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http://dx.doi.org/10.4014/jmb.2110.10034

Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response  

Kim, Won-Ju (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Hyun, Jun-Hyun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.32, no.2, 2022 , pp. 205-211 More about this Journal
Abstract
Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The anti-inflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factor-kappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.
Keywords
Probiotics; anti-inflammatory; HT-29 cell; ERK signaling; Akt signaling;
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1 Long X, Ye Y, Zhang L, Liu P, Yu W, Wei F, et al. 2016. IL-8, a novel messenger to cross-link inflammation and tumor EMT via autocrine and paracrine pathways (review). Int. J. Oncol. 48: 5-12.   DOI
2 Hubert-Buron A, Leblond J, Jacquot A, Ducrotte P, Dechelotte P, Coeffier M. 2006. Glutamine pretreatment reduces IL-8 production in human intestinal epithelial cells by limiting IκBα ubiquitination. J. Nutr. 136: 1461-1465.   DOI
3 Yu HS, Kim WJ, Bae WY, Lee NK, Paik HD. 2020. Inula britannica inhibits adipogenesis of 3T3-L1 preadipocytes via modulation of mitotic clonal expansion involving ERK 1/2 and Akt signaling pathways. Nutrients 12: 3037.   DOI
4 Singh S, Bhatia R, Singh A, Singh P, Kaur R, Khare P, et al. 2018. Probiotic attributes and prevention of LPS-induced pro-inflammatory stress in RAW264.7 macrophages and human intestinal epithelial cell line (Caco-2) by newly isolated Weissella cibaria strains. Food Func. 9: 1254.   DOI
5 Argyri AA, Zoumpopoulou G, Karatzas KAG, Tsakalidou E, Nychas GJE, Panagou EZ, et al. 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiol. 33: 282-291.   DOI
6 Kim H, Kim HS, Park WJ, Chung DK. 2015. Inhibitory effect of Lactobacillus plantarum extracts on HT-29 colon cancer cell apoptosis induced by Staphylococcus aureus and its alpha-toxin. J. Microbiol. Biotechnol. 25: 1849-1855.   DOI
7 Wang J, Qi L, Wu Z, Mei L, Wang H. 2016. Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells. Int. J. Biol. 87: 481-487.
8 Kim WJ, Yu HS, Bae WY, Ko KY, Chang KH, Lee NK, et al. 2021. Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3-L1 preadipocytes. J. Food Biochem. 45: e13896.
9 Maurer U, Preiss F, Brauns-Schubert P, Schlicher L, Charvet C. 2014. GSK-3-at the crossroads of cell death and survival. J. Cell. Sci. 127: 1369-1378.   DOI
10 Kim HG, Lee SY, Kim NR, Ko MY, Lee JM, Yi TH, et al. 2008. Inhibitory effects of Lactobacillus plantarum lipoteichoic acid (LTA) on Staphylococcus aureus LTA-induced tumor necrosis factor-alpha production. J. Microbiol. Biotechnol. 18: 1191-1196.
11 Singh S, Singh AP, Sharma B, Owen LB, Singh RK. 2010. CXCL8 and its cognate receptors in melanoma progression and metastasis. Future Oncol. 6: 111-116.   DOI
12 Fernandes AF, Bian Q, Jiang JK, Thomas CJ, Taylor A, Pereira P, et al. 2009. Proteasome inactivation promotes p38 mitogen-activated protein kinase-dependent phosphatidylinositol 3-kinase activation and increases interleukin-8 production in retinal pigment epithelial cells. Mol. Biol. Cell 16: 3690-3699.
13 Zhu L, Dai LM, Shen H, Gu PQ, Zheng K, Liu YJ, et al. 2019. Qing Chang Hua Shi granule ameliorate inflammation in experimental rats and cell model of ulcerative colitis through MEK/ERK signaling pathway. Biomed. Pharmacother. 116: 108967.   DOI
14 Son YO, Pratheeshkumar P, Wang L, Fan J, Kim DH, Lee JY, et al. 2013. Reactive oxygen species mediate Cr(VI)-induced carcinogenesis through PI3K/AKT-dependent activation of GSK-3β/β-catenin signaling. Toxicol. Appl. Pharmacol. 271: 239-248.   DOI
15 Mendes V, Galvao I, Vieira AT. 2019. Mechanisms by which the gut microbiota influences cytokine production and modulates host inflammatory responses. J. Interferon Cytokine Res. 39: 393-409.   DOI
16 Zhang Y, Zhang Z, Wang H, Cai N, Zhou S, Zhao Y, et al. 2016. Neuroprotective effect of ginsenoside Rg1 prevents cognitive impairment induced by isoflurane anesthesia in aged rats via antioxidant, anti-inflammatory and anti-apoptotic effects mediated by the PI3K/AKT/GSK-3β pathway. Mol. Med. Rep. 14: 2778-2784.   DOI
17 Jiang Y, Li L, Sun H, Shan Y, Liu Y, Li L, et al. 2016. Induction of cytokines via NF-κB and p38 MAP kinase signaling pathways associated with the immunomodulation by Lactobacillus plantarum NDC 75017 in vitro and in vivo. J. Func. Foods 20: 215-225.   DOI
18 Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh Jahromi M, et al. 2014. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. Biomed. Res. Int. 2014: 927268-927216.
