• Title/Summary/Keyword: gel-type

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Evaluation of Gelation Properties of Salt-Soluble Proteins Extracted from Protaetia brevitarsis Larvae and Tenebrio molitor Larvae and Application to Pork Myofibrillar Protein Gel System

  • Ji Seon Choi;Geon Ho Kim;Ha Eun Kim;Min Jae Kim;Koo Bok Chin
    • Food Science of Animal Resources
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    • v.43 no.6
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    • pp.1031-1043
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    • 2023
  • The purpose of this study was to investigate the functional properties of salt-soluble proteins obtained from Protaetia brevitarsis (PB) and Tenebrio molitor (TM) larvae, the interaction between these proteins and pork myofibrillar protein (MP) in a gel system. The gel properties of salt-soluble protein extracts showed that the PB had a higher viscosity than the TM protein. However, the TM protein had higher gel strength compared with the PB protein. The gelation characteristics of the pork MP gel systems added with lyophilized insect salt-soluble protein powder showed to decrease slightly viscosity compared with MP alone. Adding the TM or PB protein powder did not affect the pork MP's hydrophobicity and sulfhydryl group levels. Furthermore, the protein bands of the MP did not change with the type or amount of insect salt-soluble protein. The cooking yields of the pork MP gels containing PB or TM protein powder were higher than those without insect protein. Regardless of the type of insect salt-soluble protein added, the pork MP's gel strength decreased. Furthermore, as the level of insect powder increased, the surface protein structure became rough and porous. The results demonstrated that proteins extracted from PB and TM larvae interfered with the gelation of pork MP in a gel system.

Transient Characteristic of a Metal-Oxide Semiconductor Field Effect Transistor in an Automotive Regulator in High Temperature Surroundings

  • Kang, Chae-Dong;Shin, Kye-Soo
    • Transactions on Electrical and Electronic Materials
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    • v.11 no.4
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    • pp.178-181
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    • 2010
  • An automotive IC voltage regulator which consists of one-chip based on a metal-oxide semiconductor field effect transistor (MOSFET) is investigated experimentally with three types of packaging. The closed type is filled with thermal silicone gel and covered with a plastic lid on the MOSFET. The half-closed type is covered with a plastic case but without thermal silicone gel on the MOSFET. Opened type is no lid without thermal silicone gel. In order to simulate the high temperature condition in engine bay, the operating circuit of the MOSFET is constructed and the surrounding temperature is maintained at $100^{\circ}C$. In the overshoot the maximum was mainly found at the half-closed packaging and the magnitude is dependent on the packaging type and the surrounding temperature. Also the impressed current decreased exponentially during the MOSFET operation.

In vitro Angiogenic Activity of Aloe vera Gel on Calf Pulmonary Artery Endothelial (CPAE) Cells

  • Lee, Myoung-Jin;Lee, Ok-Hee;Yoon, Soo-Hong;Lee, Seung-Ki;Chung, Myung-Hee;Park, Young-In;Sung, Chung-Ki;Choi, Jae-Sue;Kim, Kyu-Won
    • Archives of Pharmacal Research
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    • v.21 no.3
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    • pp.260-265
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    • 1998
  • Angiogenic activity of Aloe vera gel was investigated by in vitro assay. We obtained the most active fraction from dichloromethane extract of Aloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type I collagen gel and form capillary-like tube through in vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel through in vitro invasion assay. Furthermore, the effect on the MRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitory (PAl-1) mRNA was not changed.

