• 대한치과보철학회지
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• 제54권3호
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• pp.203-209
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• 2016

목적: 치주조직재생을 위해서는 치주인대세포의 증식 및 이주를 촉진시키는 것이 필수적이나 현재까지 이것을 만족시키는 재생술식은 없다. 최근 법랑기질유도체(enamel matrix derivatives)가 치주조직 재생 술식에 적용되고 있으나 그 기전에 대해서는 완전히 알려지지 않았다. 따라서 본 연구의 목적은 법랑기질유도체가 치주인대세포의 증식과 성장인자 발현에 미치는 영향에 대하여 알아보고자 함이다. 재료 및 방법: 건강한 성인의 제 3 대구치로부터 치주인대세포를 추출한 후, 법랑기질유도체(Emdogain (Biora, Malmo, Sweden))의 농도가 각각 0, 12.5, 25, 50, 100, and $200{\mu}g/mL$인 배지에서 배양시켰다. 치주인대 세포증식과 알칼리성 인산분해효소(alkaline phosphatase) 활성도를 측정하고, 발현되는 성장인자를 평가하였다. 결과: 치주인대세포의 세포증식은 $25{\mu}g/mL$이상의 농도의 법랑기질유도체를 첨가한 군에서, 알칼리성 인산분해효소 활성도는 $50{\mu}g/mL$의 법랑기질유도체를 첨가한 군에서 각각 유의하게 증가하였고, $50{\mu}g/mL$의 법랑기질유도체를 첨가한 군에서 치주인대세포의 VEGF (vascular endothelial growth factor)와 TGF(transforming growth factor)-${\beta}$의 발현이 유의하게 증가하였다. 결론: 법랑기질 유도체는 인간 치주인대세포의 세포증식과 알칼리성 인산분해효소 활성을 증진시키고, VEGF와 TGF-${\beta}$등 성장인자의 발현을 촉진함으로써 치주조직 재생에 기여할 수 있을 것이다.

• 대한수의학회지
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• 제34권3호
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• pp.457-463
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• 1994

Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{\mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{\pm}99.79g$) and 35 days($1,252.84{\pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{\pm}40.22g,\;1,111.32{\pm}153.67g$). The liver and kidney weights on 35 days$(35.24{\pm}5.18g,\;11.05{\pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{\pm}4.04g,\;10.01{\pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{\pm}1.69ng/ml$, $3.75{\pm}0.62g/dl$ respectively compared with control group $45.70{\pm}1.64ng/ml$, $2.32{\pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum.

• 호남수학학술지
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• 제1권1호
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• pp.43-49
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• 1979

G를 유한군으로, C를 모든 복소수체로 가정하고, V를 C상에서의 유한차원 벡터공간이라 하자. V상에서의 G의 사영적 표시는, X, $y{\epsilon}G$이고 ${\alpha}:\;G{\times}G{\rightarrow}C$를 Facto set이라 할 때 $T(x)T(y)=T(xy){\alpha}(x,y)$이 되는 함수 $T=\;G{\rightarrow}GL(V)$를 말한다. 본 논문의 목적은 군에 대한 Extension theory를 사용해서, G상의 factor set들의 동치류들은 G의 Second Cohomology group과 동형이라는 것을 증명하는 것이다.

• 대한의생명과학회지
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• 제8권4호
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• pp.229-234
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• 2002

Chlorella is rich in chlorella growth factor (CGF). A review of the literature has described that CGF improves the capability of a Th1-based immunity, anticancer, antioxidant antibacterial activity, growth promotion, wound healing and so on, but has not studied the effect for the metabolism and the proliferation of human skin keratinocyte. The aim of this study was to examine the effect of metabolism and the proliferation of human skin keratinocyte in vitro. CGF was extracted with an autoclaving method which is a modified hot-water extraction method from dried chlorella and conformed by means of absorbance 0.22 at 260 nm. We have measured the extracellular acidification rate (ECAR) of the CGF by Cytosensor$^{\circledR}$ Microphysiometer and evaluated responsiveness depending upon the dosage on the HaCaT cell. The ECAR for the concentrations of 0.15, 1.5, 15, 150 $\mu\textrm{g}$/ml of CGF increased as a 103.6, 128.2, 149.0 and 423.9%, respectively compared to control (0.0 $\mu\textrm{g}$/ml, 100% ECAR). The ECAR for ErbBl tyrosine kinase inhibited by 4-anilinoquinazolines, $C_{16}$H$_{14}$BrN$_3$O$_2$.HCl on tile HaCaT cells with the amounts of 10 $\mu\textrm{g}$/ml of the CCF compared with 100 $\mu\textrm{g}$/ml of rhEGF. The conclusion of the study is that CGF might increase human epidermal keratinocyte proliferation through the interaction between the epidermal growth factor receptor and itself.

