• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.1013-1018
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• 2009

The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be $60.7{\pm}4.9%$. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of $140{\pm}7.9%$ and $108{\pm}3.5%$, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately $65{\pm}0.6%$.

• Archives of Plastic Surgery
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• 제33권2호
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• pp.245-248
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• 2006

Besides gastric pull-up or colonic interposition, microvascular technique in esophageal reconstruction has been approved reliable methods. When free intestinal transfer is considered, jejunal free flap is commonly used. We treated the patient who had undergone reconstruction with a right colon interposition and suffered from inability of swallowing because of stricture and necrosis of the interposed flap. Although we have planned jejunal free transfer, we couldn't use jejunum due to adhesion by previous gastrojejunostomy and colon interposition. Salvage procedure with microvascualr free left colon flap was executed successfully. After 9 month follow-up, the patient was able to consume a normal diet.

• Journal of Gastric Cancer
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• 제19권1호
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• pp.121-131
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• 2019

Purpose: The significance of neuroendocrine differentiation (NED) in gastric carcinoma (GC) is controversial, leading to ambiguous concepts in traditional classifications. This study aimed to determine the prognostic threshold of meaningful NED in GC and clarify its unclear features in existing classifications. Materials and Methods: Immunohistochemical staining for synaptophysin, chromogranin A, and neural cell adhesion molecule was performed for 945 GC specimens. Survival analysis was performed using the log-rank test and univariate/multivariate models with percentages of NED ($P_{NED}$) and demographic and clinicopathological parameters. Results: In total, 275 (29.1%) cases were immunoreactive to at least 1 neuroendocrine (NE) marker. GC-NED was more common in the upper third of the stomach. $P_{NED}$, and Borrmann's classification and tumor, lymph node, metastasis stages were independent prognostic factors. The cutoff $P_{NED}$ was 10%, beyond which patients had significantly worse outcomes, although the risk did not increase with higher $P_{NED}$. Tumors with ${\geq}10%$ NED tended to manifest as Borrmann type III lesion with mixed/diffuse morphology and poorer histological differentiation; the NE components in this population mainly grew in insulae/nests, which differed from the predominant growth pattern (glandular/acinar) in GC with <10% NED. Conclusions: GC with ${\geq}10%$ NED should be classified as a distinct subtype because of its worse prognosis, and more attention should be paid to the necessity of additional therapeutics for NE components.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.127-127
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• 2018

Probiotics are microorganisms that have beneficial effects on the health of the host. The health promoting effect by probiotics influences suppressing harmful bacteria, prevention of constipation, blood cholesterol reduction and regulation of blood pressure. Prebiotics are used to promote the growth or activity of microorganisms. Synbiotics, which are a mixture of probiotics and prebiotics, synergize in the intestines by complementing each other. Synbiotics not only improves the viability of the probiotics while passing through the gastrointestinal tract, maintain intestinal homeostasis, but also regulate balance of harmful and useful bacterial growth. Glycine max Merr (GMM) has been widely used in Asian countries to treat cancer, obesity, oxidative stress and imbalanced immune diseases. In addition, it has been reported that dietary fiber-rich grains promote bowel movements and prevent constipation. In this study, we investigated the viability of LactobacillIus buchneri (L.buchneri) strains, known as lactic acid bacteria under conditions of gastric fluid and intestinal fluid to determine the suitability of L.buchneri as probiotics. The adhesion ability of L.buchneri to caco-2 cells was also confirmed. The present studies showed that GMM extract promoted the growth and activity of L.buchneri strains as prebiotics. Also, this results suggested that the mixture of L.buchneri and GMM extract can helps maintain intestinal health and healthy body as synbiotics and health functional food material.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.531-536
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• 1998

