• Horticultural Science & Technology
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• v.35 no.3
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• pp.323-332
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• 2017

Homology-specific transcriptional and post-transcriptional silencing, an intrinsic mechanism of gene regulation in most eukaryotes, can be induced by anti-sense, co-suppression, or hairpin-based double-stranded RNA. Hairpin-based RNA interference (RNAi) has been applied to analyze gene function and genetically modify crops. However, RNAi vector construction usually requires high-cost cloning steps and large amounts of time, or involves methods that are protected by intellectual property rights. We describe a more effective method for generating intron-spliced RNAi constructs. To produce intron-spliced hairpin RNA, an RNAi cassette was ligated with the first intron and splicing sequences of the Brassica rapa ssp. pekinensis histone deacetylase 1 gene. This method requires a single ligation of the PCR-amplified target gene to SpeI-NcoI and SacI-BglII enzyme sites to create a gene-specific silencing construct. We named the resulting binary vector system pKHi and verified its functionality by constructing a vector to silence DIHYDROFLAVONOL 4-REDUCTASE (DFR), transforming it into tobacco plants, and confirming DFR gene-silencing via PCR, RT-qPCR, and analysis of the accumulation of small interfering RNAs. Reduction of anthocyanin biosynthesis was also confirmed by analyzing flower color of the transgenic tobacco plants. This study demonstrates that small interfering RNAs generated through the pKHi vector system can efficiently silence target genes and could be used in developing genetically modified crops.

• The Transactions of the Korea Information Processing Society
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• v.6 no.3
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• pp.781-791
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• 1999

DD(Decision Diagrams) is an efficient operational data structure for an optimal expression of boolean functions. In a graph-based synthesis using DD, the goal of optimization decreases representation space for boolean functions. This paper represents boolean functions using OPKFDD(Ordered Pseudo-Kronecker Functional Decision Diagrams) for a graph-based synthesis and is based on the number of nodes as the criterion of DD size. For a property of OPKFDD that is able to select one of different decomposition types for each node, OPKFDD is variable in its size by the decomposition types selection of each node and input variable order. This paper proposes a method for generating OPKFDD efficiently from the current BDD(Binary Decision Diagram) Data structure and an algorithm for minimizing one. In the multiple output functions, the relations of each function affect the number of nodes of OPKFDD. Therefore this paper proposes a method to decide the input variable order considering the above cases. Experimental results of comparing with the current representation methods and the reordering methods for deciding input variable order are shown.

• The Journal of Information Technology
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• v.3 no.3
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• pp.57-65
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• 2000

We have proposed BIST method and circuit for embedded 16M SDRAM with logic. It can test the AC parameter of embedded 16M SDRAM using the BIST circuit capable of detecting the address of a fail cell of a 16M SDRAM installed in an Merged Memory with Logic(MML) generating the information of repair for redundancy circuit. The function and AC parameter of the embedded memory can also be tested using the proposed BIST method. The total gate of the BIST circuit is approximately 4,500 in the case of synthesizing by $0.25\mu\textrm{m}$ cell library. and verify the result of Verilog simulation. The test time of each one AC parameter is about 200ms using 2Y-March 14N algorithm.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.4
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• pp.849-855
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• 2021

The purpose of this study is to analyze and compare Islamic commercial banks and Islamic banking units with the stochastic frontier analysis (SFA) method during 2014-2018. The data in research using Islamic commercial banks and Islamic banking units. There are 10 Islamic commercial banks and 5 Islamic banking units that meet the criteria of purposive sampling. The calculation of efficiency level using the SFA method with the function of production shows that Islamic commercial banks and Islamic banking units always experience an increase in efficiency every year with the average level of efficiency of Islamic commercial banks being 0.43994, while the average rate of efficiency of Islamic banking units is slightly higher at 0.47654. This shows that Islamic banking units are slightly more optimal in generating total financing in the period 2010-2014. The test results using Independent Sample T-Test can be concluded that there is no difference in the efficiency value between Islamic commercial banks and Islamic banking units. Operating costs are not significant and have a positive effect on the total financing; total assets have a significant effect and a positive impact on total financing; labor costs are not significant and have a negative effect on total financing.

• Current Optics and Photonics
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• v.7 no.2
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• pp.127-135
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• 2023

Radially polarized beams with the ability to generate a sub-wavelength sized spot in a longitudinal field provides significant applications in microscopic imaging, optical tweezers, lithography and so on. However, this excellent property can also be achieved based on conventional circularly polarized beams. Here, we demonstrate its ability to create a strong longitudinal field by comparing the tight focusing characteristics of fractional-order vortex modulated radial polarized and left-handed circular polarized Bessel-Gauss beams. Additionally, the possibility of generating arbitrary fractional-order vortex modulated Bessel-Gauss beams with a strong longitudinal field is demonstrated. A special modulation method of left-handed circularly polarized Bessel-Gauss beams modulated by a fractional-order vortex is adopted creatively and a series of regulation laws are obtained. Specifically, the fractional-order phase modulation parameter n can accurately control the number of optical lobes. The ratio of the pupil radius to the incident beam waist β₁ can control the radius of the optical lobes. The first-order Bessel function amplitude modulation parameter β₂ can control the number of layers of optical lobes. This work not only adds a new modulation method for optical micromanipulation and optical communication, but also enriches the research on fractional vortex beams which has very important academic significance.

