• Journal of Forest and Environmental Science
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• v.36 no.3
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• pp.207-218
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• 2020

Ashwagandha is an important ancient medicinal crops, being affected with many diseases, among which leaf blight caused by Alternaria alternata has become the constraint resulting in huge yield losses. Continuous usage of chemical methods leads to environment, soil and water pollution. Whereas biological control of diseases is long lasting, inexpensive, eco-friendly and harmless to target organisms. In this context, it is aimed to evaluate five Trichoderma strains viz. Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 as bio-control efficacy against A. alternata and growth promoting effect in Ashwagandha. All the Trichoderma strains had varied antagonistic effects against the pathogen. In dual culture technique, the strain T. harzianum IMI-392433 showed maximum percentage inhibition of mycelial growth (54.89%) followed by T. harzianum IMI-392432 (53.83%), T. harzianum IMI-392434 (48.94%) and T. virens IMI-392430, (43.62%) against the pathogen, while the least inhibition percentage was observed with the T. pseudokoningii IMI-392431 (36.60%). The culture filtrate of the Trichoderma strain, T. harzianum IMI-392433 recorded highest inhibition on the mycelial growth (39.05%) and spore germination (80.77%) of pathogen and the lowest was recorded in T. pseudokoningii IMI-392431 (20.45 and 50%). Moreover, seeds treated with spore suspension of the strain T. harzianum IMI-392433 reduced the percentages of disease severity index significantly. The strain T. harzianum IMI-392433 also significantly increased seed germination %, seedling vigor and growth of Ashwagandha. The correlation matrix showed that root yield per plant of Ashwagandha had significant and positive correlation with plant height (r=0.726^⁎⁎), number of leaf (r=0.514^⁎⁎), number of primary branch (r=0.820^⁎⁎), number of secondary branch (r=0.829^⁎⁎), fresh plant weight (r=0.887^⁎⁎), plant dry weight (r=0.613^⁎⁎), root length (r=0.824^⁎⁎), root diameter (r=0.786^⁎⁎), root dry weight (r=0.739^⁎⁎) and fresh root weight (r=0.731^⁎⁎). The significant and negative correlation (r=-0.336^⁎⁎) was observed with the root yield and percentages of disease severity index. The study recognized that the T. harzianum IMI-392433 strain performed well in inhibiting the mycelial growth and reduced the percentages of disease severity index of pathogen as well as increased the plant growth in Ashwagandha.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.10-14
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• 2006

We tested the seed viability of Pinus rigida using accelerated aging to discover optimum times and temperatures far artificially accelerated aging. Seeds were artificially aged at different temperatures and during different tines. The seed viability was affected by the accelerated aging and by temperature with a decline in germination and seed vigor. The aging index of P. rigida seed was 0.31 at $35^{\circ}C$ and seed viability was nearly lost after aging treatment at $40^{\circ}C$ for 15 days. The optimum temperature of P. rigida far the aging test was decided to be approximately $37^{\circ}C$ on the basis of the aging index. Inorganic materials and conductivity of leaching solution from aging seeds increased with the increase of aging period. The accelerated aging test was considered to be a suitable method to evaluate the seed viability of tree species. Because seed characters are much different among tree species, however, more studies need to be done to discover the optimum conditions for aging by tree species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.2
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• pp.274-280
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• 2010

This study was to investigate the quality characteristics of noodles prepared with germinated brown rice and rice bran, and to determine the changes in GABA content and antioxidant activity of the noodles. These noodles were evaluated for cooking properties (cooking loss, swelling index, water holding capacity), total polyphenols, antioxidant activities (radical scavenging of ABTS and DPPH), and GABA contents. The addition of germinated rice bran in the range of 5, 10, and 15% showed a significant influence on cooking loss, although no effect on swelling index was observed. The raw and cooked noodles containing germinated brown rice and rice bran contained more GABA contents (2751.6~4176.7 and 5522.0~9617.8 nmol/20 g of fresh noodle, respectively) than those of non-germinated brown rice and rice bran. Noodles made with germinated brown rice and rice bran had similar total polyphenolics contents and antioxidant activities compared to noodles made with non-germinated brown rice and rice bran. Overall, the germinated brown rice could be used as a good ingredient to increase the nutritional value and antioxidant properties of wheat flour noodle without affecting the cooking properties.