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Studies on the Serological Classification for Korean aspergilli (한국산 Aspergilli의 혈청학적 분류방법)

  • 문희주;이배함
    • Korean Journal of Microbiology
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    • v.12 no.4
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    • pp.180-187
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    • 1974
  • Of the Asp. spp. isolated by the Institute of Applied Microbiology, Kon-Kuk University, 7 strains were selected for the study of the immunological differencences among them using gel precipitation test. The strains were the following types : 1 type of flavus and 2 types of oryzae were isolated from Meju ; 1 type of flavus from Nuruk ; and each one type of flavus, parasiticus and oryzae from Kokja.Asp.flavus from ATCC, Asp. parasiticus nad Asp. niger NRRL strains were also used in the study as a standard. From this study, several points can be raised ; 1) There was no common antigenic property between Asp. niger and Asp. flavus, because of no formation of reaction line. Therefore, all strains could be easily distinguished. 2) There was common antigenic property, that is, the formation of reaction line between Asp. flavus and Asp. parasticus. Accordingly two strains could not be easily distinguished by the gel precipitation test. 3) Each type of oryzae, parasiticus and flavus of Asp. flavus group had common antigen one another as well as specific antigens only in the difference of the reaction lines, so they could be easily identified in the gel precipitation test. 4) Each isolated strain from Meju and Nuruk appeared to be identical. 5) It was shown that the gel precipitation test of serological methods was very useful for the classification of Asp. spp.

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Studies on the Physiological Chemistry of the Spring Habits in Naked Barley IV. Electrophoretic Variations in Seed Proteins during Ripening (과맥의 파성에 대한 생리화학적 연구 제4보 등숙과정에 있어서의 종자단백질의 전기영동특성 변이)

  • 최선영;이강수
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.1
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    • pp.55-59
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    • 1982
  • With naked barley varieties which are different in their spring habits, variations in seed proteins during ripening were checked by acrylamide gel electrophoresis. In case of 7% acrylamide gel among protein bands ⅰ-band was observed only in both Sedohadaka (intermediate type) and Wanju (spring type) throughout the ripening period, and j-band was detected in all the three varieties until 33 days after heading, but not in Nonsangwa (winter type) thereafter. In case of 6M urea-7% acrylamide gel z-band was traced only in Nonsangwa, contrary to i-band, throughout the ripening period. U-band was observed in all the three varieties until 23 days after heading, but not in Nonsangwa thereafter. X-band showed opposite from u-band. Throughout the ripening period Sedohadaka was significantly more similar in the electrophoretic patterns to Wanju than to Nonsangwa.

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Study on the Gel Cleaning System for Removal of Poly (vinyl acetate) Fixative of the Mural Paintings of the Payathonzu Temple in Bagan, Myanmar (I) - Focusing on Properties and Removability of Gel Cleaners -

  • Yu, Yeong Gyeong;Han, Gyu-Seong;Lee, Hwa Soo;Han, Kyeong Soon
    • Journal of Conservation Science
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    • v.37 no.4
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    • pp.370-379
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    • 2021
  • In the past, PVAc [poly(vinyl acetate)] was used as a fixative for the conservation of the murals in the murals of the Payathonzu temple located in Bagan, Myanmar. In this study, attention was paid to gel cleaning as a method for stably removing such PVAc fixatives. Based on the recent research trend related to the cleaning of murals in Bagan ruins, 3 types of gelling agents (Nevek®, Laponite®RD, Carbopol®980) and 2 types of organic solvents (Acetone, Dimethyl carbonate) were selected. Six types of gel cleaners were prepared by mixing gelling agents and organic solvents, and the properties and fixative removability of these cleaners were compared. As a result of confirming the properties of the prepared gel cleaners, the pH of the cleaners was all in the weak acidic to weakly alkaline range, which was a stable condition for mural application. Also, there was no difference in the viscosity of the cleaners depending on the type of solvent, but there was a difference depending on the type of gelling agent used. Regarding the weight loss ratio of PVAc, which is an indicator of removability, the exposure conditions of the gel cleaners, the boiling point of the solvent used, and the viscosity of the gelling agent acted as factors affecting. As a result of comparing the removability of gel cleaners, it was confirmed that the solvent's fixative solubility, the volatility of the solvent itself, and the solvent release control properties of the gelling agent had a great effect on the removability of the gel cleaners. In Part 2, the stability and the running applicability of the gel cleaners will be investigated by making mockup samples reflecting the properties of the materials and techniques used to produce the mural paintings in the Payathonzu Temple.

