• 한국환경농학회지
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• 제21권3호
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• pp.202-207
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• 2002

참박과 호박 채종종자의 발아율을 증진시킬 수 있는 종자처리 방법을 확립하고자 저선량 감마선을 1$\sim$20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 그 효과는 종자상태에 따라 다르게 나타났다. 참박 70$\sim$80% 발아율종자의 발아는 4$\sim$20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80$\sim$90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70$\sim$80%와 80$\sim$90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70$\sim$80% 발아율종자는 2 Gy 조사에서, 80$\sim$90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70$\sim$80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy 조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다.

• 한국연초학회지
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• 제25권1호
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• pp.47-52
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• 2003

The purposes of this study were to examine effect by Solid Matrix Priming(SMP) ; method far enhanced rapid and uniform seedling emergence in tobacco seeds. The results were as follows. No enhancement of germination rate was by SMP treatment. In KF114 during 9 days treatment and in KB108 during 7 days treatment, germinative energy was highest, and T50 and mean germination time were the shortest. Cells in embryo and hypocotyl were divided and extended during SMP treatment. The contents of fatty acid were increased in SMP treatment. The changes of glucose content were little.

• 원예과학기술지
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• 제33권6호
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• pp.932-940
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• 2015

One of the main factors restricting production of triploid seedless watermelon is poor germination due to weak embryos, thick seed coats, and larger air spaces. This study was carried out to investigate the priming effects of different concentrations of chemicals, including hydrogen peroxide ($H_2O_2$), fusicoccin, and gibberellic acid (GA) on germination and seedling uniformity of triploid watermelon (Citrullus lanatus). Three commercial triploid cultivars, Seedless Plus, Sinus, and Sizero, were pretreated with water and different levels of $H_2O_2$ (2 and 4%), fusicoccin (FC: 1, 5, and $10{\mu}M$), and GA (1, 5, and $10{\mu}M$). The present findings helped to find optimal priming conditions for improving germination of triploid watermelon. Treatment with $5{\mu}M$ GA and hydropriming helped to break seed dormancy, enhancing the final germination percentages in all triploid cultivars and increasing the germination index in Sizero. These seed-priming treatments could be used on large scale for industrial applications. Moreover, hydropriming provides a simple, effective, and costless method to improve seed germination and seedling vigor of Sinus and Sizero varieties.

• 한국작물학회지
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• 제48권1호
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• pp.13-16
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• 2003

Seeds of burcucumber were treated with accelerated aging, cold-stratification, and light quality illuminated during desiccation to enhance their germination and seedling emergence. The germination was increased by aging and cold-stratification although the latter treatment showed greater effectiveness than the former one. In the combined treatment of aging 6 days at $45^{\circ}C$ and cold-stratification, the germination was promoted under longer period of cold-stratification to reach nearly 100% in 3 week cold-stratification on the ninth day from sowing. In the sequentially combined treatment of aging, cold-stratification, and light quality during 24 hour desiccation at $35^{\circ}C$, no-stratified seeds showed the highest rate in red light treatment but the lowest in far-red light. This implies that the phytochrome action run during the desiccation of imbibed seeds. The red light exposure during drying for the cold-stratified seeds after aging accelerated the germination even more than the dark treatment and germinated 100% on the next day of sowing. It is concluded that the sequential treatment of aging, cold-stratification, and red light illumination during desiccation can highly promote percentage and speed of burcucumber seed germination.

• 생명과학회지
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• 제16권5호
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• pp.711-715
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• 2006

복숭아는 다른 과수에 비하여 개화기가 빨라 저온으로 인한 화분발아 장해로 결실율이 저하되고 있다. 이러한 문제점을 해결하기 위하여 저온에서 복숭아의 화분 발아를 증진시킬 수 있는 방법을 강구하고자 화분발아 배지에 아미노산, polyamine, flavonoid 등을 첨가하여 일련을 실험을 수행하였다. 저온에서 배지내 첨가물질로써 asparagine, glutamine, spermine, putrescine, kaemferol이 유효하였으며, 이들의 적정농도는 asparagine과 glutamine는 10 mM, spermine 100 mM, putrescine 1000 uM, kaemferol은 1.0 uM 이였다. 저온에서 발아율이 높았던 처리는 $100\;mg{\cdot}L^{-1}\;H_3BO_3+10\;mM$ asparagine 혼용조합이었고, 다음이 $100\;mg{\cdot}L^{-1}\;H_3BO_3+10\;mM$ glutamine 혼용조합, $100\;mg{\cdot}L^{-1}\;H_3BO_3+200\;{\mu}M$ spermine 혼용조합 순이었다. 기본배지에 치상된 ‘장택백봉’의 화분 발아율은 25.4%, '수봉' 13.9% 불과하였으나, $100\;mg{\cdot}L^{-1}\;H_3BO_3+10\;mM$ asparagine 첨가된 배지는 두 품종 모두 대조구보다 화분 발아율이 $18{\sim}19%$ 증진되었다. 아미노산과 폴리아민 첨가에 의한 화분발아율 증진 효과는 '장택백봉' 보다는 '수봉'에서 더욱 현저하였다.

