• 제목/요약/키워드: gel-type

검색결과 966건 처리시간 0.025초

Rhodosporidium toruloides의 접합형 A 세포내 Invertase의 정제 (Purification of Internal Invertase in Rhodosporidium toruloides Mating Type A Cells)

  • 정영기;류병호
    • 한국식품영양과학회지
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    • 제21권6호
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    • pp.725-730
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    • 1992
  • 이담자 효모균 Rhodosporidium toruloides의 mating type A 세포에서 세포내 invertase를 정제하였다. 세포내 invertase는 배양 세포의 파쇄액을 산침전 시킨 후 그 상등액으로부터 DEAE-Sephadex A-50, SP-Sephadex C-50 column chromatography와 Sephadex G-200 gel fitration 등의 과정을 거쳐 polyacrylamide gel disc 전기영동상 단일 효소 단백질까지 정제되었다. 정제효소의 분자량은 gel filtration에 의하여 90,000이었고, SDS-PAGE상에서는 22,000 dalttons에서 단일 band를 보여 단일종의 subunit가 4개로 구성된 단백질로 추정된다.

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Tissue engineering of dental pulp on type I collagen

  • Lee, Gwang-Hee;Huh, Sung-Yoon;Park, Sang-Hyuk
    • Restorative Dentistry and Endodontics
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    • 제29권4호
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    • pp.370-377
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    • 2004
  • The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 ($1{\;}{\times}{\;}10^5{\;}cells/ml/well$) were cultured at 12-well plate at $37^{\circ}C$ for 14 days. Standardized photographs were taken with digital camera during 14 days and the diameter of the contracted collagen gel matrix was measured and statistically analyzed with student t-test. As one of the pulp tissue engineering, normal human dental pulp tissue and collagen gel matrix cultured with dental pulp cells for 14 days were fixed and stained with Hematoxyline & Eosin. According to this study, the results were as follows: 1. The contraction of collagen gel matrix cultured with pulp cells for 14 days was significantly higher than other fibroblasts (gingiva, skin) (p < 0.05), 2. The diameter of collagen gel matrix cultured with pulp cells was reduced to 70.4% after 7 days, and 57.1% after 14 days. 3. The collagen gel without any cells did not contract, whereas the collagen gel cultured with gingiva and skin showed mild contraction after 14 days (88.1% and 87.6% respectively). 4. The contraction of the collagen gel cultured with NIH 3T3 cells after 14 days was higher than those cultured with gingival and skin fibroblasts, but it was not statistically significant (72.1%, p > 0.05). 5. The collagen gel matrix cultured with pulp cells for 14 days showed similar shape with native pulp tissue without blood vessels. This approach may provide a means of engineering a variety of other oral tissue as well and these cell behaviors may provide information needed to establish pulp tissue engineering protocols.

Electric and Electrochemical Characteristic of PMMA-PEO Gel Electrolyte for Rechargeable Lithium Battery

  • 박수길;박종은;이홍기;이주성
    • 한국전기전자재료학회논문지
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    • 제11권10호
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    • pp.768-772
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    • 1998
  • The new type polymer electrolyte composed of polymethyl methacrylate(PMMA) - polyethy leneoxide(PEO) contain $LiClO_4$ -EC/PC was developed for the weightless and long or life time of lithium polymer batery system with using polyaniline electrode. the gel type electrolytes were prepared by PMMA with PEO at different lithium salts in the glove box. The minimum thickness of PMMA-PEO gel electrolyte for the slim type is about(400~450$\mu\textrm{m}$. These gel electrolyte showed good compatibility with lithium electrode. The test cell Li/polymer electrolyte/polyaniline solid state cell which was prepared by different lithium salt was researched by electrochemical technique.

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콘크리트 구조물의 내구성 증진을 위한 침투성 발수제의 성능평가 (Evaluation on Performance of Penetrative Water-repellent for Durability Progress of Concrete Structure)

  • 장석환;우종태;남용혁;김성수
    • 한국구조물진단유지관리공학회 논문집
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    • 제7권1호
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    • pp.289-296
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    • 2003
  • The evaluation on performance of gel type penetrative water-repellent for durability progress of concrete structure has carried out in this study. And liquid type waterproof agents have been estimated with same method. Also normal concrete that was not applied water-repellent and waterproof agents have been estimated. As results, the penetrative depth of gel type water-repellent were deeper than that of liquid type waterproof agents and those were superior to these in physical and durable performance.

유기 다공성 소재의 흡유 효과 (Oil Absorption Effects of Organic Porous Materials)

  • 강영구;한상범
    • 한국안전학회지
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    • 제21권1호
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    • pp.86-91
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    • 2006
  • Oil spills caused by the accidents have been occurred from house and factory waste, grounded tanker, the rupture of storage tank and oil pipelines, the deterioration of various industrial facilities, etc. Many oil spills result in contamination of shorelines and workplace. Fire and explosion may happen from these spills. There are several technologies used for clean-up application, which include use of oil dispersing agents, absorbents, solidifiers, booms and skimmers by physical, chemical, and biological methods. Methods for oil spill clean-up operation are classified into the absorption type, gel type and self-swelling type. Porous materials with oil absorptive properties are classified into micropore, mesopore, and macropore depending on their pore sizes. Recently, new porous materials with smaller size have been developed, but the selective oil absorption in water-in-oil interface demonstrates the macro pore size. In this study oil absorption effects were evaluated using the organic porous materials with a complex function of gel type and swelling type. Samples were subjected to analysis by FT-IR spectroscopy and were characterized in terms of gel formation and morphologies. Oil sorption capacity, pressure retention force and gel strength were also measured. From these results, the physicochemical reactivity before and after gelation was verified and the industrial applications of clean-up operation were suggested.

