• 한국식품저장유통학회지
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• 제14권5호
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• pp.481-486
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• 2007

본 연구는 감마선 조사와 실크 펩타이드 병용처리가 세균의 방사선 감수성 및 햄버거 패티의 저장 안전성에 미치는 영향을 평가하기 위해 실시되었다. 멸균된 햄버거 패티에 접종된 4종의 세균에 대한 감마선 $D_{10}$ 값은 E. coli 0.25 kGy, L. ivanovii 0.50 kGy, S. typhimurium 0.55 kGy, C. sporogenes 1.35 kGy 이였다. 실크 펩타이드 5% 첨가 햄버거 패티에 접종된 S. typhimurium의 $D_{10}$ 값은 대조구의 0.558 kGy에서 0.53 kGy로 6% 낮아지는 것으로 나타났다. 한편, $30^{\circ}C$ 가속 저장조건에서 햄버거 패티의 미생물 생육도를 측정한 결과 7 kGy이상의 감마선 조사에 의해 저장기간 동안 미생물이 검출되지 않았으며, 실크 펩타이드 7% 첨가시 대조구에 비해 약 1 log cycle의 미생물 생육 감소를 확인하였다. 따라서 실크 펩타이드 첨가는 방사선 조사와 같이 다른 식품 살균기술과 함께 사용한다면 식품의 초기오염 미생물을 제어하여 저장 안정성을 확보하는데 도움이 되는 것으로 판단되었다.

• The Plant Pathology Journal
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• 제31권2호
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• pp.176-180
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• 2015

Postharvest diseases cause losses in a wide variety of crops around the world. Irradiation, a useful nonchemical approach, has been used as an alternative treatment for fungicide to control plant fungal pathogens. For a preliminary study, ionizing radiations (gamma, X-ray, or e-beam irradiation) were evaluated for their antifungal activity against Botrytis cinerea, Penicillium expansum, and Rhizopus stolonifer through mycelial growth, spore germination, and morphological analysis under various conditions. Different fungi exhibited different radiosensitivity. The inhibition of fungal growth showed in a dose-dependent manner. Three fungal pathogens have greater sensitivity to the e-beam treatment compared to gamma or X-ray irradiations. The inactivation of individual fungal-viability to different irradiations can be considered between 3-4 kGy for B. cinerea and 1-2 kGy for P. expansum and R. stolonifer based on the radiosensitive and radio-resistant species, respectively. These preliminary data will provide critical information to control postharvest diseases through radiation.

• Nuclear Engineering and Technology
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• 제54권9호
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• pp.3398-3402
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• 2022

We fabricated a 15 m long position-sensitive plastic scintillation optical fiber (PSOF) detector consisting of a PSOF, two photomultiplier tubes, four fast amplifiers, and a digitizer. A single PSOF was used as a sensing part to estimate the gamma-ray source position, and ¹³⁷Cs, an uncollimated solid-disk-type radioactive isotope, was used as a gamma-ray emitter. To improve the sensitivity, accuracy, and measurement time of a PSOF detector compared to those of previous studies, the performance of the amplifier was optimized, and the digital signal processing (DSP) was newly designed in this study. Moreover, we could measure very low dose rates of gamma-rays with high sensitivity and accuracy in a very short time using our proposed PSOF detector. The results of this study indicate that it is possible to accurately and quickly locate the position of a very low dose rate gamma-ray source in a wide range of contaminated areas using the proposed position-sensitive PSOF detector.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.181-184
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• 2004

This study was conducted to investigate the adequate irradiation dose to eliminate harmful fungi inoculated if beef jerky with a 10% higher moisture content and improved textural property. Aspergillus flavus (approximately $10^6\;CFU/cm^2$) was tested in broth, spore suspension, and inoculated jerky. $D_{10}$ values of A. flavus were 0.36 kGy in the broth and suspension, and 0.47 kGy in the jerky. The results indicate that gamma irradiation can be effectively used to control the fungus growth in beef jerky with an improved quality and higher moisture content.

