• Archives of Plastic Surgery
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• v.51 no.1
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• pp.14-19
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• 2024

Background Hyaluronic acid fillers can be manufactured using various processes. They have multiple properties, including their concentration, degree of modification, and rheological data. Cohesion is one such property to evaluate gel integrity; however, there is no standardized method for calculating this parameter. This study aimed to evaluate different tests for calculating hyaluronic acid cohesion and discuss the importance of hyaluronic acid cohesion as a consideration when selecting fillers. Methods The cohesion levels of five different hyaluronic acid fillers with different rheological properties were evaluated and compared using the drop weight, compression, tack, and dispersion time tests. Results The cohesion tests yielded different results in the samples. Samples 2 and 4 showed approximately two times the number of droplets when compared with Sample 5 in drop weight test. Samples 1, 2, 3, and 4 were superior to Sample 5 in tack test. Samples 1, 2, and 3 showed cohesive appearances at 95 seconds in most cases in dispersion test. Rheological test results did not reflect the measures of cohesion. Conclusion Although there are no definite standardized tests to evaluate the cohesion of hyaluronic acid fillers, our proposed tests showed similar results for different hyaluronic acid filler products. Further studies are needed to evaluate the cohesion of hyaluronic acid fillers and determine the clinical use of this distinguishing characteristic for clinicians selecting the product of choice. Level of evidence statement: These data are Level IV evidence.

• Culinary science and hospitality research
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• v.15 no.4
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• pp.99-114
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• 2009

This study examined the optimal cooking condition for surimi-like material (SLM) derived from porcine semimembranosus (SM) muscle and the effects of the various cooking temperatures and the cooking time on the gel properties. The most noticeable change that occurred during the preparation of the SLM from the SM was the reduction in the fat content (about 1%) during the washing procedures. The hardness and gel strength value were increased significantly as the cooking temperature was increased by $75^{\circ}C$ for 20 min (p<0.05). The SLMG cooked above $75^{\circ}C$ had a significantly higher WHC than the SLMG cooked below $75^{\circ}C$ (p<0.05). The gelling property of SLMG was effected for different conditions of cooking time and temperatures by the result of SDS-PAGE. After 20 min cooking, some enzyme bands including phosphorylase disappeared. The loss of these bands (about 46 kDa and 60 kDa) was observed after 20 min of cooking time. The photographs of microscopy showed that the filaments of myofibrils did not disappear after a cooking time of 15 min, and that the gaps between the fibers or filament were close. A significant change in the fibers and filaments occurred from 30 min to 35 min of cooking time, and the gradual coagulation of the structure of the SLM was observed with cooking time increased. These results suggest that a desirable surimi gel could be obtained from pork by cooking at $75^{\circ}C$ for 25 min.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.591-596
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• 2000

The effect of starches such as potato, corn and wheat starch on the rheological properties of ocean jumbo squid kamaboko was investigated. Changes in the water holding capacity and color values of those kamaboko gels was also studied using the maximum gel strength endowing starch, Wheat starch could ive the better water holding capacity and breaking stress than potato or corn starch within $10{\%}$ additional level but corn starch resulted the highest those value at $20{\%}$ added. Wheat starch had higher level of breaking strain and jelly strength at $10{\%}$ then in descending order were corn starch, potato starch. But those starches were decreased after $15{\%}$ level. Texture map showed the simple rheological properties of each starches heat gel with jumbo squid kamaboko, Corn starch map showed more tough and brittle than the other. Potato starch map showed more elastic gel than corn starch. Wheat starch map could make elastic-mushy gel. There was no significant color differences of each starches but the whiteness of each starch showed increase when the starch rate was increased.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.4
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• pp.309-314
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• 2002

Rhtological properties of surimi gel from white fishes by acid (acid surimi) and alkali (alkali surmi) process and effect of chemicals on gelation were investigated by punch and dynamic tests. The breaking force and deformation values of heat-induced gel of acid surimi were less than their values of alkali and conventional surimi gel, and whiteness was greatly decreased, Gel point of acid surimi was decreased but it of alkali surimi was increased with increasing moisture content in the range of 80 to $85\%$. Storage modulus of acid surimi was the highest vaule in pH 6.8, but that of alkali surimi showed high value at neutral and slightly alkali pH. Propylene glycol increased storage modulus in $20\~50^{\circ}C$, hut urea and 2-mercaptoethanol suppressed it. Potassium bromide improved storage modulus in $20~80^{\circ}C$, The results suggest that alkai process is used for making surimi instead of conventional method.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.20 no.4
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• pp.173-177
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• 2010

Thermal stabilizing effect of $Yb^{3+},\;Er^{3+}$ codoping into $TiO_2$ powder prepared by sol-gel method and its upconversion characteristics were analyzed. The effect of $TiO_2:Yb^{3+},\;Er^{3+}$ ions on crystallinity and phase transition was studied by X-ray diffraction (XRD). The change of band-gap energy induced from Yb and Er codoping was analyzed by UV-Vis. The band-gap energy of $TiO_2$ have been slightly narrowed by $Yb^{3+},\;Er^{3+}$ codoping, which indicated that the $Yb^{3+},\;Er^{3+}$ ions can enhance the photo-catalytic property of $TiO_2$. green and red up-conversions of $Yb^{3+}$ and $Er^{3+}$ co-doped $Y_2O_3:Yb^{3+},\;Er^{3+}$ phosphor were analyzed by PL equipped with 980 nm laser.

