• 한국수산과학회지
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• 제41권6호
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• pp.466-470
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• 2008

Microsatellite DNA markers were used to investigate the genetic diversity and population structure of Pacific abalone Haliotis discus hannai collected from six locations (Uljin, Ulsan, Daechon, Taean, Wando, and Yosu) where hatchery-produced abalone have been released intensively. There was no distinguishable difference in the observed and expected heterozygosities between the six populations and a cultured population. However, there was a difference in the number of alleles per locus: 12.8 for the cultured population and 13.8 to 15.8 for the six populations. The proportion of stocked abalone ranged from 41.1 to 92.7% for wild-caught populations with a decreasing tendency of alleles per locus for an increasing proportion of stocked abalone. A departure from Hardy-Weinberg equilibrium (HWE) assessed using the Markov chain procedure (P<0.05) was observed in the six populations and cultured population at loci Hdh145 and Hdh5l2. The pairwise Fst test (P<0.05) showed a significant difference between the Uljin and Ulsan populations and four remaining populations (Wando, Daechon, Yosu, and the cultured population), among which the Wando population differed less than the other three populations (Daechon, Yosu, and the cultured population).

• 사상체질의학회지
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• 제14권2호
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• pp.90-97
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• 2002

Sasang Constitutional Medicine is based on the diversity of human being and medically developed the variation of response to diseases and medicines. The diversity is categorized as four groups Taeyangin, Taeumin, Soyangin, Soeumin according to morphology, physiology, pathology, and pharmacology. The phenotypes of Sasang constitutions represent that constitutions may be possessed of the different genetic backgrounds. To clarify the genetic difference among the Sasang constitutions, we performed a genetic analysis with the 3'-UTR polymorphism of ADPRT (rs=8679) as a pooled DNA sequencing method. ADPRT modulates various nuclear proteins by poly(ADP-ribosy)lation and is involved in the regulation of various cellular processes such as differentiation, proliferation, and tumor transformation. This gene is also involved in the recovery of cell from DNA damage and the brain infarction. The allele frequencies of [T/C] polymorphism of ADPRT of Soeumin and Soyangin groups were (T: 0.94/C: 0.06) and that of Taeumin and Taeyangin groups were (T: l.00/C: 0.00). The allele frequency was not showed the difference between constitution groups. This result represented that the [T/C] polymorphism of ADPRT 3' UTR region was not suitable to classify the constitutions. However, this study is the first trial of Sasang classification according to genetic polymorphism and further analysis will be necessarily to classify the genetic difference of Sasang constitution.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.435-440
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• 2017

The author performed PCR-based genetic platform to measure the hierarchical dendrogram of Euclidean genetic distances of Korean scallop populations (KSP), particularly for Chlamys farreri, which was further compared with those of the Chinese scallop populations (CSP), by employing the with specifically designed oligonucleotide primer sets. The scallop is economically and ecologically very important bivalves in South Korea. Relatively, individuals of KSP population were fairly distantly related to that of CSP population, as shown in the hierarchical dendrogram of Euclidean genetic distances. Comparatively, individuals of KSP population were fairly distantly related to that of CSP population. Thus analysis of genetic difference between scallop populations could provide important statistics for fishery and aquaculture. Overall the results showed specific and/or conserved genetic loci between scallop populations. Information on the genetic distance of the bivalve would be helpful to understand scallop expansion or conservation in the coastal regions of South Korea. Specific markers developed by the author will be useful for the analysis of scallop population genetics and distribution in coastal region.

• 한국연초학회지
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• 제13권2호
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• pp.59-65
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• 1991

There was no a difference of genetic analysis among methods(means, joint scaling test, 3 Parameter model) . The magnitude of additive effects generally paralleled the magnitude of difference between parental means and appeared to be more independent from non-allelic interaction than did dominance effects, whereas the magnitude of dominance effects were inflated by non-allelic interaction. Additive effects were significant for all characteristics observed and it was a major effects in inheritance of number of leaves. Dominance effects were higher than additive effects for plant height, days to flower, flesh leaf weight per plant, curing rate, total alkaloid and total nitrogen.