• 생명과학회지
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• 제18권8호
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• pp.1096-1105
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• 2008

Kentucky bluegrass와 Tall fescue에서는 발아력을 극대화시킬 수 있는 priming 최적 처리 조건은 100mM의 $K_3PO_4$ 용액으로 $25^{\circ}C$에서 1일간 처리였다. Priming 처리된 종자는 무처리에 비해 높은 발아율을 보였고, $T_{50}$과 MDG가 단축되어 신속한 발아를 유도하였다. 반면 NaOH나 KOH는 처리농도가 높아지고 처리기간이 길어질수록 발아력이 급격하게 저하되어 priming 화학제로는 적당하지 않았다. Creeping bentgrass 및 Bentgrass에서는 priming 처리에 관계없이 발아율이 10% 미만으로 낮았다. 그러나 200mM의 $KNO_3$ 로 3일간 priming 처리하면 발아율을 6%까지 향상시킬 수 있었으나, 현저한 수준은 아니었다. Kentucky bluegrass와 Tall fescue에서 priming 처리 기간은 1일 처리가 발아율 향상에 가장 좋았고, 처리 기간이 6일로 길어질수록 발아율이 저하되었고 발아 속도는 지연되었다. 또한 priming 화학제의 처리 농도는 100 mM 처리가 발아력 증진에 좋았으나, 200 mM과 300 mM로 처리농도가 높아지면 발아율이 감소하였다.

• 한국자원식물학회지
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• 제19권6호
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• pp.680-686
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• 2006

This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at $24^{\circ}C$. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at $2^{\circ}C$ after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and $KNO_3$ priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3% $KNO_3$ and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water, $KNO_3$, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.101-109
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• 2016

This is the fundamental research on restoring damaged vegetation areas in the vicinity of DMZ using local native plant species. This research is aimed at identifying effective plant growth regulators (PGR) for seed germination and seedling growth of mountain mulberry, and developing effective methods for managing its germination and growth. Mountain mulberry seeds were collected from the regions in the DMZ vicinity, and tests with seven treatments using four PGRs including $GA_3$, kinetin, ABA and NAA, were conducted. The germination rate was calculated in two different ways of both in a growth chamber and in a greenhouse after seven days observation, and the growth characters such as leaf width/length, seedling width/length and fresh/dry weight, have been surveyed in a greenhouse for three months. Although in the growth chamber the highest germination rate was shown at a group with the kinetin treatment, it was not significantly different to that of the control group. Groups with ABA or NAA presented relatively low germination rates. As for the greenhouse test, the germination rates of all groups ranged 20~30% without significant difference each other, the reason of which might be due to low absorption by the effect of a soil drench method used in this study. The entire growth characters with the treatments of $2.15mg{\cdot}L^{-1}$ of kinetin and $10mg{\cdot}L^{-1}$ of $GA_3$ were significantly different to the control. NAA treatment only showed better growth of seedling width compared to the control. Consequently, the most effective PGRs for the germination and growth of mountain mulberry near the DMZ was kinetin. Further research on examining the most effective concentration of them was needed.

• 한국약용작물학회지
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• 제11권2호
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• pp.135-142
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• 2003

본 연구는 마가목의 발아율 제고 및 육묘방법을 구명하기 위하여 연구한 결과를 요약하면 다음과 같다. 마가목 종자는 무처리에서 22.6%정도의 저조한 발아율을 보였으나, BA 200ppm을 20분간 처리에서는 78.9%정도로 발아율이 높았다. 산사나무 종자는 최고 11.1%의 발아율을 보였으나, 화학약품과 생장조절제 처리에 의한 발아율 증가는 없었다. 출현율은 상자 육묘와 포트육묘 재배 시 93-95%로 높았으며, 49공 포트육묘 재배 시 육묘기간 50일 묘에서 근장이 길고, 건조근중이 높아 근생육이 양호하게 나타났다. 상자육묘 재배 시 육묘기간이 40일 묘에서 초장, 건조엽중이 포트육묘 보다 높았으며. 결과적으로 마가목의 실생육묘시 포트육묘로 50일 재배 후 이식하는 것이 가장 양호 하였다.

• Journal of Applied Biological Chemistry
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• 제57권2호
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• pp.153-157
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• 2014

Plant-growth-promoting rhizobacteria can affect plant growth by various direct and indirect mechanisms. This study was conducted to determine the ability of some rhizobacterial strains to enhance the seed germination of Lactuca sativa (lettuce) and Raphanus sativus (radish). Seeds were inoculated using a spore suspension ($1{\times}10^7cfumL^{-1}$) and incubated in a growth chamber at $28^{\circ}C$ under dark conditions and 65% RH. Azotobacter chroococcum and LAP mix inoculation increased the plumule length of L. sativa by 1.3, 0.8, and 0.7 cm, respectively, in comparison to the uninoculated control. Pseudomonas putida showed an increase of only 0.6 cm in plumule length when compared to the control. Inoculation of A. chroococcum, P. putida, and LAP mix enhanced the seed germination rate of R. sativus, by 10, 5, and 30%, respectively, in comparison with the uninoculated seeds. The results demonstrated that the inoculation of seeds by select rhizobacterial strains showed remarkable enhancement to the radicle length of lettuce and radish seedlings.