Optimum Levels of Flavoring Materials for Gel-type Dessert Using Chicken-feet Gelatin (닭발 젤라틴을 이용한 후식용 gel 제조를 위한 향신료의 최적수준)

  • Lim, Ju-Yeon;Jang, Eun-Gyung;Kim, Kwang-Ok
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.911-915
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    • 2002
  • This study was conducted to determine optimum levels of flavoring materials for gel-type dessert using chicken feet gelatin. Effects of levels of sucrose, citric acid and strawberry flavoring on the consumer acceptability of gelatin desserts were examined and the optimum levels were determined using response surface methodology. The optimum levels of flavoring materials determined for gelatin dessert containing 2% gelatin powder, were 19, 0.50, and 0.35% for sucrose, citric acid, and strawberry flavoring, respectively.

Accuracy of Root ZX in teeth with simulated root perforation in the presence of gel or liquid type endodontic irrigant

  • Shin, Hyeong-Soon;Yang, Won-Kyung;Kim, Mi-Ri;Ko, Hyun-Jung;Cho, Kyung-Mo;Park, Se-Hee;Kim, Jin-Woo
    • Restorative Dentistry and Endodontics
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    • v.37 no.3
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    • pp.149-154
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    • 2012
  • Objectives: To evaluate the accuracy of the Root ZX in teeth with simulated root perforation in the presence of gel or liquid type endodontic irrigants, such as saline, 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine liquid, 2% chlorhexidine gel, and RC-Prep, and also to determine the electrical conductivities of these endodontic irrigants. Materials and Methods: A root perforation was simulated on twenty freshly extracted teeth by means of a small perforation made on the proximal surface of the root at 4 mm from the anatomic apex. Root ZX was used to locate root perforation and measure the electronic working lengths. The results obtained were compared with the actual working length (AWL) and the actual location of perforations (AP), allowing tolerances of 0.5 or 1.0 mm. Measurements within these limits were considered as acceptable. Chi-square test or the Fisher's exact test was used to evaluate significance. Electrical conductivities of each irrigant were also measured with an electrical conductivity tester. Results: The accuracies of the Root ZX in perforated teeth were significantly different between liquid types (saline, NaOCl) and gel types (chlorhexidine gel, RC-Prep). The accuracies of electronic working lengths in perforated teeth were higher in gel types than in liquid types. The accuracy in locating root perforation was higher in liquid types than gel types. 5.25% NaOCl had the highest electrical conductivity, whereas 2% chlorhexidine gel and RC-Prep gel had the lowest electrical conductivities among the five irrigants. Conclusions: Different canal irrigants with different electrical conductivities may affect the accuracy of the Root ZX in perforated teeth.

Simulant Gel Propellant Characteristics depending on Mixing Method (제작방법에 따른 모사 젤 추진제의 특성 연구)

  • Kim, Jae-Woo;Jun, Doo-Sung;Shin, Woong-Sup;Lee, Hyo-Mi;Moon, Hee-Jang
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.467-470
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    • 2011
  • In this study, two different kind of impeller, commercial hand blender and manual type were used to investigate the most effective mixing method for simulant gel propellant. Ionized Water, Carbopol 941 and NaOH were used to produce the simulant gel for temperature of $25^{\circ}C$ and $50^{\circ}C$. The amount of bubbles produced during mixing of simulant gel at $50^{\circ}C$ were higher than that of simulant gel at $25^{\circ}C$. After 24 hours, bubbles of simulant gel made at $50^{\circ}C$ disappeared rapidly with respect to the bubbles of gel made at $25^{\circ}C$. Bubbles from blender did show notable amount even after 24 hours. Among mixing type, it was found that the pitched paddle impeller was the best candidate for the production of simulant gel.

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