Preparation of Unsaturated Polyester-based Hybrid Gel-Coats Containing Urethane Acrylate and Their Coating Performance

  • Kim, Ji-Hee;Baek, Seung-Suk;Kim, Oh Young;Park, Dong Hyup;Hwang, Seok-Ho
    • Elastomers and Composites
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    • 제54권3호
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    • pp.247-251
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    • 2019
  • Two different urethane acrylates (mono-acrylate and di-acrylate) were used to prepare unsaturated polyester-based hybrid gel-coats. The physical properties and surface characteristics of these gel-coats were investigated on the basis of the content and type of urethane acrylate. The set-to-touch time increased and the physical properties (surface hardness and tensile strength) decreased with an increase in the urethane acrylate content. However, the type of urethane acrylate did not affect these parameters. It was found that the optimal urethane acrylate content for the application of unsaturated polyester-based hybrid gel-coats is ~10 wt%.

분질고구마 대유미 전분의 이화학적 및 겔 특성 (Physicochemical and Gel Properties of Starch Purified from Mealy Sweet Potato, Daeyumi)

  • 정온빛;윤희나;노준희;김욱;신말식
    • 한국식품조리과학회지
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    • 제32권4호
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    • pp.524-530
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    • 2016
  • Purpose: The properties of starch and starch gel prepared from a newly inbred sweet potato, Daeyumi were compared to the properties of starch and starch gel prepared from Sinyulmi which is a well known mealy type sweet potato. Methods: The starch was isolated by using the alkaline steeping method. Physicochemical, pasting, and thermal properties, and crystallinity were measured. The texture properties of starch gel (10%, w/w) were examined. Results: The amylose contents of Daeyumi and Sinyulmi starches were 25.57% and 22.59%, respectively. The initial pasting temperature of Daeyumi starch was significantly higher than that of Sinyulmi starch (p<0.05), but other paste viscosities were not different. The peak and conclusion temperatures of Daeyumi starch were higher than those of Sinyulmi starch by differential scanning calorimetry. The shape of Daeyumi starch gel was more clear and rigid than the shape of Sinyulmi starch gel. The surface and the upper side of Daeyumi starch gel were smoother than the surface and the upper side of Sinyulmi starch gel. Hardness and gumminess were higher in Daeyumi starch gel than in Sinyulmi starch gel. The crystallinity types of Daeyumi and Sinyulmi starches were $C_b$ and A types, respectively, but starch gels showed an amorphous type. Conclusion: Therefore, it is suggested that Daeyumi starch would have better physicochemical properties and higher quality of starch gel than Sinyulmi starch.

PLGA 다공성 지지체에 함침시킨 DBP젤의 연골재생 효과: In Vivo 실험 (Effect of Demineralized Bone Particle Gel Penetrated into Poly(lactic-co-glycolic acid) Scaffold on the Regeneration of Chondrocyte: In Vivo Experiment)

  • 이윤미;심초록;이유정;김하늘;조선아;송정은;이동원;강길선
    • 폴리머
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    • 제36권6호
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    • pp.789-794
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    • 2012
  • 생체적합성 및 생분해성의 장점을 지닌 PLGA는 우수한 기계적 성질과 분해속도를 조절할 수 있는 장점을 가지고 있지만 소수성으로 인해 세포의 초기부착률이 낮고, 지지체 내부에 영양액의 공급이 원활하지 않다. 본 연구에서는 이러한 단점을 보완하고자 탈미네랄화된 골분(DBP, demineralized bone particle) 젤을 PLGA 지지체에 함침시켰다. 다양한 형태의 지지체 압축강도를 측정한 결과 PLGA/DBP 지지체가 연골과 비슷한 강도를 가지고 있었으며, 이를 바탕으로 DBP젤을 함침시킨 PLGA/DBP젤 지지체를 제작하였다. 연골세포를 파종하여 in vivo 실험을 진행하였고 조직화 정도를 확인하기 위해 H&E, Safranin-O, Alcian blue, Collagen type I 및 Collagen type II 염색을 실시하였다. 그 결과 DBP를 함침시킨 PLGA 지지체에서 GAG, Collagen type I 및 Collagen type II의 높은 발현과 조직화를 보여 연골조직으로 대체할 수 있는 가능성을 확인하였다.

한국 재래 및 야생종 콩의 Leucine Aminopeptidase 변이 (Variation of Leucine Aminoeptidase Isozyme in Korean Land Races and Wild Soybeans)

  • 박경숙;윤문섭
    • 한국작물학회지
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    • 제42권2호
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    • pp.129-133
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    • 1997
  • 한국 재래종 대두(Glycine max) 943계통과 강원도 지성 수집종 야생종(G.soja) 50계통을 polyacrylamide gel electrophoresis와 isoelectric focusing 전기영동으로 LAP 동립효소를 분석한 결과를 요약하면 다음과 같다. PAGE에 의한 Lap1*b는 재래종과 야생종에서 가장 흔한 대립인자였다. Lap1*b의 대립인자 빈도는 한국 야생종(0.94)에서보다 재래종(1.00)에서 더 높았다. 이러한 결과는 한국 재래종 대두는 Lap1 유부자좌에서 Lap1*b 대립인자로 고정되어 있음을 나타내는 것이다. IEF 전기영동법을 이용하여 pH 4∼6.5의 gel에서 LAP 동립효소의 band type을 확인한 결과 재래종과 야생종에서 I type과 II type의 band type을 확인하였다. 재래종과 야생종에서 II type이 6계통(7.2%)과 4계통(8.0%)으로 각각 나타났고 I type은 79계통(92.8%)과 46계통(92.0%)으로 각각 나타났다. 이러한 결과는 다양한 band type이 나타날 수 있는 가능성을 제시하고 있다.

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