• Title/Summary/Keyword: gel analysis

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WICK DERIVATIONS ON WHITE NOISE FUNCTIONALS

  • Chung, Dong-Myung;Chung, Tae-Su
    • Journal of the Korean Mathematical Society
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    • v.33 no.4
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    • pp.993-1008
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    • 1996
  • The white noise analysis, initiated by Hida [3] in 1975, has been developed to an infinite dimensional distribution theory on Gaussian space $(E^*, \mu)$ as an infinite dimensional analogue of Schwartz distribution theory on Euclidean space with Legesgue measure. The mathematical framework of white noise analysis is the Gel'fand triple $(E) \subset (L^2) \subset (E)^*$ over $(E^*, \mu)$ where $\mu$ is the standard Gaussian measure associated with a Gel'fand triple $E \subset H \subset E^*$.

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Texture profile analysis of acorn flour gel-Comparison of 3$\times$3 latin square with 3sup 3 factorial experiment - (도토리묵의 Texture 특성 -라틴방격법과 요인배치법의 비교-)

  • 김영아
    • Journal of the Korean Home Economics Association
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    • v.23 no.3
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    • pp.49-53
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    • 1985
  • The typical texture profile analysis of acorn flour gel was investigated with Instron univ. testing machine by two experimental designs, 3$\times$3 latin square and $3^{3}$factorial experiment. As the result, it was revealed that Latin square is a useful method to reduce the number of experiments, in the case of a negligible interaction.

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Glucose Analysis Using Free and Immobilized Glucose Oxidase Electrode (고정화 효소전극을 이용한 포도당분석)

  • Jang, Ho-Nam;Ju, Dae-Gwon;Kim, Yeong-Seong
    • Journal of Biomedical Engineering Research
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    • v.3 no.2
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    • pp.95-100
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    • 1982
  • Glucose oxidase from A. niger was entrapped in polyacrylamide gel which was used in the enzyme electrode for glucose analysis. The electrode was assembled by placing the gel between the membranes on the surface of a Clark type electrode. In order to make it possible to analyze the experimental results later, the stagnation flow was adopted wheree the governing fluid mechanics were well known. The current increased with the increase concentration in the bulk below a certain level of glucose concentration beyond which no more current increase was observed. This is probably due to the diffusion limitation of oxygen from the bulk solution. Also the current increased witll the enzyme loading in the gel, but the linearity between the current and the glucose concentration was rather limited to a narrow range. Flow rate was found to be very important, which means that film diffusion is very important under the flow rate of 5cm/sec. As a conclusion, enzyme loading, gel layer thickness, stirring speed and bulk concentration of glucose were found to be most improtant parameters in yielding a linar current reponse with respect to the bulk glucose concentration.

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Surimi Quality from Mechanically Deboned Chicken Meat as Affected by Washing Cycle, Salt Concentration, Heating Temperature and Rate

  • Min, Byung-Jin;Lee, Sung-Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.1
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    • pp.131-136
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    • 2004
  • The effects of salt concentration and heating conditions on the thermal gelation properties of surimi produced from mechanically deboned chicken meat (MDCM) were investigated. Chicken surimi was manufactured by washing (MDCM: 0.5% NaCl=1:4), standing, straining and centrifuging. The fat, water-soluble protein and heme pigment in the MDCM were removed by increasing washing cycles. The compressive force of the chicken surimi increased as the concentration of salt was increased from 0% to 5%. Total gel strength of the surimi measured by texture profile analysis showed a maximum in the range 3-5% NaCl. Microstructural analysis showed that the unfolding network structure of the surimi gel began to appear at NaCl concentrations>2%. The optimum heating condition for gelation was $90^{\circ}C$ for 40 min as this resulted in maximum values for measures of gel strength including compressive force, hardness, fracturability, adhesiveness, springiness, gumminess, chewiness and resilience. Chicken surimi gel formed by cooking at a heating rate of $1^{\circ}C/min$ to $90^{\circ}C$ showed better a texture than gels produced at $1.85^{\circ}C/min$. Our result show that a lower rate of heating improves chicken surimi gelation.

Relationship Between Acrylamide Concentration and Enzymatic Activity in An Improved Single Fibrin Zymogram Gel System

  • Choi, Nack-Shick;Kim, Byoung-Young;Lee, Jin-Young;Yoon, Kab-Seog;Han, Kyoung-Yoen;Kim, Seung-Ho
    • BMB Reports
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    • v.35 no.2
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    • pp.236-238
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    • 2002
  • Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDS-fibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10kDa to over 100kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.

Lane Detection and Tracking Algorithm for 3D Fluorescence Image Analysis (3D 형광이미지 분석을 위한 레인 검출 및 추적 알고리즘)

  • Lee, Bok Ju;Moon, Hyuck;Choi, Young Kyu
    • Journal of the Semiconductor & Display Technology
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    • v.15 no.1
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    • pp.27-32
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    • 2016
  • A new lane detection algorithm is proposed for the analysis of DNA fingerprints from a polymerase chain reaction (PCR) gel electrophoresis image. Although several research results have been previously reported, it is still challenging to extract lanes precisely from images having abrupt background brightness difference and bent lanes. We propose an edge based algorithm for calculating the average lane width and lane cycle. Our method adopts sub-pixel algorithm for extracting rising-edges and falling edges precisely and estimates the lane width and cycle by using k-means clustering algorithm. To handle the curved lanes, we partition the gel image into small portions, and track the lane centers in each partitioned image. 32 gel images including 534 lanes are used to evaluate the performance of our method. Experimental results show that our method is robust to images having background difference and bent lanes without any preprocessing.