• Reproductive and Developmental Biology
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• 제38권3호
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• pp.123-127
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• 2014

This study was conducted for SNPs in the 5'-regions of estrogen receptor-${\alpha}$ (ESR-${\alpha}$), and association with calving interval (CI), service per conception (SPC) and 305 days milk yield in Hanwoo and Holstein dairy cattle. The genetic improvement was incurred low reproduction performance. The objective of this study was to investigate connections between single nucleotide polymorphisms (SNP) of Estrogen receptor-${\alpha}$ (ESR-${\alpha}$) with reproduction performance (calving interval, service per conception, and 305 d milk yield) in Hanwoo and Holstein dairy cattle. Hanwoo and Holstein blood samples were collected from 183 and 124 dam of breeding farms and DNA was extracted. Primer design was based on NCBI GenBank (Accession No. AY340579). The PCR-RFLP method with Bgl I was used to genotype the cattle. The result showed two variants of the ESR-${\alpha}$ gene. The Bgl I cut the 492 bp amplification product into 322 bp and 170 bp fragments for allele G, while allele A remained uncut, resulting in two restriction fragments for homozygote G/G and three fragments for heterozygote A/G. We found two of different genotypes in these breeds, A/G and G/G. In Hanwoo, the A/G genotype frequency was 0.13, and G/G was 0.87. The CI of A/G was $382.18{\pm}10.03$ days, and G/G was $381.69{\pm}5.22$ days. The SPC of A/G was $1.62{\pm}0.16$, and G/G was $1.32{\pm}0.04$. While CI showed no significance difference, SPC exhibited significant difference (p<0.05). In Holstein cattle, the frequency of genotype A/G was 0.02 and G/G was 0.98. The 305 days milk yield of A/G was $7,253.00{\pm}936.00kg$ and of G/G was $8,747.51{\pm}204.88kg$, showing no significant difference.

• 한국군사과학기술학회지
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• 제20권2호
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• pp.272-278
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• 2017

In this paper, we consider a single-channel amplitude comparison monopulse system(SCACMS). The monopulse ratio curve(MR-C) of the SCACMS can be controlled by an amplitude difference between sum and different signal, a phase difference and the coefficient of the signal processor. We first propose the SCACMS with multiple variables, and then apply a genetic algorithm to optimize the multiple variables in terms of minimizing a root mean square error. The simulation results show that when three variables of the SCACMS are jointly optimized, the linear region of the MR-C can be extended approximately 187 % compared to that of two variables.

• 한국산림과학회지
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• 제102권1호
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• pp.122-128
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• 2013

우리나라에 분포하는 가침박달 9개 집단의 유전다양성과 유전구조를 ISSR 표지자를 이용하여 분석하였다. 선발된 6개 ISSR primer에서 다형성 band는 35개로 primer당 평균 5.8개(S.D.=2.32), 집단별 다형적 유전자좌의 비율은 평균 78.7%로 나타났다. AMOVA에서 전체 유전변이의 27.8%는 집단간 차이에 기인하며, 72.2%는 집단내 개체 간 차이로 설명할 수 있었다. 베이즈 방법에 따른 유전분화는 ${\theta}^{11}$와 $G_{ST}$가 각각 0.249와 0.227로 추정되었으며, 전체 집단에 대한 근친교배율은 0.412로 계산되었다. 집단간의 지리적 거리와 유전적 거리에 대한 상관성 분석에서 지리적 거리가 멀수록 유전적으로 상이한 것으로 나타났다. 베이즈 군집분석에서 가침박달 집단은 유전변이 분포에 따라서 1) 대구 지역의 2집단 및 안동, 청송, 예천 집단이 하나의 구역으로, 그리고 2) 단양, 영월 집단과 3) 임실, 청주 집단이 각각 하나의 구역으로 묶여서 총 3개 구역으로 나눌 수 있었다. 구역의 유전변이는 백두대간과 정맥의 산줄기를 경계로 분포하는 것으로 생각되며, AMOVA에서 전체 유전변이량의 10.0%는 구역간, 19.7%는 집단간, 나머지 70.3%는 집단내 개체간 차이로 설명되었다. 아울러 가침박달의 현지내 유전자원보존을 위한 유전다양성 평가와 유전구조 분석결과의 적용에 대하여 살펴보았다.

