• Environmental Analysis Health and Toxicology
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• v.20 no.4 s.51
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• pp.297-302
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• 2005

The purpose of this study was to provide the standard method for the analysis of organophosphorous pesticides such as chlorpyrifos, diazinon, malathion and parathion in blood. We performed method validation for these pesticides in blood according to EURACHEM (A focus For Analytical Chemistry in Europe) guide. For the analysis of the pesticides, we used solid-phase extraction ,column (Waters Oasis $HLB^{(R)}$. After the extraction, the supernatants were evaporated to dryness under the nitrogen stream. They were analyzed by gas chromatography/mass spectrometry (GC/MS) after reconstituting with ethanol. Terbufos was used as an internal standard. To validate this method, we performed verification procedures with the following parameters: selectivity, linearity of calibration, accuracy, precision, limit of detection and quantification. Validation data according to Eurachem guide were adequate for our purpose for the analysis of chlorpyrifos, diazinon, malathion and parathion in blood.

• Journal of the Korean Chemical Society
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• v.38 no.10
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• pp.726-733
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• 1994

Supercritical fluid extraction (SFE) followed by gas chromatographic separation and mass spectrometric (MS) detection were used in rapid analysis of polycyclic aromatic hydrocarbons (PAH's) in air particulate material extracted for 30 min with 10 ml of supercritical $N_2O$ without another sample preparation step. Two samples, urban dust in Seoul area and a certified air particulate reference material 1649 supplied by the NBS (National Bureau of Standards), were processed for the purpose of evaluating extraction and analysis methods. As a result, the quantitative recovery of PAH's in the SFE method was relatively lower than conventional organic solvent extraction methods, but reproducibility was resonable, and analysis time was reduced remarkably. The method has proved to be suitable for monitoring of PAH's in air particulate material.

• Analytical Science and Technology
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• v.7 no.1
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• pp.41-52
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• 1994

The importance of separation comes from demands on study for exact effect of synthetic drugs and the reactivity of enantiomer in biological system. Racemization rate was measured under the influence of heat, acid, UV-light, enzyme(trypsin) and 6N-HCl at $105^{\circ}C$ on alanine, threonine, isoleucine, lecuine, aspartic acid, methionine, glutamic acid, tyrosine. The method for the identification of overlapped amino acids with GC was developed from the close study of fragmentation pattern with mass spectrometry. With cyclodextrin bonded phase by HPLC, the separation of dansyl amino acid was tested for compartison.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1523-1531
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• 2012

The metabolite profile of meju during fermentation was analyzed using mass spectrometry techniques, including GC-MS and LC-MS, and the bacterial diversity was characterized. The relative proportions of bacterial strains indicated that lactic acid bacteria, such as Enterococcus faecium and Leuconostoc lactis, were the dominant species. In partial least-squares discriminate analysis (PLS-DA), the componential changes, which depended on fermentation, proceeded gradually in both the GC-MS and LC-MS data sets. During fermentation, lactic acid, amino acids, monosaccharides, sugar alcohols, and isoflavonoid aglycones (daidzein and genistein) increased, whereas citric acid, glucosides, and disaccharides decreased. MS-based metabolite profiling and bacterial diversity characterization of meju demonstrated the changes in metabolites according to the fermentation period and provided a better understanding of the correlation between metabolites and bacterial diversity.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.45-49
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• 2006

Fumigant toxicity of four plant essential oils (muguet flower, patchouli, pennyroyal, spearmint) were tested against the adults of western flower thrips, Frankliniella occidentalis. Among them, pennyroyal and spearmint oils showed 100% mortality at $1{\mu}{\ell}/45m{\ell}$ air dose. Through the constituent analysis using GC and GC-MS, we confirmed that main constituents of pennyroyal oil were pulegone(100.0%), and spearmint oil were $\beta-myrcene(3.5%)$, limonene(12.1%), carvone(85.4%). Among them, carvone and pulegone showed 100% fumigation at $1{\mu}{\ell}/45m{\ell}$ air dose, respectively. It can concluded that two oils are potential control agents against F. occidentalis.

• Korean journal of food and cookery science
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• v.14 no.5
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• pp.541-546
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• 1998

Volatile flavor components in leaf and petiole of fresh Pleurospermum kamtschaticum H$\_$OFFM/ were extracted by SDE (simultaneous steam distillation and extraction) method using diethyl ether as solvent. Essential oils were analyzed by gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS). Identification of volatile flavor components was based on the Rl of GC and mass spectrum of GC-MS. A total of 31 components, including 15 hydrocarbons, 4 aldehydes, 1 ketone, 5 alcohols, 2 esters, 3 acids and 1 oxide were identified in the essential oils. (Z)-${\beta}$-Farnesene, (Z, E)-${\alpha}$-farnesene and farnesene were the major volatile flavor components in fresh Pleurospermum kamtschaticum. Volatile flavor patterns of Pleurospermum kamtschaticum were analyzed using electronic nose. Sensor T30/1 and PA2 that were sensitive to alcohols had the highest resistance for fresh Pleurospermum kamtschaticum. Resistance of six metal oxide sensors was decreased in dried sample compared with fresh one.

• Applied Biological Chemistry
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• v.31 no.3
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• pp.292-297
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• 1988

The volatiles of whole and homogenated tomato fruits collected by the headspace trapping method using Tenax GC and the simultaneous steam distillation method were identified by GC and GC-MS. Among over 100 GC peaks, 10 alcohols, 6 aldehydes, 4 ketones, 3 esters, 1 phenol and 1 acid were identified from whole tomato fruits, whereas 12 alcohols, 6 aldehydes, 5 ketones, 5 esters, 2 phenols, 1 hydrocarbon and 1 acid were identified from homogenated tomato fruits. By simultaneous steam distillation-extraction, 19 alcohols, 13 hydrocarbons, 9 esters, 9 ketones, 8 aldehydes, 2 phenols, 2 lactones,2 furans, 1 acids and 2 others were identified among over 300 peaks.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.189-194
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• 2006

Fumigant toxicity of 18 plant essential oils were tested against the adults of oak longicorn beetle, Moechotypa diphysis. Among them, eucalyptus, rosemary and pennyroyal oils showed 100% mortality and sage oil showed 85% mortality at 10 $\mu\ell/\ell$ (air) dose. Eucalyptus and rosemary oils showed 100% mortality within 6 hr after treatment at 10 $\mu\ell/\ell$ (air) dose. GC and GC/MS analysis of the four essential oils and bioassay of their components revealed that 1,8-cineole (a major component of eucalyptus, rosemary and sage oils), thujone (a major component of sage oil) and pulegone (a major component of pennyroyal oil) showed higher adulticidal activity than others.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.120.2-121
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• 2003

The alkylphenols, chlorophenols and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) mode followed by two work-up methods for comparison; isoBOC derivatization method and TBDMS derivatization method. Eleven phenols in paper samples were extracted with acetonitrile. (omitted)

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2001.11a
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• pp.348-349
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• 2001

휘발성유기화합물 (VOCs)은 복잡 다양한 발생원에서 배출되고 있다. 1990년에 개정된 미국 공기청정법 (US Clean Air Act Amendments, CAAA)에서는 총 189개의 화합물을 인간환경에 유해한 유해 대기 오염물질 (HAP, hazordous air pollutants)로 분류하고 있다 (MacKensie, A.R 등, 1991). 이 중 미국 EPA에서는 유해 대기오염물질로서 43개의 VOCs를 정하여, TO-14 화합물로서 일컫고 있다 (허귀석, 1999). (중략)