• Clean Technology
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• v.14 no.3
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• pp.204-210
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• 2008

A series of $V_{2}O_{5}-TiO_2$ xerogel catalysts were prepared by nonhydrolytic sol-gel method and analysed by various characterization techniques. These catalysts showed much higher surface areas and total pore volumes than conventional V$V_{2}O_{5}-TiO_2$ xerogel and impregnated $V_{2}O_{5}/TiO_2$ catalysts. It was found that the textural property of $V_{2}O_{5}-TiO_2$ material varies with the method and conditions of synthesis. Surface vanadates and $TiO_2$ anatase phase are the crucial factors to obtain high catalytic activities. The selective oxidation of hydrogen sulfide in the presence of excess water and ammonia was studied over these catalysts. Xerogel catalysts prepared by non-hydrolytic sol-gel method showed very high conversion of $H_{2}S$ without harmful emission of $SO_2$. The highest catalytic activity shown by these $V_{2}O_{5}-TiO_2$ catalysts may be due to their high surface area and good dispersion of vanadia species in the titania matrix.

• Clean Technology
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• v.22 no.4
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• pp.258-268
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• 2016

In this work, carboxymethylcellulose-based composite superabsorbent hydrogels were prepared by electron beam radiation. The composition of carboxymethylcellulose (CMC) varied from 4 wt%, 5 wt%, and 6 wt% to 7 wt% based on the amount of distilled water in the syntheses of hydrogels. Graphite oxide, reduced graphene oxide, activated carbon, and bentonite were used as additives for the synthesis of composite superabsorbent. The effect of CMC composition and the type of additives on the gel properties of the prepared hydrogels was investigated. In order to verify the functional groups in the prepared materials, Fourier transform infrared spectroscopy was used. In addition, mechanical strength, gel fraction, swelling kinetics, and equilibrium swelling ratios were measured for the prepared hydrogels. Swelling experiments were carried out in distilled water, urea solution, and physiological saline water. Prepared hydrogels were reused for 5 times, and gel fraction and swelling ratio were measured at every 24 hours. Among the prepared hydrogels, $C_{5%}GO$ and $C_{5%}rGO$ exhibited excellent mechanical property and relatively high swelling ratios for urea solution and physiological saline water with promising applicability as slow-release fertilizers.

• Journal of Microbiology and Biotechnology
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• v.32 no.4
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• pp.531-540
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• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

• Clean Technology
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• v.15 no.4
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• pp.258-264
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• 2009

Thermodynamic measurements were performed to show the possibility of recovering $CO_2$ from fuel gas (the mixture of $CO_2$ and $H_2$) by forming gas hydrates with water where water was dispersed in the pores of silica gel particles having nominal 100 nm of pore diameter. The hydrate-phase equilibria for the ternary $CO_2+H_2$+water in pores were measured and $CO_2$ concentrations in vapor and hydrate phase were determined under the hydrate-vapor two phase region at constant 274.15 K. It was shown that the inhibition effect appeared due to silica gel pores, and the corresponding equilibrium dissociation pressures became higher than those of bulk water hydrates at a specific temperature. In addition, direct measurement of $CO_2$ content in the hydrate phase showed that the retrieved gas from the dissociation of hydrate contained more than 95 mol% of $CO_2$ when 42 mol% of $CO_2$ and balanced Hz mixture was applied. Compared with data obtained in case of bulk water hydrates, which showed just 83 mol% of $CO_2$ where 2-stage hydrate slurry reactor was intended to utilize this property, the hydrate formation in porous silica gel has enhanced the feasibility of $CO_2$ separation process. Hydrate formation as not for slurry but solid particle makes it possible to used fixed bed reactor, and can be a merit of well-understood technologies in the industrial field.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.216-220
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• 2002

1,4-Benzoquinone reductase was purified to electrophoretic homogeneity from bovine liver, and the purified enzyme found to have a molecular mass of 29 kDa, as determined by sodium dodecyl sulfate- polyacrylamide gel electrophoresis The enzyme exhibited pH optimum between 8.0 and 8.5. The apparent fm for 1,4-benzoqulnone was 1.643 mM, and the apparent Km for NADH was 1.837 mM. Various divalent cations, such as Hg$\^$2+/, Cu$\^$2+/, and Zn$\^$2+/, exhibited strong inhibitory effects. The enzyme activity was also strongly inhibited by quercetin, dicumarol, and benzoic acid. Incubation of the enzyme with N-bromosuccinimide and pyridoxal 5’-phosphate led to inhibitions of 100% and 99%, respectively. Accordingly, these results suggest that trypto-phan and Iysine residues are Involved at or near the active sites of the enzyme.