• 한국산림과학회지
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• 제103권1호
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• pp.51-58
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• 2014

본 연구는 ISSR 표지자를 이용하여 국내 분포하는 황벽나무 7개 집단의 유전다양성과 유전구조를 분석하였다. 6개의 ISSR primer를 이용하여 분석한 결과 primer 당 평균 4.5개의 다형성 band를 확인하였고, 각 집단의 다형성 유전자좌의 비율은 평균 78.8%로 나타났다. Shannon의 유전다양성 지수(I)는 0.421로 나타났고, 이형접합체 기대치($H_e$)는 평균 0.285로 베이즈 방법을 이용한 평균 이형접합체 기대치(hs=0.287)와 유사하였다. AMOVA에서 전체 유전변이의 92.4%가 집단내 개체간 차이에 기인하며, 7.6%는 집단간 차이에 기인하였다. 베이즈 방법을 이용한 유전분화(${\theta}^{II}$)는 0.066으로 추정되었으며, 전체 집단의 근친교배율(f)은 0.479로 계산되었다. 유연관계 분석과 베이즈 군집분석결과 우리나라 황벽나무 집단은 가평, 화천, 봉평, 용평이 하나의 군집을 형성하였고, 산청 지역의 2개 집단(삼장 및 시천)이 다른 하나의 군집을 형성하였으며, 무주 집단이 산청지역의 집단과 지리적으로 근접함에도 불구하고 독립적인 군집을 나타내었다. Mantel's test 결과 집단간 유전적 유연관계와 지리적 분포의 상관성은 나타나지 않았다. 황벽나무의 유전자원보존을 위한 대상 집단 선정 시 생태적 및 생활사적 특징과 함께 본 연구결과에서 나타난 유전다양성과 군집구조 분석결과를 고려하는 것이 효과적일 것으로 사료된다.

• Journal of Life Science
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• 제12권1호
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• pp.9-13
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• 2002

Crepidiastrum lanceolatum ($H_{OUTT}$) $N_{AKAI}$ (Compositae) is distributed in East Asia including Korea. Genetic diversity and population structure of six C. lanceolatum populations in Korea and two populations in Japan were determined using genetic variation at 19 allozyme loci. The percent of polymorphic loci within the enzymes was 42.1%. Genetic diversity at the species level and at the population level was low (Hes : 0.077; Hep : 0.068, respectively), where-as the extent of the population divergence was relatively low ($G_{ST}$ : 0.093). One of the most striking features of this study was the more significant difference within populations than among populations. An indirect estimate of the number of migrants per generation (Nm : 2.44) indicated that gene flow was moderate among eight populations of the species. In addition, analysis of fixation indices revealed a slight heterozygosity deficiency in some populations and at some loci. Narrow geographic ranges, short-lived perennial herbaceous, and small population sizes are mainly associated with the low level of genetic variation.n.

• 한국전자파학회:학술대회논문집
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• 한국전자파학회 2002년도 종합학술발표회 논문집 Vol.12 No.1
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• pp.278-282
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• 2002

In this paper, a dual-band CPW antenna is designed with the aid of Genetic Algorithm and Finite-Difference Time-Domain method. The design goal is to minimize the magnitude of S$\sub$11/ in the frequency band between 1.8㎓ and 2.4㎓. The resonance frequencies are shifted by about 80MHz and 50MHz from the desired frequencies at 1.8㎓ and 2.4㎓ respectively. But the bandwidth agrees well with that of Simulation.