• Title/Summary/Keyword: germination experiment

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Effect of Sowing Time on Germination and Early Seedling Growth of Quercus floribunda Lindl.

  • Karki, Himani;Bargali, Kiran;Bargali, SS
    • Journal of Forest and Environmental Science
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    • v.34 no.3
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    • pp.199-208
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    • 2018
  • Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.

Pre-Sowing Seed Treatment: Effect of Deep Sea Water Priming on Germination of Wild Vegetables; Gondalbi (Cirsium setidens), Jandae (Adenophora triphylla var. Joponica Hara) and Deoduck (Codonopsis lanceolata Trautv.)

  • Yoon Byeong-Sung;Shrestha Surendra Lal;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.680-686
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    • 2006
  • This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at $24^{\circ}C$. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at $2^{\circ}C$ after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and $KNO_3$ priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3% $KNO_3$ and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water, $KNO_3$, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.

Seed Dormancy and Germination Characteristics of Prunus mandshurica (Maxim.) Koehne (개살구나무(Prunus mandshurica)의 종자휴면과 발아특성)

  • Seung Hyuk Yang;Young Hyun Kwon;Ye Eun Kim;Chung Ho Ko;Seung Youn Lee;Yong Ha Rhie
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.324-330
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    • 2023
  • This study was conducted to determine the dormancy types and optimal germination conditions of Prunus mandshurica seeds. The pericarp of P. mandshurica seeds was presumed to be the reason for their poor water absorption. After the pericarp was removed with a razor blade, germination was observed to be less than 20.0% at all temperatures except at 5℃, suggesting the presence of physiological dormancy. Germination gradually increased at 5℃ after the removal of seed coat, reaching a final germination rate of 86.7% at 14 weeks of incubation. Based on these results, P. mandshurica seeds have a physiological dormancy. When seeds with removed pericarp were subjected to cold stratification, the germination percentage (rate) in the control group was low even at 16 weeks; however, the germination percentages in the 4, 8, and 12-week cold stratification groups were notably high with 93.3, 73.3, and 100.0%, respectively. The control group in the GA3 treatment experiment with seed coats removed showed minimal germination, but at 10 weeks, the germination percentage rose to 98.3% when treated with GA3 at 100 mg/L. Thus, it is necessary to scarify or completely remove the pericarp of P. mandshurica seeds to promote germination. After pericarp removal, it is important to subject the seeds to cold stratification at 5℃ for at least 4 weeks or treat them with GA3 at 100 mg/L.

Effects of Simulated Sulfuric and Nitric Acid Rain on Growth and Seed Germination of Arabidopsis thaliana (인공 황산비 및 질산비가 애기장대의 생장과 종자발아에 미치는 영향)

  • 이석찬;박정안;박종범
    • Journal of Environmental Science International
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    • v.12 no.6
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    • pp.659-664
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    • 2003
  • The experiment was carried out to investigate the effects of sulfuric acid and nitric acid among the main components of simulated acid rain (SAR) on the growth of vegetative organs and seed germination of Arabidopsis thaliana. The Arabidopsis treated with SAR supplemented with sulfuric and nitric acids, respectively, showed 28% and 30% decrease of shoot and root growth compared to the control plants, and also many necrotic spots on leaf surfaces after SAR treatment were observed. The shoot and root length for plants grown with nitric acid rain was 14% and 17% lower, respectively, compared to the control, whereas those grown with sulfuric acid rain was 24% and 25% lower than control plants. When Arabidopsis seeds were sown in distilled water, germination rate was 100% after 7 days. However, 80% in SAR medium supplemented with sulfuric and nitric acids, 88% in sulfuric acid rain medium and 93% in nitric acid rain medium. The germination abilities of seeds harvested from SAR supplemented with sulfuric and nitric acids, sulfuric acid rain, and nitric acid rain were 73%, 73% and 94%, respectively. Consequently, sulfuric acids showed more inhibitory effects than nitric acids on the growth of vegetative organs as well as germination rates in Arabidopsis.

Effect of Alfalfa Plant Extracts on Germination and Early Seedling Growth of Forages

  • Chon, Sang-Uk;Choi, Seong-Kyu;Park, Sang-Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.310-315
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    • 2000
  • Alfalfa (Medicago sativa L.) plants have been reported to contain water-soluble substances that are autotoxic as well as allelopathic. Laboratory experiment through a petri-dish assay with imbibed seeds was conducted to evaluate both autotoxic and allelopathic effects of alfalfa leaf extracts on the germination and early seedling growth of alfalfa, red clover, crested wheatgrass, and Russian wildrye. Alfalfa seed germination was delayed dependent on extract concentration, with no difference in final germination at 72 hours. Root growth of alfalfa was stimulated up to 14% above control at very low concentrations of both leaf and stem extracts of alfalfa and was significantly reduced at extract concentration of more than 0.5g dry tissue/L (${gL}_{-1}$). Leaf extracts were generally more autotoxic for root growth than were stem extracts. Hypocotyl growth was not affected by all the concentrations of both leaf and stem extracts. Root length of legumes was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Hypocotyl growth of two legume plants and plant height of two grasses were not influenced by extracts. Seed germination and root growth of legumes were more inhibited by aqueous extracts of alfalfa leaf than were those of grasses. This result indicates autotoxic effect of alfalfa leaf extracts seems to be greater than allelopathic effect.

