEG alone in improving the germinability of carrot seeds. Nonprimed lettuce seeds germinated only 2% at $30^{\circ}C$ or $35^{\circ}C$. However, priming with 50 mM $K_3PO_4$ increased germination to 40% at $30^{\circ}C$ and to 21% at $35^{\circ}C$. In contrast to the single treatment of 50mM $K_3PO_4$, priming lettuce seeds in a mixture of 50mM $K_3PO_4$ and 200 mM $KH_2PO_4$ resulted in a lower percentage of germination, but a significant greater reduction of T50 and MDG. Percent germination was increased when 200 mM $KH_2PO_4$-primed onion seeds and 100 mM $Ca(NO_3)_2$-primed Welsh onion seeds were germinated at $10^{\circ}C$ or $35^{\circ}C$. Combined treatments with $Ca(NO_3)_2$ for onion and with $KH_2PO_4$ for Welsh onion seeds did not improve percent germination, but they significantly reduced T50 and MDG.

• Journal of Korean Society of Forest Science
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• v.26 no.1
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• pp.23-30
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• 1975

To elucidate the dependability of seed variability determination by tetrazolium, 15 tree species were checked. For the comparison of potential germinability for these tree seeds, x-ray photography and routine germination test in a incubator were implemented. Though there were considerable differences of seed variability in percentage between germination test and tetrazolium test, the judgement by tetrazolium test and x-ray test gave similar results. These findings indicated that tetrazolium test is a reliable method if treated and inferred properly. Solution of 0.25 percent tetrazolium could give a complete colour reaction as well as 0.50 percent solution.

• Journal of Plant Biology
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• v.30 no.1
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• pp.11-20
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• 1987

Phenolic compounds, p-coumaric and p-hydroxybenzoic acids, known as inhibitors for development and growth of many pioneer species on early stage of succession were used for the test fo susceptibility in various herbs collected from abandoned agricultural fields in the vicinity of Amherst(U. S. A.). The percent inhibition was generally greater for p-coumaric acid than for p-hydroxybenzoic acid. Concentrations of 5$\times$10-5 and 5$\times$10-4M generally had no significant effects, but at 5$\times$10-3M was inhibitory to germination and growth of tested species. And the percent inhibition caused by the two phenolics was correlated (r=.843, p<.01). Also the indices of resistance for germination and elongation were significant (r=.695, p<.01) in this study. While Cirsium and Lepidium invading species of early stage of succession were sharply susceptible for toxic activity by phenolic acids.

• Journal of The Korean Society of Grassland and Forage Science
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• v.2 no.2
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• pp.16-20
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• 1981

To cultivate Dystaenia takesimana as a fodder crop, Its problems and solutions are summarized follows : 1. There are a wax layer and wings on the out side of seed coat of D. takesimana seeds, which seems to inhibit imbibition and cause delayed germination. 2. The root and shoot growth of D. takesimana are very poor in the early stage and weed control is quite troublesome. 3 The growth of D. takesimana is very poor the soil of low fertility and much depressed in summer. 4 . The percent germination could be increased by removing wings, soaking, high temperature treatment or by chemical scarification. 5. Compost is most effective for the growth of D. takesimana and lime dressing is desired in the soil of low pH. 6 . The seedling growth could be improved selecting and breeding the strains with better germination and root growth, at the germination and growth characteristics of D. takesimana are variable.

• Asian Journal of Turfgrass Science
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• v.9 no.3
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• pp.213-223
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• 1995

The study is aimed to understand effects of ethephon(lmg/1), ABA(0.lrng/l), BA(0.lmg/l). and NAA (0.lmg /1) soaking treatment on the seed germination of perennial ryegrass 'Dandy', creeping bentgrass 'Penlinks', tall fescue 'Arid' and kentucky bluegrass 'Nasaw'. These experiments are carried in vitro and their results are summarized as the followings ; In case of ethephon (lmg /1) soaking treatment of seed, the early germination of tall fescue 'Arid' and perennial ryegrass 'Dandy' was good without the soaking times. creeping bentgrass 'Penlinks' and kentucky bluegrass 'Nasaw' obtained the best results at 12 hr. soking treatment, and the longer the soaking time, the higher the germination percentage. The early germination percentage of tall fescue 'Arid' and perennial ryegrass 'Dandy' increased more highly at ABA(0. tmg /1) soaking treatment than at the control. The germination percent-age of creeping loentgrass 'Penlink' and kentucky bluegrass 'Nasaw' had respectively the best results at 6hr. and l2hr. soaking treatment. The early germination percentage of tall fescue 'Arid' and kentucky bluegrass 'Nasaw'increased more highly at BA (0.lmg /1) soaking treatment than at the control, and especially had the best results in 6hr. soaking treatment. perennial ryegrass 'Dandy' had the best results at l0min. soaking treatment, and the longer the soaking time, the lesser the germination percentage. The early germination of perennial ryegrass 'Dandy'. creeping bentgrass 'Penlinks' and tall fes- cue 'Arid' increased more highly at NAA (0.lmg /1) soaking treatment than at the control, and especially had the best results at l2hr. soaking treatment. However, the germination of Kentucky bluegrass 'Nasaw' seeds inhibited at NAA (0.lmg /1) soaking treatment. Key words: Ethephon, ABA, BA, NAA, Soaking treatment, Seed germination.