Rheological Model Analysis of Acorn Starch Gels by Stress Relaxation Test (응력완화시험에 의한 도토리 전분겔의 물성론적 모형 분석)

  • 김영아;이혜수
    • Korean journal of food and cookery science
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    • v.5 no.1
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    • pp.49-52
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    • 1989
  • Stress relaxation tests with different percent deformation were performed for crude and refined starch gels of acorn. With no relation to percent deformation, refined starch gel had higher initial stress ($\sigma_e$) and lower equilibrium stress ($\sigma_e$) than crude starch gel. But the ratio of equilibrium stress to initial stress ($\sigma_e$/$\sigma_o$) was minimum at 60 percent deformation. The analysis of relaxation curves by successive residual method revealed that the rheological behavior of acorn starch gels could be expressed by generalized Maxwell model. The element numbers of models for crude and refined starch gel were 7-element and 5-element at 60 percent deformation, and 5-element and 3-element at 45 percent deformation, respectively.

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A comparative study of 3D printing and sol-gel polymer production techniques: A case study on usage of ABS polymer for radiation shielding

  • Hasan Ogul;Batuhan Gultekin;Fatih Bulut;Hakan Us
    • Nuclear Engineering and Technology
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    • v.56 no.6
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    • pp.1943-1949
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    • 2024
  • This study focuses on the comparative analysis of ABS polymer samples produced using two distinct manufacturing techniques: 3D printing and the sol-gel methods. In the first approach, ABS polymer was augmented with rare earth oxides, Er2O3 and Gd2O3, in nano powder form and fabricated into test specimens using 3D printing technology. In the second approach, identical samples were prepared via the sol-gel technique involving mold-based fabrication. Elemental content analysis revealed no significant differences between the samples produced by the two methods. The study proceeds to evaluate the gamma-ray shielding, neutron shielding, temperature resistance, and SEM/EDS pictures of ABS samples generated through both techniques. 3D printing method exhibited more favorable results in terms of structure morphology and thermal stability while there is no significant difference for radiation shielding. The results provide insights into the performance and suitability of each production method for radiation shielding applications. This research not only contributes to enhancing radiation shielding technology but also informs the selection of the most appropriate fabrication method for specific applications in nuclear technologies and diagnostic energy range in medical purposes.

A Case Study of Concrete Pavement Deterioration by Alkali-Silica Reaction in Korea

  • Hong, Seung-Ho;Han, Seung-Hwan;Yun, Kyong-Ku
    • International Journal of Concrete Structures and Materials
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    • v.1 no.1
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    • pp.75-81
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    • 2007
  • The concrete pavement of the Seohae Highway in Korea has suffered from serious distress, only four to seven years after construction. Deterioration due to Alkali-Silica Reaction (ASR) has seldom been reported per se in Korea, because the aggregate used for the cement concrete has been considered safe against alkali-silica reaction so far. The purpose of this study is to examine the deterioration caused by an alkali-silica reaction of concrete pavement in Korea. The investigation methods included visual inspection and Automatic Road Analyzer (ARAN) analysis of surface cracks, coring for internal cracks, stereo microscopic analysis, scanning electronic microscope (SEM) analysis, and electron dispersive X-ray spectrometer (EDX) analysis. The results are presented as follows: the crack pattern of the concrete pavement in Korea was longitudinal cracking, map cracking or D-cracking. Local areas of damage were noticed four to five years after construction. The cracks started from edges or joints and spread out to slabs. The most intensive cracking was observed at the intersection of the transverse and longitudinal joints. Where cracking was the most intense, pieces of concrete and aggregate had spalled away from top surface and joint interface area. The progress of deterioration was very fast. The reaction product of alkali-silica gel was clearly identified by its generally colorless, white, or very pale yellow hue seen through a stereo optical microscopy. The typical locations of the reaction product were at the interface between aggregate and cement paste in a shape of a rim, within aggregate particles in the cracks, and in the large void in the cement paste. Most of the white products were found at interface or internal aggregates. SEM and EDX analysis confirmed that the white gel was a typical reaction product of ASR. The ASR gel in Korea mainly consisted of Silicate (Si) and Potassium (K) from the cement. The crack in the concrete pavement was caused by ASR. It seems that Korea is no longer safe from alkali-silica reaction.

Gel Electrophoresis Analysis of the Hard Coronas of Human Serum Albumin on Silica Nanoparticles: Size Dependence of Corona Formation

  • Kim, Sung-Jong;Han, Sang Yun
    • Bulletin of the Korean Chemical Society
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    • v.35 no.9
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    • pp.2621-2624
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    • 2014
  • The rapid and spontaneous adsorption of proteins on nanoparticle (NP) surfaces in biological fluids such as blood is an important phenomenon as it possibly determines "what the cells see" and, thus, the fates of NPs in living organisms. In order to quantitatively understand protein coronas at the molecular level, we investigated human serum albumin (HSA) coronas that were produced on silica NPs of 20 nm and 50 nm diameters using conventional gel electrophoresis. Analysis of the concentration dependence of protein adsorption showed that HSA coronas preferentially formed a monolayer on silica NPs and revealed the presence of hard protein coronas. HSA adsorption was clearly dependent on NP size, and this might be due to the different surface curvatures of NPs of different sizes.