We had previously reported that the protective effect of taurine against indomethacin-induced gastric mucosal injury was due to its antioxidant effects, which inhibited lipid peroxidation and neutrophil activation. In this study, we examined the effect of taurine on reducing the inflammatory parameters of trintrobenzene sulfonic acid (TNBS)-induced inflammatory bowel disease (IBD) in rats. In order to induce IBD, ethanolic TNBS was given to rats intracolonically. Then they received 500 mg/kg.day of taurine orally and were sacrificed one week after IBD induction. While ulceration and inflammation of distal colon with formation of granuloma in the vehicle-treated IBD rats two days after administration of TNBS were observed, treatment with taurine ameliorated colonic damage and decreased the incidence of diarrhea and adhesion. also, colon weight as an index of tissue edema, which was mardedly increased in the IBD rats, became significantly lower after administration of TNBS were observed, treatment with taurine ameliorated colonic damage and decreased the incidence of diarrhea and adhesion. Also colon weitht as an index of tissue edema, which was markedly increased in the IBD rats, became significantly lower after taruine treatment. Myeloperoxidase (MPO) activity in the vehicle-treated IBD rats was substantially increased, compared with that of normal control. the taurine-treated animals significantly reduced MPO activity (35% lower) when compared with that of the vehicle-treated animals. Taurine treatment decreased both basal and formyl-methionyl leucyl phenylalanine-stimulated reactive oxygen generation from colonic tissue in the IBD rats. These results suggest that the administration of taurine reduce the inflammatory parameters in this IBD rat model by increasing defending capacity against oxidative damage.

• 한국임상수의학회지
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• 제32권1호
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• pp.28-35
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• 2015

본 연구는 개에서 복강경 위관삽입술(LG)을 시행, 수술시간, 합병증, 유착부를 평가하여 수의분야에서 LG의 유용성을 내시경 유도하 경피 위관삽입술(PEG)에 준하여 확인하였다. 정상 비글견 8마리를 두 군으로 나누어 Tri-Funnel Replacement Gastrostomy tube (Bard Inc., USA, 20 Fr.)와 PEG kit (Ponsky "Pull" PEG Kit$^{(R)}$, Bard Inc., USA, 20 Fr.)를 이용하여 LG 및 PEG를 실시하였다. 위관을 통한 영양 공급은 유지 열량 요구량(MER)을 1일 3회로 나누어 2주간 진행하고 LG와 PEG의 수술 중, 수술 후, 사후로 나누어 평가하였다. 모든 개체에서 위관삽입은 성공적이었고, PEG 군에서의 평균 수술 시간이 LG군에 비해 유의적으로 짧았다(p < 0.05). 위-복막간 유착은 군간 모든 개체에서 양호하게 형성되었으나 평균 유착 길이(AL)와 너비(AW)의 수치가 LG군에서 PEG군에 비해 유의하게 높았다 (p < 0.05). 평균 유착 거리(AD)는 군간 차이를 보이지 않았다(p = 0.182). 본 연구를 통해 개에서 LG는 최소 침습적이면서 쉽고 안전하게, 그리고 효과적으로 장관 영양 공급을 실시할 수 있는 방법임이 확인되었다.

• 대한한의학회지
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• 제19권1호
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• pp.470-499
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• 1998

For the purpose of designing more successful cancer research, the strategy for cancer study in the field of Traditional Korean Medicine(TKM) during the 21th century was examined from the analysis of trends on cancer study in traditional korean medicine. The results were summarized as follows: 1. So far cancer research in TKM was chiefly done on cytotoxicity, side-effects by chemotherapy, tumor immunology, apoptosis, survival time with S-180 and pulmonary colonization assay and also clonogenic assay, cell adhesion assay, angiogenesis, cell-differentiation and side-effect by radiotherapy were partly performed. 2. It may be ideal that we should study synergistic effect between constituent drugs of prescriptions, tumor immunology, combined therapy between western and oriental medicines by reducing side-effect by radiotherapy and chemotherapy and antimetastasis according to the characteristics of oriental medicine chiefly and also supplement the studies on molecular biology, gene therapy, angiogensis and signal transduction. 3. We had better do specific-field research in cooperation between oriental medical colleges and Korea Institute of Oriental Medicine(KIOM) as well as study a target cancers such as hepatic cancer, pulmonary cancer and gastric cancer more intensively than all cancers domestically. 4. Our country must keep communication with China having many clinical data, Taiwan chiefly doing the combined tharapy between oriental and western medicines, Japan having done basic study actively on cancer.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.205-211
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• 2022

Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The anti-inflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factor-kappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.507-516
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• 2023

Eradication of Helicobacter pylori infection is an essential strategy to decrease the risk of developing gastric cancer. However, the standard triple therapy has negative aspects associated with side effects and the emergence of antibiotic resistance. Therefore, alternative therapies are required to enhance the management of H. pylori infection effectively. In this study we examined the effect of emodin on the amelioration of inflammatory response due to H. pylori infection. Our results indicated that emodin treatment effectively decreased the expression of virulence genes, including sabA, vacA, cagL, cagA, sabA, and suppressed the adhesion ability of H. pylori to AGS cells. Emodin has been shown inhibitory effects on the inflammasome pathway through reductions in VacA translocation, lowering ROS stress, cleaved Caspase-1, NLRP3, and cleaved Gasdermin D levels, thereby lowered pyroptosis in infected cells. In summary, our study demonstrated that emodin has the ability to attenuate inflammation caused by H. pylori by modulating virulence gene expression and decreasing VacA translocation. Further study is required to evaluate the therapeutic efficacy of emodin in treating H. pylori infection and better understand the underlying mechanisms.

• 한국식품과학회지
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• 제47권1호
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• pp.44-50
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• 2015

본 연구에서는 시판 생막걸리에서 분리한 유산균의 probiotics 특성을 연구하기 위해 내산성, 내담즙성, 장 부착능에 효능이 있는 6개 균주를 선별하여 동정하였다. 생막걸리에서 분리한 유산균을 pH 2.5의 산성 및 담즙산(0.3% oxgall) 조건에서 내산성과 내담즙성을 측정한 결과 BSM-2, BSM-3, GSM-3, TJH-1, EHJ-1, EHJ-2의 6개 균주에서 각각 $10^7$, $10^8CFU/mL$ 높은 생존률을 나타내어 우수한 내산성과 내담즙성을 확인할 수 있었다. 내산성과 내담즙성에 효능이 있는 6개 균주의 16S rDNA 분석 결과, L. plantarum (BSM-2, EHJ-1) L. casei (GSM-3, EHJ-2), L. brevis (BSM-3), P. pentosaceus (TJH-1)의 4개 group이 동정되었다. 장부착능에서는 다른 유산균에 비해 장내 부착능이 뛰어난 것으로 알려진 L. rhamnosus GG를 대조군으로 사용하였으며 HT-29 cell에 대해 6종의 유산균이 모두 장 부착능을 보였으며 L. casei(GSM-3, EHJ-2)가 가장 장 부착능이 높았다. 항산화능력은 L. plantarum BSM-2와 EHJ-1에서 각각 32.44%, 24.65%의 DPPH radical 소거능을 보였다. 시판 생막걸리에서 분리된 유산균은 다양한 식중독 원인 세균에 대하여 항균활성을 지니고 있었으며, 본 연구에서는 유산균이 생성하는 항균물질인 bacteriocin이 아닌 유산균 발효 중 젖산을 생성하면서 pH의 저하로 항균활성을 보인다는 결과를 확인할 수 있었다. Probiotics로의 기능성을 가진 6개 균주의 콜레스테롤 저하능력은 L. plantarum BSM-2와 EHJ-1에서 각각 68.82%, 56.38%의 높은 감소율을 나타내었다. 시판 생막걸리에서 분리한 유산균의 probiotic 특성을 조사해 본 결과 L. plantarum BSM-2, EHJ-1, L. brevis BSM-3, L. casei GSM-3, EHJ-2, P. pentosaceus TJH-1 모두 프로바이오틱스의 기본특성을 지니고 있어 프로바이오틱 균주로서 이용가치가 충분하다고 판단되어지며, L. plantarum BSM-2가 내산성, 내담즙성이 가장 우수한 결과를 나타내었고, 장 부착성이 우수하였으며, 뛰어난 항산화 능력과 항균력을 지니며, 콜레스테롤 저하능도 뛰어나 가장 우수한 프로바이오틱 균주라고 판단된다.