• Nuclear Engineering and Technology
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• 제43권6호
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• pp.567-572
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• 2011

A sensitivity analysis of the methods used to evaluate the transport properties of a CdZnTe detector was performed using two different radiations (${\alpha}$ particle and gamma-ray) emitted from an $^{241}Am$ source. The mobility-lifetime products of the electron-hole pair in a planar CZT detector ($5{\times}5{\times}2\;mm^3$) were determined by fitting the peak position as a function of biased voltage data to the Hecht equation. To verify the accuracy of these products derived from ${\alpha}$ particles and low-energy gamma-rays, an energy spectrum considering the transport property of the CZT detector was simulated through a combination of the deposited energy and the charge collection efficiency at a specific position. It was found that the shaping time of the amplifier module significantly affects the determination of the (${\mu}{\tau}$) products; the ${\alpha}$ particle method was stabilized with an increase in the shaping time and was less sensitive to this change compared to when the gamma-ray method was used. In the case of the simulated energy spectrum with transport properties evaluated by the ${\alpha}$ particle method, the peak position and tail were slightly different from the measured result, whereas the energy spectrum derived from the low-energy gamma-ray was in good agreement with the experimental results. From these results, it was confirmed that low-energy gamma-rays are more useful when seeking to obtain the transport properties of carriers than ${\alpha}$ particles because the methods that use gamma-rays are less influenced by the surface condition of the CZT detector. Furthermore, the analysis system employed in this study, which was configured by a combination of Monte Carlo simulation and the Hecht model, is expected to be highly applicable to the study of the characteristics of CZT detectors.

• Journal of Radiation Protection and Research
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• 제16권2호
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• pp.1-6
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• 1991

상온에서 사용할 수 있는 감마선 검출 소자로서 $HgI_2$ 소자의 응용성을 판단하기 위하여 기상 성장법으로 $HgI_2$ 단결정을 성장시켰고 이를 이용하여 검출 소자를 제작한 후 감마선 검출 특성을 조사하였다 성장된 단결정의 비저항과 전하 운반자 포획 밀도는 상온에서 각각 $10^{11}{\Omega}\;cm$와 $1.8{\times}10^{14}/cm^3$였으며 단결정의 Photoluminescence를 측정한 결과 성장된 $HgI_2$ 단결정의 밴드갭의 온도계수는 20K부터 77K에서 $-1.53{\times}10^{-4}eV/K$였다. 감마선 검출실험 결과 제작된 $HgI_2$ 검출 소자는 상온에서 우수한 계수 특성과 시간에 대한 선형적 축적 계수 특성을 나타내었으나 온도변화에 따라 계수의 특성의 변화는 심하였다.

• Journal of Radiation Protection and Research
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• 제29권4호
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• pp.257-261
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• 2004

핀홀 감마카메라의 민감도를 향상시키기 위하여 픽셀화된 CsI(Tl) 섬광체와 위치민감형 광증배관을 이용하여 부호화 구경 감마카메라를 개발하였다. Rounded-hole로 이루어진 $13{\times}11$ 픽셀구조의 개조된 uniformly reductant array (URA) 가 계측기의 공간해상도를 고려하여 부호화된 마스크로 선택되었다. 부호화된 구경 카메라와 핀홀 카메라의 성능을 비교하기 위하여 Tc-99m 소스의 여러 가지 형태를 이용하여 테스트 하였으며, 신호 대 잡음비 또는 민감도의 향상에 대한 카메라의 성능을 분석하였다. 그 결과 공간해 상도에 있어서 약간의 저하가 있었으나 영상의 질은 매우 향상되었다. 비록 카메라의 개발과 테스트가 저에너지 영역에서 이루어졌지만, 부호화 구경 카메라의 구상은 방사성 물질 감시 그리고 다른 응용들에 있어서도 효과적으로 사용되어질 수 있을 것이다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7103-7110
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• 2015