• 방사성폐기물학회지
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• 제13권4호
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• pp.295-300
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• 2015

HPGe 검출기를 이용하여 밀도가 다양한 환경시료에 대한 정밀 분석시 정확한 분석을 위해서는 밀도보정인자가 필요하다. 밀도에 대한 보정인자를 구하기 위해서 본 연구에서는 몬테카를로 코드인 MCNPX 코드를 사용하여 크리스털의 높이, 지름 및 코어의 크기와 같은 특성이 다른 세 대의 p-type HPGe 검출기를 모사하고 밀도 $1g/m^3$의 교정용 표준시료를 이용하여 모델링을 검증하였다. 검증을 통하여 모델링을 확정한 후 0.3, 0.6, 0.9, 1.0, 1.2, $1.5g/m^3$ 밀도를 가진 샘플에 대한 효율을 시뮬레이션하고 밀도보정인자를 도출하였다. 도출된 각 검출기에 대한 밀도보정인자를 비교하였을 때 전 에너지 범위에서 그 차이가 거의 없음을 확인하였으며 이는 검출기의 크리스털과 같은 주요 특성에 대해 밀도보정인자가 독립적임을 의미한다.

• Journal of applied mathematics & informatics
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• 제31권5_6호
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• pp.669-676
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• 2013

Let $a$ and $b$ be two even integers with $2{\leq}a<b$, and let k be a nonnegative integer. Let G be a graph of order $n$ with $n{\geq}\frac{(a+b-1)(a+b-2)+bk-2}{b}$. A graph G is called an ($a,b,k$)-critical graph if after deleting any $k$ vertices of G the remaining graph of G has an [$a,b$]-factor. In this paper, it is proved that G is an ($a,b,k$)-critical graph if $${\mid}N_G(X){\mid}&gt;\frac{(a-1)n+{\mid}X{\mid}+bk-2}{a+b-1}$$ for every non-empty independent subset X of V (G), and $${\delta}(G)>\frac{(a-1)n+a+b+bk-3}{a+b-1}$$. Furthermore, it is shown that the result in this paper is best possible in some sense.

• 한국균학회지
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• 제21권3호
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• pp.235-245
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• 1993

The symbiotic properties of arbuscular mycorrhizal fungi(AMF) such as the absence and/or presence of symbiosis, spore density, and six factors of phycochemical properties of soil were investigated in the rhizosphere of seven sand dune plants and three control plants around Kum river. The infection of AMF was confirmed in all plants. Three genera, nine species of the AMF spores were identified in the sand dune; Ac. scrobiculata, G. aggregatum, G. convolutum, G. diaphanum, G. dimophicum, G. geosporum, G. vesiculiferum, G. tortuosum, S. pachycaulis. All of them were also found in the control, but S. pachycaulis were only separated in the sand dune. In the control, three genera, four species were identified; G. flavisporum, Gi. margarita, Sc. gregaria. Sc. persica. The species specificity was not found between the sand dune plant and AMF. However, the AMF was thought to be correlated with the soil factors. The spore density was stimulated with increase of the organic matter and the nitrogen content, but inhibited with the water content and the phosphorus content. In respect of the seasonal factor, the spore density was increased in the late growth period of plants.

