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Method Validation for the Simultaneous Analysis of Organophosphorous Pesticides in Blood by GC/MS  

Park Mee Jung (Forensic Science Department, National Institute of Scientific investigation)
Yang Ja Youl (Forensic Science Department, National Institute of Scientific investigation)
Kim Ki Wook (Forensic Science Department, National Institute of Scientific investigation)
Park Yoo Shin (Forensic Science Department, National Institute of Scientific investigation)
Chung Hee Sun (Forensic Science Department, National Institute of Scientific investigation)
Lee Sang Ki (Forensic Science Department, National Institute of Scientific investigation)
Publication Information
Environmental Analysis Health and Toxicology / v.20, no.4, 2005 , pp. 297-302 More about this Journal
Abstract
The purpose of this study was to provide the standard method for the analysis of organophosphorous pesticides such as chlorpyrifos, diazinon, malathion and parathion in blood. We performed method validation for these pesticides in blood according to EURACHEM (A focus For Analytical Chemistry in Europe) guide. For the analysis of the pesticides, we used solid-phase extraction ,column (Waters Oasis $HLB^{(R)}$. After the extraction, the supernatants were evaporated to dryness under the nitrogen stream. They were analyzed by gas chromatography/mass spectrometry (GC/MS) after reconstituting with ethanol. Terbufos was used as an internal standard. To validate this method, we performed verification procedures with the following parameters: selectivity, linearity of calibration, accuracy, precision, limit of detection and quantification. Validation data according to Eurachem guide were adequate for our purpose for the analysis of chlorpyrifos, diazinon, malathion and parathion in blood.
Keywords
organopholphorous pestirides; method validation; lolid-phase extrartion; CC/MS;
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