19 Li X, Hu D, Tian Y, Song Y, Hou Y, Sun L, et al. 2020. Protective effects of a novel Lactobacillus rhamnosus strain with probiotic characteristics against lipopolysaccharide-induced intestinal inflammation in vitro and in vivo. Food Funct. 11: 5799.   DOI
20 Conlon MA, Bird AR. 2015. The impact of diet and lifestyle on gut microbiota and human health. Nutrients 7: 17-44.   DOI
21 Jang HJ, Lee NK, Paik HD. 2019. Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi. Food Sci. Biotechnol. 28: 1521-1528.   DOI
22 Jeong JH, Jang S, Jung BJ, Jang KS, Kim BG, Chung DK, et al. 2015. Differential immunue-stimulatory effects of LTAs from different lactic acid bacteria via MAPK signaling pathway in RAW 264.7 cells. Immunobiol. 220: 460-466.   DOI
23 Yang SJ, Kim KT, Kim TY, Paik HD. 2020. Probiotic properties and antioxidant activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in fermented black gamju. Foods 9: 1154.   DOI
24 Issac Ginsburg. 2002. Role of lipoteichoic acid in infection and inflammation. Lancet Infect. Dis. 2: 171-179.   DOI
25 Song MW, Chung Y, Kim KT, Hong WS, Chang HJ, Paik HD. 2020. Probiotic characteristics of Lactobacillus brevis B13-2 isolated from kimchi and investigation of antioxidant and immune-modulating abilities of its heat-killed cells. LWT-Food Sci. Technol. 128: 109452.   DOI
26 Gupta T, Kaur H, Kapila S, Kapila R. 2021. Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli-induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κB signaling. J. Appl. Microbiol. https://doi: 10.1111/jam.15153.   DOI
27 Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD. 2019. Anti-inflammatory potential of probiotic strain Weissella cibaria JW15 isolated from kimchi through regulation of NF-κB and MAPKs pathways in LPS-induced RAW 264.7 cells. J. Microbiol. Biotechnol. 29: 1022-1032.   DOI
28 Guo CF, Zhang S, Yuan YH, Yue TL, Li JY. 2015. Comparison of lactobacilli isolated from Chinese suan-tsai and koumiss for their probiotic and functional properties. J. Funct. Foods 12: 294-302.   DOI
29 Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD. 2019. Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15. Food Sci. Biotechnol. 28: 851-855.   DOI
30 Kim KT, Yang SJ, Paik HD. 2021. Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities. Food Sci. Biotechnol. 30: 257-265.   DOI
31 Yu HS, Jang HJ, Lee NK, Paik HD. 2019. Evaluation of the probiotic characteristics and prophylactic potential of Weissella cibaria strains isolated from kimchi. LWT-Food Sci. Technol. 112:108229.   DOI
32 Bao Y, Zhang Y, Zhang Y, Liu Y, Wang S, Dong X, et al. 2010. Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control 21: 695-701.   DOI
33 Lee CS, Kim SH. 2020. Anti-inflammatory and anti-osteoporotic potential of Lactobacillus plantarum A41 and L. fermentum SRK414 as probiotics. Probiotics Antimicrob. Proteins 12: 623-634.   DOI
34 Kim H, Jung BJ, Jung JH, Kim JY, Chung SK, Chung DK. 2012. Lactobacillus plantarum lipoteichoic acid alleviates TNF-α-induced inflammation in the HT-29 intestinal epithelial cell line. Mol. Cells 33: 479-486.   DOI
35 Buchholz KR, Stephens RS. 2008. The cytosolic pattern recognition receptor NOD1 induces inflammatory interleukin-8 during Chlamydia trachomatis infection. Infect. Immun. 76: 3150-3155.   DOI
36 Kohno M, Tanimura S, Ozaki K. 2011. Targeting the extracellular signal-regulated kinase pathway in cancer therapy. Biol. Pharm. Bull. 12: 1781-1784.   DOI
37 Li SC, Hsu WF, Chang JS, Shih CK. 2019. Combination of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis shows a stronger anti-inflammatory effect than individual strains in HT-29 cells. Nutrients 11: 969.   DOI