• Korean Journal of Plant Resources
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• v.29 no.2
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• pp.235-240
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• 2016

Factors affecting germination of seeds in the forms of various environment and chemical compounds. The present study was aim to produced effective seed propagation method of Astilbe koreana (Kom.) Nakai which had expected high value for the cut flower, ornamental and pharmaceutical material. Seed width and length ranged 0.62, 2.22 ㎜, respectively, and weight of thousand seeds was 40.5 ㎎. As result of imbibition test, showed moisture content of fresh seed (2.57%) increased rapidly by water-soaking treatment under 24 hours, recording to maximum value of 29.8%, and expansion of the seed coat was observed. Seed germination was the best at 15℃ and light conditions (40.8%) among temperature and light conditions treated. Percent germination of seeds was improved under the low (15, 20℃) than high temperature (25, 30℃). In addition, the seed was not germinated at dark condition regardless of temperature. Seeds of A. koreana thus seemed that it had low temperature germinability conditions. To improve germination rate, seeds were submerged in various concentrations of growth regulators such as GA₃ and kinetin, and minerals as KNO₃ and KCl. As a results, KNO₃ treatment, regardless of concentrations, promoted germination compared to control. Especially, percent of germination (77.8%), germination energy (96.1%), mean germination time (11.3 days) and T₅₀ (6.5 days) were effectively improved by treatment of KNO₃ 20 mM.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.267-271
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• 2007

This study was carried out to examine optimal hot water treatment time in Albizzia julibrissin seeds. Germination and moisture absorption characteristics among intact seeds, immersed seeds for 24 hours in distilled water and hot water treated seeds were surveyed. As result, treated seeds showed a highly significant difference with intact and immersed seeds (p<0.0l). Especially, treated seed for 2.0 minutes represented the highest percent of germination (PG). But mean germination time (MGT) did not have significant difference between non-treatment and treatments (p=0.502). Germination speed and germination performance index showed similar tendency with PG. In percent of moisture absorption (PMA) and moisture absorption rate constant (MARC) treatments had higher values than non-treatment whereas 2.0 minutes treatment was lower than non-treatment in initial moisture absorption rate (IMAR). In relation between germination properties and moisture absorption characteristics, all properties except MGT among germination properties had high correlations with PMA and MARC ($r=0.854{\sim}0.931$,p<0.01) whereas IMAR didn't have correlation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.303-303
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• 2022

The purpose of this study was to evaluate agronomic characteristics and identify the useful traits to utilize the wheat genetic resources for breeding programs by understanding the phenotypic variation among germplasm through multivariate analysis. In this study, a total of 394 wheat accessions were characterized for 15 agronomic traits using the National Agrobiodiversity Center (NAC) descriptor list, of which 31 accessions from 6 species and 363 unidentified accession (Triticum spp.) available at the NAC, Rural Development Administration (RDA), Korea. Growth characteristics such as leaf width, culm length, spike length, spikelet length, solid stemmed, days to heading, days to maturity, grain-filing period, and also seed characteristics such as width, height, area, perimeter, circle, solidity, and germination percent were studied. Among the 15 agronomic characteristics, the germination percent showed the smallest variation between resources (CV = 0.4%), and the spikelet length (CV = 66.5%) showed the highest variation. A strong positive correlation was found between seed traits such as seed height and seed area (r = 0.90), seed height and seed perimeter (r = 0.87) and seed length and width (r = 0.80). Principal component analysis (PCA) was conducted and the first five principal components comprised 76.7% of the total variance. Among the first five PCs, PCI accounted for 28.5% and PC2 for 20.0%. Wheat resources (394) were classified into four clusters based on cluster analysis, consisting of 215 resources(I), 117 resources(II), 48 resources(III), and 14 resources(IV). Among the clusters, the resources belonging to Cluster III showed the lowest seed width, height, area, and perimeter characteristics compared to other clusters. The wheat resources belonging to cluster IV had small seed width and low germination percent, but took longer to form heads and mature than resources in other clusters. These results will serve as the basis for further genetic diversity studies, and important agronomic characteristics will be used for improving wheat, including developing high-yielding and resistant varieties to biotic and abiotic stresses via breeding programs.

• Journal of Forest and Environmental Science
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• v.33 no.3
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• pp.226-232
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• 2017

Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.