• Applied Biological Chemistry
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• v.29 no.4
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• pp.339-345
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• 1986

Rheological characteristics of starch isolated from germinated corn were investigated and compared with those of nongerminated corn starch. Flow behavior of both starches were Bingham psudoplastic. Consistency index and yield stress of germinated starch solution were lower than those of nongerminated starch solution, while flow behavior index was nearly similar. Concentration dependency of both starch solutions were similar to each other but lower temperature dependency of germinated starch solution was observed. Time dependent characteristics of both starches showed thixotropic behavior, but due to germination, germinated starch showed higher structural decay under shear than nongerminated starch and its elastic properties was weaker.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.144-151
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• 2019

The establishment rate of foxtail millet seeds is greatly affected by soil environment conditions. To enhance germination viability and stable production of foxtail millet seeds, it is important to select seeds with a high density. Therefore, this study tested the selection of high-quality seeds using salt solution (specific gravity: 1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, and $1.040g\;L^{-1}$) and investigated their germination rates, percentages of emergence, and seeding quality. In this study, three varieties were tested: 'Hwanggeum', 'Samdachal', and 'Kyeongkwan1'. The thousand seed weight of all three varieties increased proportionally with specific gravity. The highest thousand seed weight was observed at the selection with $1.040g\;L^{-1}$ specific gravity, which also had the highest germination rate of 88.3%, 86.7%, and 90.6%, for 'Hwanggeum'; 'Samdachal'; and 'Kyeongkwan1', respectively. The results of seeding quality indicated that higher the specific gravity during selection, the higher were the values of plant growth (plant height, leaf length, stem diameter, root length, root weight, and stem weight). All the three varieties were found to be longer or heavier for seeds selected at the specific gravity of $1.040g\;L^{-1}$. The packing germination viability investigation found that higher the specific gravity for selection of seeds, the higher was the percentage of emergence (PE) and the emergence rate index (ERI). The PE was the highest for seeds selected at $1.040g\;L^{-1}$ specific gravity (85.3, 83.0, and 87.0%), and ERI was also as high as $2.82d^{-1}\;m^{-1}$ or more at $1.040g\;L^{-1}$. Selection of seeds with salt solution resulted in high germination viability of foxtail millet. Therefore, sowing seeds selected at $1.040g\;L^{-1}$ specific gravity is expected to help substantially in increased productivity.

• Journal of the Korean Society of International Agriculture
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• v.31 no.3
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• pp.278-285
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• 2019

The experiment was implemented to gain basic data for stable proso millet (Panicum miliaceum L.) production through germination viability and establishment uniformity based on seed refining under seed selection with specific gravity of proso millet seed. Seeds of three proso millet cultivars were carefully selected by adjusting NaCl into 9 levels (1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, 1.04 g·L^-1), and their standard germination rate, seeding quality and field emergence rate were investigated. Proso millets showed heavier thousand seed weight as specific gravity high. In standard germination, all of the 3 cultivars of selected seeding showed at least 91% germination rate. With respect to the 1.040 g·L^-1 selection by specific gravity, 'Hwanggeum' had 98.9%, 'Ibaekchal', 99.4%, and 'Hwangsilchal', 98.9%, recording the highest. Seeding quality was investigated and it was found that, compared with the non-selected seeds, selected seeds had longer plant heights and averaged 2.0, 2.2 and 1.9 cm, respectably. In terms of leaf length, only 'Hwanggeum' and 'Ibaekchal' showed distinctive differences. No significance was found in stem diameter and SPAD. The seeds selected using specific gravity all had longer or heavier average root length, root weight and stem weight. Compared with non-selected seeds, the specific gravity of 1.040 g·L^-1 had longer root lengths of 2.9, 3.0 and 2.9 cm. 1.040 g·L^-1 was also found to have the heaviest root weight, and stem weight. The field emergence viability investigation found significance solely in the mean emergence time (MET) by the cultivar. High significance was found in percent emergence (PE) and emergence rate index (ERI) in the specific gravity for the selection of seeds. All of the three cultivars showed higher PE and ERI as specific gravity selection high. The 1.040 g·L^-1 specific gravity selection had the highest PE of 90.7, 85.3 and 92.8%. For productivity increase based on improved proso millet germination rate and enhanced establishment uniformity, it seems favorable to employ 1.040 g·L^-1 specific gravity selection using salt solution.

• Journal of Korean Society of Forest Science
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• v.10 no.1
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• pp.29-39
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• 1970

Forest fires often destory forests that have taken years to grow in a few minutes. Forest fire therefore, is an important problem in forest management and have caused heavy losses to the nations economy. In order to resolve this problem many investigations have been made in many countries. However, ecological studies on the forest after accidental fire have not yet been made in Korea. In order to conduct such a study, a burned area on Mt. Samak which is located at Dukduwon-ri, Seo-myon, Chunsung-gun, Kangwon-do, was chosen as experimental plot in 1967. The remaining seeds were collected from the burned area, and investigations on their germination rate and their productivity were made comparing to those of the seeds of undemaged area, and following results were obtained. 1. The number of seed collected from the control plots were 740 while it was 537 from the test plots (Table 3, 4). It was considered that this difference between burned and unburned area was mainly due to the fact that some of the seeds had been burnt by the fire, and the unfavorable environmental conditions in the burned area was also considered to be a reason. In the germination rate in the control plots showed 28.1% while it was 3.2% in the test plots. This difference was considered to be due to complete loss of viability of the seed by burning and high heat. 2. In the test plots, sixteen seeds of the Alnus japonica were collected and six of these seeds germinated (index number 100) which was the highest germination rate among the species of collected seeds. From these results, it was considered that a high temperature (above $150^{\circ}C$) caused reduction of the germination rate (Quadrat. 1.2). Seeds of Carex lanceolata var. Nana, were appeared much more in the higher plots than in the lower plots and it seemed to be due to the fact that the forest floor plants were much more abundant in the lower plots than in the higher plots which is covered with shrubbery. And some small seeds midght be able to avoid the effect of fire being burried in the soil or under the gravel. 3. With Pinus densiflora, 43 seeds were collected, and 11 of these germinated in the control plots. However in the test plots, 11 seeds were collected and no seed germinated. This shows that the Pinus densiflora was the weakest in resisting to heat among the observed species in this study. 4. Without exception the germination rate showed a higher index in the herbs than in the woody plants and it is believed that the herbs produced more seed than the wood plants because of the abundance of herbs colony.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.245-252
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• 2018

