• The Plant Pathology Journal
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• 제26권4호
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• pp.321-327
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• 2010

We performed diagnostic PCR assays and a phylogenetic analysis using partial sequences of TEF1 (translation elongation factor-1) to determine the trichothecene chemotypes and genetic diversity of F. graminearum isolates from maize and rice samples collected in 2009 in Korea. PCR using a species-specific primer set revealed a total of 324 isolates belonging to the putative F. graminearum species complex. PCR with trichothecene chemotypespecific primers revealed that the nivalenol (NIV) chemotype was predominant among the fungal isolates from rice (95%) in all provinces examined. In contrast, the predominant chemotype among the corn isolates varied according to region. The deoxynivalenol (DON) chemotype was found more frequently (66%) than the NIV chemotype in Gangwon Province, whereas the NIV chemotype (70%) was predominant in Chungbuk Province. Phylogenetic analysis showed that all DON isolates examined were clustered into lineage 7, while the NIV isolates resided within lineage 6 (F. asiaticum). Compared with previous studies, the lineage 6 isolates in rice have been predominantly maintained in southern provinces, while the dominance of lineage 7 in maize has been evident in Gangwon at a slightly reduced level.

• 한국안전학회지
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• 제32권6호
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• pp.81-88
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• 2017

This study deals with the optimal design of a hybrid control system composed of a combination of active control system and passive control system for effective seismic performance improvement of two adjacent structures. The proposed hybrid control system adopts a configuration of installing an active control device in one building and connecting two adjacent structures with a passive control device so that the one-side active control force can be bi-directionally applied to both buildings through the passive connecting devices. In order to derive the optimal performance of the proposed system, the design parameters of the passive and active control systems were searched using the genetic algorithm. Numerical simulations of 10-story and 8-story buildings have been performed to verify the effectiveness of the proposed technique. For the purpose of comparison, the conventional independent control system with two identical active control systems being installed separately for each structure was also optimally designed and its seismic response has been evaluated as well. From the comparative results of the two control systems, it is demonstrated that the proposed hybrid control system requires larger control force for its one-side active control device than the conventional independent control system does for each of both-side active devices, but quite less than the total control force required for both-side devices of the independent control system, while maintaining similar seismic performance. Therefore, the proposed system is more economical and reliable than the conventional independent control system with two identical active devices.

• Journal of Microbiology and Biotechnology
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• 제21권8호
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• pp.777-790
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• 2011

To elucidate the biodiversity of plant growth-promoting rhizobacteria (PGPR) in Korea, 7,638 bacteria isolated from the rhizosphere of plant species growing in many different regions were screened. A large number of PGPR were identified by testing the ability of each isolate to promote the growth of cucumber seedlings. After redundant rhizobacteria were removed via amplified rDNA restriction analysis, 90 strains were finally selected as PGPR. On the basis of 16S ribosomal RNA sequences, 68 Gram-positive (76%) and 22 Gram-negative (24%) isolates were assigned to 21 genera and 47 species. Of these genera, Bacillus (32 species) made up the largest complement, followed by Paenibacillus (19) and Pseudomonas (11). Phylogenetic analysis showed that most of the Grampositive PGPR fell into two categories: low- and high- G+C (Actinobacteria) strains. The Gram-negative PGPR were distributed in three categories: ${\alpha}$-proteobacteria, ${\beta}$- proteobacteria, and ${\gamma}$-proteobacteria. To our knowledge, this is the largest screening study designed to isolate diverse PGPR. The enlarged understanding of PGPR genetic diversity provided herein will expand the knowledge base regarding beneficial plant-microbe interactions. The outcome of this research may have a practical effect on crop production methodologies.

• 대한안전경영과학회지
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• 제22권1호
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• pp.45-50
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• 2020

The number of elevators in Korea has surpassed 700,000 units in 2019, which is the 8th in the world by number of installed units and 3rd in the world by new units. The word 'lift' is a representative word, and the category includes elevators, escalators, dumb waiters, and moving walks. Those who live in the city will experience using elevators once or twice a day, and these elevators are becoming an indispensable means of transportation when using high-rise apartments or subways. However, such a convenient elevator also has a lot of risks that threaten the safety of the user and actually cause many accidents every year. In particular, escalators (including moving walks), which account for as little as 5% of all elevators, account for 70% of all elevator accidents. According to Heinrich's chain of thought theory, accidents are caused by a combination of factors, which are divided into five stages: Stage 1: Genetic Factors and Social Environment, Stage 2: Individual Defects, Stage 3: Unsafe behavior and Unsafe conditions, Stage 4: Accident, Stage 5: Injury. Heinrich said that three of these five phases, unsafe behavior and unsafe conditions, require safety management and efforts to prevent accidents. In escalator accidents, the analysis of accident cases that have occurred so far will be related to unsafe behaviors and unsafe conditions, and the effective management of these causes of accidents will enable safer and more convenient use of escalators. This study analyzed accident cases of elevator users, focusing on escalator accidents over the last 10 years (2010 ~ 2019), and safety management to prevent safety accidents of elevator users by analyzing the behavior of actual users and questionnaires of experts in related fields. The method was studied.