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Effect of Seed Maturity on Germination of Some Burley Tobacco Cultivars (종자의 성숙도가 Burley종 담배의 발아에 미치는 영향)

  • Ryu, J.H.;Bae, S.K.;Han, C.S.;Chu, H.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.258-261
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    • 1983
  • This experiment was conducted to evaluate the effect of early seed harvest on germination of tobacco(Nicotiana tabacum L.) seeds. Seeds of seven burley tobacco were harvested every two days from 8 to 30 days after pollination and tested for germination. The results are; 1. Seeds harvested 12days after pollination germinated but germination rates were low and varied across cultivars. These seeds will provide viable seeds adequate for breeding program. 2. Germination rates of seeds harvested 24-28 days after pollination were high and showed none significant differences among cultivars. 4. Over-ripen seeds showed low germination rates cused by after-harvest or temporary dormancy.

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Effects of Fertilizer and Sewage Sludge Treatments on Germination and Growth of Woody Plants in Metal Mine Tailings

  • Lee, Sul-Ki;Cho, Do-Soon
    • The Korean Journal of Ecology
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    • v.23 no.6
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    • pp.445-452
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    • 2000
  • The effects of sludge and fertilizer application on germination and seedling growth of woody plants on heavy metal mine tailings were evaluated by greenhouse experiment. Two different mine tailings (Lead-zinc mine tailings from Kwangmyong, Kyonggi-do and tungsten mine tailings from Sangdong. Kangwon-do). four fertilizer treatments (N +P +K: 20, 40, 60, and 80 kg/m$^3$), and four sewage sludge treatments (5.5, 11, 22.5, and 45 Mg/m$^3$) were used in the experiment. Tested plants were Pinus densiflora, Larix leptolepis, Amorpha fruticosa, and Alnus hirsuta. There were three replicates for each treatment. In addition, vermiculite was used instead of mine tailings to determine the effect of physical amendments. Fifty seeds of a species were sown in a pot (upper diameter 13.5 cm, depth 10 cm) and seedling emergence were recorded daily for 30 days. The highest germination rate was 53% for all treatments. Germination rate of Larix leptolepis was lowest among the four species studied. One month later after seeding, seedlings were thinned and only 5 seedling were left in each pot, and fertilizer and sewage sludge were applied once again. Growth of seedlings were determined for 10 weeks since then. Most plants grew very poorly or died within 5 weeks on lead/zinc mine tailings from Kwangmyong. The analysis of heavy metal contents by the total dissolution method showed that heavy metals generally increased in the order of tungsten mine tailings from Sangdong < sewage sludge from Puchon < lead/zinc mine tailings from Kwangmyong. Growth of woody plants was improved significantly by the fertilizer treatments on tungsten mine tailings. In contrast. survival and growth of woody plants were not affected significantly by the sewage sludge treatment on both tailings. This study shows that fertilizer applied to established seeded stands may provide some benefits in terms of increased ground cover in the field. It is suggested that reclamation should be proceeded by the study of the physico-chemical and biological characteristics of mine tailings.

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Allelopathic Effects of Medicinal Plants on Echinoclhoa crus-galli Beauv. (약용작물의 Allelopathy 효과에 관한 연구)

  • Kim, Sang-Yeol;Kim, Chil-Ryong;Park, Sung-Tae;Kim, Soon-Chul
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.407-412
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    • 1997
  • Allelopathic activity of methanol extract from medicinal plants was determined based on the inhibition of germination, shoot and root length of Echinochloa crus-galli. The response was depended on the source of extracts and concentrations. Of the 20 test plants tested, Cnidium officinale showed most inhibition according to germination, shoot and root length of E. crus-galli. Based on the plant parts, the root extract of C. officinale had more inhibitory effect on E. crus-galli than the stem+leaf extract. Phytotoxic effects of the root extract on E. crus-galli showed 75% inhibition of seed germination, 95% inhibition of shoot growth, and 100% inhibition of root growth at the concentration of 1g/petridish. Sequential partitioning of root extract with organic solvents with increasing polarity showed that the ethylacetate fraction had the greatest inhibitory effect on E. crus-galli. Germination was not inhibited at any rate of ethylacetate fraction but shoot and root growth, were significantly inhibited even at 2mg/petridish. This indicates that the most toxic phytotoxin present in the root extract may be moderate polar compound.

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