• Journal of Ginseng Research
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• 제43권2호
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• pp.282-290
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• 2019

Background: Ginsenoside Rg3 (G-Rg3) is the major bioactive ingredient of Panax ginseng and has many pharmacological effects, including antiadipogenic, antiviral, and anticancer effects. However, the effect of G-Rg3 on mast cell-mediated allergic inflammation has not been investigated. Method: The antiallergic effects of G-Rg3 on allergic inflammation were evaluated using the human and rat mast cell lines HMC-1 and RBL-2H3. Antiallergic effects of G-Rg3 were detected by measuring cyclic adenosine monophosphate (cAMP), detecting calcium influx, and using real-time reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and in vivo experiments. Results: G-Rg3 decreased histamine release from activated mast cells by enhancing cAMP levels and calcium influx. Proinflammatory cytokine production was suppressed by G-Rg3 treatment via regulation of the mitogen-activated protein kinases/nuclear factor-kappa B and receptor-interacting protein kinase 2 (RIP2)/caspase-1 signaling pathway in mast cells. Moreover, G-Rg3 protected mice against the IgE-mediated passive cutaneous anaphylaxis reaction and compound 48/80-induced anaphylactic shock. Conclusion: G-Rg3 may serve as an alternative therapeutic agent for improving allergic inflammatory disorders.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.842-850
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• 2002

Tissue factor (TF, tissue thromboplastin or coagulation factor III) accelerates the blood clotting, activating both the intrinsic and the extrinsic pathways to serve as a cofactor. In order to isolate TF inhibitors from the fruits of Chaenomeles sinensis, an activity-guided purification utilizing a bio-assay method of prothrombin time prolongation, was carried out to yield five active flavoniods such as hovetrichoside C (1) ($IC_{50}$ = 14.0 $\mu$g), luteolin-7-Ο-$\beta$-D-glucuronide (3) ($IC_{50}$ = 31.9$\mu$g), hyperin (4) ($IC_{50}$ = 20.8 $\mu$g), avicularin (6) ($IC_{50}$ = 54.8 $\mu$g) and quercitrin (10) ($IC_{50}$ = 135.7 $\mu$g), along with other inactive compounds such as ($\pm$)-(2E,4E)-Ο-$\beta$-D-glucopyranosyl-4'-hydroxy-$\beta$-ionylideneacetic acid ester (2), genistein-7-Ο-$\beta$-D-glucopyranoside (5), luteolin-3'-methoxy-4'-Ο-$\beta$-D-glucopyranoside (7), luteolin-7-Ο-$\beta$-D-glucuronide methyl ester (8), tricetin-3'-methoxy-4'-Ο-$\beta$-D-glucopyranoside (selagin-4'-Ο-$\beta$-D-glucopyranoside) (9), (-)-epicatechin (11), luteolin-4'-Ο-$\beta$-D-glucopyranoside (12) and apigenin-7-Ο-$\beta$-D-glucuronide methyl ester (13). The structures of the isolated compounds were elucidated through spectral analysis. Among them, compounds 1 to 9, 12 and 13 were isolated for the first time from the fruits of this plant and the compound 9 is a new flavonoid.

• 한국환경생태학회지
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• 제29권1호
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• pp.29-45
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• 2015

Water chemistry and fish community, based on fish compositions and ecological characteristics(trophic/tolerance guilds and condition factor), were compared in Gucheon Reservoir($G_cR$) and Yeoncho Reservoir($Y_cR$). Chemical parameters of water quality such as BOD, COD, nutrient(N, P) and suspended solids indicated that water quality was better in the $Y_cR$ than $G_cR$, and the temporal variability in seasonal and interannual patterns were greater in the $Y_cR$. The greater variability was mainly attributed to intense dilutions of reservoir water by Asian monsoon rain during July-August. Fish guild analysis indicated that species diversity was higher in the $G_cR$ than the $Y_cR$, and that the proportion of tolerant and omnivore species were greater in the $Y_cR$. Regression analysis of body weight-total length showed that the regression coefficient(b value) was lower in the $G_cR$(2.15 ~ 2.40) than the $Y_cR$(2.59 ~ 3.14). Condition factor(K) of fish against the total length showed negative slope of Zacco temminckii, Carassius auratus, Pseudorasbora parva and Rhinogobius brunneus population in the $G_cR$, and a positive slope of Carassius auratus and Rhinogobius brunneus population in $Y_cR$. Overall, our data suggest that the growth of the fish populations, based on the length-weight relations and condition factor, reflected the trophic regime of nutrients and organic matter.