• Journal of Positioning, Navigation, and Timing
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• v.13 no.1
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• pp.63-73
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• 2024

NeQuick G is the ionosphere model utilized by Galileo single-frequency users to estimate the ionospheric delay on each user-satellite link. The model is characterized by the effective ionization level (Az) index, determined by a modified dip latitude (MODIP) and broadcast coefficients derived from daily global space weather observations. However, globally fitted Az coefficients may not accurately represent ionosphere within local area. This study introduces a method for regional ionospheric modeling that searches for locally optimized Az coefficients. This approach involves fitting TEC output from NeQuick G to TEC data collected from GNSS stations around Korea under various ionospheric conditions including different seasons and both low and high solar activity phases. The optimized Az coefficients enable calculation of the Az index at any position within a region of interest, accounting for the spatial variability of the Az index in a polynomial function of MODIP. The results reveal reduced TEC estimation errors, particularly during high solar activity, with a maximum reduction in the RMS error by 85.95%. This indicates that the proposed method for NeQuick G can effectively model various ionospheric conditions in local areas, offering potential applications in GNSS performance analyses for local areas by generating various ionospheric scenarios.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.12
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• pp.83-88
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• 2008

The QPP interleaver has been gaining attention since it provides contention-free interleaving functionality for high speed parallel turbo decoders. In this paper we first show that the quadratic term $f_2x^2%K$ of $f(x)=(f_1x+f_2x^2)%K$, the address generating function, is periodic. We then introduce a low-power address generator which utilizes this periodic characteristic. This generator follows the conventional method to generate the interleaving addresses and also to save the quadratic term values during the first half of the first period. The saved values are then reused for generating further interleaved addresses, resulting in reduced number of logical operations. Power consumption is reduced by 27.38% in the design with fixed-K and 5.54% in the design with unfixed-K on average for various values of K, when compared with the traditional designs.

• Korean Journal of Remote Sensing
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• v.17 no.1
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• pp.45-56
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• 2001

To acquire seabed information, the mosaic images of the seabed were generated using Side Scan Sonar. Short time energy function which is needed for slant range correction is proposed to get the height of Tow-Fish to the reflected acoustic amplitudes of each ping, and that leads to a mosaic image without water column. While generating mosaic image, maximum value, last value and average value are used for the measure of a pixel in the mosaic image and 3-D information was kept by using acoustic amplitudes which were heading for specific direction. As a generating method of mosaic image, low resolution mosaic image which is over 1m/pixel resolution was generated for whole survey area first, and then high resolution mosaic image which is generated under 0.1m/pixel resolution was generated for the selected area. Rocks, ripple mark, sand wave, tidal flat and artificial fish reef are found in the mosaic image.

• Journal of the Institute of Electronics Engineers of Korea TE
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• v.39 no.3
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• pp.97-104
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• 2002

In this paper we describe the method which optimizes the partition of the input space by means of measure of fuzziness for fuzzy neural network. It covers its generation of fuzzy rules for input sub space. It verifies the performance of the system depended on the various time interval of the input. This method divides the input space into several fuzzy regions and assigns a degree of each of the generated rules for the partitioned subspaces from the given data using the Shannon function and fuzzy entropy function generating the optimal knowledge base without the irrelevant rules. In this scheme the basic idea of the fuzzy neural network is to realize the fuzzy rule base and the process of reasoning by neural network and to make the corresponding parameters of the fuzzy control rules be adapted by the steepest descent algorithm. According to the input interval the proposed inference procedure proves that the fast convergence of root mean square error (RMSE) owes to the optimal partition of the input space

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.1
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• pp.1-11
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• 2016

The domestic and foreign seismic design codes have specified that time history covers design response spectrum when the response spectrum, which calculated from the time history, is smaller than the design response spectrum at five points or less. In order to verify the design codes, time history analysis for a pier was performed by using five artificial time histories conforming design code with various characteristics and its member forces were evaluated according to them. It was confirmed from analysis results that, regardless of the conformity to design code requirement, seismic design using the artificial time histories could not guarantee earthquake resistant design if the response spectrum from them is lower than design response spectrum at the similar period to the natural frequency of structure. Thus, the time history generating method to make its acceleration response spectrum to be greater than design response spectrum at all period was proposed by two modification function in this study. It was also verified whether time histories from the proposed method satisfy the seismic design codes or not.