The present study was conducted to investigate the effect of ${\gamma}$-radiation alone or combined with a cytotoxic drug, simvastatin, on viability and cell cycling of a myeloma cell line. P3NS1 myeloma cells were treated with the selected dose of simvastatin ($0.1{\mu}M/l$) 24 hours prior to ${\gamma}$-irradiation (0.25, 0.5 and 1Gy). The cell viability, induction of apoptosis, cell death, cell cycling, generation of ROS, and expression of P53, Bax, Bcl2, caspase3, PARP1 and Fas genes were estimated. The results indicated that simvastatin ($0.1{\mu}M/l$) treatment for 24 hours prior to ${\gamma}$-irradiation increased cell death to 37.5% as compared to 4.81% by radiation (0.5Gy) alone. It was found that simvastatin treatment before irradiation caused arrest of cells in G0/G1 and G2/M phases as assessed using flow cytometry. Interestingly, simvastatin treatment of P3NS1 cells increased the intracellular ROS production and decreased antioxidant enzyme activity with increased P53, Bax and Caspase3 gene expression while that of Bcl2 was decreased. Consequently, our results indicated that pre-treatment with simvastatin increased radio sensitivity of myeloma tumor cells in addition to apoptotic effects through an intrinsic mitochondrial pathway.

• 방사선산업학회지
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• 제8권2호
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• pp.111-121
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• 2014

This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leaf mustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated with gamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration to enlarge the characteristic morphological variations were also separately investigated. The survival rates of S-28 only linearly decreased with increasing the gamma-ray dose. The overall growth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. The reduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those of S-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 and S-27 showed no significant differences by EMS treatment, while the growth of all three lines were significantly decreased. The reduction concentration 50 in S-14 could not be determined, demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, while those in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseed lines possess higher sensitivity to EMS than S-14. Various morphological characteristics of $M_1$ generation obtained from mutagen treatment were elaborately investigated for further maintenance of $M_2$ generation. In $M_2$ generation variants showing short stem, yellow color in seed coat, chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially useful mutants for breeding.

• 미생물학회지
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• 제37권4호
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• pp.305-311
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• 2001

다양한 식물병원성 진균을 방제하기 위하여 온천수로부터 1종의 동물병원성 진균과 12종의 식물병원성 진균에 항진균 활성을 갖는 고온성 세균을 분리하였으며 Bacillus subtilis YS1으로 동정되었다. 분리 균주의 감마선($Co^{60}$)에 대한 감수성 조사 결과, $D_{10}$ value는 2.08 kGy로 20 kGy 의 방사선량에서도 생존 가능한 방사선 저항성 균주였다. 방사선 조사를 이용하여 5종의 돌연변이체를 유기하였으며, 이 중 B. subtilis YS1-1009 돌연변이체는 Botryoshaeria dothidea에 대한 항진균 활성의 증가 및 4종의 식물병원성 진균에 대하여 항진균 활성이 감소하는 종특이적 항진균 활성 변화를 나타내었다. 또한 상용농약인 tebuconazole에 대하여 저항성을 나타내었으며 17종의 다른 농약에 대해서도 전체적으로 야생형 균주에 비해 저항성이 증가하였다. B. subtilis YS1-1006 돌연변이체는 copper hydroxide에 저항성을 나타내었으며, B. subtilis YS67 돌연변이체는 항진균 활성이 소실된 tryptophan 및 proline 또는 uracil 및 arginine 영양요구성 돌연변이체였다. 이러한 결과로부터 극한 환경에서 분리한 본 분리균주는 환경친화적으로 진균에 의한 식물병을 방제할 수 있으며 방사선을 이용한 돌연변이체 유기는 기능성 균주의 개량이나 작용기작 연구를 위한 돌연변이체의 획득에 매우 효과적인 방법이라 사료된다.