• 한국컴퓨터정보학회논문지
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• 제4권2호
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• pp.95-104
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• 1999

자동차가 증가함에 따라 교통 안전화를 목적으로 한, 자동차 전반에 관한 연구들이 주요한 과제로 대두되고 있다. 무인화 주행을 위한 시각 시스템은 미리 알려져 있지 않은, 일반적인 도로에서 주행하기 위해서는 연속적으로 입력되는 도로정보로부터 도로정보의 모델을 생성하여 주행에 필요한 도로정보를 추출한다. 본 논문에서는 자동 항법 시스템 중에서 자동 조종에 필요한 도로정보 표지판의 추출 방법을 하프변환과 유전자 알고리즘을 이용하여 제안하고자 한다.

• 한국공작기계학회논문집
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• 제18권1호
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• pp.103-109
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• 2009

This paper presents structural design optimization of a micro milling machine for minimum weight and compliance using a genetic algorithm with dynamic penalty function. The optimization procedure consists of two design stages, which are the static and dynamic design optimization stages. The design problem, in this study, is to find out thickness of structural members which minimize the weight, the static compliance and the dynamic compliance of the micro milling machine under several constraints such as dimensional constraints, maximum compliance limit, and safety factor criterion. Optimization results showed a great reduction in the static and dynamic compliances at the spindle nose of the micro milling machine in spite of a little decrease in the machine weight.

• 식품과학과 산업
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• 제52권2호
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• pp.171-187
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• 2019

Novel food materials can be produced based on biotechnology such as genetic recombination, microbial fermentation, and enzymatic engineering by utilizing living organisms such as animal, plant, and microorganism or by applying the enzymes isolated from them. Especially, exploration and development of novel prebiotics and probiotics attracted great attention worldwide in the food industry, of which the research and industrial trends in food biotechnology field are promoting the production of next generation sweeteners and proliferation of beneficial bacteria in gastrointestinal tract. Development and commercialization of novel food materials by domestic bioprocessing technology have been sluggish due to the GMO/LMO food safety issues. Meanwhile, the US and EU do not perceive badly about gene manipulation technology, and the research is most active in the fields of crops and GMMs, respectively. Genetic scissors, which are considered as next generation technology, are notable since foreign genes do not remain in final products.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2019년도 춘계학술대회
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• pp.367-371
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• 2019

한의학의 '과학화'는 세계 속의 K-MEDI를 위해 선행되야 할 과제로 한약의 효능을 과학적으로 입증하는 작업을 통해 유효성 및 안전성이 확보하고자 노력하고 있다. 본 논문은 한의학적 유전병변 판독을 위한 바이오마커 추출 알고리즘을 제안함으로 한의학적 관점에서 응용되있는 다양한 한의학적 치료법의 진단 및 치료 효과의 객관화에 근거를 제시하였다.

• Toxicological Research
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• 제17권
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• pp.167-171
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• 2001

The introduction of genetically modified crops has raised concerns regarding safety issues over the insertion of foreign genes into plant genomes using recombinant DNA technology. Since 1991 in Japan, 29 foods and 6 food additives have been evaluated, based on the "Guideline for Safety Assessment", before these foods were marketed. The MHW, however, decided that safety assessment of such foods and food additives should be legally imposed. because soon such foods and food additives are expected to circulate globally and a new system for assessing safety of such foods and food additives at a pre-market stage is necessary, in order to avoid the distribution of any genetically modified foods that have had no safety assessment. The MHW published relevant announcements to amend existing regulations on 1 May 2000. "Standards for safety assessment of seed plant" is established based on a concept of substantial equivalence, and applicable to the products which are regarded as equivalent to the existing products used as foods and food additives. The characterization of the food products entails consideration of the molecular characterization. phenotypic and compositional characteristics, key nutrients and toxicants, and toxicity and allergenicity of the introduced proteins, and if there are indications of unintended effects of the modification, whether further safety testing (animal studies etc.) is needed should be considered. Safety and wholesomeness studies with whole foods should be care fully designed in order to avoid nutritional imbalances causing artifacts and uninterpretable results as was the case of Dr. Pusztaiis report. A case study of genetically modified soybeans (glyphosate-tolerant soybeans) on the immune system of rats and mice is shown. Chemical compositions were also compared with those of the non-GM soybeans. The studies failed to detect any differences in immuno-toxic activity.muno-toxic activity.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1605-1614
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• 2022

The strains associated with foodborne Salmonella enterica Thompson outbreaks in Korea have not been identified. Therefore, we characterized S. Thompson strains isolated from chocolate cakes linked to foodborne outbreaks in Korea. A total of 56 strains were isolated from preserved cake products, products in the supply chain distribution, the manufacturer's apparatus, and egg white liquid products used for cream preparation. Subsequently, serological typing, pathogenic gene-targeted polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), and whole-genome multi-locus sequence typing (wgMLST) were performed to characterize these isolates. The antigen formula of all isolates was 7:k:1,5, namely Salmonella enterica subsp. enterica Serovar Thompson. All 56 isolates harbored invA, his, hin, and stn, and were negative for sefA and spvC based on gene-targeted PCR analyses. Based on PFGE results, these isolates were classified into one group based on the same SP6X01.011 pattern with 100% similarity. We selected 19 strains based on the region and sample type, which were subjected to wgMLST. Although the examined strains showed 100% similarity, they were classified into seven clusters based on allelic differences. According to our findings, the cause of these outbreaks was chocolate cake manufactured with egg white liquid contaminated with the same Salmonella Thompson. Additionally, comparative analysis of wgMLST on domestic isolates of S. Thompson from the three outbreaks showed genetic similarities of over 99.6%. Based on the results, the PFGE and wgMLST combination can provide highly resolved phylogeny and reliable evidence during Salmonella outbreak investigations.