Yam bean (Pachyrhizus erosus) is a subtropical plant belonging to the Fabaceae family, and is a tuberous vegetable used as various food material with a crisp and juicy taste. This study was conducted to seek optimum sowing time of yam bean in Korea. For this, we surveyed germination properties by the different temperatures and compared the accumulation temperature (AT) and dry matter production (DMP) on growth stages of yam bean by the different sowing times. Two types of varieties cultivated mainly in Korea, Thailand local variety (TLV) and Cheongunmanma cultivar (CGMM), was used. The germination rate of yam bean was 86.0~94.0% at above $18^{\circ}C$, and germination days was longer at lower temperature. The times for flowering and tuber formation of CGMM were later than those of TLV, and the AT required for flowering, tuber formation and hypertrophy of CGMM were higher than those of TLV by $293^{\circ}C$, $280^{\circ}C$ and $108^{\circ}C$, respectively. Also, DMP of shoot and tuber in CGMM were greater than those in TLV. In sowing at April 25, tuber formation was slower than sowing after that time, and harvest index (HI) was relatively low due to delayed formation and hypertrophy of tuber. In sowing after June 9, DMP of shoot was relatively greater in early growth, but tuber was not sufficient to enlarge due to lack of growth days by cold and frost in late October. In sowing May 10 and 25, DMP of tuber and HI were the highest, because the change of day length and temperature gave an advantage to vegetable growth and tuber development. All above suggest that it was suitable to sow seeds on May for increment of tuber productivity in Korea.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.2
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• pp.101-107
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• 2001

The experiment was conducted to investigate the agricultural utility of macro-porous calcium-silicate rnineral(CellCaSi) as topsoil mixture, as well as to estimate as a soil conditioner. The bulk density of CellCaSi which is consisted of various particle sizes ranging from 1mm to 3.35mm was about $0.42g/cm^3$, and its maximum porosity was approximately 81.4%. We also investigate gerrnination rates for Cabbage and Lettuce to obtain the suitable mixing ratios of CellCaSi with topsoil. Among 4 different mixing ratios, the germination rates of ropsoil mixed with 10% of CellCaSi were 94.1% and 64.6% for Cabbage and Lettuce, respectively resulted in the mosr suitable for germination. The growth rates for Cabbage and Lettuce showed thar 10% and 20% of CellCaSi treatments signification influenced the fresh weight. To observe the adsorption capacity of CellCaSi, CellCaSi was treated with a chemical fertlizer(N:P:K=18:18:18). lncreasing the contents of N, P and K, the amounts of adsorption by CellCaSi for these element also increased. The most suitable types nutrient resources for growth condition of Cabbage, and Lettuce were Fer-1 and Fer-0.5.

• Journal of Animal Environmental Science
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• v.11 no.2
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• pp.135-146
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• 2005

This study was carried out to investigate the effect of Synechocystis sp. KACC 91007 when added to a pig slurry or pig liquid fertilizer (PLF) on germination index (GI) of Chinese cabbage. The preliminary experiment involved the screening of inoculant levels which were; 0.05, 0.1, 0.2, and $0.3\%$, respectively. The $0.05\%$ level of inoculant was selected based on low phytotoxicity and high GI.. The PLF underwent a 107 day aerobic and anoxic processing conditions. The T-N, T-P, $NH_4$, and $NO_3-N$ concentrations of the untreated pig slurry were; 2,873, 753, 1,441.6, and 16.48 ppm, respectively. Using aerobic processing treatment, the fertilizer value of the PLF was 3,672, 164, 183.87, and 21.97 ppm, respectively. In contrast, the fertilizer value of the PLF processed under anoxic condition was reduced to 1,261, 68, 161, and 16.87 ppm. The GI value of the untreated PLF under aerobic and anoxic processing condition was 83 and $40.4^{*}\%$, respectively. With the addition of the $0.05\%$ microbial inoculant, the GI improved by more than 40 and $50\%$ respectively, when the PLF was processed under anoxic and aerobic conditions. The above findings proved that the aerobic processing of PLF for 107 days was better than anoxic and yielded higher T-N which is a macro-nutrient fertilizer material. Consequently, the addition of $0.05\%$ microbial inoculant resulted to a higher GI of the Chinese cabbage specifically under aerobic processing condition.